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31. 发明申请

WO2012061307A1 PORTABLE DEVICE FOR EX VIVO STIMULATION OF WHOLE BLOOD 审中-公开
标题翻译：便携式装置用于全血淋巴细胞浸润
公开(公告)号：WO2012061307A1
公开(公告)日：2012-05-10
申请号：PCT/US2011/058624
申请日：2011-10-31
申请人： HITACHI CHEMICAL CO., LTD , HITACHI CHEMICAL RESEARCH CENTER, INC. , MITSUHASHI, Masato , MURAKAMI, Taku
发明人： MITSUHASHI, Masato , MURAKAMI, Taku
IPC分类号： A61B5/15 , A61K35/14
CPC分类号： A01N1/0284 , A01N1/0252 , A61B5/15003 , A61B5/150206 , A61B5/150305 , A61B5/150389 , A61B5/150755 , A61B5/153 , A61B5/154 , A61K35/14
摘要： Disclosed are methods, device kits, and systems for improved quantification of mRNA from whole blood. More particularly, the devices and kites related thereto are useful for the controlled and repeatable ex vivo stimulation of whole blood.
摘要翻译：公开了用于改进来自全血的mRNA定量的方法，装置试剂盒和系统。更具体地，与其相关的装置和风筝对于全血的受控和可重复的离体刺激是有用的。

32. 发明申请

WO2011140125A1 METHODS OF CHARACTERIZING HOST RESPONSIVENESS TO INTERFERON BY EX VIVO INDUCTION OF INTERFERON-RESPONSIVE MARKERS 审中-公开
标题翻译：通过EXVIVO诱导干扰素标记表征干扰素对宿主的反应的方法
公开(公告)号：WO2011140125A1
公开(公告)日：2011-11-10
申请号：PCT/US2011/035045
申请日：2011-05-03
申请人： HITACHI CHEMICAL CO., LTD. , HITACHI CHEMICAL RESEARCH CENTER, INC. , THE CLEVELAND CLINIC FOUNDATION , MITSUHASHI, Masato , BORDEN, Ernest, C.
发明人： MITSUHASHI, Masato , BORDEN, Ernest, C.
IPC分类号： C12Q1/00 , G01N33/48
CPC分类号： C12Q1/6876 , C12Q1/6883 , C12Q1/6886 , C12Q2600/106 , C12Q2600/158
摘要： Embodiments of the invention relate generally to ex vivo methods of quantifying expression of interferon responsive genes and characterizing an individual's potential responsiveness to interferon administration. Certain embodiments relate to methods to monitor the efficacy of ongoing interferon therapy by evaluating expression of interferon responsive genes before and after interferon administration.
摘要翻译：本发明的实施方案一般涉及定量干扰素应答基因的表达的离体方法，并表征个体对干扰素施用的潜在反应性。某些实施方案涉及通过评估干扰素施用前后的干扰素应答基因的表达来监测正在进行的干扰素疗法的功效的方法。

33. 发明申请

WO2009070442A3 FC RECEPTOR-MEDIATED TUMOR NECROSIS FACTOR SUPERFAMILY MRNA EXPRESSION IN PERIPHERAL BLOOD LEUKOCYTES 审中-公开
公开(公告)号：WO2009070442A3
公开(公告)日：2009-06-04
申请号：PCT/US2008/083136
申请日：2008-11-11
申请人： HITACHI CHEMICAL CO. LTD. , HITACHI CHEMICAL RESEARCH CENTER, INC , MITSUHASHI, Masato , ENDO, Katsuya , OBARA, Kazuhiko , IZUTSU, Hiroshi , OHTA, Shuji
发明人： MITSUHASHI, Masato , ENDO, Katsuya , OBARA, Kazuhiko , IZUTSU, Hiroshi , OHTA, Shuji
IPC分类号： C12Q1/68 , C12P19/34
摘要： A method for predicting patient responsiveness to rheumatoid arthritis treatments involving altering expression of tumor necrosis factor superfamily ("TNFSF")-2, TNFSF-8, or TNFSF- 15 is disclosed. A method for monitoring the effectiveness of such therapy is also disclosed. Furthermore, a method of screening compounds for use in the treatment of rheumatoid arthritis is disclosed. A method of monitoring the disease state over time in rheumatoid arthritis patients is also disclosed.

34. 发明申请

WO2007133551A2 METHOD FOR TESTING DRUG SENSITIVITY IN SOLID TUMORS BY QUANTIFYING MRNA EXPRESSION IN THINLY-SLICED TUMOR TISSUE 审中-公开
标题翻译：通过在薄片肿瘤组织中定量检测MRNA表达来测定固体肿瘤中药物灵敏度的方法
公开(公告)号：WO2007133551A2
公开(公告)日：2007-11-22
申请号：PCT/US2007/011121
申请日：2007-05-08
申请人： HITACHI CHEMICAL CO., LTD. , HITACHI CHEMICAL RESEARCH CENTER, INC. , MITSUHASHI, Masato , OBARA, Kazuhiko
发明人： MITSUHASHI, Masato , OBARA, Kazuhiko
IPC分类号： F02B19/00 , F02F3/00
CPC分类号： C12Q1/6886 , C12Q2600/106 , C12Q2600/136
摘要： A method is disclosed for assaying the sensitivity of neoplastic tissue to therapeutic agents, and in particular, for the quantification of pro-apoptotic marker mRNA expression in cells obtained from thinly-sliced living tumor tissue in such methods. The method may comprise ascertaining a particular apoptosis marker mRNA for an individual tumor or tumor type as well as exposure of thin-sliced live cancer tissues from the individual tumor to candidate chemotherapeutic drug regimes in vitro, followed by an assessment of the level of the marker mRNA in the tissue.
摘要翻译：公开了一种用于测定肿瘤组织对治疗剂的敏感性的方法，特别是用于定量在这样的方法中从薄切片的活体肿瘤组织获得的细胞中促凋亡标记物mRNA表达。该方法可以包括确定个体肿瘤或肿瘤类型的特定细胞凋亡标志物mRNA，以及将来自个体肿瘤的薄切活活组织从体外暴露于候选化疗药物方案，随后评估标志物的水平组织中的mRNA。

35. 发明申请

WO2006133399A1 METHOD FOR PREDICTING IMMUNE RESPONSE TO NEOPLASTIC DISEASE BASED ON mRNA EXPRESSION PROFILE IN NEOPLASTIC CELLS AND STIMULATED LEUKOCYTES 审中-公开
标题翻译：基于神经细胞和刺激白血病细胞中mRNA表达谱的免疫应答预防方法
公开(公告)号：WO2006133399A1
公开(公告)日：2006-12-14
申请号：PCT/US2006/022427
申请日：2006-06-08
申请人： HITACHI CHEMICAL RESEARCH CENTER, INC. , HITACHI CHEMICAL CO., LTD. , MITSUHASHI, Masato
发明人： MITSUHASHI, Masato
IPC分类号： C12Q1/70 , G01N33/53 , G01N33/00
CPC分类号： G01N33/505 , C12Q1/6806 , C12Q1/6886 , C12Q2600/118 , C12Q2600/158 , G01N33/574
摘要： Tumor necrosis factor (TNF) is capable of inducing apoptosis by interacting with specific TNF receptors on the surface of cancer cells. Because multiple members of TNF ligand and receptor are present within each superfamily, over 300 different ligand-receptor combinations exist. Activated blood leukocytes produce TNF as part of the immune response to cancer, as well as producing chemokines to attract other leukocytes to the site. A method is disclosed of detecting significant induction of a variety of TNF superfamily subtype and chemokine mRNAs in blood leukocytes when whole blood is exposed to heat-aggregated IgG or anti-T cell receptor antibodies as a model of immune system interactions. Substantial individual-to-individual variation is observed in TNF subtypes and chemokines induced. Since peripheral blood leukocytes are the supply of anti-cancer immune cells, the quantitation of ex vivo inducibility of appropriate TNF ligands and chemokines in blood will be useful in individualized cancer immunotherapy. If the tumor mass is small, such as with early invisible metastatic lesions, appropriate TNF assaults may be sufficient to prevent relapse.
摘要翻译：肿瘤坏死因子（TNF）能够通过与癌细胞表面的特异性TNF受体相互作用诱导细胞凋亡。由于TNF配体和受体的多个成员存在于每个超家族中，所以存在超过300种不同的配体 - 受体组合。活化的血液白细胞产生TNF作为对癌症的免疫应答的一部分，以及产生趋化因子以吸引其他白细胞到该部位。公开了当将全血暴露于作为免疫系统相互作用的模型的热聚集的IgG或抗T细胞受体抗体时，检测血白细胞中各种TNF超家族亚型和趋化因子mRNA的显着诱导的方法。在TNF亚型和趋化因子诱导中观察到显着的个体与个体差异。由于外周血白细胞是抗癌免疫细胞的供应，血液中适当的TNF配体和趋化因子的离体诱导能力的定量将可用于个体化的癌症免疫治疗。如果肿瘤质量小，如早期隐形转移性病变，则适当的TNF攻击可能足以防止复发。

36. 发明申请

WO2005067646A3 PRIMERS AND PROBES FOR THE DETECTION OF HIV 审中-公开
标题翻译：检测艾滋病毒的前提和探索
公开(公告)号：WO2005067646A3
公开(公告)日：2006-08-10
申请号：PCT/US2005000504
申请日：2005-01-07
申请人： HITACHI CHEMICAL RES CT INC , HITACHI CHEMICAL CO LTD , MITSUHASHI MASATO
发明人： MITSUHASHI MASATO
IPC分类号： C12P19/34 , C07H21/04 , C12N9/00 , C12Q1/68
CPC分类号： C12Q1/686 , C12Q2561/101
摘要： Provided herein are primer/probe sets useful for detecting HIV (HIV-1) in a test sample. The primer/probe sets can be employed according to nucleic acid amplification procedures including PCR, real-time quantitative PCR, or RT-PCR. The primer/probe sets can also be provided in the form of a kit with other reagents for performing a nucleic acid amplification reaction.
摘要翻译：本文提供了可用于检测测试样品中HIV（HIV-1）的引物/探针组。可以根据包括PCR，实时定量PCR或RT-PCR的核酸扩增方法使用引物/探针组。引物/探针组还可以以试剂盒的形式提供用于进行核酸扩增反应的其它试剂。

37. 发明申请

WO02066637A8 METHOD FOR COLLECTING AND USING NUCLEAR MRNA 审中-公开
标题翻译：收集和使用核MRNA的方法
公开(公告)号：WO02066637A8
公开(公告)日：2003-10-30
申请号：PCT/US0149498
申请日：2001-10-25
申请人： HITACHI CHEMICAL RES CT INC , HITACHI CHEMICAL CO LTD , HITACHI LTD , MITSUHASHI MASATO
发明人： MITSUHASHI MASATO
IPC分类号： G01N33/53 , C12N15/09 , C12N15/10 , C12Q1/68 , G01N33/566 , G01N37/00
CPC分类号： C12Q1/6809 , C12N15/1017
摘要： Conventional gene expression systems and purification methods target mRNA present throughout the entire cell and, thus, their results show the sum of newly transcribed mRNA as well as digested mRNA. Thus, it was previously difficult to detect expression of new mRNA under conditions that digestion of mRNA is in progress. Further, when a large amount of mRNA is already present, it is difficult to detect a slight change in the quantity of mRNA, and thus sensitivity is low. A new method is developed by focusing on the fact that newly synthesized mRNA is confined inside the nucleus. Cells are captured on a membrane and then treated by a cell membrane permeation solution such as NP-40, thereby increasing permeability to cell membranes. After the cytoplasm is washed, nuclei are dissolved with a cell dissolving solution, and then mRNA can be successfully recovered from the thus-obtained solution. This method is very simple, and only 2-3 minutes extra time is required, but a large number of specimens can be treated and the method can be incorporated into an automated system. Thus, this method is likely to be considered to be a standard method of treating a sample for a gene expression analysis, particularly for RT-PCR.
摘要翻译：传统的基因表达系统和纯化方法靶向整个细胞中的mRNA，因此，它们的结果显示新转录的mRNA以及消化的mRNA的总和。因此，以前难以在正在进行mRNA消化的条件下检测新mRNA的表达。此外，当已经存在大量mRNA时，难以检测mRNA的量的轻微变化，因此灵敏度低。通过关注新合成的mRNA被限制在核内的事实来开发新的方法。将细胞捕获在膜上，然后用细胞膜渗透溶液如NP-40处理，从而增加细胞膜的通透性。细胞质洗涤后，用细胞溶解溶液溶解细胞核，然后从所得溶液中成功地回收mRNA。这种方法非常简单，只需要2-3分钟的时间，但可以处理大量的样本，并且该方法可以并入自动化系统。因此，该方法可能被认为是用于基因表达分析的标准方法，特别是对于RT-PCR。

38. 发明申请

WO2002066637A2 METHOD FOR COLLECTING AND USING NUCLEAR MRNA 审中-公开
标题翻译：收集和使用核MRNA的方法
公开(公告)号：WO2002066637A2
公开(公告)日：2002-08-29
申请号：PCT/US2001/049498
申请日：2001-10-25
申请人： HITACHI CHEMICAL RESEARCH CENTER, INC. , HITACHI CHEMICAL CO., LTD. , HITACHI, LTD. , MITSUHASHI, Masato
发明人： MITSUHASHI, Masato
IPC分类号： C12N15/10
CPC分类号： C12Q1/6809 , C12N15/1017
摘要： Conventional gene expression systems and purification methods target mRNA present throughout the entire cell and, thus, their results show the sum of newly transcribed mRNA as well as digested mRNA. Thus, it was previously difficult to detect expression of new mRNA under conditions that digestion of mRNA is in progress. Further, when a large amount of mRNA is already present, it is difficult to detect a slight change in the quantity of mRNA, and thus sensitivity is low. A new method is developed by focusing on the fact that newly synthesized mRNA is confined inside the nucleus. Cells are captured on a membrane and then treated by a cell membrane permeation solution such as NP-40, thereby increasing permeability to cell membranes. After the cytoplasm is washed, nuclei are dissolved with a cell dissolving solution, and then mRNA can be successfully recovered from the thus-obtained solution. This method is very simple, and only 2-3 minutes extra time is required, but a large number of specimens can be treated and the method can be incorporated into an automated system. Thus, this method is likely to be considered to be a standard method of treating a sample for a gene expression analysis, particularly for RT-PCR.
摘要翻译：传统的基因表达系统和纯化方法靶向整个细胞中的mRNA，因此，它们的结果显示新转录的mRNA以及消化的mRNA的总和。因此，以前难以在正在进行mRNA消化的条件下检测新mRNA的表达。此外，当已经存在大量mRNA时，难以检测mRNA的量的轻微变化，因此灵敏度低。通过关注新合成的mRNA被限制在核内的事实来开发新的方法。将细胞捕获在膜上，然后用细胞膜渗透溶液如NP-40处理，从而增加细胞膜的通透性。细胞质洗涤后，用细胞溶解溶液溶解细胞核，然后从所得溶液中成功地回收mRNA。这种方法非常简单，只需要2-3分钟的时间，但可以处理大量的样本，并且该方法可以并入自动化系统。因此，该方法可能被认为是用于基因表达分析的标准方法，特别是对于RT-PCR。

39. 发明申请

WO2002058848A1 ROBOT-FRIENDLY PCR PLATES 审中-公开
标题翻译：机器人友好的PCR板
公开(公告)号：WO2002058848A1
公开(公告)日：2002-08-01
申请号：PCT/US2002/002260
申请日：2002-01-24
申请人： HITACHI CHEMICAL CO., LTD. , HITACHI CHEMICAL RESEARCH CENTER, INC. , MITSUHASHI, Masato , SAITO, Takayuki , KAWAI, Hiromasa , TAKASE, Chikashi
发明人： MITSUHASHI, Masato , SAITO, Takayuki , KAWAI, Hiromasa , TAKASE, Chikashi
IPC分类号： B01L3/00
CPC分类号： B01L3/50851
摘要： The present invention relates to microtiter plates for use in automated systems. Specifically, the present invention relates to microtiter plates which are thermally stable under conditions required for polymerase chain reaction (PCR) experiments, while also maintaining the rigidity and dimensions necessary for mechanical manipulation in automated systems.
摘要翻译：本发明涉及用于自动化系统的微量滴定板。具体地，本发明涉及在聚合酶链式反应（PCR）实验所需的条件下热稳定的微量滴定板，同时还保持在自动化系统中机械操作所需的刚度和尺寸。

40. 发明申请

WO1994011837A1 OLIGOPROBE DESIGNSTATIONS: A COMPUTERIZED METHOD FOR DESIGNING OPTIMAL OLIGONUCLEOTIDE PROBES AND PRIMERS 审中-公开
标题翻译： OLIGOPROBE设计：一种用于设计最佳寡核苷酸探针和引物的计算机方法
公开(公告)号：WO1994011837A1
公开(公告)日：1994-05-26
申请号：PCT/US1993010507
申请日：1993-11-08
申请人： HITACHI CHEMICAL COMPANY, LTD. , MITSUHASHI, Masato , COOPER, Allan , WATERMAN, Michael , PEVZNER, Pavel
发明人： HITACHI CHEMICAL COMPANY, LTD.
IPC分类号： G06F15/42
CPC分类号： C12N15/10 , G06F19/20 , G06F19/22
摘要： There is disclosed herein an invention which relates to the fields of genetic engineering, microbiology, and computer science, that allows a user, whether a molecular biologist or a clinical diagnostician, to calculate and design extremely specific oligonucleotide sequences for DNA and mRNA hybridization procedures. The sequences designed with this invention may be used for medical diagnostic kits, DNA indentification, and potentially continuous monitoring of metabolic processes in human beings. The key features design oligonucleotide sequences based on the GenBank database of DNA and mRNA sequences and examine candidate sequences for specificity or commonality with respect to a user-selected experimental preparation. Two models are available: a Mismatch Model, that employs hashing and continuous seed filtration, and an H-site Model, that analyzes candidate sequences for their binding specificity relative to some known set of mRNA or DNA sequences. The preferred embodiment of this computerized design tool is written in the Borland C++ language and runs under Microsoft Windows on IBM compatible personal computers.
摘要翻译：本文公开了一种涉及遗传工程，微生物学和计算机科学领域的发明，其允许用户（无论是分子生物学家还是临床诊断学家）计算和设计用于DNA和mRNA杂交程序的特异性寡核苷酸序列。本发明设计的序列可用于医学诊断试剂盒，DNA鉴定和人类代谢过程的潜在连续监测。关键特征是基于DNA和mRNA序列的GenBank数据库的设计寡核苷酸序列，并检查相对于用户选择的实验制备的特异性或共同性的候选序列。有两种模型可用：使用散列和连续种子过滤的不匹配模型和H位点模型，它们相对于一些已知的一组mRNA或DNA序列分析其结合特异性的候选序列。该计算机化设计工具的优选实施例是用Borland C ++语言编写的，并且在IBM兼容的个人计算机上的Microsoft Windows TM下运行。

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