专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

21. 发明申请

WO2005013090A2 SYSTEMS AND METHODS FOR BIOPOLYMER ENGINEERING 审中-公开
标题翻译：生物聚合物工程系统与方法
公开(公告)号：WO2005013090A2
公开(公告)日：2005-02-10
申请号：PCT/US2004/024752
申请日：2004-07-30
申请人： DNA TWOPOINTO INC. , GUSTAFSSON, Claes , GOVINDARAJAN, Sridhar , MINSHULL, Jeremy, Stephen
发明人： GUSTAFSSON, Claes , GOVINDARAJAN, Sridhar , MINSHULL, Jeremy, Stephen
IPC分类号： G06F
CPC分类号： G06F19/18 , C07K16/1027 , C07K16/461 , C07K2317/76 , G06F19/16 , G06F19/24
摘要： Methods, computer systems, and computer program products for biopolymer engineering. A variant set for a biopolymer of interest is constructed by identifying, using a plurality of rules, a plurality of positions in the biopolymer of interest and, for each respective position in the plurality of positions, substitutions for the respective position. The plurality of positions and the substitutions for each respective position in the plurality of positions collectively define a biopolymer sequence space. A variant set comprising a plurality of variants of the biopolymer of interest is selected. A property of all or a portion of the variants in the variant set is measured. A sequence-activity relationship is modeled between (i) one or more substitutions at one or more positions of the biopolymer of interest represented by the variant set and (ii) the property measured for all or the portion of the variants in the variant set. The variant set is redefined to comprise variants that include substitutions in the plurality of positions that are selected based on a function of the sequence-activity relationship.
摘要翻译：方法，计算机系统和生物聚合物工程的计算机程序产品。通过使用多个规则来识别感兴趣的生物聚合物中的多个位置，并且对于多个位置中的每个相应位置，通过识别相应位置的替代来构建用于感兴趣的生物聚合物的变体。多个位置中的各个位置的多个位置和替换共同限定生物聚合物序列空间。选择包含感兴趣的生物聚合物的多个变体的变体组。测量变体集中全部或部分变体的属性。在（i）由变体组表示的感兴趣的生物聚合物的一个或多个位置处的一个或多个取代和（ii）针对变体组中的全部或部分变体测量的特性的序列活性关系进行建模。变体集被重新定义为包括基于序列活动关系的函数而选择的多个位置中的替换的变体。

22. 发明申请

WO2003078583A2 OPTIMIZATION OF CROSSOVER POINTS FOR DIRECTED EVOLUTION 审中-公开
标题翻译：用于指导性演变的优势点优化
公开(公告)号：WO2003078583A2
公开(公告)日：2003-09-25
申请号：PCT/US2003/007610
申请日：2003-03-10
申请人： MAXYGEN, INC. , MUNDORFF, Emily, C. , GOVINDARAJAN, Sridhar , GUSTAFSSON, Claes , MINSHULL, Jeremy, S.
发明人： GOVINDARAJAN, Sridhar , GUSTAFSSON, Claes , MINSHULL, Jeremy, S.
IPC分类号： C12N
CPC分类号： G06F19/22 , C40B50/02 , G06F19/16
摘要： Methods and devices for more efficiently engineering diversity into recombinant polypeptides and/or nucleic acids are provided herein. For example, a variety of methods of selecting and/or assessing potential crossover sites in an amino acid sequence or a nucleotide sequence are provided, as well as the resulting chimeric product sequences. These methods include, e.g., consideration of structural, functional and/or statistical data in the selection and assessment of sequences and crossover sites for use in recombination.
摘要翻译：本文提供了用于更有效地将多样性工程化成重组多肽和/或核酸的方法和装置。例如，提供了选择和/或评估氨基酸序列或核苷酸序列中的潜在交叉位点的各种方法，以及所得嵌合产物序列。这些方法包括例如考虑用于重组的序列和交换位点的选择和评估中的结构，功能和/或统计数据。

23. 发明申请

WO0123401A3 USE OF CODON-VARIED OLIGONUCLEOTIDE SYNTHESIS FOR SYNTHETIC SEQUENCE RECOMBINATION 审中-公开
标题翻译：用于合成序列重组的CODON-VARIED OLIGONUCLEOTIDE SYNTHESIS的使用
公开(公告)号：WO0123401A3
公开(公告)日：2001-12-06
申请号：PCT/US0026708
申请日：2000-09-28
申请人： MAXYGEN INC , WELCH MARK , NESS JON E , GUSTAFSSON CLAES , STEMMER WILLEM P C , MINSHULL JEREMY
发明人： WELCH MARK , NESS JON E , GUSTAFSSON CLAES , STEMMER WILLEM P C , MINSHULL JEREMY
IPC分类号： C12N15/10 , C12N15/11 , G06F19/00
CPC分类号： C12N15/1031 , B01J2219/00689 , C12N15/11
摘要： Methods of providing recombination libraries that include codon-varied oligonucleotide sequences are described. Codon-varied oligonucleotides are synthesized using trinucleotide or mononucleotide phosphoramidite sequences, and are derived from homologous or non-homologous nucleic acid sequences, or combinations of such sequences. Various methods of recombining codon-varied oligonucleotide sequences to expedite artificial evolution are also described. The present invention additionally relates to various integrated systems that are optionally used to automate these recombination methods.
摘要翻译：描述了提供包含密码子变化的寡核苷酸序列的重组文库的方法。使用三核苷酸或单核苷酸亚磷酰胺序列合成密码子多样的寡核苷酸，并衍生自同源或非同源核酸序列或这些序列的组合。还描述了重组密码子变化的寡核苷酸序列以加速人造进化的各种方法。本发明还涉及可选地用于使这些重组方法自动化的各种集成系统。

24. 发明申请

WO0175767A2 IN SILICO CROSS-OVER SITE SELECTION 审中-公开
标题翻译：在SILICO交叉点网站选择
公开(公告)号：WO0175767A2
公开(公告)日：2001-10-11
申请号：PCT/US0110231
申请日：2001-03-30
申请人： MAXYGEN INC , GUSTAFSSON CLAES , MINSHULL JEREMY , SELIFONOV SERGEY A , MUNDORFF EMILY , EMIG ROBIN , GOVINADARAJAN SRIDAR , STEMMER WILLEM P C , GIVER LORRAINE J , TOBIN MATTHEW , DEL CARDAYRE STEPHEN , PATTEN PHILLIP A
发明人： GUSTAFSSON CLAES , MINSHULL JEREMY , SELIFONOV SERGEY A , MUNDORFF EMILY , EMIG ROBIN , GOVINADARAJAN SRIDAR , STEMMER WILLEM P C , GIVER LORRAINE J , TOBIN MATTHEW , DEL CARDAYRE STEPHEN , PATTEN PHILLIP A
IPC分类号： G06F19/22 , G06F19/00
CPC分类号： G06F19/22
摘要： "In silico" nucleic acid and protein recombination methods, related integrated systems utilizing genetic operators and libraries made by in silico shuffling methods are provided, including design and selection of cross-over events in silico and corresponding nucleic acid synthesis.
摘要翻译：提供了“计算机”核酸和蛋白质重组方法，利用遗传算子和通过计算机混洗方法制作的文库的相关综合系统，包括计算机中交叉事件的设计和选择以及相应的核酸合成。

25. 发明申请

WO0042560A3 METHODS FOR MAKING CHARACTER STRINGS, POLYNUCLEOTIDES AND POLYPEPTIDES 审中-公开
标题翻译：用于制造字符串，多核苷酸和多聚体的方法
公开(公告)号：WO0042560A3
公开(公告)日：2000-11-23
申请号：PCT/US0001202
申请日：2000-01-18
申请人： MAXYGEN INC , SELIFONOV SERGEY A , STEMMER WILLEM P C , GUSTAFSSON CLAES , TOBIN MATTHEW , DEL CARDAYRE STEPHEN , PATTEN PHILLIP A , MINSHULL JEREMY
发明人： SELIFONOV SERGEY A , STEMMER WILLEM P C , GUSTAFSSON CLAES , TOBIN MATTHEW , DEL CARDAYRE STEPHEN , PATTEN PHILLIP A , MINSHULL JEREMY
IPC分类号： C12N15/09 , C12M1/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N7/00 , C12N15/10 , C12N15/11 , C12P21/02 , C12Q1/68 , G06F17/30 , G06F17/50 , G06F19/00
CPC分类号： C12N15/11 , B01J2219/00689 , C12N15/1031
摘要： "In silico" nucleic acid recombination methods, related integrated systems utilizing genetic operators and libraries made by in silico shuffling methods are provided. An evolutionary process which includes an information manipulation step (application of a genetic algorithm to a character string representing a biopolymer such as a nucleic acid or protein), to create a set of defined information elements (e.g., character strings) which serve as templates for synthesizing physical nucleic acids. The information elements can be placed into a database or otherwise manipulated in silico, e.g., by the recursive application of a GA to the sequences which are produced. Corresponding physical nucleic acids can be subjected to recombination/selection or other diversity generating procedures, with the nucleic acids being deconvoluted (e.g., sequenced or otherwise analyzed) and the overall process repeated, as appropriate, to achieve a desired nucleic acid.
摘要翻译：提供了“计算机”核酸重组方法，利用遗传算子和利用计算机混洗方法制作的图书馆的相关综合系统。包括信息处理步骤（遗传算法应用于表示生物聚合物例如核酸或蛋白质的字符串）的进化过程，以创建一组定义的信息元素（例如，字符串），其用作模板合成物理核酸。信息元素可以被放置在数据库中或者以其它方式被操纵，例如通过将GA递归地应用于所产生的序列。相应的物理核酸可以进行重组/选择或其他多样性生成程序，核酸被解卷积（例如，测序或以其它方式分析），并且适当地重复整个过程以实现所需的核酸。

26. 发明申请

WO2009148616A3 SYSTEMS AND METHODS FOR DETERMINING PROPERTIES THAT AFFECT AN EXPRESSION PROPERTY VALUE OF POLYNUCLEOTIDES IN AN EXPRESSION SYSTEM 审中-公开
标题翻译：用于确定在表达系统中影响多核苷酸的表达性质的性质的系统和方法
公开(公告)号：WO2009148616A3
公开(公告)日：2010-05-27
申请号：PCT/US2009003412
申请日：2009-06-05
申请人： DNA 2 0 INC , WELCH MARK , GUSTAFSSON CLAES
发明人： WELCH MARK , GUSTAFSSON CLAES
IPC分类号： G01N33/48 , C12Q1/68
CPC分类号： C12N15/67 , G06F19/20 , G06F19/22
摘要： A method of designing a polynucleotide sequence encoding a polypeptide sequence of a predetermined polypeptide is provided. A frequency lookup table corresponding to an expression system is obtained. The table comprises a plurality of sequence elements and a plurality of frequency ranges, each frequency range for a corresponding sequence element. Each frequency range is a range of frequencies with which a corresponding sequence element can occur in a polynucleotide. The polynucleotide sequence is defined using the frequency lookup table by determining, for each respective sequence element in the frequency lookup table, whether the respective sequence element encodes a portion of the polypeptide sequence. When the respective sequence element encodes a portion of the polypeptide sequence, the sequence element is incorporated into the polynucleotide at a frequency of occurrence that is within the frequency range specified for the respective sequence element in the lookup table.
摘要翻译：提供了设计编码预定多肽的多肽序列的多核苷酸序列的方法。获得与表达式系统对应的频率查找表。该表包括多个序列元素和多个频率范围，每个频率范围用于相应的序列元素。每个频率范围是在多核苷酸中可以发生相应序列元件的频率范围。使用频率查找表来定义多核苷酸序列，对于频率查找表中的每个相应序列元件，确定各个序列元件是否编码多肽序列的一部分。当各个序列元件编码多肽序列的一部分时，序列元件以在查找表中对于相应序列元件指定的频率范围内的发生频率被并入多核苷酸。

27. 发明申请

WO2008127213A3 METHODS, SYSTEMS, AND SOFTWARE FOR REGULATED OLIGONUCLEOTIDE-MEDIATED RECOMBINATION 审中-公开
标题翻译：用于调节寡核苷酸介导重组的方法，系统和软件
公开(公告)号：WO2008127213A3
公开(公告)日：2008-12-11
申请号：PCT/US0214866
申请日：2002-05-09
申请人： MAXYGEN INC , MINSHULL JEREMY , GUSTAFSSON CLAES , GOVINDARAJAN SRIDAR , ROY AJOY
发明人： MINSHULL JEREMY , GUSTAFSSON CLAES , GOVINDARAJAN SRIDAR , ROY AJOY
IPC分类号： C12Q1/68
CPC分类号： G06F19/22 , B01J2219/007 , B01J2219/00722 , C12N15/1089 , C12Q1/6806 , C12Q2531/113
摘要： The present invention provides novel in silico recombination techniques in which all or part of a nucleic acid recombination procedure is performed or modeled in a digital system. In particular, this invention relates to methods of designing oligonucleotides for regulated recombination that approximates linkage characteristics obtained from fragmentation-based recombination techniques, such as family-based recombination. The methods of this invention include adjusting overlap regions in pairs of overlapping oligonucleotide character strings to bias recombination towards a desired genetic linkage. This invention also provides systems, computer program products, and kits for practicing the methods of the invention.
摘要翻译：本发明提供了新的计算机重组技术，其中在数字系统中执行或建模全部或部分核酸重组程序。特别地，本发明涉及设计用于调节重组的寡核苷酸的方法，其近似由基于片段的重组技术获得的连锁特征，例如基于家族的重组。本发明的方法包括调整重叠的寡核苷酸字符串对中的重叠区域以将重组偏向期望的遗传连锁。本发明还提供了用于实施本发明的方法的系统，计算机程序产品和套件。

28. 发明申请

WO2007022260A3 SYSTEMS AND METHODS FOR DESIGNING AND ORDERING POLYNUCLEOTIDES 审中-公开
标题翻译：用于设计和订购多核苷酸的系统和方法
公开(公告)号：WO2007022260A3
公开(公告)日：2008-08-28
申请号：PCT/US2006031976
申请日：2006-08-15
申请人： DNA 2 0 INC , GUSTAFSSON CLAES , GOVINDARAJAN SRIDHAR , NESS JON E , VILLALOBOS ALAN MARCO , MINSHULL JEREMY
发明人： GUSTAFSSON CLAES , GOVINDARAJAN SRIDHAR , NESS JON E , VILLALOBOS ALAN MARCO , MINSHULL JEREMY
IPC分类号： G06F7/00
CPC分类号： G06F19/26 , G06F19/18 , G06F19/20
摘要： Computer systems, computer program products and methods for designing oligonucleotides are provided. A set of sequence elements is defined. Each sequence element represents an amino acid sequence segment or a nucleic acid sequence segment. The set of sequence elements collectively represent a design nucleic acid sequence. The set of sequence elements are displayed as a plurality of icons in a linear or a near linear arrangement such that each respective icon in the plurality of icons uniquely represents a corresponding sequence element in the set of sequence elements. In this representation, neighboring icons in the plurality of icons represent neighboring sequence elements in the set of sequence elements. Each respective icon in the plurality of icons depicts a directional property for the corresponding sequence element in the set of sequence elements. An oligonucleotide selection module is used to identify oligonucleotides in the design nucleic acid sequence.
摘要翻译：提供计算机系统，计算机程序产品和设计寡核苷酸的方法。定义了一组序列元素。每个序列元件代表氨基酸序列片段或核酸序列片段。序列元件的集合统称为设计核酸序列。序列元素的集合被显示为线性或近似线性布置的多个图标，使得多个图标中的每个相应图标唯一地表示序列元素集合中的相应序列元素。在该表示中，多个图标中的相邻图标表示该组序列元素中的相邻序列元素。多个图标中的每个相应图标描绘了该组序列元素中相应的序列元素的方向属性。寡核苷酸选择模块用于鉴定设计核酸序列中的寡核苷酸。

29. 发明申请

WO2005013090A3 SYSTEMS AND METHODS FOR BIOPOLYMER ENGINEERING 审中-公开
标题翻译：生物聚合物工程系统与方法
公开(公告)号：WO2005013090A3
公开(公告)日：2006-04-13
申请号：PCT/US2004024752
申请日：2004-07-30
申请人： DNA TWOPOINTO INC , GUSTAFSSON CLAES , GOVINDARAJAN SRIDHAR , MINSHULL JEREMY STEPHEN
发明人： GUSTAFSSON CLAES , GOVINDARAJAN SRIDHAR , MINSHULL JEREMY STEPHEN
IPC分类号： C07K16/10 , C07K16/46 , G01N20060101 , G06F20060101 , G06F19/16 , G06F19/18 , G06F19/24 , G01N33/00 , G01N33/48
CPC分类号： G06F19/18 , C07K16/1027 , C07K16/461 , C07K2317/76 , G06F19/16 , G06F19/24
摘要： Methods, computer systems, and computer program products for biopolymer engineering. A variant set for a biopolymer of interest is constructed by identifying, using a plurality of rules, a plurality of positions in the biopolymer of interest and, for each respective position in the plurality of positions, substitutions for the respective position. The plurality of positions and the substitutions for each respective position in the plurality of positions collectively define a biopolymer sequence space. A variant set comprising a plurality of variants of the biopolymer of interest is selected. A property of all or a portion of the variants in the variant set is measured. A sequence-activity relationship is modeled between (i) one or more substitutions at one or more positions of the biopolymer of interest represented by the variant set and (ii) the property measured for all or the portion of the variants in the variant set. The variant set is redefined to comprise variants that include substitutions in the plurality of positions that are selected based on a function of the sequence-activity relationship.
摘要翻译：方法，计算机系统和生物聚合物工程的计算机程序产品。通过使用多个规则来识别感兴趣的生物聚合物中的多个位置，并且对于多个位置中的每个相应位置，通过识别相应位置的替换来构建用于感兴趣的生物聚合物的变体。多个位置中的各个位置的多个位置和替换共同限定生物聚合物序列空间。选择包含感兴趣的生物聚合物的多个变体的变体组。测量变体集中全部或部分变体的属性。序列活性关系在（i）由变体组表示的感兴趣的生物聚合物的一个或多个位置处的一个或多个取代和（ii）针对变体集中的全部或部分变体测量的性质之间建模。变体集被重新定义为包括在基于序列活动关系的函数选择的多个位置中的替换的变体。

30. 发明申请

WO2004029197A8 CO-STIMULATORY MOLECULES 审中-公开
标题翻译： CO刺激分子
公开(公告)号：WO2004029197A8
公开(公告)日：2005-07-21
申请号：PCT/US0219898
申请日：2002-06-21
申请人： MAXYGEN INC , PUNNONEN JUHA , LAZETIC ALEXANDRA L L , LEONG STEVEN R , CHANG JEAN CHIA-CHUN , APT DORIS , GUSTAFSSON CLAES
发明人： PUNNONEN JUHA , LAZETIC ALEXANDRA L L , LEONG STEVEN R , CHANG JEAN CHIA-CHUN , APT DORIS , GUSTAFSSON CLAES
IPC分类号： A61K38/00 , A61K38/17 , C07K14/705 , C12N15/12 , C12Q1/68
CPC分类号： C07K14/70503 , A61K38/00 , C07K14/70532
摘要： The invention provides polynucleotides and polypeptides encoded therefrom having advantageous properties, including an ability of the polypeptides to preferentially bind a human CD28 or CTLA-4 receptor at a level greater or less than the ability of human B7-1 to bind human CD28 or CTLA-4, or to induce or inhibit altered level of T cell proliferation response greater compared to that generated by human B7-1. The polypeptides and polynulceotides of the invention are useful in therapeutic and prophylactic treatment methods, gene therapy applications, and vaccines.
摘要翻译：本发明提供了由其编码的多核苷酸和多肽，其具有有利的性质，包括多肽以大于或小于人B7-1结合人CD28或CTLA-4受体的能力优先结合人CD28或CTLA-4受体的能力， 4，或诱导或抑制T细胞增殖反应的改变水平与人B7-1产生的相比更大。本发明的多肽和多核苷酸可用于治疗和预防性治疗方法，基因治疗应用和疫苗。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式