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11. 发明申请

WO2012161982A1 CHLOROPLAST TRANSIT PEPTIDES AND METHODS OF THEIR USE 审中-公开
标题翻译：氯霉素转运肽及其使用方法
公开(公告)号：WO2012161982A1
公开(公告)日：2012-11-29
申请号：PCT/US2012/037360
申请日：2012-05-10
申请人： E. I. DUPONT DE NEMOURS & COMPANY , BOURETT, Timothy M. , CHUN, Gary C. , LU, Albert L.
发明人： BOURETT, Timothy M. , LU, Albert L.
IPC分类号： C07K14/415 , C12N15/82 , A01H5/00
CPC分类号： C07K14/415 , C12N15/8221
摘要： Methods and compositions are provided for targeting a polypeptide of interest to a chloroplast. Recombinant polynucleotides comprising a nucleotide sequence encoding a chimeric chloroplast transit peptide (CTP) operably linked to a heterologous polynucleotide of interest are provided. In specific embodiments, the chimeric CTP comprises an N-terminal domain operably linked to a central domain operably linked to a C-terminal domain of a CTP to form a chimeric chloroplast transit peptide having CTP activity. Recombinant polypeptides encoding the same, as well as, cells, plant cells, plants and seeds are further provided which comprise the recombinant polynucleotides. Methods of use of the various sequences are also provided.
摘要翻译：提供了将目的多肽靶向叶绿体的方法和组合物。提供了包含编码与目的异源多核苷酸有效连接的嵌合叶绿体转运肽（CTP）的核苷酸序列的重组多核苷酸。在具体实施方案中，嵌合CTP包含可操作地连接到可操作地连接到CTP的C-末端结构域的中心结构域的N-末端结构域，以形成具有CTP活性的嵌合叶绿体转运肽。还提供了编码其的重组多肽，以及细胞，植物细胞，植物和种子，其包含重组多核苷酸。还提供了使用各种序列的方法。

12. 发明申请

WO2011075593A1 MAIZE EVENT DP-040416-8 AND METHODS FOR DETECTION THEREOF 审中-公开
标题翻译：主要事项DP-040416-8及其检测方法
公开(公告)号：WO2011075593A1
公开(公告)日：2011-06-23
申请号：PCT/US2010/060841
申请日：2010-12-16
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DUPONT DE NEMOURS & COMPANY , DIEHN, Scott , LU, Albert L. , NOWATZKI, Timothy M. , NUBEL, Douglas Stuart , REGISTER, III, James Calvin , SCELONGE, Christopher Jay , VILLANELO, M. Alejandra Pascual , YOUNG, Gregory James , YOUNG, Joshua K. , ZHONG, Cathy Xiaoyan
发明人： DIEHN, Scott , LU, Albert L. , NOWATZKI, Timothy M. , NUBEL, Douglas Stuart , REGISTER, III, James Calvin , SCELONGE, Christopher Jay , VILLANELO, M. Alejandra Pascual , YOUNG, Gregory James , YOUNG, Joshua K. , ZHONG, Cathy Xiaoyan
IPC分类号： C12N15/82 , A01H1/02 , C07K14/325 , A01H5/00
CPC分类号： C12N15/8286 , A01H1/02 , Y02A40/162 , Y10T436/143333
摘要： The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-040416-8 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.
摘要翻译：本发明提供了涉及转基因抗虫玉米植物的DNA组合物。还提供了基于插入到玉米基因组中的重组构建体的DNA序列和插入位点侧翼的DNA序列来检测玉米DP-040416-8事件的存在的测定法。提供了用于进行测定的试剂盒和条件。

13. 发明申请

WO2009091862A3 COMPOSITIONS AND METHODS FOR THE SUPPRESSION OF TARGET POLYNUCLEOTIDES FROM LEPIDOPTERA 审中-公开
公开(公告)号：WO2009091862A3
公开(公告)日：2009-07-23
申请号：PCT/US2009/031055
申请日：2009-01-15
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E.I. DUPONT DE NEMOURS & COMPANY , HERRMANN, Rafael , LASSNER, Michael , LU, Albert L. , NELSON, Mark , PRESNAIL, James K. , RICE, Janet A.
发明人： HERRMANN, Rafael , LASSNER, Michael , LU, Albert L. , NELSON, Mark , PRESNAIL, James K. , RICE, Janet A.
IPC分类号： C12N15/82
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Lepidoptera order, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides target polynucleotides encoding polypeptides from specific protein families and various target polynucleotides set forth in SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Spodoptera frugiperda. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.

14. 发明申请

WO2007084741A3 METHOD FOR ASSESSMENT OF UNCERTAINTY AND RISK 审中-公开
标题翻译：评估不确定性和风险的方法
公开(公告)号：WO2007084741A3
公开(公告)日：2009-01-15
申请号：PCT/US2007001588
申请日：2007-01-19
申请人： SCHLUMBERGER SERVICES PETROL , SCHLUMBERGER CA LTD , LOGINED BV , PRAD RES AND DEV LTD A BRITISH , TABANOU ERIC , PALKOWSKY DOUGLAS A , LU ALBERT , BRADY JAMES P , NEISCH DANIEL A
发明人： TABANOU ERIC , PALKOWSKY DOUGLAS A , LU ALBERT , BRADY JAMES P , NEISCH DANIEL A
IPC分类号： G06Q40/00
CPC分类号： G06Q10/06
摘要： A method is disclosed for assessing uncertainty comprising: creating a catalog for uncertainty areas; capturing quantitative and qualitative uncertainty data; establishing dependencies between uncertainties; associating risks to uncertainties; associating action plans and tasks to risks; creating a realization tree from uncertainty ranges; and tracking changes to uncertainties and realizations over time.
摘要翻译：公开了一种用于评估不确定性的方法，包括：为不确定区域创建目录; 捕获定量和定性不确定性数据; 建立不确定性之间的依赖关系; 将风险与不确定因素相关联; 将行动计划和任务与风险联系起来; 从不确定性范围创建一个实现树; 并随着时间的推移跟踪不确定性和实现的变化。

15. 发明申请

WO2007024866A2 METHODS AND COMPOSITIONS FOR THE EXPRESSION OF A POLYNUCLEOTIDE OF INTEREST 审中-公开
标题翻译：用于表达多核苷酸的方法和组合物
公开(公告)号：WO2007024866A2
公开(公告)日：2007-03-01
申请号：PCT/US2006032792
申请日：2006-08-22
申请人： PIONEER HI BRED INT , DU PONT , MCCUTCHEN BILLY FRED , HAZEL CHRISTINE B , LIU DONGLONG , LU ALBERT L , MEHRE WAYNE J , OLSON PAUL D , WONG JAMES F H
发明人： MCCUTCHEN BILLY FRED , HAZEL CHRISTINE B , LIU DONGLONG , LU ALBERT L , MEHRE WAYNE J , OLSON PAUL D , WONG JAMES F H
IPC分类号： A63B20060101 , A63B69/00 , A63B69/36 , A63B71/06
CPC分类号： C12N15/8274 , C12N9/1092 , C12N9/88 , C12N15/8205 , C12N15/8209 , C12N15/8216 , C12N15/8271 , C12N15/8275 , C12N15/8278 , C12N15/8286 , Y02A40/162
摘要： Methods and compositions for expressing a polynucleotide of interest are provided. Compositions comprise an enhancer domain set forth in SEQ ID NO: 1, 10, 15, or 16 and active variants and fragments thereof. Further provided are DNA constructs comprising at least one transcriptional enhancer sequence comprising the nucleotide sequence set forth in SEQ ID NO:1, 10, 15, or 16 or an active variant or fragment thereof, operably linked to a heterologous promoter. Such chimeric transcription regulatory regions can be operably linked any polynucleotide of interest. Further provided are cells, plants, plant parts, and germplasm comprising the DNA construct. Methods of using the chimeric transcriptional regulatory region are also provided. In specific embodiments, methods of expressing a polynucleotide of interest, including for example, sequences that confer tolerance to herbicides, and methods to select a cell having the DNA construct are provided.
摘要翻译：提供了表达感兴趣的多核苷酸的方法和组合物。组合物包含SEQ ID NO：10,15或16所示的增强子结构域及其活性变体和片段。还提供了包含至少一个转录增强子序列的DNA构建体，所述转录增强子序列包含可操作地连接到异源启动子的SEQ ID NO：1,0,15或16所示的核苷酸序列或其活性变体或片段。这样的嵌合转录调控区域可以可操作地连接任何感兴趣的多核苷酸。还提供了包含DNA构建体的细胞，植物，植物部分和种质。还提供了使用嵌合转录调节区的方法。在具体实施方案中，提供了表达目标多核苷酸的方法，包括例如赋予除草剂耐受性的序列，以及选择具有DNA构建体的细胞的方法。

16. 发明申请

WO2006133182A3 INSECT-SPECIFIC PROTEASE RECOGNITION SEQUENCES 审中-公开
标题翻译： INSECT-SPECIFIC PROTEASE识别序列
公开(公告)号：WO2006133182A3
公开(公告)日：2007-02-08
申请号：PCT/US2006021902
申请日：2006-06-06
申请人： PIONEER HI BRED INT , DU PONT , ABAD ANDRE R , CHUN GARY C , FLANNAGAN RONALD D , HERRMANN RAFAEL , LU ALBERT L , MCCUTCHEN BILLY F , RICE JANET A , SCHEPERS ERIC J
发明人： ABAD ANDRE R , CHUN GARY C , FLANNAGAN RONALD D , HERRMANN RAFAEL , LU ALBERT L , MCCUTCHEN BILLY F , RICE JANET A , SCHEPERS ERIC J
IPC分类号： C07K14/325 , A01N63/02 , C12N9/64 , C12N15/82
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/325 , C12N9/6472 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. Nucleic acid molecules encoding insect protoxins or insect toxins modified to comprise at least one proteolytic activation site that is sensitive to an insect gut protease are provided. Cleavage of a modified insect protoxin at the proteolytic activation site by an insect gut protease produces an active insect toxin in the gut of the insect pest. Cleavage of a modified insect toxin of the invention at a proteolytic activation site results in the production of an active insect toxin in the insect gut that displays improved pesticidal activity relative to the insect toxin that lacks the proteolytic activation site. Methods of using the modified insect protoxin and modified insect toxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin and modified insect toxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds. Insect gut proteases and the nucleic acid molecules that encode them are also disclosed herein.
摘要翻译：提供了保护植物免受害虫害虫的组合物和方法。提供编码昆虫原毒素或昆虫毒素的核酸分子，其被修饰为包含至少一个对昆虫肠道蛋白酶敏感的蛋白水解活化位点。通过昆虫肠蛋白酶在蛋白水解活化位点切割修饰的昆虫原毒素在害虫的肠道中产生活性昆虫毒素。在蛋白水解活化位点切割本发明的修饰的昆虫毒素导致在昆虫肠中产生活性昆虫毒素，其相对于缺乏蛋白水解活化位点的昆虫毒素显示出改善的杀虫活性。提供了使用修饰的昆虫原毒素和修饰的昆虫毒素核酸序列及其编码的多肽以保护植物免受害虫的方法。本发明的具体实施方案还提供改性的昆虫原毒素和修饰的昆虫毒素组合物和制剂，表达盒，以及转化的植物，植物细胞和种子。昆虫肠道蛋白酶和编码它们的核酸分子也在本文中公开。

17. 发明申请

WO2006055487A3 MAIZE CR1BIO GENE PROMOTER AND ITS USE TO DIRECT ROOT-PREFERRED TRANSGENE EXPRESSION IN PLANTS 审中-公开
标题翻译：玉米CR1BIO基因启动子及其在植物中直接优先转录表达的用途
公开(公告)号：WO2006055487A3
公开(公告)日：2006-09-21
申请号：PCT/US2005041186
申请日：2005-11-15
申请人： PIONEER HI BRED INT , DU PONT , DIEHN SCOTT , LU ALBERT L , SIMS LYNNE E , WARD KIM R
发明人： DIEHN SCOTT , LU ALBERT L , SIMS LYNNE E , WARD KIM R
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12N15/8234 , C12N15/8227
摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a root-preferred promoter for the gene encoding Cr1Bio. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stabling incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
摘要翻译：本发明提供了用于调节植物中异源核苷酸序列表达的组合物和方法。组合物包括编码Cr1Bio的基因的根优选启动子的新型核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括使与本发明的根优选启动子有效连接的核苷酸序列与植物细胞的基因组结合并再生表达该核苷酸序列的稳定转化的植物。

18. 发明申请

WO2005118628A3 NUCLEIC ACID MOLECULES ENCODING PLANT CYCLOTIDE POLYPEPTIDES AND METHODS OF USE 审中-公开
标题翻译：编码植物循环多聚核苷酸的核酸分子及其使用方法
公开(公告)号：WO2005118628A3
公开(公告)日：2006-05-04
申请号：PCT/US2005018433
申请日：2005-05-25
申请人： DU PONT , HERRMANN RAFAEL , LU ALBERT LAURENCE , MCCUTCHEN BILLY F , PRESNAIL JAMES K , WAINE CLEMENT W K
发明人： HERRMANN RAFAEL , LU ALBERT LAURENCE , MCCUTCHEN BILLY F , PRESNAIL JAMES K , WAINE CLEMENT W K
IPC分类号： C07K14/415 , A01H1/00 , A61K38/16 , C07H21/04 , C07K7/64 , C07K14/00 , C12N5/04 , C12N15/82
CPC分类号： C12N15/8285 , C07K14/415 , C12N15/8282 , Y02A40/164
摘要： The present invention relates to isolated nucleic acids encoding plant cyclotides. The invention also relates to the construction of a chimeric gene encoding all or a portion of the plant cyclotides, in sense or antisense orientation, wherein expression of the chimeric gene results in the production of altered levels of plant cyclotides in a transformed host cell.
摘要翻译：本发明涉及编码植物环肽的分离的核酸。本发明还涉及在正义或反义方向上构建编码全部或部分植物环肽的嵌合基因，其中嵌合基因的表达导致在转化的宿主细胞中产生改变水平的植物环磷酰胺。

19. 发明申请

WO2005063996A2 PLANT ACTIVATION OF INSECT TOXIN 审中-公开
标题翻译：昆虫毒素的植物激活
公开(公告)号：WO2005063996A2
公开(公告)日：2005-07-14
申请号：PCT/US2004/042736
申请日：2004-12-20
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E.I. DU PONT DE NEMOURS AND COMPANY , ABAD, André , FLANNAGAN, Ronald, D. , HERRMANN, Rafael , LU, Albert , MCCUTCHEN, Billy, F. , PRESNAIL, James, K. , RICE, Janet, A. , WONG, James, F. , YU, Cao-Guo
发明人： ABAD, André , FLANNAGAN, Ronald, D. , HERRMANN, Rafael , LU, Albert , MCCUTCHEN, Billy, F. , PRESNAIL, James, K. , RICE, Janet, A. , WONG, James, F. , YU, Cao-Guo
IPC分类号： C12N15/82
CPC分类号： C12N15/8286 , C12N9/641 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.
摘要翻译：提供了用于保护植物免受害虫侵害的组合物和方法。具体而言，提供了编码经修饰以包含至少一个对植物蛋白酶或昆虫肠蛋白酶敏感的蛋白水解活化位点的昆虫原毒素的核酸序列。在蛋白水解活化位点被蛋白酶切割修饰的原毒素产生活性昆虫毒素。提供了使用修饰的昆虫原毒素核酸序列和它们编码的多肽来保护植物免受害虫的方法。本发明的特定实施方案进一步提供了修饰的昆虫原毒素组合物和制剂，表达盒和转化的植物，植物细胞和种子。

20. 发明申请

WO02086072A8 ANTIMICROBIAL POLYPEPTIDES AND THEIR USES 审中-公开
标题翻译：抗微生物多肽及其用途
公开(公告)号：WO02086072A8
公开(公告)日：2002-12-12
申请号：PCT/US0212511
申请日：2002-04-19
申请人： PIONEER HI BRED INT , ALTIER DANIEL J , HERRMANN RAFAEL , LU ALBERT L , MCCUTCHEN BILLY F , PRESNAIL JAMES K , WEAVER JANINE L , WONG JAMES F H , DU PONT
发明人： ALTIER DANIEL J , HERRMANN RAFAEL , LU ALBERT L , MCCUTCHEN BILLY F , PRESNAIL JAMES K , WEAVER JANINE L , WONG JAMES F H
IPC分类号： C07K14/435 , C12N1/15 , C12N1/19 , C12N1/21 , C12N7/01 , C12N15/12 , C12N15/82 , C12N
CPC分类号： C12N15/8282 , C07K14/43563 , Y10S530/858
摘要： The methods and compositions of the present invention find use in impacting microbial pathogens and in enhancing disease resistance to pathogens, particularly by plants. The compositions of the invention include polypeptides that possess antimicrobial properties, particularly fungicidal properties, and the encoding nucleic acid molecules. The polypeptides of the invention are isolated from the hemolymph and fat bodies of insect larvae induced by injection of plant pathogenic fungi. Further provided are plant cells, plants, an seed thereof, transformed with the nucleic acid mole cules of the invention so as to confer disease resistance on the plant.
摘要翻译：本发明的方法和组合物用于影响微生物病原体并增强对病原体的抗病性，特别是植物的抗病性。本发明的组合物包括具有抗微生物特性，特别是杀真菌性质和编码核酸分子的多肽。通过注射植物致病真菌诱导的昆虫幼虫的血淋巴和脂肪体分离本发明的多肽。进一步提供了用本发明的核酸摩尔转化的植物细胞，植物及其种子，从而赋予植物抗病性。

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