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1. 发明申请

WO2007087567A8 ANTIFUNGAL POLYPEPTIDES 审中-公开
标题翻译：抗菌多糖
公开(公告)号：WO2007087567A8
公开(公告)日：2009-07-30
申请号：PCT/US2007060982
申请日：2007-01-24
申请人： PIONEER HI BRED INT , DU PONT , UNIV CALIFORNIA , ALTIER DANIEL J , GILLIAM JACOB T , HUNTER-CEVERA JENNIE , PRESNAIL JAMES K , SCHEPERS ERIC J , SIMMONS CARL R , TOROK TAMAS , YALPANI NASSER
发明人： ALTIER DANIEL J , GILLIAM JACOB T , HUNTER-CEVERA JENNIE , PRESNAIL JAMES K , SCHEPERS ERIC J , SIMMONS CARL R , TOROK TAMAS , YALPANI NASSER , ELLANSKAYA I A
IPC分类号： C12N15/82 , A01H5/00 , C12Q1/68 , C12R1/77
CPC分类号： C12N15/8282 , A01N37/46 , A01N63/04 , C07K14/37
摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include an amino acid sequence, and variants and fragments thereof, for an antipathogenic polypeptide that was isolated from a fungal fermentation broth. Nucleic acid molecules that encode the antipathogenic polypeptides of the invention, and antipathogenic domains thereof, are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention are also disclosed.
摘要翻译：提供了用于保护植物免受病原体，特别是真菌病原体的组合物和方法。组合物包括从真菌发酵液中分离的抗病原体多肽的氨基酸序列及其变体和片段。还提供了编码本发明的抗病原体多肽的核酸分子及其抗病原结构域。还提供使用本文公开的核苷酸序列在植物中诱导病原体抗性的方法。该方法包括向植物中引入包含可操作地连接到编码本发明的抗病原体多肽的核苷酸序列的启动子的表达盒。还提供包含抗病原体多肽或包含本发明的核酸与载体组合的转化微生物的组合物，以及使用这些组合物保护植物免受病原体的方法。还公开了包含编码本发明的抗病原体多肽的核苷酸序列的转化植物，植物细胞，种子和微生物。

2. 发明申请

WO1999004013A2 THE INDUCTION OF STRESS-RELATED FACTORS IN PLANTS 审中-公开
标题翻译：植物中胁迫相关因子的诱导
公开(公告)号：WO1999004013A2
公开(公告)日：1999-01-28
申请号：PCT/US1998014861
申请日：1998-07-17
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , ALTIER, Daniel, J. , BIDNEY, Dennis, L. , COUGHLAN, Sean, J. , FALAK, Igor , YALPANI, Nasser , LU, Guihau , MANCL, Mark, K. , NAZARIAN, Katherine, A., P. , SCELONGE, Christopher, J.
发明人： PIONEER HI-BRED INTERNATIONAL, INC.
IPC分类号： C12N15/53
CPC分类号： C12N9/0008 , C12N9/0006 , C12N15/8271 , C12N15/8282 , C12Q1/26
摘要： The present invention relates to a method of producing resistance to stress in a variety of plants by the expression of a hydrogen peroxide or reactive oxygen species producing enzyme such as, but not limited to, oxalate oxidase. In addition, the present invention relates to a method of producing a stress resistant sunflower plant by transforming a sunflower plant with an oxalate oxidase gene or other hydrogen peroxide/reactive oxygen species producing genes that upon expression of the gene produce an enzyme capable of generating hydrogen peroxide or reactive oxygen species from endogeously available substrate. Alternatively, the plant could be transformed with the hydrogen peroxide or reactive oxygen species producing gene and a gene for the necessary substrate or a gene which would modify a pathway in the plant resulting in formation or increase in substrate. The production of hydrogen peroxide or a reactive oxygen species triggers the expression of hydrogen peroxide/reactive oxygen species-responsive genes resulting ina pre-challenge accumulation of pathogenesis or stress-related factors such as salicylic acid and PR protein. Thus the plant is rendered resistant to a variety of stresses by, for example, inhibiting the establishment of a pathogen resulting in a disease resistance phenotype.
摘要翻译：本发明涉及通过表达产生酶如草酸盐的过氧化氢或活性氧种类在各种植物中产生胁迫抗性的方法但不仅如此。另外，本发明涉及使用草酸氧化酶基因或其他产生过氧化氢的基因/物种通过向日葵的转化来制造对抗胁迫的向日葵的方法活性氧在表达后产生能够从可利用的内源性底物产生过氧化氢或活性氧物种的酶。根据另一个实施方案，所述植物可以通过产生过氧化氢的基因或活性氧的种类以及通过作用于必需底物的基因或通过改变植物过程的基因来转化。从而导致所述衬底的形成或扩大。过氧化氢或活性氧的产生触发了对这些物质表达敏感的基因的表达，导致发病前或应激相关因素的预先积聚，例如比水杨酸和PR蛋白（与发病有关）。因此，通过例如抑制产生疾病抗性表型的病原体的建立使植物抵抗多种胁迫。

3. 发明申请

WO2012021500A2 ANTIPATHOGENIC PROTEINS AND METHODS OF USE 审中-公开
标题翻译：抗病原蛋白和使用方法
公开(公告)号：WO2012021500A2
公开(公告)日：2012-02-16
申请号：PCT/US2011/047056
申请日：2011-08-09
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DUPONT DE NEMOURS & COMPANY , ALTIER, Daniel J. , GILLIAM, Jacob T. , SCHEPERS, Eric J. , YALPANI, Nasser
发明人： ALTIER, Daniel J. , GILLIAM, Jacob T. , SCHEPERS, Eric J. , YALPANI, Nasser
IPC分类号： C12N15/31 , C07K14/37 , A01H5/00 , C12N15/82 , A01N63/04
CPC分类号： C07K14/37 , A01N63/04 , C12N15/8282
摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include amino acid sequences, and variants and fragments thereof, for family members of a novel family of antipathogenic polypeptides. Polynucleotides that encode the antipathogenic polypeptides are also provided. A method for inducing pathogen resistance in a plant using the polynucleotides disclosed herein is further provided. Compositions comprising an antipathogenic polypeptide or a microorganism comprising an antipathogenic polynucleotide of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Plants, plant cells, seeds, and microorganisms comprising an antipathogenic polnucleotide or polypeptide of the invention are also disclosed.
摘要翻译：提供了用于保护植物免受病原体，特别是真菌病原体的组合物和方法。组合物包括氨基酸序列及其变体和片段，用于新型抗病原体多肽家族的家族成员。还提供了编码抗病原多肽的多核苷酸。还提供了使用本文公开的多核苷酸在植物中诱导病原体抗性的方法。还提供了包含抗病原体多肽或包含本发明的抗病原体多核苷酸与载体组合的组合物，以及使用这些组合物保护植物免受病原体的方法。还公开了包含本发明的抗病原体核苷酸或多肽的植物，植物细胞，种子和微生物。

4. 发明申请

WO2005030967A3 CROP PLANT CYSTATIN PROTEINASE INHIBITORS AND METHODS OF USE 审中-公开
公开(公告)号：WO2005030967A3
公开(公告)日：2005-04-07
申请号：PCT/US2004/030894
申请日：2004-09-22
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , ALTIER, Daniel, J. , BAO, Zhongmeng , LU, Guihua , NAVARRO ACEVEDO, Pedro, A. , SEWALT, Vincent, J. H. , SIMMONS, Carl, R. , YALPANI, Nasser
发明人： ALTIER, Daniel, J. , BAO, Zhongmeng , LU, Guihua , NAVARRO ACEVEDO, Pedro, A. , SEWALT, Vincent, J. H. , SIMMONS, Carl, R. , YALPANI, Nasser
IPC分类号： C07K14/81
摘要： Methods and compositions for modulating development and defense responses are provided. Nucleotide sequences encoding maize, soybean, wheat and rice cystatin proteins are provided. The sequences can be used in expression cassettes for modulating development, developmental pathways, and defense responses. Transformed plants, plant cells, tissues, and seed are also provided.

5. 发明申请

WO02086072A3 ANTIMICROBIAL POLYPEPTIDES AND THEIR USES 审中-公开
标题翻译：抗微生物多肽及其用途
公开(公告)号：WO02086072A3
公开(公告)日：2003-06-05
申请号：PCT/US0212511
申请日：2002-04-19
申请人： PIONEER HI BRED INT , ALTIER DANIEL J , HERRMANN RAFAEL , LU ALBERT L , MCCUTCHEN BILLY F , PRESNAIL JAMES K , WEAVER JANINE L , WONG JAMES F H , DU PONT
发明人： ALTIER DANIEL J , HERRMANN RAFAEL , LU ALBERT L , MCCUTCHEN BILLY F , PRESNAIL JAMES K , WEAVER JANINE L , WONG JAMES F H
IPC分类号： C07K14/435 , C12N1/15 , C12N1/19 , C12N1/21 , C12N7/01 , C12N15/12 , C12N15/82 , A01H5/00 , C12N5/04 , C12N15/70 , C12N15/74 , C12N15/80 , C12N15/81
CPC分类号： C12N15/8282 , C07K14/43563 , Y10S530/858
摘要： The methods and compositions of the present invention find use in impacting microbial pathogens and in enhancing disease resistance to pathogens, particularly by plants. The compositions of the invention include polypeptides that possess antimicrobial properties, particularly fungicidal properties, and the encoding nucleic acid molecules. The polypeptides of the invention are isolated from the hemolymph and fat bodies of insect larvae induced by injection of plant pathogenic fungi. Further provided are plant cells, plants, an seed thereof, transformed with the nucleic acid mole cules of the invention so as to confer disease resistance on the plant.
摘要翻译：本发明的方法和组合物用于影响微生物病原体并增强对病原体的抗病性，特别是植物的抗病性。本发明的组合物包括具有抗微生物特性，特别是杀真菌性质和编码核酸分子的多肽。通过注射植物致病真菌诱导的昆虫幼虫的血淋巴和脂肪体分离本发明的多肽。进一步提供了用本发明的核酸摩尔转化的植物细胞，植物及其种子，从而赋予植物抗病性。

6. 发明申请

WO2007149657A3 ANTIFUNGAL POLYPEPTIDES 审中-公开
公开(公告)号：WO2007149657A3
公开(公告)日：2007-12-27
申请号：PCT/US2007/068984
申请日：2007-05-15
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E.I. DU PONT DE NEMOURS AND COMPANY , THE REGENTS OF THE UNIVERSITY OF CALIFORNIA , ALTIER, Daniel, J. , CRANE, Virginia, C. , GILLIAM, Jacob, T. , HUNTER-CEVERA, Jennie , PRESNAIL, James, K. , SCHEPERS, Eric, J. , SIMMONS, Carl, R. , TOROK, Tamas , YALPANI, Nasser
发明人： ALTIER, Daniel, J. , CRANE, Virginia, C. , GILLIAM, Jacob, T. , HUNTER-CEVERA, Jennie , PRESNAIL, James, K. , SCHEPERS, Eric, J. , SIMMONS, Carl, R. , TOROK, Tamas , YALPANI, Nasser
IPC分类号： C12N15/82 , C07K14/385
摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from fungal fermentation broths. Nucleic acids that encode the antipathogenic polypeptides are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention are also disclosed.

7. 发明申请

WO2007087567A2 ANTIFUNGAL POLYPEPTIDES 审中-公开
标题翻译：抗菌多糖
公开(公告)号：WO2007087567A2
公开(公告)日：2007-08-02
申请号：PCT/US2007060982
申请日：2007-01-24
申请人： PIONEER HI BRED INT , DU PONT , UNIV CALIFORNIA , ALTIER DANIEL J , GILLIAM JACOB T , HUNTER-CEVERA JENNIE , PRESNAIL JAMES K , SCHEPERS ERIC J , SIMMONS CARL R , TOROK TAMAS , YALPANI NASSER
发明人： ALTIER DANIEL J , GILLIAM JACOB T , HUNTER-CEVERA JENNIE , PRESNAIL JAMES K , SCHEPERS ERIC J , SIMMONS CARL R , TOROK TAMAS , YALPANI NASSER , ELLESKAYA I A
IPC分类号： C12N15/82
CPC分类号： C12N15/8282 , A01N37/46 , A01N63/04 , C07K14/37
摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include an amino acid sequence, and variants and fragments thereof, for an antipathogenic polypeptide that was isolated from a fungal fermentation broth. Nucleic acid molecules that encode the antipathogenic polypeptides of the invention, and antipathogenic domains thereof, are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention are also disclosed.
摘要翻译：提供了用于保护植物免受病原体，特别是真菌病原体的组合物和方法。组合物包括从真菌发酵液中分离的抗病原体多肽的氨基酸序列及其变体和片段。还提供了编码本发明的抗病原体多肽的核酸分子及其抗病原结构域。还提供了使用本文公开的核苷酸序列在植物中诱导病原体抗性的方法。该方法包括向植物中引入包含可操作地连接到编码本发明的抗病原体多肽的核苷酸序列的启动子的表达盒。还提供包含抗病原体多肽或包含本发明的核酸与载体组合的转化微生物的组合物以及使用这些组合物保护植物免受病原体的方法。还公开了包含编码本发明的致病性多肽的核苷酸序列的转化植物，植物细胞，种子和微生物。

8. 发明申请

WO2005030967A2 CROP PLANT CYSTATIN PROTEINASE INHIBITORS AND METHODS OF USE 审中-公开
标题翻译：作物植物细胞色素蛋白酶抑制剂及使用方法
公开(公告)号：WO2005030967A2
公开(公告)日：2005-04-07
申请号：PCT/US2004030894
申请日：2004-09-22
申请人： PIONEER HI BRED INT , ALTIER DANIEL J , BAO ZHONGMENG , LU GUIHUA , NAVARRO ACEVEDO PEDRO A , SEWALT VINCENT J H , SIMMONS CARL R , YALPANI NASSER
发明人： ALTIER DANIEL J , BAO ZHONGMENG , LU GUIHUA , NAVARRO ACEVEDO PEDRO A , SEWALT VINCENT J H , SIMMONS CARL R , YALPANI NASSER
IPC分类号： A01H1/00 , C07H21/04 , C07K14/81 , C12N5/04 , C12N9/99 , C12N15/82
CPC分类号： C07K14/8139 , C12N15/8279
摘要： Methods and compositions for modulating development and defense responses are provided. Nucleotide sequences encoding maize, soybean, wheat and rice cystatin proteins are provided. The sequences can be used in expression cassettes for modulating development, developmental pathways, and defense responses. Transformed plants, plant cells, tissues, and seed are also provided.
摘要翻译：提供了用于调节发育和防御反应的方法和组合物。提供了编码玉米，大豆，小麦和水稻半胱氨酸蛋白酶抑制蛋白的核苷酸序列。这些序列可以用于调控发育，发育途径和防御反应的表达盒中。还提供了转化的植物，植物细胞，组织和种子。

9. 发明申请

WO2002086072A2 ANIMICROBIAL POLYPEPTIDES AND THEIR USES 审中-公开
标题翻译：动物多肽及其用途
公开(公告)号：WO2002086072A2
公开(公告)日：2002-10-31
申请号：PCT/US2002/012511
申请日：2002-04-19
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E.I. DU PONT DE NEMOURS AND COMPANY , ALTIER, Daniel, J. , HERRMANN, Rafael , LU, Albert, L. , McCUTCHEN, Billy, F. , PRESNAIL, James, K. , WEAVER, Janine, L. , WONG, James, F., H.
发明人： ALTIER, Daniel, J. , HERRMANN, Rafael , LU, Albert, L. , McCUTCHEN, Billy, F. , PRESNAIL, James, K. , WEAVER, Janine, L. , WONG, James, F., H.
IPC分类号： C12N
CPC分类号： C12N15/8282 , C07K14/43563 , Y10S530/858
摘要： The methods and compositions of the present invention find use in impacting microbial pathogens and in enhancing disease resistance to pathogens, particularly by plants. The compositions of the invention include polypeptides that possess antimicrobial properties, particularly fungicidal properties, and the encoding nucleic acid molecules. The polypeptides of the invention are isolated from the hemolymph and fat bodies of insect larvae induced by injection of plant pathogenic fungi. Further provided are plant cells, plants, an seed thereof, transformed with the nucleic acid mole cules of the invention so as to confer disease resistance on the plant.
摘要翻译：本发明的方法和组合物用于影响微生物病原体并增强对病原体的抗病性，特别是植物的抗病性。本发明的组合物包括具有抗微生物特性，特别是杀真菌性质和编码核酸分子的多肽。通过注射植物致病真菌诱导的昆虫幼虫的血淋巴和脂肪体分离本发明的多肽。进一步提供了用本发明的核酸摩尔转化的植物细胞，植物及其种子，从而赋予植物抗病性。

10. 发明申请

WO9904013A3 THE INDUCTION OF STRESS-RELATED FACTORS IN PLANTS 审中-公开
标题翻译：植物中应力相关因素的诱导
公开(公告)号：WO9904013A3
公开(公告)日：1999-05-14
申请号：PCT/US9814861
申请日：1998-07-17
申请人： PIONEER HI BRED INT , ALTIER DANIEL J , BIDNEY DENNIS L , COUGHLAN SEAN J , FALAK IGOR , YALPANI NASSER , LU GUIHAU , MANCL MARK K , NAZARIAN KATHERINE A P , SCELONGE CHRISTOPHER J
发明人： ALTIER DANIEL J , BIDNEY DENNIS L , COUGHLAN SEAN J , FALAK IGOR , YALPANI NASSER , LU GUIHAU , MANCL MARK K , NAZARIAN KATHERINE A P , SCELONGE CHRISTOPHER J
IPC分类号： C12N9/02 , C12N9/04 , C12N15/53 , C12N15/82 , C12Q1/26 , A01H5/00 , C12N5/10
CPC分类号： C12N9/0008 , C12N9/0006 , C12N15/8271 , C12N15/8282 , C12Q1/26
摘要： The present invention relates to a method of producing resistance to stress in a variety of plants by the expression of a hydrogen peroxide or reactive oxygen species producing enzyme such as, but not limited to, oxalate oxidase. In addition, the present invention relates to a method of producing a stress resistant sunflower plant by transforming a sunflower plant with an oxalate oxidase gene or other hydrogen peroxide/reactive oxygen species producing genes that upon expression of the gene produce an enzyme capable of generating hydrogen peroxide or reactive oxygen species from endogeously available substrate. Alternatively, the plant could be transformed with the hydrogen peroxide or reactive oxygen species producing gene and a gene for the necessary substrate or a gene which would modify a pathway in the plant resulting in formation or increase in substrate. The production of hydrogen peroxide or a reactive oxygen species triggers the expression of hydrogen peroxide/reactive oxygen species-responsive genes resulting ina pre-challenge accumulation of pathogenesis or stress-related factors such as salicylic acid and PR protein. Thus the plant is rendered resistant to a variety of stresses by, for example, inhibiting the establishment of a pathogen resulting in a disease resistance phenotype.
摘要翻译：本发明涉及通过表达过氧化氢或活性氧产生酶，例如但不限于草酸氧化酶，在各种植物中产生抗应力的方法。此外，本发明涉及一种通过用草酸氧化酶基因或其他过氧化氢/活性氧转化向日葵植物产生抗胁迫向日葵植物的方法，其产生基因表达时基因产生能产生氢的酶过氧化物或来自内在可用底物的活性氧。或者，可以用过氧化氢或活性氧产生基因和必需底物的基因或将修饰植物中途径导致底物形成或增加的基因转化植物。过氧化氢或活性氧的产生触发了过氧化氢/活性氧物质反应基因的表达，导致了攻击前积累的发病机制或应激相关因素如水杨酸和PR蛋白。因此，通过例如抑制导致抗病性表型的病原体的建立，使植物对各种胁迫具有抗性。

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