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1. 发明申请

WO2014116989A1 MAIZE EVENT DP-032218-9 AND METHODS FOR DETECTION THEREOF 审中-公开
标题翻译：主要事项DP-032218-9及其检测方法
公开(公告)号：WO2014116989A1
公开(公告)日：2014-07-31
申请号：PCT/US2014/013007
申请日：2014-01-24
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DUPONT DE NEMOURS & COMPANY , BEATTY, Mary E. H. , BRINK, Kent , CRANE, Virginia C. , DIEHN, Scott , LU, Albert L. , YOUNG, Gregory J.
发明人： BEATTY, Mary E. H. , BRINK, Kent , CRANE, Virginia C. , DIEHN, Scott , LU, Albert L. , YOUNG, Gregory J.
IPC分类号： C12N15/82
CPC分类号： C12Q1/6895 , A01H5/10 , C12N15/8277 , C12N15/8286 , C12Q2600/13 , C12Q2600/158 , Y02A40/162
摘要： The disclosure provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-032218-9 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.
摘要翻译：本公开提供与转基因抗虫玉米植物相关的DNA组合物。还提供了基于插入到玉米基因组中的重组构建体的DNA序列和插入位点侧翼的DNA序列来检测玉米DP-032218-9事件的存在的测定法。提供了用于进行测定的试剂盒和条件。

2. 发明申请

WO2011153418A3 COMPOSITIONS AND METHODS FOR INSECTICIDAL CONTROL OF STINKBUGS 审中-公开
标题翻译： STINKBUGS的杀虫剂控制组合物和方法
公开(公告)号：WO2011153418A3
公开(公告)日：2012-05-31
申请号：PCT/US2011039042
申请日：2011-06-03
申请人： BROGLIE KAREN E , CERF DAVID C , HERRMANN RAFAEL , LU ALBERT L , MCGONIGLE BRIAN , PRESNAIL JAMES K
发明人： BROGLIE KAREN E , CERF DAVID C , HERRMANN RAFAEL , LU ALBERT L , MCGONIGLE BRIAN , PRESNAIL JAMES K
IPC分类号： A01H5/00 , C12N15/31 , C12N15/82
CPC分类号： C12N15/8286 , A01N57/16 , A01N65/00 , A01N65/44 , C12N15/113 , C12N15/8218 , C12N2310/14 , C12N2310/531 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Pentatomidae plant pest or a N. viridula, Acrosternum hilare, Piezodorus guildini, and/or Halymorpha halys plant pest, decrease the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: 1-292 or 302-304 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Pentatomidae. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要翻译：提供了使用沉积元件的方法和组合物，当被害虫摄入时，例如五子科植物害虫或N. viridula，Acrosternum hilare，Piezodorus guildini和/或Halymorpha halys植物害虫，降低靶的表达序列在害虫中。在具体实施方案中，靶序列的表达降低控制有害生物，因此方法和组合物能够限制对植物的损害。本发明提供了SEQ ID NO：1-292或302-304中任一项所述的各种靶多核苷酸或其活性变体和片段，其中目标害虫中一个或多个序列的表达降低控制害虫（即具有杀虫活性）。进一步提供的是沉积元件，当被害虫摄入时，降低靶多肽的水平，从而控制害虫。在具体实施方案中，害虫是五角锥科。还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其它宿主细胞。

3. 发明申请

WO2011153418A2 COMPOSITIONS AND METHODS FOR INSECTICIDAL CONTROL OF STINKBUGS 审中-公开
标题翻译： STINKBUGS的杀虫剂控制组合物和方法
公开(公告)号：WO2011153418A2
公开(公告)日：2011-12-08
申请号：PCT/US2011/039042
申请日：2011-06-03
申请人： BROGLIE, Karen, E. , CERF, David, C. , HERRMANN, Rafael , LU, Albert, L. , McGONIGLE, Brian , PRESNAIL, James, K.
发明人： BROGLIE, Karen, E. , CERF, David, C. , HERRMANN, Rafael , LU, Albert, L. , McGONIGLE, Brian , PRESNAIL, James, K.
IPC分类号： A01H5/00 , C12N15/31 , C12N15/82
CPC分类号： C12N15/8286 , A01N57/16 , A01N65/00 , A01N65/44 , C12N15/113 , C12N15/8218 , C12N2310/14 , C12N2310/531 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Pentatomidae plant pest or a N. viridula, Acrosternum hilare, Piezodorus guildini , and/or Halymorpha halys plant pest, decrease the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: 1-292 or 302-304 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Pentatomidae . Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要翻译：提供了使用沉积元件的方法和组合物，当被害虫摄入时，例如五子科植物害虫或N. viridula，Acrosternum hilare，Piezodorus guildini和/或Halymorpha halys植物害虫，降低靶的表达序列在害虫中。在具体实施方案中，靶序列的表达降低控制有害生物，因此方法和组合物能够限制对植物的损害。本发明提供了SEQ ID NO：1-292或302-304中任一项所述的各种靶多核苷酸或其活性变体和片段，其中目标害虫中一个或多个序列的表达降低控制害虫（即具有杀虫活性）。进一步提供的是沉积元件，当被害虫摄入时，降低靶多肽的水平，从而控制害虫。在具体实施方案中，害虫是五角锥科。还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其它宿主细胞。

4. 发明申请

WO2011059538A1 VIRAL PROMOTER, TRUNCATIONS THEREOF, AND METHODS OF USE 审中-公开
标题翻译：司法促进者，其职责及其使用方法
公开(公告)号：WO2011059538A1
公开(公告)日：2011-05-19
申请号：PCT/US2010/043873
申请日：2010-07-30
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DUPONT DE NEMOURS & COMPANY , DIEHN, Scott , LU, Albert, L. , SIMMONS, Carl, R.
发明人： DIEHN, Scott , LU, Albert, L. , SIMMONS, Carl, R.
IPC分类号： A01H5/00 , C12N15/82 , C12N15/11
CPC分类号： C12N15/8222
摘要： The present disclosure provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
摘要翻译：本公开提供了用于调节植物中异源核苷酸序列表达的组合物和方法。组合物包括用于启动子的新型核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括将可操作地连接到本发明的启动子的核苷酸序列稳定地并入植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。

5. 发明申请

WO2006083513A3 AN INDUCIBLE DEOXYHYPUSINE SYNTHASE PROMOTER FROM MAIZE 审中-公开
标题翻译：一种可诱导的脱氧吉他合成酶促进剂
公开(公告)号：WO2006083513A3
公开(公告)日：2007-03-01
申请号：PCT/US2006001001
申请日：2006-01-11
申请人： PIONEER HI BRED INT , DU PONT , DIEHN SCOTT , LU ALBERT L , MCCUTCHEN BILLY F , SIMS LYNNE E , WARD KIM R
发明人： DIEHN SCOTT , LU ALBERT L , MCCUTCHEN BILLY F , SIMS LYNNE E , WARD KIM R
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12N9/0004 , C12N15/8227 , C12N15/8237 , C12N15/8239
摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for an inducible promoter for the gene encoding a maize deoxyhypusine synthase. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
摘要翻译：本发明提供调节植物中异源核苷酸序列表达的组合物和方法。组合物包括用于编码玉米脱氧酪氨酸合成酶的基因的诱导型启动子的新型核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括将可操作地连接到本发明的根优选启动子的核苷酸序列稳定地并入植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。

6. 发明申请

WO2006076189A3 MAIZE CYCLO1 GENE AND PROMOTER 审中-公开
标题翻译： MAIZE CYCLO1基因和促进剂
公开(公告)号：WO2006076189A3
公开(公告)日：2007-02-22
申请号：PCT/US2006000153
申请日：2006-01-06
申请人： PIONEER HI BRED INT , DU PONT , DIEHN SCOTT , LU ALBERT L , SIMS LYNNE E , WARD KIM R
发明人： DIEHN SCOTT , LU ALBERT L , SIMS LYNNE E , WARD KIM R
IPC分类号： C07K14/415 , A01H5/00 , C12N15/82
CPC分类号： C12N15/8227 , C07K14/415 , C12N15/8279
摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a root-preferred promoter for the gene encoding Cyclo1. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stabling incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence. The present invention also relates to isolated nucleic acids encoding plant cyclotides. The invention relates to the construction of a chimeric gene encoding all or a portion of the plant cyclotides, in sense or antisense orientation, wherein expression of the chimeric gene results in the production of altered levels of plant cyclotides in a transformed host cell.
摘要翻译：本发明提供调节植物中异源核苷酸序列表达的组合物和方法。组合物包括用于编码Cyclo1的基因的根优选启动子的新型核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括将与本发明的根优选启动子可操作地连接的核苷酸序列掺入到植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。本发明还涉及编码植物环肽的分离的核酸。本发明涉及在正义或反义方向上构建编码全部或部分植物环肽的嵌合基因，其中嵌合基因的表达导致在转化的宿主细胞中产生改变水平的植物环肽。

7. 发明申请

WO2006107954A2 METHODS AND COMPOSITIONS FOR DESIGNING NUCLEIC ACID MOLECULES FOR POLYPEPTIDE EXPRESSION IN PLANTS USING PLANT VIRUS CODON-BIAS 审中-公开
标题翻译：使用植物病毒CODON-BIAS设计植物中多肽表达的核酸分子的方法和组合物
公开(公告)号：WO2006107954A2
公开(公告)日：2006-10-12
申请号：PCT/US2006/012478
申请日：2006-04-04
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DU PONT DE NEMOURS & COMPANY , SIMMONS, Carl R. , ABAD, Andre R. , FLANNAGAN, Ronald D. , HERRMANN, Rafael , LU, Albert L. , MCCUTCHEN, Billy F.
发明人： ABAD, Andre R. , FLANNAGAN, Ronald D. , HERRMANN, Rafael , LU, Albert L. , MCCUTCHEN, Billy F.
CPC分类号： C12N15/8216 , C12N15/8286 , Y02A40/162
摘要： The present invention relates to methods of designing nucleic acid molecules for improved expression of the encoded polypeptides in plants. In such methods, codon usage frequencies are biased towards codon usage frequencies of a plant virus, group of plant viruses, or a subset of nucleic acid molecules therefrom. In preferred embodiments, the encoded polypeptide affects the phenotype of the plant. The invention also pertains to nucleic acid molecules encoding insecticidal polypeptides wherein the nucleic acid molecules have been designed to have plant virus codon-biased. The invention also pertains to transgenic plants and progeny thereof with increased expression of insecticidal polypeptides for improved resistance to insects and other pests that are detrimental to plants of agricultural value.
摘要翻译：本发明涉及设计核酸分子以改进植物中编码多肽表达的方法。在这种方法中，密码子使用频率偏向于植物病毒，植物病毒组或其核酸分子的子集的密码子使用频率。在优选的实施方案中，编码的多肽影响植物的表型。本发明还涉及编码杀虫多肽的核酸分子，其中核酸分子被设计为具有植物病毒密码子偏倚。本发明还涉及具有增加的杀虫多肽表达的转基因植物及其后代，以改善对对农业价值的植物有害的昆虫和其它害虫的抗性。

8. 发明申请

WO2005063998A3 MAIZE METALLOTHIONEIN 2 PROMOTER AND METHODS OF USE 审中-公开
标题翻译： MAIZE METALLOTHIONEIN 2促进剂和使用方法
公开(公告)号：WO2005063998A3
公开(公告)日：2005-12-22
申请号：PCT/US2004043412
申请日：2004-12-22
申请人： PIONEER HI BRED INT , DU PONT , DIEHN SCOTT , LU ALBERT , WARD KIM
发明人： DIEHN SCOTT , LU ALBERT , WARD KIM
IPC分类号： A01H1/00 , A01H5/00 , C07H21/04 , C07K14/825 , C12N15/82
CPC分类号： C12N15/8227 , C12N15/8222
摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a promoter for the gene encoding metallothionein. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises transforming a plant or plant cell with a nucleotide sequence operably linked to one of the promoters of the present invention.
摘要翻译：本发明提供调节植物中异源核苷酸序列表达的组合物和方法。组合物包括用于编码金属硫蛋白的基因的启动子的新型核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括用与本发明的启动子之一可操作地连接的核苷酸序列转化植物或植物细胞。

9. 发明申请

WO2003000863A3 DEFENSIN POLYNUCLEOTIDES AND METHODS OF USE 审中-公开
公开(公告)号：WO2003000863A3
公开(公告)日：2003-01-03
申请号：PCT/US2002/021177
申请日：2002-06-21
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E.I. DU PONT DE NEMOURS AND COMPANY , CAHOON, Rebecca, E. , HERRMANN, Rafael , HARVELL, Leslie, T. , LU, Albert, Laurence , MCCUTCHEN, Billy, Fred , NAVARRO ACEVEDO, Pedro, A. , SIMMONS, Carl, R. , WONG, James, F., H.
发明人： CAHOON, Rebecca, E. , HERRMANN, Rafael , HARVELL, Leslie, T. , LU, Albert, Laurence , MCCUTCHEN, Billy, Fred , NAVARRO ACEVEDO, Pedro, A. , SIMMONS, Carl, R. , WONG, James, F., H.
IPC分类号： A01H5/00
摘要： Methods and compositions for mudulating development and defense responses are provided. Nucleotide sequences encoding defensin proteins are provided. The sequences can be used in expression cassettes for mudulating development, developmental pathways, and defense responses. Transformed plants, plant cells, tissues, and seed are also provided.

10. 发明申请

WO2002086072A2 ANIMICROBIAL POLYPEPTIDES AND THEIR USES 审中-公开
标题翻译：动物多肽及其用途
公开(公告)号：WO2002086072A2
公开(公告)日：2002-10-31
申请号：PCT/US2002/012511
申请日：2002-04-19
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E.I. DU PONT DE NEMOURS AND COMPANY , ALTIER, Daniel, J. , HERRMANN, Rafael , LU, Albert, L. , McCUTCHEN, Billy, F. , PRESNAIL, James, K. , WEAVER, Janine, L. , WONG, James, F., H.
发明人： ALTIER, Daniel, J. , HERRMANN, Rafael , LU, Albert, L. , McCUTCHEN, Billy, F. , PRESNAIL, James, K. , WEAVER, Janine, L. , WONG, James, F., H.
IPC分类号： C12N
CPC分类号： C12N15/8282 , C07K14/43563 , Y10S530/858
摘要： The methods and compositions of the present invention find use in impacting microbial pathogens and in enhancing disease resistance to pathogens, particularly by plants. The compositions of the invention include polypeptides that possess antimicrobial properties, particularly fungicidal properties, and the encoding nucleic acid molecules. The polypeptides of the invention are isolated from the hemolymph and fat bodies of insect larvae induced by injection of plant pathogenic fungi. Further provided are plant cells, plants, an seed thereof, transformed with the nucleic acid mole cules of the invention so as to confer disease resistance on the plant.
摘要翻译：本发明的方法和组合物用于影响微生物病原体并增强对病原体的抗病性，特别是植物的抗病性。本发明的组合物包括具有抗微生物特性，特别是杀真菌性质和编码核酸分子的多肽。通过注射植物致病真菌诱导的昆虫幼虫的血淋巴和脂肪体分离本发明的多肽。进一步提供了用本发明的核酸摩尔转化的植物细胞，植物及其种子，从而赋予植物抗病性。

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