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    • 3. 发明申请
    • COMPOSITIONS AND METHODS FOR THE SUPPRESSION OF TARGET POLYNUCLEOTIDES FROM LEPIDOPTERA
    • 用于抑制来自LEPIDOPTERA的目标多核苷酸的组合物和方法
    • WO2009091862A2
    • 2009-07-23
    • PCT/US2009031055
    • 2009-01-15
    • PIONEER HI BRED INTDU PONTHERRMANN RAFAELLASSNER MICHAELLU ALBERT LNELSON MARKPRESNAIL JAMES KRICE JANET A
    • HERRMANN RAFAELLASSNER MICHAELLU ALBERT LNELSON MARKPRESNAIL JAMES KRICE JANET A
    • C12N15/82
    • C12N15/8286A01N65/20A01N65/44C12N15/113C12N2310/11C12N2310/14C12N2310/531C12N2320/32Y02A40/162
    • Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Lepidoptera order, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides target polynucleotides encoding polypeptides from specific protein families and various target polynucleotides set forth in SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Spodoptera frugiperda. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
    • 提供了使用沉积元件的方法和组合物,当沉积元件在被害虫如来自鳞翅目序列的害虫中摄取时,它们能够降低害虫中靶序列的表达。 在具体实施方案中,靶序列的表达降低控制有害生物,因此方法和组合物能够限制对植物的损害。 本发明提供编码来自特定蛋白质家族的多肽的多核苷酸和SEQ ID NO:1,2,3,4,5,6,7,8,9,10,11,12,13,14中所示的各种靶多核苷酸的靶多核苷酸 ,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39 ,40,41,42,43,44,45,46,47,48,49或50个或其活性变体和片段,其中目标害虫中一个或多个序列的表达降低控制害虫(即 ,具有杀虫活性)。 进一步提供的是沉积元件,当被害虫摄入时,降低靶多肽的水平,从而控制害虫。 在具体实施方案中,害虫是草地夜蛾(Spodoptera frugiperda)。 还提供了包含本发明的沉默元件或其活性变体或片段的植物,植物部分,细菌和其它宿主细胞。
    • 6. 发明申请
    • COMPOSITIONS AND METHODS FOR INSECTICIDAL CONTROL OF STINKBUGS
    • STINKBUGS的杀虫剂控制组合物和方法
    • WO2011153418A3
    • 2012-05-31
    • PCT/US2011039042
    • 2011-06-03
    • BROGLIE KAREN ECERF DAVID CHERRMANN RAFAELLU ALBERT LMCGONIGLE BRIANPRESNAIL JAMES K
    • BROGLIE KAREN ECERF DAVID CHERRMANN RAFAELLU ALBERT LMCGONIGLE BRIANPRESNAIL JAMES K
    • A01H5/00C12N15/31C12N15/82
    • C12N15/8286A01N57/16A01N65/00A01N65/44C12N15/113C12N15/8218C12N2310/14C12N2310/531Y02A40/162
    • Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Pentatomidae plant pest or a N. viridula, Acrosternum hilare, Piezodorus guildini, and/or Halymorpha halys plant pest, decrease the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: 1-292 or 302-304 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Pentatomidae. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
    • 提供了使用沉积元件的方法和组合物,当被害虫摄入时,例如五子科植物害虫或N. viridula,Acrosternum hilare,Piezodorus guildini和/或Halymorpha halys植物害虫,降低靶的表达 序列在害虫中。 在具体实施方案中,靶序列的表达降低控制有害生物,因此方法和组合物能够限制对植物的损害。 本发明提供了SEQ ID NO:1-292或302-304中任一项所述的各种靶多核苷酸或其活性变体和片段,其中目标害虫中一个或多个序列的表达降低控制害虫( 即具有杀虫活性)。 进一步提供的是沉积元件,当被害虫摄入时,降低靶多肽的水平,从而控制害虫。 在具体实施方案中,害虫是五角锥科。 还提供了包含本发明的沉默元件或其活性变体或片段的植物,植物部分,细菌和其它宿主细胞。