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1. 发明申请

WO2011075593A1 MAIZE EVENT DP-040416-8 AND METHODS FOR DETECTION THEREOF 审中-公开
标题翻译：主要事项DP-040416-8及其检测方法
公开(公告)号：WO2011075593A1
公开(公告)日：2011-06-23
申请号：PCT/US2010/060841
申请日：2010-12-16
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DUPONT DE NEMOURS & COMPANY , DIEHN, Scott , LU, Albert L. , NOWATZKI, Timothy M. , NUBEL, Douglas Stuart , REGISTER, III, James Calvin , SCELONGE, Christopher Jay , VILLANELO, M. Alejandra Pascual , YOUNG, Gregory James , YOUNG, Joshua K. , ZHONG, Cathy Xiaoyan
发明人： DIEHN, Scott , LU, Albert L. , NOWATZKI, Timothy M. , NUBEL, Douglas Stuart , REGISTER, III, James Calvin , SCELONGE, Christopher Jay , VILLANELO, M. Alejandra Pascual , YOUNG, Gregory James , YOUNG, Joshua K. , ZHONG, Cathy Xiaoyan
IPC分类号： C12N15/82 , A01H1/02 , C07K14/325 , A01H5/00
CPC分类号： C12N15/8286 , A01H1/02 , Y02A40/162 , Y10T436/143333
摘要： The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-040416-8 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.
摘要翻译：本发明提供了涉及转基因抗虫玉米植物的DNA组合物。还提供了基于插入到玉米基因组中的重组构建体的DNA序列和插入位点侧翼的DNA序列来检测玉米DP-040416-8事件的存在的测定法。提供了用于进行测定的试剂盒和条件。

2. 发明申请

WO2011084621A1 MAIZE EVENT DP-004114-3 AND METHODS FOR DETECTION THEREOF 审中-公开
标题翻译：玉米活动DP-004114-3及其检测方法
公开(公告)号：WO2011084621A1
公开(公告)日：2011-07-14
申请号：PCT/US2010/060818
申请日：2010-12-16
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DUPONT DE NEMOURS & COMPANY , DIEHN, Scott , LU, Albert L. , NOWATZKI, Timothy M. , NUBEL, Douglas Stuart , REGISTER, III, James Calvin , SCELONGE, Christopher Jay , VILLANELO, M. Alejandra Pascual , YOUNG, Joshua K. , ZHONG, Cathy Xiaoyan , CROWGEY, Erin
发明人： DIEHN, Scott , LU, Albert L. , NOWATZKI, Timothy M. , NUBEL, Douglas Stuart , REGISTER, III, James Calvin , SCELONGE, Christopher Jay , VILLANELO, M. Alejandra Pascual , YOUNG, Joshua K. , ZHONG, Cathy Xiaoyan , CROWGEY, Erin
IPC分类号： A01H5/10 , C12N15/82 , C07K14/325
CPC分类号： C12Q1/6895 , A01H5/10 , C12N15/8279 , C12Q2600/13
摘要： The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-004114-3 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.
摘要翻译：本发明提供了涉及转基因抗虫玉米植物的DNA组合物。还提供了基于插入到玉米基因组中的重组构建体的DNA序列和插入位点侧翼的DNA序列来检测玉米DP-004114-3事件的存在的测定法。提供了用于进行测定的试剂盒和条件。

3. 发明申请

WO2013019411A1 METHODS AND COMPOSITIONS FOR TARGETED INTEGRATION IN A PLANT 审中-公开
标题翻译：方法和组合在工厂中进行集中整合
公开(公告)号：WO2013019411A1
公开(公告)日：2013-02-07
申请号：PCT/US2012/047202
申请日：2012-07-18
申请人： E. I. DU PONT DE NEMOURS AND COMPANY , PIONEER HI-BRED INTERNATIONAL , CIGAN, Andrew Mark , FALCO, Saverio Carl , GAO, Huirong , LASSNER, Michael W. , LI, Zhongsen , LIU, Zhan-Bin , LYZNIK, Leszek A. , PETERSON, David J. , SCELONGE, Christopher Jay
发明人： CIGAN, Andrew Mark , FALCO, Saverio Carl , GAO, Huirong , LASSNER, Michael W. , LI, Zhongsen , LIU, Zhan-Bin , LYZNIK, Leszek A. , PETERSON, David J. , SCELONGE, Christopher Jay
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12N15/8213
摘要： Compositions and methods are provided for the targeted integration of a polynucleotide sequence of interest into the genome of a plant or plant cell. The methods and compositions employ recognition sites for endonucleases and endonucleases in combination with site-specific recombination sites/recombinases to provide an effective system for establishing target sites within the genome of a plant, plant cell or seed. Once such target sites are established, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest.
摘要翻译：提供组合物和方法用于将目的多核苷酸序列靶向整合到植物或植物细胞的基因组中。方法和组合物使用核酸内切酶和内切核酸酶的识别位点与位点特异性重组位点/重组酶组合，以提供用于在植物，植物细胞或种子的基因组内建立靶位点的有效系统。一旦建立了这样的靶位点，就可以采用多种方法来进一步修饰靶位点，使得它们含有多种感兴趣的多核苷酸。

4. 发明申请

WO0018939A9 METHOD OF PLANT TRANSFORMATION 审中-公开
标题翻译：植物转化方法
公开(公告)号：WO0018939A9
公开(公告)日：2000-09-14
申请号：PCT/US9922379
申请日：1999-09-28
申请人： PIONEER HI BRED INT , BIDNEY DENNIS L , SCELONGE CHRISTOPHER JAY
发明人： BIDNEY DENNIS L , SCELONGE CHRISTOPHER JAY
IPC分类号： C12N15/82 , C12N15/84
CPC分类号： C12N15/8241 , C12N15/8205 , C12N15/8209
摘要： Compositions and methods for the efficient co-transformation of a plant are provided. Novel compositions are Agrobacterium strains that have been engineered to comprise at least two binary vector plasmids in addition to a helper plasmid comprising the vir functions. Each of the binary vectors comprises its own T-DNA borders flanking a heterologous nucleotide sequence of interest. Methods of the invention comprise the use of these novel multiple-binary vector Agrobacterium strains to co-transform a plant. In this manner, heterologous nucleotide sequences of interest residing on different binary vectors can be independently introduced into the plant in a single transformation event and incorporated in the plant's nuclear DNA in an unlinked manner. The invention also provides for regenerated, fertile transgenic plants, transgenic seeds produced therefrom, and T1 and subsequent generations.
摘要翻译：提供了植物有效共同转化的组合物和方法。除了包含vir功能的辅助质粒之外，新型组合物是经过工程改造以包含至少两种二元载体质粒的农杆菌菌株。每个二元载体包含在感兴趣的异源核苷酸序列侧翼的其自身的T-DNA边界。本发明的方法包括使用这些新型多元二元载体农杆菌菌株共同转化植物。以这种方式，驻留在不同二元载体上的感兴趣的异源核苷酸序列可以在单个转化事件中独立地引入植物中，并以非连接的方式并入植物的核DNA中。本发明还提供了再生的，可育的转基因植物，由其产生的转基因种子，以及T1和随后的几代。

5. 发明申请

WO2011084632A1 MAIZE EVENT DP-032316-8 AND METHODS FOR DETECTION THEREOF 审中-公开
标题翻译：主要事项DP-032316-8及其检测方法
公开(公告)号：WO2011084632A1
公开(公告)日：2011-07-14
申请号：PCT/US2010/060832
申请日：2010-12-16
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DUPONT DE NEMOURS & COMPANY , DIEHN, Scott , LU, Albert L. , NOWATZKI, Timothy M. , NUBEL, Douglas Stuart , REGISTER, James Calvin III , SCELONGE, Christopher Jay , VILLANELO, M. Alejandra Pascual , YOUNG, Gregory James , YOUNG, Joshua K. , ZHONG, Cathy Xiaoyan
发明人： DIEHN, Scott , LU, Albert L. , NOWATZKI, Timothy M. , NUBEL, Douglas Stuart , REGISTER, James Calvin III , SCELONGE, Christopher Jay , VILLANELO, M. Alejandra Pascual , YOUNG, Gregory James , YOUNG, Joshua K. , ZHONG, Cathy Xiaoyan
IPC分类号： A01H5/10 , C12N15/82 , C07K14/325
CPC分类号： C12N15/8279 , A01H5/10 , Y10T436/143333
摘要： The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-032316-8 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.
摘要翻译：本发明提供了涉及转基因抗虫玉米植物的DNA组合物。还提供了基于插入到玉米基因组中的重组构建体的DNA序列和插入位点侧翼的DNA序列来检测玉米DP-032316-8事件的存在的测定法。提供了用于进行测定的试剂盒和条件。

6. 发明申请

WO2011075595A1 MAIZE EVENT DP-043A47-3 AND METHODS FOR DETECTION THEREOF 审中-公开
标题翻译：玉米活动DP-043A47-3及其检测方法
公开(公告)号：WO2011075595A1
公开(公告)日：2011-06-23
申请号：PCT/US2010/060846
申请日：2010-12-16
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DUPONT DE NEMOURS & COMPANY , DIEHN, Scott , LU, Albert L. , NOWATZKI, Timothy M. , NUBEL, Douglas Stuart , REGISTER, III, James Calvin , SCELONGE, Christopher Jay , VILLANELO, M. Alejandra Pascual , YOUNG, Gregory James , YOUNG, Joshua K. , ZHONG, Cathy Xiaoyan
发明人： DIEHN, Scott , LU, Albert L. , NOWATZKI, Timothy M. , NUBEL, Douglas Stuart , REGISTER, III, James Calvin , SCELONGE, Christopher Jay , VILLANELO, M. Alejandra Pascual , YOUNG, Gregory James , YOUNG, Joshua K. , ZHONG, Cathy Xiaoyan
IPC分类号： C12N15/82 , C07K14/325 , C12Q1/68 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8286 , C12Q1/6895 , Y02A40/162 , Y10T436/143333
摘要： The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-043A47-3 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.
摘要翻译：本发明提供了涉及转基因抗虫玉米植物的DNA组合物。还提供了基于插入到玉米基因组中的重组构建体的DNA序列和插入位点侧翼的DNA序列来检测玉米DP-043A47-3事件的存在的测定法。提供了用于进行测定的试剂盒和条件。

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