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    • 16. 发明申请
    • AN ENZYME WITH LIPOLYTIC ACTIVITY
    • 具有脂质活性的酶
    • WO1996013580A1
    • 1996-05-09
    • PCT/DK1995000427
    • 1995-10-26
    • NOVO NORDISK A/SSANDAL, ThomasKAUPPINEN, SakariKOFOD, Lene, Venke
    • NOVO NORDISK A/S
    • C12N09/20
    • C12N9/20
    • The invention relates, inter alia, to a DNA construct comprising a DNA sequence which encodes an enzyme exhibiting lipolytic activity and which comprises: a) the DNA sequence shown in SEQ ID No. 1, or b) an analogue of the DNA sequence shown in SEQ ID No. 1 which i) is homologous with the DNA sequence shown in SEQ ID No. 1, and/or ii) hybridizes with the same oligonucleotide probe as the DNA sequence shown in SEQ ID No. 1, and/or iii) encodes a polypeptide which is homologous with the polypeptide encoded by a DNA sequence comprising the DNA sequence shown in SEQ ID No. 1, and/or iv) encodes a polypeptide which is immunologically reactive with an antibody raised against a purified lipolytic enzyme which is encoded by the DNA sequence shown in SEQ ID No. 1 and/or which is derived from Humicola insolens DSM 1800. Also disclosed, inter alia, are: lipolytic enzymes encoded by such DNA constructs; and enzyme preparations, detergent additives and detergent compositions comprising such lipolytic enzymes.
    • 本发明尤其涉及包含编码显示脂肪分解活性的酶的DNA序列的DNA构建体,其包含:a)SEQ ID No.1所示的DNA序列,或b)所示DNA序列的类似物 i)与SEQ ID No.1所示的DNA序列同源的SEQ ID No.1,和/或ii)与与SEQ ID No.1所示的DNA序列相同的寡核苷酸探针杂交,和/或iii) 编码与由包含SEQ ID No.1所示的DNA序列的DNA序列编码的多肽同源的多肽,和/或iv)编码与针对经编码的纯化的脂肪分解酶产生的抗体具有免疫反应性的多肽 通过SEQ ID No.1所示的DNA序列和/或衍生自Humicola insolens DSM 1800的DNA序列。还公开了:由这样的DNA构建体编码的脂肪分解酶; 和酶制剂,洗涤剂添加剂和包含这种脂肪分解酶的洗涤剂组合物。
    • 18. 发明申请
    • AN alpha-GALACTOSIDASE ENZYME
    • 阿尔法糖皮质激素酶
    • WO1994023022A1
    • 1994-10-13
    • PCT/DK1994000138
    • 1994-03-30
    • NOVO NORDISK A/SKNAP, Inge, HelmerHJORT, Carsten, M.HALKIER, TorbenKOFOD, Lene, Venke
    • NOVO NORDISK A/S
    • C12N09/40
    • C12N9/2465
    • A DNA construct comprising a DNA sequence encoding a polypeptide having alpha -galactosidase activity, wherein the DNA sequence a) encodes a polypeptide comprising the amino acid sequence shown in the appended SEQ ID No. 3, or b) is an analogue of the DNA sequence of a), which i) hybridizes with the DNA sequence shown in the appended SEQ ID No. 1 or 2 or an oligonucleotide probe prepared on the basis of said DNA sequence or on the basis of the amino acid sequence shown in SEQ ID No. 3 under the conditions defined herein; ii) encodes a polypeptide reactive with an antibody reacting with at least one epitope of a polypeptide comprising the amino acid sequence shown in the appended SEQ ID No. 3, and/or; iii) encodes a polypeptide being at least 50 % identical with the polypeptide having the amino acid sequence shown in the appended SEQ ID No. 3, as well as an alpha -galactosidase enzyme encoded by the DNA construct.
    • 包含编码具有α-半乳糖苷酶活性的多肽的DNA序列的DNA构建体,其中所述DNA序列a)编码包含所附SEQ ID No.3所示氨基酸序列的多肽,或b)是所述DNA序列的类似物 的a),其i)与所附的SEQ ID No.1或2所示的DNA序列或基于所述DNA序列制备的寡核苷酸探针杂交,或者基于SEQ ID NO:1所示的氨基酸序列 3在本文定义的条件下; ii)编码与与包含所附SEQ ID No.3所示的氨基酸序列的多肽的至少一个表位反应的抗体反应的多肽,和/或; iii)编码与所附的SEQ ID No.3所示的氨基酸序列的多肽具有至少50%同一性的多肽,以及由DNA构建体编码的α-半乳糖苷酶。