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11. 发明申请

WO2003076650A1 METHOD FOR IDENTIFYING ENZYMES WITH ACTIVITY ON INSOLUBLE SUBSTRATES 审中-公开
标题翻译：用于在不溶性底物上鉴定活性的酶的方法
公开(公告)号：WO2003076650A1
公开(公告)日：2003-09-18
申请号：PCT/US2003/007116
申请日：2003-03-05
申请人： GENENCOR INTERNATIONAL, INC. , GEBERT, Mark, S. , KELLIS, James, T, Jr. , SCHELLENBERGER, Volker , YOON, Mee-Young
发明人： GEBERT, Mark, S. , KELLIS, James, T, Jr. , SCHELLENBERGER, Volker , YOON, Mee-Young
IPC分类号： C12Q1/00
CPC分类号： C12Q1/34 , C12Q1/00 , C12Q1/37 , C12Y301/01074 , G01N2333/21 , G01N2500/20
摘要： A high throughput method for screening for enzymes having activity on insoluble substrates includes grinding the substrates to form a powder or particulate substrate which may be filtered to form a uniform sample that may be aliquoted into conventional multiple assay plates or tubes.
摘要翻译：用于筛选在不溶性底物上具有活性的酶的高通量方法包括研磨底物以形成粉末或颗粒基质，其可以被过滤以形成均匀的样品，其可以等分到常规的多个测定板或管中。

12. 发明申请

WO2003076580A2 HIGH THROUGHPUT MUTAGENESIS SCREENING METHOD 审中-公开
标题翻译：高通量MUTAGENESIS筛选方法
公开(公告)号：WO2003076580A2
公开(公告)日：2003-09-18
申请号：PCT/US2003/006908
申请日：2003-03-05
申请人： GENENCOR INTERNATIONAL, INC. , BOTT, Richard, R. , KELLIS, James, T., Jr. , MORRISON, Thomas, B.
发明人： BOTT, Richard, R. , KELLIS, James, T., Jr. , MORRISON, Thomas, B.
IPC分类号： C12N
CPC分类号： C12Y301/01074 , C12N9/18
摘要： The present invention provides mutagenesis methods for protein engineering. In particular, the present inventionprovides mutagenesis methods that include feedback adjustment from systematic result evaluation. More specifically, the invention provides site-saturation mutagenesis methods that screen for variants with one or more desirable protein properties and evaluates screening results to provide feedback for repeat screening and construction of new libraries. The invention further provides cutinase variants that provide improved stability and activity compared with the wild type cutinase.
摘要翻译：本发明提供蛋白质工程的诱变方法。特别地，本发明提供了包括从系统结果评价的反馈调整的诱变方法。更具体地，本发明提供了筛选具有一种或多种所需蛋白质性质的变体的位点饱和诱变方法，并评估筛选结果以提供重复筛选和构建新文库的反馈。本发明还提供了与野生型角质酶相比提供改善的稳定性和活性的角质酶变体。

13. 发明申请

WO2011140316A4 CONSUMER PRODUCTS WITH PROTEASE VARIANTS 审中-公开
标题翻译：消费品与消费者变化
公开(公告)号：WO2011140316A4
公开(公告)日：2012-02-02
申请号：PCT/US2011035319
申请日：2011-05-05
申请人： PROCTER & GAMBLE , DANISCO US INC , SOUTER PHILLIP FRANK , WARD GLENN STEVEN , POULOSE AYROOKARAN JOSEPH , ESTELL DAVID A , KELLIS JAMES T JR , COLLIER KATHERINE D , CASCAO-PEREIRA LUIS GUSTAVO , ALEKSEYEV VIKTOR YURYEVICH , AMIN NEELAM S , YAO JIAN , AUGUSTYN KATHERINE
发明人： SOUTER PHILLIP FRANK , WARD GLENN STEVEN , POULOSE AYROOKARAN JOSEPH , ESTELL DAVID A , KELLIS JAMES T JR , COLLIER KATHERINE D , CASCAO-PEREIRA LUIS GUSTAVO , ALEKSEYEV VIKTOR YURYEVICH , AMIN NEELAM S , YAO JIAN , AUGUSTYN KATHERINE
IPC分类号： C12N9/54 , C11D3/386 , C11D3/39
CPC分类号： C12N9/54 , C11D3/386 , C11D3/38681
摘要： This invention relates to consumer products comprising proteases, particularly cold water proteases and processes for making and using such products. Such consumer products provide improved cleaning, whiteness and/or freshness. Such proteases are derived from a parent enzyme, for example Bacillus lentus, by substitution, insertion and/or deletion of one or more of the parent enzymes' amino acids.
摘要翻译：本发明涉及包含蛋白酶，特别是冷水蛋白酶的消费品以及制造和使用这些产品的方法。这样的消费品提供了改进的清洁，白度和/或新鲜度。这样的蛋白酶通过一种或多种亲本氨基酸的取代，插入和/或缺失而衍生自母体酶，例如迟缓芽孢杆菌。

14. 发明申请

WO2008153925A2 METHODS FOR IMPROVING MULTIPLE PROTEIN PROPERTIES 审中-公开
标题翻译：改善多种蛋白质性质的方法
公开(公告)号：WO2008153925A2
公开(公告)日：2008-12-18
申请号：PCT/US2008/007103
申请日：2008-06-06
申请人： DANISCO US, INC., GENENCOR DIVISION , CASCAO-PEREIRA, Luis, Gustavo , ESTELL, David, A. , KELLIS, James, T., Jr. , POULOSE, Ayrookaran, J.
发明人： CASCAO-PEREIRA, Luis, Gustavo , ESTELL, David, A. , KELLIS, James, T., Jr. , POULOSE, Ayrookaran, J.
IPC分类号： C07K14/435
CPC分类号： C12N9/54 , C11D3/386 , C11D3/38681 , C12N9/2411 , C12N9/2417 , C12N15/1034 , C12Y302/01001 , C12Y304/21062
摘要： The present invention provides efficient methods for obtaining a protein with one or more beneficial attributes in industrial, consumer or pharmaceutical applications. In some preferred embodiments, the present invention provides methods for producing superior enzymes for a given application through screening an abbreviated set of candidate enzymes.
摘要翻译：本发明提供了在工业，消费者或医药应用中获得具有一个或多个有益属性的蛋白质的有效方法。在一些优选的实施方案中，本发明提供了通过筛选候选酶的缩写产生用于给定应用的优良酶的方法。

15. 发明申请

WO9920727A9 CLEANING COMPOSITIONS CONTAINING MULTIPLY-SUBSTITUTED PROTEASE VARIANTS 审中-公开
标题翻译：含有多取代蛋白质变体的清洁组合物
公开(公告)号：WO9920727A9
公开(公告)日：1999-09-02
申请号：PCT/US9822588
申请日：1998-10-23
申请人： PROCTER & GAMBLE , GENENCOR INT , GHOSH CHANCHAL KUMAR , BAECK ANDRE CESAR , OHTANI RYOHEI , BUSCH ALFRED , SHOWELL MICHAEL STANFORD , SCHELLENBERGER VOLKER , KELLIS JAMES T JR , PAECH CHRISTIAN , NADHERNY JOANNE , NAKI DONALD P , COLLIER KATHERINE D , CALDWELL ROBERT M , POULOSE AYROOKARAN J
发明人： GHOSH CHANCHAL KUMAR , BAECK ANDRE CESAR , OHTANI RYOHEI , BUSCH ALFRED , SHOWELL MICHAEL STANFORD , SCHELLENBERGER VOLKER , KELLIS JAMES T JR , PAECH CHRISTIAN , NADHERNY JOANNE , NAKI DONALD P , COLLIER KATHERINE D , CALDWELL ROBERT M , POULOSE AYROOKARAN J
IPC分类号： A23G4/00 , A23G4/12 , A61K8/66 , A61Q11/00 , C11D1/74 , C11D3/37 , C11D3/386 , C12N9/54 , A23G3/30 , A61K7/28 , A61K7/48
CPC分类号： A23G4/123 , A61K8/66 , A61Q11/00 , C11D1/74 , C11D3/3776 , C11D3/386 , C12N9/54 , C12Y304/21062
摘要： The present invention relates to cleaning compositions comprising a protease variant. One cleaning composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at an amino acid residue position corresponding to position 103 of Bacillus amyloliquefaciens subtilisin in combination with a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 1, 3, 4, 8, 9, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 236, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274 and 275 of Bacillus amyloliquefaciens subtilisin; wherein when said protease variant includes a substitution of amino acid residues positions corresponding to positions 103 and 76, there is also a substitution of an amino acid residue at one or more amino acid residue positions other than amino acid residue positions corresponding to positions 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 or 274 of Bacillus amyloliquefaciens subtilisin; and one or more cleaning adjunct materials. Another cleaning composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 62, 212, 230, 232, 252 and 257 of DOLLAR (Bacillus amyloliquefaciens) subtilisin; and one or more cleaning adjunct materials. Methods for using the cleaning compositions are also provided.
摘要翻译：本发明涉及包含蛋白酶变体的清洁组合物。一种清洁组合物包含蛋白酶变体，其包括氨基酸残基与对应于解淀粉芽孢杆菌枯草杆菌蛋白酶的位置103的氨基酸残基处的另一个天然存在的氨基酸残基的取代，以及氨基酸残基与另一天然存在的一个或多个对应于1,2,3,4,8,9,10,12,13,16,17,18,19,20,21,22,24,27,33， 37，38，42，43，48，55，57，58，61，62，68，72，75，76，77，78，79，86，87，89，97，98，99，101，102， 104，106，107，109，111，114，116，117，119，121，123，126，128，130，131，133，134，137，140，141，142，146， 160,166,167,170,173,174,177,181,182,183,184,185,188,192,194,198,203,204,205,206,209,210,211,212,213， 214,215,216,217,218,222,224,227,228,222,224,227,228,230,232,236,237,238,240,242,243,244,245,246,247,248,249,251,252,225,216,217,218,222,224,227,218,222,224,227,218,222,224,227,218,222,224,227,218,222,224,227,218,222,224,227,218,222,224 253,254,25 5,256,257,258,259,260,261,262,263,265,268,269,270,271,272,274和275的解淀粉芽孢杆菌枯草杆菌蛋白酶; 其中当所述蛋白酶变体包括对位置103和76的氨基酸残基置换时，还有一个或多个除对应于位置27,99的氨基酸残基位置之外的氨基酸残基的氨基酸残基的取代，解淀粉芽孢杆菌枯草杆菌蛋白酶的101,104,107,109,123,128,166,204,206,210,216,217,218,222,260,265或274; 和一种或多种清洁辅助材料。另一种清洁组合物包含蛋白酶变体，其包括氨基酸残基与对应于DOLLAR（解淀粉芽孢杆菌）的位置62,212,230,232,252和257的一个或多个氨基酸残基处的另一个天然存在的氨基酸残基取代，枯草杆菌蛋白酶; 以及一种或多种清洁辅助材料。还提供了使用清洁组合物的方法。

16. 发明申请

WO2011072117A1 FABRIC AND HOME CARE PRODUCTS 审中-公开
标题翻译：织物和家庭护理产品
公开(公告)号：WO2011072117A1
公开(公告)日：2011-06-16
申请号：PCT/US2010/059669
申请日：2010-12-09
申请人： THE PROCTER & GAMBLE COMPANY , SOUTER, Philip, Frank , WARD, Glenn, Steven , BASLER, Jashua, Roy , CASCAO-PEREIRA, Luis, Gustavo , ESTELL, David, A. , KELLIS, James, T., Jr. , PISARCHIK, Alexander , POULOUSE, Ayrookaran, J. , TORRES-PAZMINO, Daniel , ALEKSEYEV, Viktor, Yuryevich
发明人： SOUTER, Philip, Frank , WARD, Glenn, Steven , BASLER, Jashua, Roy , CASCAO-PEREIRA, Luis, Gustavo , ESTELL, David, A. , KELLIS, James, T., Jr. , PISARCHIK, Alexander , POULOUSE, Ayrookaran, J. , TORRES-PAZMINO, Daniel , ALEKSEYEV, Viktor, Yuryevich
IPC分类号： C12N9/52 , C11D3/386
CPC分类号： C11D3/38618 , C11D3/38609 , C11D3/38681 , C12N9/50
摘要： This invention relates to fabric and home care products comprising one or more cold water proteases and processes for making and using such products. Such compositions provide improved cleaning and freshness. Such cold water proteases may be derived from parent enzymes, including BPN' subtilisin and subtilisin derived from Bacillus lentus, by substitution, insertion and/or deletion of one or more of the parent enzymes' amino acids.
摘要翻译：本发明涉及包含一种或多种冷水蛋白酶的织物和家庭护理产品以及用于制造和使用这些产品的方法。这些组合物提供改善的清洁和新鲜度。这样的冷水蛋白酶可以通过取代，插入和/或缺失一种或多种亲本氨基酸从母体酶衍生，包括BPN'枯草杆菌蛋白酶和源自迟缓芽孢杆菌的枯草杆菌蛋白酶。

17. 发明申请

WO2011072099A2 COMPOSITIONS AND METHODS COMPRISING PROTEASE VARIANTS 审中-公开
标题翻译：包含蛋白酶变体的组合物和方法
公开(公告)号：WO2011072099A2
公开(公告)日：2011-06-16
申请号：PCT/US2010/059628
申请日：2010-12-09
申请人： DANISCO US INC. , BASLER, Joshua Roy , CASCAO-PEREIRA, Luis Gustavo , ESTELL, David A. , KELLIS, James T. , PISARCHIK, Alexander , POULOSE, Ayrookaran J. , TORRES-PAZMINO, Daniel
发明人： BASLER, Joshua Roy , CASCAO-PEREIRA, Luis Gustavo , ESTELL, David A. , KELLIS, James T. , PISARCHIK, Alexander , POULOSE, Ayrookaran J. , TORRES-PAZMINO, Daniel
IPC分类号： C12N9/64
CPC分类号： C12N9/54 , C11D3/386 , C12N9/52 , C12N9/64 , C12Y304/21062
摘要： The present invention provides protease variants, compositions comprising protease variants, and methods of using such protease variants and compositions.
摘要翻译：本发明提供了蛋白酶变体，包含蛋白酶变体的组合物，以及使用这些蛋白酶变体和组合物的方法。

18. 发明申请

WO2009149144A2 COMPOSITIONS AND METHODS COMPRISING VARIANT MICROBIAL PROTEASES 审中-公开
标题翻译：包含变异微生物蛋白质的组合物和方法
公开(公告)号：WO2009149144A2
公开(公告)日：2009-12-10
申请号：PCT/US2009046066
申请日：2009-06-03
申请人： DANISCO US INC , BASLER JOSHUA R , CASCAO-PEREIRA LUIS G , ESTELL DAVID A , KELLIS JAMES T
发明人： BASLER JOSHUA R , CASCAO-PEREIRA LUIS G , ESTELL DAVID A , KELLIS JAMES T
IPC分类号： C12N9/54 , A23K1/165
CPC分类号： C12N9/54 , A23K20/189 , C11D3/386 , C11D3/38681 , C11D11/0017 , C12N9/52 , C12Y304/21062
摘要： The present invention provides variant subtilisins and compositions comprising at least one variant subtilisin set forth herein, as well as methods for using these variants and compositions. In some embodiments, the present invention provides variant subtilisins suitable for laundry cleaning applications.
摘要翻译：本发明提供了变体枯草杆菌蛋白酶和包含本文所述的至少一种变体枯草杆菌蛋白酶的组合物，以及使用这些变体和组合物的方法。在一些实施方案中，本发明提供适用于洗衣清洁应用的变体枯草杆菌蛋白酶。

19. 发明申请

WO2009061381A3 ALPHA-AMYLASE VARIANTS WITH ALTERED PROPERTIES 审中-公开
标题翻译：具有改性性质的ALPHA-AMYLASE变体
公开(公告)号：WO2009061381A3
公开(公告)日：2009-11-19
申请号：PCT/US2008012413
申请日：2008-11-03
申请人： DANISCO US INC GENENCOR DIV , SHAW ANDREW , RAMER SANDRA , POWER SCOTT D , SHETTY JAYARAMA K , PAULSON BRADLEY , SHARMA VIVEK , WARD DONALD , CASCAO-PEREIRA LUIS G , KELLIS JAMES T
发明人： SHAW ANDREW , RAMER SANDRA , POWER SCOTT D , SHETTY JAYARAMA K , PAULSON BRADLEY , SHARMA VIVEK , WARD DONALD , CASCAO-PEREIRA LUIS G , KELLIS JAMES T
IPC分类号： C12N9/28 , C12N15/75
CPC分类号： C11D3/38681 , C11D3/386 , C12N9/2417 , D06M16/003
摘要： Disclosed are compositions comprising variants of alpha-amylase that have alpha-amylase activity and which exhibit altered properties relative to a parent AmyS-like alpha-amylase from which they are derived. The compositions comprise an additional enzyme such as a phytase. Also disclosed are methods of using the compositions, and kits related thereto.
摘要翻译：公开了包含α-淀粉酶变体的组合物，其具有α-淀粉酶活性，并且相对于其来源的亲本AmyS样α-淀粉酶表现出改变的性质。组合物包含另外的酶如植酸酶。还公开了使用组合物的方法和与其相关的试剂盒。

20. 发明申请

WO2009100102A2 TS23 ALPHA-AMYLASE VARIANTS WITH ALTERED PROPERTIES 审中-公开
标题翻译：具有改性特性的TS23 ALPHA-AMYLASE变体
公开(公告)号：WO2009100102A2
公开(公告)日：2009-08-13
申请号：PCT/US2009/033027
申请日：2009-02-04
申请人： DANISCO US INC., GENENCOR DIVISION , CASCAO-PEREIRA, Luis , CHANG, Claudine , CHOY, Clement , ESTABROOK, Melodie , JONES, Brian, E. , KELLIS, James, T., Jr. , KOLKMAN, Marc , LEEFLANG, Chris , VROEMEN, Casper , WEYLER, Walter
发明人： CASCAO-PEREIRA, Luis , CHANG, Claudine , CHOY, Clement , ESTABROOK, Melodie , JONES, Brian, E. , KELLIS, James, T., Jr. , KOLKMAN, Marc , LEEFLANG, Chris , VROEMEN, Casper , WEYLER, Walter
IPC分类号： C07K14/435
CPC分类号： C12N9/2417 , A21D8/042 , A23K20/189 , A23K50/75 , A23L29/06
摘要： Described are variants (mutants) of a parent alpha-amylase having alpha-amylase activity and exhibiting altered properties relative to the parent alpha-amylase, and methods of use, thereof.
摘要翻译：描述了具有α-淀粉酶活性并且相对于母体α-淀粉酶具有改变的性质的亲本α-淀粉酶的变体（突变体）及其使用方法。

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