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1. 发明申请

WO9920727A9 CLEANING COMPOSITIONS CONTAINING MULTIPLY-SUBSTITUTED PROTEASE VARIANTS 审中-公开
标题翻译：含有多取代蛋白质变体的清洁组合物
公开(公告)号：WO9920727A9
公开(公告)日：1999-09-02
申请号：PCT/US9822588
申请日：1998-10-23
申请人： PROCTER & GAMBLE , GENENCOR INT , GHOSH CHANCHAL KUMAR , BAECK ANDRE CESAR , OHTANI RYOHEI , BUSCH ALFRED , SHOWELL MICHAEL STANFORD , SCHELLENBERGER VOLKER , KELLIS JAMES T JR , PAECH CHRISTIAN , NADHERNY JOANNE , NAKI DONALD P , COLLIER KATHERINE D , CALDWELL ROBERT M , POULOSE AYROOKARAN J
发明人： GHOSH CHANCHAL KUMAR , BAECK ANDRE CESAR , OHTANI RYOHEI , BUSCH ALFRED , SHOWELL MICHAEL STANFORD , SCHELLENBERGER VOLKER , KELLIS JAMES T JR , PAECH CHRISTIAN , NADHERNY JOANNE , NAKI DONALD P , COLLIER KATHERINE D , CALDWELL ROBERT M , POULOSE AYROOKARAN J
IPC分类号： A23G4/00 , A23G4/12 , A61K8/66 , A61Q11/00 , C11D1/74 , C11D3/37 , C11D3/386 , C12N9/54 , A23G3/30 , A61K7/28 , A61K7/48
CPC分类号： A23G4/123 , A61K8/66 , A61Q11/00 , C11D1/74 , C11D3/3776 , C11D3/386 , C12N9/54 , C12Y304/21062
摘要： The present invention relates to cleaning compositions comprising a protease variant. One cleaning composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at an amino acid residue position corresponding to position 103 of Bacillus amyloliquefaciens subtilisin in combination with a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 1, 3, 4, 8, 9, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 236, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274 and 275 of Bacillus amyloliquefaciens subtilisin; wherein when said protease variant includes a substitution of amino acid residues positions corresponding to positions 103 and 76, there is also a substitution of an amino acid residue at one or more amino acid residue positions other than amino acid residue positions corresponding to positions 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 or 274 of Bacillus amyloliquefaciens subtilisin; and one or more cleaning adjunct materials. Another cleaning composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 62, 212, 230, 232, 252 and 257 of DOLLAR (Bacillus amyloliquefaciens) subtilisin; and one or more cleaning adjunct materials. Methods for using the cleaning compositions are also provided.
摘要翻译：本发明涉及包含蛋白酶变体的清洁组合物。一种清洁组合物包含蛋白酶变体，其包括氨基酸残基与对应于解淀粉芽孢杆菌枯草杆菌蛋白酶的位置103的氨基酸残基处的另一个天然存在的氨基酸残基的取代，以及氨基酸残基与另一天然存在的一个或多个对应于1,2,3,4,8,9,10,12,13,16,17,18,19,20,21,22,24,27,33， 37，38，42，43，48，55，57，58，61，62，68，72，75，76，77，78，79，86，87，89，97，98，99，101，102， 104，106，107，109，111，114，116，117，119，121，123，126，128，130，131，133，134，137，140，141，142，146， 160,166,167,170,173,174,177,181,182,183,184,185,188,192,194,198,203,204,205,206,209,210,211,212,213， 214,215,216,217,218,222,224,227,228,222,224,227,228,230,232,236,237,238,240,242,243,244,245,246,247,248,249,251,252,225,216,217,218,222,224,227,218,222,224,227,218,222,224,227,218,222,224,227,218,222,224,227,218,222,224,227,218,222,224 253,254,25 5,256,257,258,259,260,261,262,263,265,268,269,270,271,272,274和275的解淀粉芽孢杆菌枯草杆菌蛋白酶; 其中当所述蛋白酶变体包括对位置103和76的氨基酸残基置换时，还有一个或多个除对应于位置27,99的氨基酸残基位置之外的氨基酸残基的氨基酸残基的取代，解淀粉芽孢杆菌枯草杆菌蛋白酶的101,104,107,109,123,128,166,204,206,210,216,217,218,222,260,265或274; 和一种或多种清洁辅助材料。另一种清洁组合物包含蛋白酶变体，其包括氨基酸残基与对应于DOLLAR（解淀粉芽孢杆菌）的位置62,212,230,232,252和257的一个或多个氨基酸残基处的另一个天然存在的氨基酸残基取代，枯草杆菌蛋白酶; 以及一种或多种清洁辅助材料。还提供了使用清洁组合物的方法。

2. 发明申请

WO0234762A3 METHOD FOR GENERATING A LIBRARY OF OLIGONUCLEOTIDES COMPRISING A CONTROLLED DISTRIBUTION OF MUTATIONS 审中-公开
标题翻译：用于产生包含可控制分布的寡核苷酸的图书馆的方法
公开(公告)号：WO0234762A3
公开(公告)日：2003-03-13
申请号：PCT/US0132046
申请日：2001-10-12
申请人： GENENCOR INT , CALDWELL ROBERT M , SCHELLENBERGER VOLKER
发明人： CALDWELL ROBERT M , SCHELLENBERGER VOLKER
IPC分类号： C07B61/00 , C12N15/10 , C12Q1/68
CPC分类号： C12N15/1031 , C12N15/102 , C12N15/1027 , C40B40/00
摘要： Methods are disclosed for producing libraries of nucleic acid molecules which libraries are derived from a nucleic acid template. The libraries comprise variant nucleic acids which are produced from a mutagenesis strategy using, e.g., a plurality of defined mutagenic and/or non-mutagenic primers and specific reaction conditions which favor the production of varied combinatorial mutants.
摘要翻译：公开了用于产生文库来自核酸模板的核酸分子文库的方法。文库包含使用例如多个确定的诱变和/或非诱变引物和有利于产生不同组合突变体的特异性反应条件的诱变策略产生的变体核酸。

3. 发明申请

WO2005012547A2 MULTIMERIC OXIDOREDUCTASES 审中-公开
标题翻译：多种氧化剂
公开(公告)号：WO2005012547A2
公开(公告)日：2005-02-10
申请号：PCT/US2004024204
申请日：2004-07-27
申请人： GENENCOR INT , CALDWELL ROBERT M , RASHID M HARUNUR , VALLE FERNANDO
发明人： CALDWELL ROBERT M , RASHID M HARUNUR , VALLE FERNANDO
IPC分类号： C07H21/04 , C12N9/02 , C12N9/06 , C12Q
CPC分类号： C12P7/58 , C12N9/0004 , C12N9/002 , C12N15/74 , C12N15/78 , C12P7/60
摘要： The present invention concerns multimeric oxidoreductase complexes which function in the enzymatic conversion of a carbon substrate, said complexes having a dehydrogenase subunit and a cytochrome C subunit. The invention further relates to polynucleotides coding for the multimeric complexes and methods of use thereof.
摘要翻译：本发明涉及在碳底物的酶促转化中起作用的多聚体氧化还原酶复合物，所述复合物具有脱氢酶亚基和细胞色素C亚基。本发明还涉及编码多聚体复合物的多核苷酸及其使用方法。

4. 发明申请

WO2006074005A3 VARIANT HYPOCREA JECORINA CBH2 CELLULASES 审中-公开
标题翻译：变种高锰酸钾CBH2细胞
公开(公告)号：WO2006074005A3
公开(公告)日：2006-11-16
申请号：PCT/US2005047266
申请日：2005-12-22
申请人： GENENCOR INT , AEHLE WOLFGANG , CALDWELL ROBERT M , DANKMEYER LYDIA , GOEDEGEBUUR FRITS , KELEMEN BRADLEY R , MITCHINSON COLIN , NEEFE PAULIEN , TEUNISSEN PAULINE
发明人： AEHLE WOLFGANG , CALDWELL ROBERT M , DANKMEYER LYDIA , GOEDEGEBUUR FRITS , KELEMEN BRADLEY R , MITCHINSON COLIN , NEEFE PAULIEN , TEUNISSEN PAULINE
IPC分类号： C12N9/42 , A23K1/165 , C11D3/386 , C12P7/10 , D06L4/40
CPC分类号： C11D3/38645 , C12N9/2437 , C12P19/14 , C12Y302/01091 , Y02E50/16
摘要： Described herein are variants of H. jecorina CBH2, a Cel6A enzyme. The present invention provides novel cellobiohydrolases that have altered thermostability.
摘要翻译：本文描述了H.cecorina CBH2，Cel6A酶的变体。本发明提供了具有改变的热稳定性的新型纤维二糖水解酶。

5. 发明申请

WO2004060909A3 INCREASING PRODUCTION OF PROTEINS IN GRAM-POSITIVE MICROORGANISMS 审中-公开
标题翻译：增加蛋白质在生物微生物中的生产
公开(公告)号：WO2004060909A3
公开(公告)日：2005-11-03
申请号：PCT/US0337277
申请日：2003-11-17
申请人： GENENCOR INT , CALDWELL ROBERT M , QUAX WILHELMUS J
发明人： CALDWELL ROBERT M , QUAX WILHELMUS J
IPC分类号： C07K14/32 , C12N1/21 , C12N15/31 , C12N15/75 , C12P21/02 , C12P21/06
CPC分类号： C07K14/32 , C07K2319/00 , C12N15/75 , C12P21/02
摘要： The present invention relates to secretion in Gram-positive microorganisms. The present invention provides the nuclei acid and amino acid sequences for the Bacillus subtilis secretion factor SecG. The present invention also provides means for increasing the secretion of heterologous or homologous proteins in Gram-positive microorganisms.
摘要翻译：本发明涉及革兰氏阳性微生物中的分泌。本发明提供枯草芽孢杆菌分泌因子SecG的核酸和氨基酸序列。本发明还提供增加革兰氏阳性微生物中异源或同源蛋白质分泌的方法。

6. 发明申请

WO9920769A3 MULTIPLY-SUBSTITUTED PROTEASE VARIANTS 审中-公开
标题翻译：多选取代蛋白质变体
公开(公告)号：WO9920769A3
公开(公告)日：1999-08-26
申请号：PCT/US9822500
申请日：1998-10-23
申请人： GENENCOR INT , PROCTER & GAMBLE
发明人： SCHELLENBERGER VOLKER , KELLIS JAMES T JR , PAECH CHRISTIAN , NADHERNY JOANNE , NAKI DONALD P , POULOSE AYROOKARAN J , COLLIER KATHERINE D , CALDWELL ROBERT M , BAECK ANDRE C
IPC分类号： A23G4/00 , A23G4/12 , A23K1/165 , A61K8/00 , A61K8/25 , A61K8/36 , A61K8/37 , A61K8/40 , A61K8/41 , A61K8/44 , A61K8/46 , A61K8/60 , A61K8/64 , A61K8/66 , A61Q1/02 , A61Q1/14 , A61Q11/00 , A61Q11/02 , A61Q19/00 , A61Q19/10 , C11D3/386 , C11D3/395 , C11D7/42 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/50 , C12N9/54 , C12N9/56 , C12N9/58 , C12N15/09 , C12N15/57 , C12R1/07
CPC分类号： C12N9/54 , A23G4/123 , A23K20/189 , A61K8/66 , A61K2800/86 , A61Q11/00 , A61Q19/00 , C11D3/386 , C11D3/38609 , C11D3/38618 , C11D3/3907 , C11D3/3945 , C12Y304/00 , C12Y304/21062
摘要： Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue correspond to positions 62, 212, 230, 232, 252 and 257 of Bacillus amyloliquefaciens subtilisin.
摘要翻译：公开了衍生自天然存在或重组非人蛋白酶的DNA序列的新型蛋白酶变体。一般来说，变体蛋白酶通过体外修饰编码天然存在或重组蛋白酶的前体DNA序列获得，以产生前体蛋白酶的氨基酸序列中的多个氨基酸残基的取代。这种变体蛋白酶具有与前体蛋白酶不同的性质，例如改变的洗涤性能。取代的氨基酸残基对应于解淀粉芽孢杆菌枯草杆菌蛋白酶的位置62,212,230,232,252和257。

7. 发明申请

WO9920770A3 MULTIPLY-SUBSTITUTED PROTEASE VARIANTS 审中-公开
标题翻译：多选取代蛋白质变体
公开(公告)号：WO9920770A3
公开(公告)日：1999-09-02
申请号：PCT/US9822572
申请日：1998-10-23
申请人： GENENCOR INT , PROCTER & GAMBLE
发明人： SCHELLENBERGER VOLKER , KELLIS JAMES T JR , PAECH CHRISTIAN , NADHERNY JOANNE , NAKI DONALD P , POULOSE AYROOKARAN J , COLLIER KATHERINE D , CALDWELL ROBERT M , BAECK ANDRE C
IPC分类号： A23G4/00 , A23G4/12 , A23K1/165 , A61K8/00 , A61K8/25 , A61K8/36 , A61K8/37 , A61K8/40 , A61K8/41 , A61K8/44 , A61K8/46 , A61K8/60 , A61K8/64 , A61K8/66 , A61Q1/02 , A61Q1/14 , A61Q11/00 , A61Q11/02 , A61Q19/00 , A61Q19/10 , C11D3/386 , C11D3/395 , C11D7/42 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/50 , C12N9/54 , C12N9/56 , C12N9/58 , C12N15/09 , C12N15/57 , C12R1/07
CPC分类号： C12N9/54 , A23G4/123 , A23K20/189 , A61K8/66 , A61K2800/86 , A61Q11/00 , A61Q19/00 , C11D3/386 , C11D3/38609 , C11D3/38618 , C11D3/3907 , C11D3/3945 , C12Y304/00 , C12Y304/21062
摘要： Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue corresponds to position 103 in combination with one or more of the following substitutions at residue positions corresponding to positions 1, 3, 4, 8, 10, 12, 13, 15, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 236, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274, and 275 of Bacillus amyloliquefaciens subtilisin, wherein when a substitution at a position corresponding to residue position 103 is combined with a substitution at a position corresponding to residue position 76, there is also a substitution at one or more residue positions other than residue positions corresponding to positions 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 or 274 of Bacillus amyloliquefaciens subtilisin.
摘要翻译：公开了衍生自天然存在或重组非人蛋白酶的DNA序列的新型蛋白酶变体。一般来说，变体蛋白酶通过体外修饰编码天然存在或重组蛋白酶的前体DNA序列获得，以产生前体蛋白酶的氨基酸序列中的多个氨基酸残基的取代。这种变体蛋白酶具有与前体蛋白酶不同的性质，例如改变的洗涤性能。取代的氨基酸残基对应于位置103，与对应于位置1,3,4,8,10,12,13,15,16,17,18,19,20的残基位置的一个或多个以下取代组合，21,22,24,27,33,37,38,42,43,48,55,57,58,61,62,68,72,75,76,77,78,79,86,88,98,99 ，97,98,99,101,102,104,106,107,109,111,114,116,117,119,121,123,126,128,130,131,133,134,137,140,141 ，142,146,147,158,159,160,166,167,170,173,174,177,181,182,183,184,185,188,192,194,198,203,204,205,206,206 ，209,210,211,212,213,214,215,216,217,218,222,224,227,228,230,232,236,237,238,240,242,243,244,245,246 ，247,248,249,251,252,253,254,255,256,257,258,259,260,261,262,263,265,268,269,270,271,272,274和275，以及解淀粉芽孢杆菌枯草杆菌蛋白酶，其中当在对应于残基位置103的位置处的取代与对应于t的位置的取代相结合时 o残基位置76，在除位置27,99,101,104,107,109,123,128,166,204,206,210,216,217之外的残基位置之外的一个或多个残基位置也有取代，218,222,260,265或274解淀粉芽孢杆菌枯草杆菌蛋白酶。

8. 发明申请

WO9929876A3 MUTANT BACILLUS LICHENIFORMIS ALPHA-AMYLASE 审中-公开
公开(公告)号：WO9929876A3
公开(公告)日：1999-07-22
申请号：PCT/US9825124
申请日：1998-12-01
申请人： GENENCOR INT
发明人： CALDWELL ROBERT M , MITCHINSON COLIN , ROPP TRACI H
IPC分类号： C12N15/09 , C11D3/386 , C12N1/21 , C12N9/28 , C12N15/00 , C12N15/56 , C12R1/07 , C12R1/10
CPC分类号： C12N9/2417
摘要： Novel alpha -amylase enzymes are disclosed having a substitution equivalent to G475R in Bacillus licheniformis. The disclosed alpha -amylase enzymes show improved specific activity and starch hydrolysis performance.
摘要翻译：公开了新的α-淀粉酶，其在地衣芽孢杆菌中具有等同于G475R的取代。公开的α-淀粉酶显示改善的比活性和淀粉水解性能。

9. 发明申请

WO9424559A3 HOMOGENEOUS IMMUNOASSAYS USING MUTANT GLUCOSE-6-PHOSPHATE DEHYDROGENASES 审中-公开
标题翻译：使用突变葡萄糖-6-磷酸脱氢酶的均质免疫
公开(公告)号：WO9424559A3
公开(公告)日：1995-01-26
申请号：PCT/US9403437
申请日：1994-04-07
申请人： SYNTEX INC , GENENCOR INT
发明人： JAKOBOVITS EDWARD B , SILEN JOY L , LEVY MARK J , GOODMAN THOMAS C , BECKER MARTIN J , ULLMAN EDWIN F , CALDWELL ROBERT M , BOTT RICHARD R , BARNETT CHRISTOPHER CHARLES
IPC分类号： G01N33/542 , C12N9/04 , C12N15/09 , C12Q1/28 , C12Q1/32 , C12R1/19 , G01N33/535 , G01N33/569 , G01N33/58 , G01N33/78 , G01N33/94 , G01N33/53 , G01N33/573
CPC分类号： C12N9/0006 , C12Q1/28 , G01N33/535 , G01N33/56911 , G01N33/581 , G01N33/78 , G01N33/94 , G01N2333/904
摘要： The present invention relates to methods for ummunoassay of analytes employing mutant glucose-6-phosphate dehydrogenase (G6PDH) enzymes as labels. In particular, the invention relates to the use of conjugates of an analyte or analyte analog and a mutant NAD+ dependent G6PDH differing from any precursor G6PDH by the deletion, substitution, or insertion, or any combination thereof of at least one amino acid per subunit. The invention also involves the construction of several mutations in precursor glucose-6-phosphate dehydrogenase (G6PDH) enzymes. Typically, the mutations involve deletion or substitution of one or more lysine residues, or introduction of one or more cysteine residues by insertion of cysteine to precursor G6PDH or substitution of precursor G6PDH amino acids residues with cysteine. The present invention also relates to conjugates of the subject enzymes and specific binding pair members, kits useful in performing the methods of the invention, cell lines producing the subject enzymes, DNA sequences encoding the subject enzymes, and vectors containing DNA encoding the subject enzymes and designed to allow a host cell to produce the subject enzymes.

10. 发明申请

WO9920771A2 MULTIPLY-SUBSTITUTED PROTEASE VARIANTS WITH ALTERED NET CHARGE FOR USE IN DETERGENTS 审中-公开
标题翻译：多用途替代的蛋白质变异体，用于在检测中使用的更换网费
公开(公告)号：WO9920771A2
公开(公告)日：1999-04-29
申请号：PCT/US9822590
申请日：1998-10-23
申请人： GENENCOR INT
发明人： POULOSE AYROOKARAN J , SCHELLENBERGER VOLKER , KELLIS JAMES T JR , PAECH CHRISTIAN , NADHERNY JOANNE , NAKI DONALD P , CALDWELL ROBERT M , COLLIER KATHERINE D
IPC分类号： A23G4/00 , A23G4/12 , A23K1/165 , A61K8/00 , A61K8/25 , A61K8/36 , A61K8/37 , A61K8/40 , A61K8/41 , A61K8/44 , A61K8/46 , A61K8/60 , A61K8/64 , A61K8/66 , A61Q1/02 , A61Q1/14 , A61Q11/00 , A61Q11/02 , A61Q19/00 , A61Q19/10 , C11D3/386 , C11D3/395 , C11D7/42 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/50 , C12N9/54 , C12N9/56 , C12N9/58 , C12N15/09 , C12N15/57 , C12R1/07
CPC分类号： C12N9/54 , A23G4/123 , A23K20/189 , A61K8/66 , A61K2800/86 , A61Q11/00 , A61Q19/00 , C11D3/386 , C11D3/38609 , C11D3/38618 , C11D3/3907 , C11D3/3945 , C12Y304/00 , C12Y304/21062
摘要： Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Protease variants are provided that contain substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more negative or less positive compared to a precursor protease and thus the protease variant is more effective in a low detergent concentration system than a precursor protease. Also provided are protease variants containing substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more positive or less negative compared to a precursor protease and thus the protease variant is more effective in a high detergent concentration system than a precursor protease.
摘要翻译：公开了衍生自天然存在或重组非人蛋白酶的DNA序列的新型蛋白酶变体。一般来说，变体蛋白酶通过体外修饰编码天然存在或重组蛋白酶的前体DNA序列获得，以产生前体蛋白酶的氨基酸序列中的多个氨基酸残基的取代。提供了蛋白酶变体，其含有在一个或多个残基位置处的氨基酸的取代，使得该取代改变该位置的电荷，使得电荷与前体蛋白酶相比更负阴性或更少阳性，因此蛋白酶变体在比前体蛋白酶低的洗涤剂浓度系统。还提供了含有在一个或多个残基位置处的氨基酸取代的蛋白酶变体，使得该取代改变了该位置的电荷，使得电荷比前体蛋白酶更加阳性或更小，因此蛋白酶变体在比前体蛋白酶高的洗涤剂浓度系统。

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