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101. 发明申请

WO2009116566A1 工業的に有用な微生物审中-公开
标题翻译：工业上有用的微生物
公开(公告)号：WO2009116566A1
公开(公告)日：2009-09-24
申请号：PCT/JP2009/055267
申请日：2009-03-18
申请人：協和発酵キリン株式会社 , 桝田貴美枝 , 中村典子 , 溝口寛 , 森英郎
发明人：桝田貴美枝 , 中村典子 , 溝口寛 , 森英郎
IPC分类号： C12N15/09 , C12N1/21 , C12P7/64 , C12P13/04 , C12P19/00 , C12P19/34 , C12P21/02
CPC分类号： C12N1/20 , C07K14/245 , C12N9/0006 , C12N15/70 , C12P7/6463 , C12P13/04 , C12P19/00 , C12P19/34 , C12P21/02
摘要：　本発明の目的は、野生型の大腸菌の染色体ＤＮＡより１０４０kbp以上短い染色体ＤＮＡを有する大腸菌の変異体、および該変異体を用いた有用物質の製造法を提供することができる。
摘要翻译：提供具有比野生型大肠杆菌的染色体DNA短至少1040kbp的染色体DNA的大肠杆菌的突变体，以及使用该变体的有用化合物的制造方法。

102. 发明申请

WO2007087478A8 LINEAR VECTORS, HOST CELLS AND CLONING METHODS 审中-公开
标题翻译：线性矢量，主体细胞和克隆方法
公开(公告)号：WO2007087478A8
公开(公告)日：2009-07-23
申请号：PCT/US2007060500
申请日：2007-01-12
申请人： LUCIGEN CORP , GODISKA RONALD , MEAD DAVID A , RAVIN NIKOLAI V
发明人： GODISKA RONALD , MEAD DAVID A , RAVIN NIKOLAI V
IPC分类号： C12N15/70 , C12N9/90
CPC分类号： C12N15/73 , C12N9/90 , C12N15/70 , C12P19/34
摘要： Linear vectors derived from bacteriophage of E. coli and host cells suitable for cloning are provided. The linear vectors include a left arm comprising a left telomere and a first selectable marker, a right arm comprising a right telomere and a second selectable marker and a cloning region located between the left arm and the right arm. Optional further components of the vector include transcriptional termination sequences, multiple cloning sites and reporter stuffer regions.
摘要翻译：提供了源自大肠杆菌的噬菌体和适合克隆的宿主细胞的线性载体。线性载体包括包含左端粒和第一可选择标记的左臂，包含右端粒和第二可选择标记的右臂和位于左臂和右臂之间的克隆区。载体的任选的其它组分包括转录终止序列，多克隆位点和记录填充区。

103. 发明申请

WO2006107881A3 PRODUCTS AND METHODS FOR REDUCING DYE ARTIFACTS 审中-公开
标题翻译：减少眼睛损伤的产品和方法
公开(公告)号：WO2006107881A3
公开(公告)日：2009-06-04
申请号：PCT/US2006012325
申请日：2006-04-03
申请人： APPLERA CORP , HARROLD MICHAEL , HENNESSY KEVIN , LAU ALDRICH , DESMOND SEAN MATTHEW
发明人： HARROLD MICHAEL , HENNESSY KEVIN , LAU ALDRICH , DESMOND SEAN MATTHEW
IPC分类号： C12P19/34
CPC分类号： C12P19/34 , C12N15/1006 , C12N15/1017 , C12Q1/6848 , C12Q2527/149 , C12Q2523/308 , C12Q2521/101
摘要： The present invention provides products and methods of reducing dye artifacts from chain extension reactions.
摘要翻译：本发明提供了从延伸反应减少染料伪影的产物和方法。

104. 发明申请

WO2006132970A3 METHOD FOR PRODUCING IMPROVED GENE EXPRESSION ANALYSIS AND GENE EXPRESSION ANALYSIS COMPARISON ASSAY RESULTS 审中-公开
标题翻译：用于生产改进的基因表达分析的方法和基因表达分析比较测定结果
公开(公告)号：WO2006132970A3
公开(公告)日：2009-05-07
申请号：PCT/US2006021470
申请日：2006-06-02
申请人： KOHNE DAVID E
发明人： KOHNE DAVID E
IPC分类号： G01N33/48 , C12Q1/00 , G06F19/20
CPC分类号： C12P19/34 , C12Q2600/158 , G06F19/20
摘要： This invention relates to methods and means for producing microarray, non-microarray and clone counting method gene expression and gene expression comparison assay results which are, relative to such prior art produced assay results, known to be significantly improved in normalization and/or assay accuracy and/or biological accuracy, and/or quantitation, and/or interpretability and/or intercomparability, and/or utility. The practice of the invention is necessary to produce microarray, non-microarray, and clone counting method assay measured gene expression and gene expression analysis assay results which can be known to be accurate.
摘要翻译：本发明涉及用于生产微阵列，非微阵列和克隆计数方法基因表达和基因表达比较测定结果的方法和方法，其相对于已知在归一化和/或测定精度中显着提高的现有技术产生的测定结果和/或生物学准确性，和/或定量，和/或可解释性和/或互补性，和/或效用。本发明的实施对于产生微阵列，非微阵列和克隆计数法测定来说是必要的，测定了基因表达和基因表达分析测定结果，其可以被认为是准确的。

105. 发明申请

WO2008078180A3 MATERIALS AND METHODS FOR THE GENERATION OF TRANSCRIPTS COMPRISING MODIFIED NUCLEOTIDES 审中-公开
标题翻译：用于生成包含修饰核苷酸的转录物的材料和方法
公开(公告)号：WO2008078180A3
公开(公告)日：2009-04-23
申请号：PCT/IB2007004146
申请日：2007-12-28
申请人： ARCHEMIX CORP , THOMPSON KRISTIN , DIENER JOHN L , KEEFE ANTHONY DOMINIC
发明人： THOMPSON KRISTIN , DIENER JOHN L , KEEFE ANTHONY DOMINIC
IPC分类号： C12P21/04
CPC分类号： C12N15/115 , C12N9/1247 , C12N15/1048 , C12N2310/16 , C12N2310/321 , C12N2320/51 , C12N2330/30 , C12P19/34 , C12Q1/6865 , C12Q2525/117 , C12Q2521/119 , C12N2310/3521
摘要： Materials and methods are provided for producing aptamer therapeutics having modified nucleotide triphosphates incorporated into their sequence.
摘要翻译：提供材料和方法用于产生具有掺入其序列的修饰的三磷酸核苷酸的适体治疗剂。

106. 发明申请

WO2006107685A3 METHODS, COMPOSITIONS, AND KITS FOR DETECTING NUCLEIC ACIDS IN A SINGLE VESSEL 审中-公开
标题翻译：用于检测单一载体中的核酸的方法，组合物和试剂盒
公开(公告)号：WO2006107685A3
公开(公告)日：2009-04-23
申请号：PCT/US2006011553
申请日：2006-03-30
申请人： EASTMAN CHEM CO
发明人： WISNIEWSKI MICHELE ELIZABETH , BARBOUR WILLIAM MARK , BOYD BRENDAN WILLIAM
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12P19/34
摘要： The invention encompasses processes, compositions, and kits for isolating and detecting nucleic acids from samples using a metal oxide coated onto a vessel. The nucleic acids can be processed and detected within this vessel.
摘要翻译：本发明包括使用涂覆在容器上的金属氧化物从样品中分离和检测核酸的方法，组合物和试剂盒。可以在该容器内处理和检测核酸。

107. 发明申请

WO2009029648A2 METHODS AND COMPOSITIONS FOR PCR 审中-公开
标题翻译：用于PCR的方法和组合物
公开(公告)号：WO2009029648A2
公开(公告)日：2009-03-05
申请号：PCT/US2008/074432
申请日：2008-08-27
申请人： APPLERA CORPORATION , XI, Larry, L. , PROSEN, Dennis, E. , NOVIKOV, Alexander, A.
发明人： XI, Larry, L. , PROSEN, Dennis, E. , NOVIKOV, Alexander, A.
IPC分类号： B32B27/12
CPC分类号： C12N9/1252 , C12N9/88 , C12N9/96 , C12P19/34 , C12Y207/07007 , C12Y402/99
摘要： A modified thermostable Pol B DNA polymerase, produced by a reaction, under essentially aqueous conditions, of a thermostable Pol B DNA polymerase and a modifier reagent of Formula I wherein the reaction results in a thermally reversible inactivation of the thermostable Pol B DNA polymerase activity and the 3'-5' exonuclease activity, which polymerase is suitable for hot-start PCR. Also disclosed are the method for the modification, a polynucleic acid amplification method and PCR reaction mixture and kit comprising the modified thermostable Pol B DNA polymerase.
摘要翻译：通过在基本水溶液条件下通过热稳定的Pol B DNA聚合酶和式I的改性剂通过反应产生的经修饰的热稳定的Pol B DNA聚合酶，其中所述反应导致热可逆的Pol B DNA聚合酶活性的热可逆失活， 3'-5'核酸外切酶活性，该聚合酶适用于热启动PCR。还公开了修饰方法，多核酸扩增方法和PCR反应混合物和试剂盒，其包含修饰的热稳定性Pol B DNA聚合酶。

108. 发明申请

WO2008157688A3 SYNTHESIS AND USE OF ANTI-REVERSE PHOSPHOROTHIOATE ANALOGS OF THE MESSENGER RNA CAP 审中-公开
标题翻译：信使RNA抗体的反向磷酸酯类似物的合成和使用
公开(公告)号：WO2008157688A3
公开(公告)日：2009-02-26
申请号：PCT/US2008067494
申请日：2008-06-19
申请人： UNIV LOUISIANA STATE , JEMIELITY JACEK , GRUDZIEN-NOGALSKA EWA M , KOWALSKA JOANNA , DARZYNKIEWICZ EDWARD , RHOADS ROBERT E
发明人： JEMIELITY JACEK , GRUDZIEN-NOGALSKA EWA M , KOWALSKA JOANNA , DARZYNKIEWICZ EDWARD , RHOADS ROBERT E
IPC分类号： C07H19/207
CPC分类号： C07H19/207 , C12P19/34 , C12P21/02
摘要： New RNA cap analogs are disclosed containing one or more phosphorothioates groups. The analogs also contain modifications at the 2'-O position of 7-methylguanosine that prevent them from being incorporated in the reverse orientation during in vitro synthesis of mRNA and that hence are "anti-reverse cap analogs" (ARCAs). The ARCA modification ensures that the S atom is precisely positioned within the active sites of cap-binding proteins in both the translational and decapping machinery. The new S-ARCA analogs are resistant to in vivo decapping enzymes. Some S-ARCAs have a higher affinity for eIF4E than the corresponding analogs not containing a phosphorothioate group. When mRNAs containing the various S-ARCAs are introduced into cultured cells, some are translated as much as five¬ fold more efficiently than mRNAs synthesized with the conventional analog m7GpppG.
摘要翻译：公开了含有一个或多个硫代磷酸酯基团的新的RNA帽类似物。类似物还含有在7-甲基鸟苷的2'-O位置上的修饰，这些修饰使得它们在体外合成mRNA期间以相反方向并入，因此是“抗反向帽类似物”（ARCAs）。 ARCA修饰确保S原子精确定位于翻译和去盖机器中帽结合蛋白的活性位点。新的S-ARCA类似物对体内脱色酶具有抗性。一些S-ARCAs对eIF4E具有比不含硫代磷酸酯基团的相应类似物更高的亲和力。当含有各种S-ARCAs的mRNA被引入培养的细胞中时，有一些mRNA比用常规的模拟m7GpppG合成的mRNA翻译高5倍。

109. 发明申请

WO2008109176A8 ASSAYS AND OTHER REACTIONS INVOLVING DROPLETS 审中-公开
标题翻译：测定和涉及滴剂的其他反应
公开(公告)号：WO2008109176A8
公开(公告)日：2008-11-13
申请号：PCT/US2008003185
申请日：2008-03-07
申请人： HARVARD COLLEGE , AGRESTI JEREMY , CHU LIANG-YIN , WEITZ DAVID A , KIM JIN-WOONG , ROWAT AMY , SOMMER MORTEN , DANTAS GAUTAM , CHURCH GEORGE
发明人： AGRESTI JEREMY , CHU LIANG-YIN , WEITZ DAVID A , KIM JIN-WOONG , ROWAT AMY , SOMMER MORTEN , DANTAS GAUTAM , CHURCH GEORGE
IPC分类号： C12Q1/68 , B01J13/00
CPC分类号： C12Q1/6848 , B01F3/0807 , B01F3/0811 , B01F13/0062 , B01F13/0071 , B01F2215/0037 , B01J13/0052 , B01J13/0065 , C12P19/34 , C12Q1/6834 , C12Q1/686
摘要： The present invention generally relates to droplets and/or emulsions, such as multiple emulsions. In some cases, the droplets and/or emulsions may be used in assays, and in certain embodiments, the droplet or emulsion may be hardened to form a gel. In some aspects, a heterogeneous assay can be performed using a gel. For example, a droplet may be hardened to form a gel, where the droplet contains a cell, DNA, or other suitable species. The gel may be exposed to a reactant, and the reactant may interact with the gel and/or with the cell, DNA, etc., in some fashion. For example, the reactant may diffuse through the gel, or the hardened particle may liquefy to form a liquid state, allowing the reactant to interact with the cell. As a specific example, DNA contained within a gel particle may be subjected to PCR (polymerase chain reaction) amplification, e.g., by using PCR primers able to bind to the gel as it forms. As the DNA is amplified using PCR, some of the DNA will be bound to the gel via the PCR primer. After the PCR reaction, unbound DNA may be removed from the gel, e.g., via diffusion or washing. Thus, a gel particle having bound DNA may be formed in one embodiment of the invention.
摘要翻译：本发明一般涉及液滴和/或乳液，例如多重乳液。在一些情况下，液滴和/或乳液可以用于测定中，并且在某些实施方案中，液滴或乳液可以被硬化以形成凝胶。在一些方面，可以使用凝胶进行非均相测定。例如，可以使小滴硬化以形成凝胶，其中小滴含有细胞，DNA或其他合适的物质。凝胶可以暴露于反应物，并且反应物可以以某种方式与凝胶和/或与细胞，DNA等相互作用。例如，反应物可以通过凝胶扩散，或者硬化的颗粒可以液化以形成液体状态，从而允许反应物与细胞相互作用。作为具体例子，可以对凝胶颗粒内包含的DNA进行PCR（聚合酶链式反应）扩增，例如通过使用能够在凝胶形成时结合到凝胶上的PCR引物。当使用PCR扩增DNA时，一些DNA将通过PCR引物与凝胶结合。 PCR反应后，未结合的DNA可以从凝胶中去除，例如通过扩散或洗涤。因此，可以在本发明的一个实施方案中形成具有结合DNA的凝胶颗粒。

110. 发明申请

WO2008109176A2 ASSAYS AND OTHER REACTIONS INVOLVING DROPLETS 审中-公开
标题翻译：测量和其他涉及倾销的反应
公开(公告)号：WO2008109176A2
公开(公告)日：2008-09-12
申请号：PCT/US2008003185
申请日：2008-03-07
申请人： HARVARD COLLEGE , AGRESTI JEREMY , CHU LIANG-YIN , WEITZ DAVID A , KIM JIN-WOONG , ROWAT AMY , SOMMER MORTEN , DANTAS GUATAM , CHURCH GEORGE
发明人： AGRESTI JEREMY , CHU LIANG-YIN , WEITZ DAVID A , KIM JIN-WOONG , ROWAT AMY , SOMMER MORTEN , DANTAS GUATAM , CHURCH GEORGE
CPC分类号： C12Q1/6848 , B01F3/0807 , B01F3/0811 , B01F13/0062 , B01F13/0071 , B01F2215/0037 , B01J13/0052 , B01J13/0065 , C12P19/34 , C12Q1/6834 , C12Q1/686
摘要： The present invention generally relates to droplets and/or emulsions, such as multiple emulsions. In some cases, the droplets and/or emulsions may be used in assays, and in certain embodiments, the droplet or emulsion may be hardened to form a gel. In some aspects, a heterogeneous assay can be performed using a gel. For example, a droplet may be hardened to form a gel, where the droplet contains a cell, DNA, or other suitable species. The gel may be exposed to a reactant, and the reactant may interact with the gel and/or with the cell, DNA, etc., in some fashion. For example, the reactant may diffuse through the gel, or the hardened particle may liquefy to form a liquid state, allowing the reactant to interact with the cell. As a specific example, DNA contained within a gel particle may be subjected to PCR (polymerase chain reaction) amplification, e.g., by using PCR primers able to bind to the gel as it forms. As the DNA is amplified using PCR, some of the DNA will be bound to the gel via the PCR primer. After the PCR reaction, unbound DNA may be removed from the gel, e.g., via diffusion or washing. Thus, a gel particle having bound DNA may be formed in one embodiment of the invention.
摘要翻译：本发明通常涉及液滴和/或乳液，例如多重乳液。在一些情况下，液滴和/或乳液可用于测定中，并且在某些实施方案中，液滴或乳液可被硬化以形成凝胶。在一些方面，可以使用凝胶进行异质测定。例如，液滴可以被硬化以形成凝胶，其中液滴包含细胞，DNA或其它合适的物质。凝胶可以暴露于反应物，并且反应物可以以某种方式与凝胶和/或与细胞，DNA等相互作用。例如，反应物可以扩散通过凝胶，或者硬化的颗粒可以液化以形成液体状态，允许反应物与细胞相互作用。作为具体实例，包含在凝胶颗粒内的DNA可以进行PCR（聚合酶链式反应）扩增，例如通过使用能够在形成凝胶时结合凝胶的PCR引物。当使用PCR扩增DNA时，一些DNA将通过PCR引物与凝胶结合。 PCR反应后，未结合的DNA可以从凝胶中去除，例如通过扩散或洗涤。因此，可以在本发明的一个实施方案中形成具有结合DNA的凝胶颗粒。

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