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    • 7. 发明申请
    • DRY AMPLIFICATION REAGENT MIXTURE FOR POLYMERASE CHAIN REACTION AND THE TECHNIQUE OF PCR ANALYSIS
    • 用于聚合酶链反应的干扩散试剂混合物和PCR分析技术
    • WO2005103277A1
    • 2005-11-03
    • PCT/RU2005/000043
    • 2005-02-08
    • OBSCHESTVO S OGRANICHENNOY OTVETSTVENNOSTJU "KOMPANIA "BIOKOM"SCHAIKHAEV, Gadzhiramazan Omarovich
    • SCHAIKHAEV, Gadzhiramazan Omarovich
    • C12P19/34
    • C12Q1/686C12Q2527/149C12Q2527/125
    • To the application for the invention "Dry amplification reagent mixture for polymerase chain reaction and the technique of PCR analysis". The present invention relating to molecular biology and biotechnology refers to dry amplification reagent mixture for polymerase chain reaction (PCR) and the technique of PCR-analysis; it can be used in veterinary, medicine, food industry and other applications relevant to DNA analysis. Dry amplification reagent mixture for PCR contains DNA-polymerase, deoxynucleoside triphosphates, buffer components, electrophoresis water-soluble dye, and stabilizers presented by carbohydrate composition of D-glucose, disaccharide (selected from the group of innulin, sucrose, trehalose maltose), and polysaccharide (selected from the group of D-mannitol, dextrans, phycoll, polyvinyl pyrrolidone). PCR-analysis technique includes dissolving of dry amplification reagent mixture for polymerase chain reaction in buffer solution containing Mg ions with subsequent addition of specific primers and analyzed DNA sample.
    • 对于本发明的应用“用于聚合酶链反应的干扩增试剂混合物和PCR分析技术”。 涉及分子生物学和生物技术的本发明是指用于聚合酶链反应(PCR)的干扩增试剂混合物和PCR分析技术; 它可用于兽医,医药,食品工业等与DNA分析有关的应用。 用于PCR的干扩增试剂混合物包含DNA-聚合酶,脱氧核苷三磷酸,缓冲组分,电泳水溶性染料和由D-葡萄糖,二糖(选自组蛋白,蔗糖,海藻糖麦芽糖)的碳水化合物组成呈现的稳定剂,以及 多糖(选自D-甘露糖醇,葡聚糖,phycoll,聚乙烯吡咯烷酮)。 PCR分析技术包括将含有Mg离子的缓冲溶液中的干扩增试剂混合物溶解于聚合酶链反应中,随后加入特异性引物并分析DNA样品。