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    • 4. 发明申请
    • PROTEASES
    • 蛋白酶
    • WO2004072279A2
    • 2004-08-26
    • PCT/DK2004000091
    • 2004-02-09
    • NOVOZYMES ASPONTOPPIDAN KATRINEWU WENPINGLAN TANGLIU YEMARIA LEONARDO DE
    • PONTOPPIDAN KATRINEWU WENPINGLAN TANGLIU YEDE MARIA LEONARDO
    • C12N9/52C12N9/58C12N15/57A23J3/34C12N9/64
    • C12N9/52A01K2217/05C12N9/58C12N2310/111
    • The invention relates to a sub-class of novel proteases with distinct differences of expected structural relevance to known proteases of Metarhizium and Nocardiopsis. The sub-class encompasses proteases derived from Gliocladium, Periconia, Curvularia, and Brachysporiella. The invention also relates to DNA encoding such proteases, the expression thereof in a host cell, including animal and plant cells, as well as to the use thereof, e.g. in animal feed and in detergents. The invention furthermore relates to the use in animal feed of proteases of at least 55% identity to the protease derived from Brachysporiella. The Metarhizium protease is an The invention relates to a sub-class of novel proteases with distinct differences of expected structural relevance to known proteases of Metarhizium and Nocardiopsis. The sub-class encompasses proteases derived from Gliocladium, Periconia, Curvularia, and Brachysporiella. The invention also relates to DNA encoding such proteases, the expression thereof in a host cell, including animal and plant cells, as well as to the use thereof, e.g. in animal feed and in detergents. The invention furthermore relates to the use in animal feed of proteases of at least 55% identity to the protease derived from Brachysporiella. The Metarhizium protease is an example of such proteases.
    • 本发明涉及具有与已知的绿僵菌和拟诺卡氏菌蛋白酶的预期结构相关性明显不同的新型蛋白酶的亚类。 亚类包括来源于粘帚霉(Gliocladium),Periconia,弯孢属(Curvularia)和短柄螺属(Brachysporiella)的蛋白酶。 本发明还涉及编码这种蛋白酶的DNA,其在包括动物和植物细胞的宿主细胞中的表达,以及其用途,例如, 在动物饲料和洗涤剂中。 本发明还涉及在动物饲料中与来源于短柄螺属的蛋白酶具有至少55%同一性的蛋白酶的用途。 Metarhizium蛋白酶是一种新型蛋白酶的亚类,与已知的绿僵菌和拟诺卡氏菌蛋白酶的预期结构相关性有明显差异。 亚类包括来源于粘帚霉(Gliocladium),Periconia,弯孢属(Curvularia)和短柄螺属(Brachysporiella)的蛋白酶。 本发明还涉及编码这种蛋白酶的DNA,其在包括动物和植物细胞的宿主细胞中的表达,以及其用途,例如, 在动物饲料和洗涤剂中。 本发明还涉及在动物饲料中与来源于短柄螺属的蛋白酶具有至少55%同一性的蛋白酶的用途。 Metarhizium蛋白酶是这类蛋白酶的一个例子。
    • 5. 发明申请
    • THERMOSTABLE ALPHA-AMYLASES
    • 热稳定的α-淀粉酶
    • WO2004055178A1
    • 2004-07-01
    • PCT/DK2003/000882
    • 2003-12-16
    • NOVOZYMES A/STANG, LanWU, WenpingDUAN, JunxinJOHANNESEN, Pia, Francke
    • TANG, LanWU, WenpingDUAN, JunxinJOHANNESEN, Pia, Francke
    • C12N9/30
    • C12N9/242A21D8/042C12C11/003C12N9/2414C12P7/02C12P19/12C12P19/14C12Y302/01001D06M16/003Y02E50/17
    • An isolated polynucleotide comprising an open reading frame encoding a polypeptide having alpha-amylase activity, the polypeptide selected from the group consisting of: a) a polypeptide comprising an amino acid sequencewhich has at least 70% identity with amino acids 22 to 450 of SEQ ID NO:4; b) a polypeptide comprising an amino acid sequence which has at least 70% identity with the polypeptide encoded by the amylase encoding part of the polynucleotide inserted into a plasmid present in the E. coli host deposited under the Budapest Treaty with DSMZ under accession number DSM 15334; c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence which has at least 70% identity with the sequence shown from position 68 to 1417 in SEQ ID NO:3; and d) a fragment of (a), (b) or (c) that has alpha-amylase activity.
    • 包含编码具有α-淀粉酶活性的多肽的开放阅读框的分离的多核苷酸,所述多肽选自:a)包含氨基酸序列的多肽,所述氨基酸序列与以下氨基酸序列具有至少70%的同一性: SEQ ID NO:4的氨基酸22至450; b)包含与由编码多核苷酸的部分的多核苷酸编码的多肽具有至少70%同一性的氨基酸序列的多肽,所述氨基酸序列插入到根据布达佩斯条约以DSMZ保藏的大肠杆菌宿主中存在的质粒中,登录号为DSM 15334; c)由多核苷酸编码的多肽,所述多核苷酸包含与SEQ ID NO:3中第68至1417位所示序列具有至少70%同一性的核苷酸序列; 和d)具有α-淀粉酶活性的(a),(b)或(c)的片段。
    • 9. 发明申请
    • PROTEASES
    • WO2004072279A3
    • 2004-08-26
    • PCT/DK2004/000091
    • 2004-02-09
    • NOVOZYMES A/SPONTOPPIDAN, KatrineWU, WenpingLAN, TangLIU, YeDE MARIA, Leonardo
    • PONTOPPIDAN, KatrineWU, WenpingLAN, TangLIU, YeDE MARIA, Leonardo
    • C12N9/58
    • The invention relates to a sub-class of novel proteases with distinct differences of expected structural relevance to known proteases of Metarhizium and Nocardiopsis. The sub-class encompasses proteases derived from Gliocladium, Periconia, Curvularia, and Brachysporiella. The invention also relates to DNA encoding such proteases, the expression thereof in a host cell, including animal and plant cells, as well as to the use thereof, e.g. in animal feed and in detergents. The invention furthermore relates to the use in animal feed of proteases of at least 55% identity to the protease derived from Brachysporiella. The Metarhizium protease is an The invention relates to a sub-class of novel proteases with distinct differences of expected structural relevance to known proteases of Metarhizium and Nocardiopsis. The sub-class encompasses proteases derived from Gliocladium, Periconia, Curvularia, and Brachysporiella. The invention also relates to DNA encoding such proteases, the expression thereof in a host cell, including animal and plant cells, as well as to the use thereof, e.g. in animal feed and in detergents. The invention furthermore relates to the use in animal feed of proteases of at least 55% identity to the protease derived from Brachysporiella. The Metarhizium protease is an example of such proteases.