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    • 4. 发明申请
    • THERMOSTABLE ALPHA-AMYLASES
    • 热稳定的α-淀粉酶
    • WO2004055178A1
    • 2004-07-01
    • PCT/DK2003/000882
    • 2003-12-16
    • NOVOZYMES A/STANG, LanWU, WenpingDUAN, JunxinJOHANNESEN, Pia, Francke
    • TANG, LanWU, WenpingDUAN, JunxinJOHANNESEN, Pia, Francke
    • C12N9/30
    • C12N9/242A21D8/042C12C11/003C12N9/2414C12P7/02C12P19/12C12P19/14C12Y302/01001D06M16/003Y02E50/17
    • An isolated polynucleotide comprising an open reading frame encoding a polypeptide having alpha-amylase activity, the polypeptide selected from the group consisting of: a) a polypeptide comprising an amino acid sequencewhich has at least 70% identity with amino acids 22 to 450 of SEQ ID NO:4; b) a polypeptide comprising an amino acid sequence which has at least 70% identity with the polypeptide encoded by the amylase encoding part of the polynucleotide inserted into a plasmid present in the E. coli host deposited under the Budapest Treaty with DSMZ under accession number DSM 15334; c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence which has at least 70% identity with the sequence shown from position 68 to 1417 in SEQ ID NO:3; and d) a fragment of (a), (b) or (c) that has alpha-amylase activity.
    • 包含编码具有α-淀粉酶活性的多肽的开放阅读框的分离的多核苷酸,所述多肽选自:a)包含氨基酸序列的多肽,所述氨基酸序列与以下氨基酸序列具有至少70%的同一性: SEQ ID NO:4的氨基酸22至450; b)包含与由编码多核苷酸的部分的多核苷酸编码的多肽具有至少70%同一性的氨基酸序列的多肽,所述氨基酸序列插入到根据布达佩斯条约以DSMZ保藏的大肠杆菌宿主中存在的质粒中,登录号为DSM 15334; c)由多核苷酸编码的多肽,所述多核苷酸包含与SEQ ID NO:3中第68至1417位所示序列具有至少70%同一性的核苷酸序列; 和d)具有α-淀粉酶活性的(a),(b)或(c)的片段。