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1. 发明申请

US20040219622A1 Phosphine-containing formulations for chemiluminescent luciferase assays 审中-公开
标题翻译：用于化学发光荧光素酶测定的含磷配方
公开(公告)号：US20040219622A1
公开(公告)日：2004-11-04
申请号：US10495203
申请日：2004-06-23
发明人： M. Dean Savage
IPC分类号： C12Q001/66 , G01N021/76
CPC分类号： C12Q1/66
摘要： A composition for the chemiluminescent assay of the activity of a luciferase comprising (i) a substrate for the luciferase, which, upon enzymatic reaction with the luciferase in the presence of any required cosubstrate and/or cofactor, yields a detectable chemiluminescent singal, (ii) any cosubstrate and/or cofactor required or beneficial for enzymatic activity of the luciferase, and, as an improvement of the composition, (iii) a water-soluble, organic phosphine-containing compound, which enables the light output from the enzymatic reaction to be modulated. The composition can be used in applications designed to quantitate the presence of the enzyme(s) itself, either in single or dual format, or in applications designed to quantitate the presence of a required cosubstrate, such as ATP.
摘要翻译：用于化学发光测定荧光素酶活性的组合物，其包含（i）用于荧光素酶的底物，其在任何所需的辅助和/或辅因子存在下与萤光素酶酶促反应时产生可检测的化学发光单体（ii））对荧光素酶的酶活性所需或有益的任何辅助和/或辅因子，并且作为组合物的改进，（iii）水溶性有机膦化合物，其使得酶反应的光输出能够被调制。该组合物可用于设计用于以单一或双重格式定量酶本身的存在的应用，或用于定量所需的辅助剂如ATP的存在的应用中。

2. 发明申请

US20040092028A1 Device for enhancing broad band fluorescence with low loss and biological or chemical optic sensor using the same 失效
标题翻译：用于增加具有低损耗的宽带荧光和使用其的生物或化学光学传感器的装置
公开(公告)号：US20040092028A1
公开(公告)日：2004-05-13
申请号：US10450100
申请日：2003-12-29
发明人： Patrick Chaton , Francoise Vinet , Pierre Barritault , Stephane Getin
IPC分类号： G01N021/76
CPC分类号： G01N21/6428 , G01N21/6458 , Y10S435/808
摘要： The invention concerns a device for enhancing fluorescence comprising a support (10) carrying fluorescence enhancement means (11), the fluorescence enhancement means offering a reception surface for chemical or biological elements intended to be read by detection of a fluorescence signal emitted by a fluorophore, associated with the chemical or biological elements, under the effect of an excitation light beam. The fluorescence enhancement means (11) is made up of a thin, transparent, dielectric layer or a stack of thin, transparent, dielectric layers (12 to 16) ensuring a mirror function for the fluorescence signal and excitation light beam, the material of the thin layer or of each thin layer of the stack being chosen from among the following materials: TiO2, Ta2O5, HfO2, ZrO2, MgO, SiO2, Si3N4, MgF2 and YF3. Application to a biological or chemical optic sensor.
摘要翻译：本发明涉及一种用于增强荧光的装置，其包括载有荧光增强装置（11）的载体（10），所述荧光增强装置提供用于通过检测由荧光团发射的荧光信号而被读取的化学或生物元件的接收表面，在激发光束的作用下与化学或生物元素相关联。荧光增强装置（11）由薄的，透明的介电层或薄的，透明的电介质层（12至16）组成，确保了荧光信号和激发光束的反射镜功能，薄层或每层薄层选自以下材料：TiO 2，Ta 2 O 5，HfO 2，ZrO 2，MgO，SiO 2，Si 3 N 4，MgF 2和YF 3。应用于生物或化学光学传感器。

3. 发明申请

US20040053421A1 Chemiluminescent detection of explosives, narcotics, and other chemical substances 有权
标题翻译：爆炸物，麻醉剂和其他化学物质的化学发光检测
公开(公告)号：US20040053421A1
公开(公告)日：2004-03-18
申请号：US10241407
申请日：2002-09-12
发明人： Dao Hinh Nguyen , Stewart Berry , Jan Prot Geblewicz , Guy Couture , Phuong Huynh
IPC分类号： G01N021/76
CPC分类号： G01N21/76 , G01N2001/022 , Y10T436/170769 , Y10T436/173076 , Y10T436/178459
摘要： A compact scanning apparatus has an infrared laser adapted to emit light. The light is delivered as a beam by an optical system to illuminate an interrogation area on the surface of an object being scanned. Such illumination has sufficient intensity and duration to cause selective desorption of molecules of the contraband substance, which are present on the surface, without substantially damaging the surface. A collection system collects at least a portion of the desorbed molecules. At least a portion of the collected molecules is thermally decomposed to form NO2 and transferred to a reaction cell containing an aqueous, alkaline, luminol-containing solution. The NO2 reacts with the luminol to produce light by chemiluminescence. A light detector registers the presence of this light as indicative of the detection of the contraband substance, and activates a signaling device to provide an audible or visible alarm. The apparatus rapidly detects the presence of a wide variety of contraband substances in an accurate, reliable manner. It provides for automated screening, with the result that vagaries of human performance are virtually eliminated. False alarms are reduced and detection efficacy is increased. A traceable residue of the detected contraband is left on the article for use in forensic analysis.
摘要翻译：小型扫描装置具有适于发光的红外激光器。光通过光学系统作为光束传送，以照亮被扫描物体的表面上的询问区域。这种照明具有足够的强度和持续时间，以使得表面上存在的违禁物质的分子的选择性解吸，而不会基本上损害表面。收集系统收集至少一部分解吸的分子。收集的分子的至少一部分被热分解以形成NO 2并转移到含有含水，碱性，含鲁米诺溶液的反应池中。 NO2与鲁米诺反应，通过化学发光产生光。光检测器将该光的存在注册为指示违禁物质的检测，并激活信号装置以提供可听或可见的警报。该装置以准确，可靠的方式快速检测各种违禁物质的存在。它提供了自动化筛选，结果是几乎消除了人类行为的变幻莫测。虚假警报降低，检测效果提高。检测到的违禁品的可追溯残留物留在用于法医分析的物品上。

4. 发明申请

US20040023409A1 Site-specific labelling of proteins using acridone and quinacridone lifetime dyes 审中-公开
标题翻译：使用吖啶酮和喹吖啶酮终身染料的蛋白质的位点特异性标记
公开(公告)号：US20040023409A1
公开(公告)日：2004-02-05
申请号：US10241355
申请日：2002-09-11
发明人： Graham John Cotton
IPC分类号： G01N021/76
CPC分类号： G01N33/582 , C07K7/06 , C09B15/00 , C09B48/00
摘要： The present invention provides reagents and methods that afford direct attachment of a fluorescent acridone or quinacridone dye to either the N-terminus or C-terminus of a synthetic or recombinant peptide or protein, and their derivatives, in a site-specific manner, coupled with purification of the resultant labelled molecule.
摘要翻译：本发明提供试剂和方法，其以荧光吖啶酮或喹吖啶酮染料直接连接到合成或重组肽或蛋白质的N末端或C末端，及其衍生物，以位点特异性方式与纯化得到的标记分子。

5. 发明申请

US20030235924A1 Optoelectronic and microfluidic integration for miniaturized spectroscopic devices 有权
公开(公告)号：US20030235924A1
公开(公告)日：2003-12-25
申请号：US10351294
申请日：2003-01-23
申请人： California Institute of Technology , Office of Technology Transfer
发明人： Mark L. Adams , Stephen R. Quake , Axel Scherer
IPC分类号： G01N021/76
CPC分类号： G01N21/6454 , B01L3/502715 , B01L2300/0654
摘要： Techniques for integrating optoelectronic system and microfluidic system. An apparatus for optical analysis includes a detector system and a microfluidic system on the detector system. The apparatus is free from any lens system between the microfluidic system and the detector system. Methods of making such an apparatus and using such an apparatus are also disclosed.

6. 发明申请

US20030157726A1 Method for improving the measurement accuracy of sensors, especially bio-sensors, which are used to determine the signals of fluorescence radiation 失效
标题翻译：用于提高用于确定荧光辐射信号的传感器，特别是生物传感器的测量精度的方法
公开(公告)号：US20030157726A1
公开(公告)日：2003-08-21
申请号：US10081621
申请日：2002-02-21
发明人： Uwe Blum , Franz Drobner , Peter Boll
IPC分类号： G01N021/76
CPC分类号： G01N21/648 , G01N21/6428 , G01N21/7703 , G01N2021/6439 , G01N2201/127 , G01N2201/12753 , Y10T436/10
摘要： Fluorescing reference material is used for calibration purposes for improving the measurement accuracy of sensors, especially biosensors, which use fluorescence radiation for determination of signals, the sensors each comprising a waveguide having at least one wave propagation surface as assay, a reactant being supported on the reactant comprising a species of molecule and being bound to an analytical material within a test medium, the waveguide transmitting an output emission radiation signal when it receives a radiation access signal.
摘要翻译：荧光参考材料用于校准目的，以提高传感器，特别是生物传感器的测量精度，其使用荧光辐射来确定信号，每个传感器包括具有至少一个波传播表面的波导作为测定，反应物被支撑在反应物，其包含一种分子并且在测试介质内与分析材料结合，该波导在接收到辐射接收信号时传输输出发射辐射信号。

7. 发明申请

US20030113935A1 Analytical device with lightguide illumination of capillary and microgrooves arrays 有权
标题翻译：具有毛细管和微槽阵列的光导照明的分析装置
公开(公告)号：US20030113935A1
公开(公告)日：2003-06-19
申请号：US10028257
申请日：2001-12-19
发明人： Larry J. Carson , Joel R. Dufresne , Patrick R. Fleming , Michael C. Lea , Nicholas A. Lee , John Shigeura
IPC分类号： G01N021/03 , G01N021/76
CPC分类号： G01N21/0303 , G01N21/645 , G01N27/44721 , Y10T436/11
摘要： An analytical cell including a lightguide with a plurality of conduits filled with a migration medium. The medium, the lightguide and a surrounding medium have refractive indices selected such that light entering the lightguide is internally reflected within the lightguide to provide substantially uniform illumination of the conduits.
摘要翻译：一种分析单元，包括具有填充有迁移介质的多个导管的光导。介质，光导和周围介质具有选择的折射率，使得进入光导的光在光导内部被反射以提供导管的基本均匀的照明。

8. 发明申请

US20030092194A1 Luminescence detection workstation 审中-公开
标题翻译：发光检测工作站
公开(公告)号：US20030092194A1
公开(公告)日：2003-05-15
申请号：US10323669
申请日：2002-12-20
发明人： Michael R. Gambini , John C. Voyta , John Atwood , Bruce E. DeSimas II , Edward Lakatos , Jeff Levi , Israel Metal , George Sabak , Yongdong Wang
IPC分类号： G01N021/76
CPC分类号： G01N21/763 , G01N21/15 , G01N21/274 , G01N21/276 , G01N21/31 , G01N21/6452 , G01N21/6456 , G01N21/6486 , G01N21/76 , G01N33/581 , G01N2021/6471 , G01N2333/90241 , Y10T436/115831
摘要： A luminescence detecting apparatus and method for analyzing luminescent samples is disclosed. Luminescent samples are placed in a plurality of sample wells in a tray, and the tray is placed in a visible-light impervious chamber containing a charge coupled device camera. The samples may be injected in the wells, and the samples may be injected with buffers and reagents, by an injector. In the chamber, light from the luminescent samples pass through a collimator, a Fresnel field lens, a filter, and a camera lens, whereupon a focused image is created by the optics on the charge-coupled device (CCD) camera. The use of a Fresnel field lens, in combination with a collimator and filter, reduces crosstalk between samples below the level attainable by the prior art. Preferred embodiments of the luminescence detecting apparatus and method disclosed include central processing control of all operations, multiple wavelength filter wheel, and robot handling of samples and reagents. Preferred embodiments of processing software integrated with the invention include elements for mechanical alignment, outlier shaving, edge detection and masking, manipulation of multiple integration times to expand the dynamic range, crosstalk correction, dark subtraction interpolation and drift correction, multi-component analysis applications specifically tailored for luminescence, and uniformity correction.
摘要翻译：公开了一种用于分析发光样品的发光检测装置和方法。将发光样品放置在托盘中的多个样品孔中，并且将托盘放置在包含电荷耦合器件照相机的可见光不透水室中。样品可以注入孔中，样品可以通过注射器注入缓冲液和试剂。在室中，来自发光样品的光通过准直仪，菲涅尔场透镜，滤光器和照相机镜头，由此电荷耦合器件（CCD）照相机上的光学器件产生聚焦图像。使用菲涅耳场透镜与准直器和滤光器结合使得样品之间的串扰降低到现有技术可获得的水平以下。发光检测装置和方法的优选实施例包括所有操作，多波长滤光轮和样品和试剂的机器人处理的中央处理控制。与本发明集成的处理软件的优选实施例包括用于机械对准，异常刮削，边缘检测和掩蔽的元件，多次积分时间的操纵以扩大动态范围，串扰校正，暗减法内插和漂移校正，特别是多组分分析应用适合发光和均匀性校正。

9. 发明申请

US20020137228A1 Chemiluminescent gas analyzer 失效
标题翻译：化学发光气体分析仪
公开(公告)号：US20020137228A1
公开(公告)日：2002-09-26
申请号：US10102566
申请日：2002-03-20
发明人： Kurt Weckstrom
IPC分类号： G01N021/76
CPC分类号： G01N21/766 , G01N21/76 , Y10T436/177692 , Y10T436/206664 , Y10T436/25875
摘要： This invention relates a chemiluminescent gas analyzer for determining a concentration of a gaseous component (G1) in a sample gas mixture (2). The analyzer comprises a measuring chamber (3), an ozonizer (4) with ozone generating means (44) for producing ozone-containing gas (G2), input conduits (24, 23) for delivering the ozone-containing gas and said gas mixture into the chamber and an outlet (18). A detector (7) receives radiation (E) emitted as a consequence of a reaction between the gaseous component and the ozone-containing gas. Said analyzer further comprises a flow delay device (41) in or upstream the input conduit for said ozone-containing gas and downstream from the ozone generating means (44) of the ozonizer. The flow delay device has a delay volume (VD) causing a predetermined delay time (T) for the flow of the ozone-containing gas from said ozonizer to said measuring chamber (3).
摘要翻译：本发明涉及一种化学发光气体分析仪，用于测定样品气体混合物（2）中气态组分（G1）的浓度。分析仪包括测量室（3），臭氧发生装置（44）的臭氧发生器（4），用于产生含臭氧气体（G2），用于输送含臭氧气体和所述气体混合物的输入管道（24,23）进入腔室和出口（18）。检测器（7）接收由于气体组分和含臭氧气体之间的反应而发射的辐射（E）。所述分析器还包括在用于所述含臭氧的气体的输入管道中或上游的臭氧发生装置（44）的下游的流动延迟装置（41）。所述流动延迟装置具有延迟体积（VD），其导致从所述臭氧发生器向所述测量室（3）流出含臭氧气体的预定延迟时间（T）。

10. 发明申请

US20020132365A1 1,2-Dioxetane derivatives, luminescent reagents, luminescence methods and measuring methods 有权
标题翻译： 1,2-二氧杂环丁烷衍生物，发光试剂，发光方法和测量方法
公开(公告)号：US20020132365A1
公开(公告)日：2002-09-19
申请号：US10091447
申请日：2002-03-07
申请人： TOSOH CORPORATION
发明人： Masakatsu Matsumoto , Nobuko Watanabe , Masashi Yamada
IPC分类号： G01N021/76
CPC分类号： C07F9/6561 , C07F9/65586 , G01N33/582 , Y10T436/142222
摘要： A 1,2-dioxetane derivative of the formula (I): 1 wherein each of R1 and R2 which are independent of each other, is a hydrogen atom, an alkyl group or an aryl group, or R1 and R2 may together form a cyclic or polycyclic organic ring group spiro-bonded to the dioxetane ring, R3 is an alkyl group or an aryl group, or R3 and R1 or R2 may together form a condensed ring containing the dioxetane ring and a hetero atom, and Ar is a group of the formula (A): 2 wherein R4 is a hydroxyl group , an alkoxyl group, an aralkyloxy group, a group of nullOSi(R5R6R7) (wherein each of R5, R6 and R7 which are independent of one another, is an alkyl group alky; group or and aryl group), a phosphate group or a group of nullS(CnullO)R8 (wherein R8 is an alkyl group or an aryl group), each of R9 and R10 which are independent of each other, is a hydrogen atom, an alkyl group, an aryl group or a halogen atom, X is a halogen atom, and V is an oxygen atom or a sulfur atom, or a group of the formula (B): 3 wherein R4 is the same as R in the above formula (A), each of R11 and R12 which are independent of each other, is a hydrogen atom, an alkyl group, an aryl group or a halogen atom, Y is a halogen atom, and W is an oxygen atom or a sulfur atom.
摘要翻译：式（I）的1,2-二氧杂环丁烷衍生物：其中彼此独立的R 1和R 2各自为氢原子，烷基或芳基，或者R 1和R 2可以一起形成环状或与环氧环乙烷环螺环键合的多环有机环基，R3是烷基或芳基，R3和R1或R2可以一起形成含有二氧环乙烷环和杂原子的稠环，Ar是式（A）：其中R 4为羟基，烷氧基，芳烷氧基，-OSi（R 5 R 6 R 7）（其中R 5，R 6和R 7各自独立地为烷基烷基）基团或芳基），磷酸基或-S（C = O）R 8（其中R 8为烷基或芳基）的基团，各自独立地为氢和原子，烷基，芳基或卤素原子，X是卤素原子，V是氧原子或硫原子，或式（B）的基团：其中R4相同作为上述式（A）中的R，R 11和R 12各自独立地为氢原子，烷基，芳基或卤素原子，Y为卤素原子，W为氧原子或硫原子。

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