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1. 发明申请

US20040043439A1 Luciferase detection reagent kits and luciferase detection method using the kit 失效
标题翻译：荧光素酶检测试剂盒和荧光素酶检测方法
公开(公告)号：US20040043439A1
公开(公告)日：2004-03-04
申请号：US10386567
申请日：2003-03-13
申请人： Toyo B-Net Co., Ltd. , TOYO INK MANUFACTURING CO., LTD.
发明人： Masayuki Ryufuku , Hozumi Tanaka , Chie Suzuki
IPC分类号： C12Q001/66
CPC分类号： C12Q1/66
摘要： The object of the present invention is to provide a detection method and a method of manufacturing a detection kit which are characterized by use of an organic sulfur reagent, are effective at low concentration of the reagent, are inexpensive and have reduced unpleasant odor. Disclosed is a reagent kit for detecting a Coleoptera luciferase, comprising an organic sulfur reagent having the atomic sequence of sulfur-carbon-sulfur in its chemical structure, a luciferin, adenosine triphosphate and a magnesium ion. A method for detecting a Coleoptera luciferase, comprising step 1 of mixing an aqueous solution containing an organic sulfur reagent having the atomic sequence of sulfur-carbon-sulfur in its chemical structure, a luciferin, adenosine triphosphate and a magnesium ion with a sample containing a Coleoptera luciferase to give a mixed solution and step 2 of measuring the light emitted in the mixed solution is also disclosed.
摘要翻译：本发明的目的是提供一种检测方法和制造检测试剂盒的方法，其特征在于使用有机硫试剂，在试剂浓度低的情况下是有效的，并且具有降低的令人不快的气味。公开了一种用于检测鞘翅目荧光素酶的试剂盒，其包含其化学结构中具有硫 - 碳 - 硫原子序列的有机硫试剂，荧光素，三磷酸腺苷和镁离子。一种检测鞘翅目荧光素酶的方法，包括步骤1，将含有其化学结构中具有硫 - 碳 - 硫原子序列的有机硫试剂的水溶液，荧光素，三磷酸腺苷和镁离子与含有鞘内荧光素酶得到混合溶液，并且还公开了测量混合溶液中发射的光的步骤2。

2. 发明申请

US20030157519A1 Recombinant renilla reniformis system for bioluminescence resonance energy transfer 审中-公开
标题翻译：用于生物发光共振能量转移的重组肾素肾脏系统
公开(公告)号：US20030157519A1
公开(公告)日：2003-08-21
申请号：US10265031
申请日：2002-10-04
申请人： Stratagene
发明人： Vivan Q. Zhang , Peter E. Vaillancourt
IPC分类号： C12Q001/68 , G01N033/53 , G01N033/537 , G01N033/543 , C12Q001/66 , C12N009/06 , C07H021/04 , C12P021/02 , C12N005/06
CPC分类号： C12N9/0069 , C07K14/43595 , C07K2319/00 , G01N33/542 , G01N2500/02
摘要： The invention relates to compositions comprising a first fusion protein comprising a first polypeptide domain and a R. reniformis luciferase and a second fusion protein comprising a second polypeptide domain and a R. reniformis GFP. The invention also relates to compositions comprising one or more polynucleotides encoding a first fusion protein comprising a first polypeptide domain and a R. reniformis luciferase and a second fusion protein comprising a second polypeptide domain and a R. reniformis GFP. The invention also relates to methods and kits for detecting protein-protein interactions, determining the location of a protein-protein interaction, identifying cells wherein there is a protein-protein interaction of interest, and screening for a candidate modulator that increases or decreases the amount of a protein-protein interaction.
摘要翻译：本发明涉及包含第一融合蛋白质的组合物，所述第一融合蛋白质包含第一多肽结构域和罗氏扁桃体荧光素酶，以及第二融合蛋白，其包含第二多肽结构域和红纹氏杆菌GFP。本发明还涉及包含一种或多种编码包含第一多肽结构域和雷氏镜状荧光素酶的第一融合蛋白的多核苷酸的组合物，以及包含第二多肽结构域和红纹氏杆菌GFP的第二融合蛋白。本发明还涉及用于检测蛋白质 - 蛋白质相互作用的方法和试剂盒，确定蛋白质 - 蛋白质相互作用的位置，鉴定存在感兴趣的蛋白质 - 蛋白质相互作用的细胞，以及筛选增加或减少量的候选调节剂的蛋白质 - 蛋白质相互作用。

3. 发明申请

US20030064394A1 ATP-regeneration reaction system, and method for examining adenine nucleotide, method for detecting RNA and method for amplifying ATP, using the same 审中-公开
标题翻译： ATP再生反应体系及检测腺苷核苷酸的方法，检测RNA的方法及其扩增方法
公开(公告)号：US20030064394A1
公开(公告)日：2003-04-03
申请号：US10188091
申请日：2002-07-03
申请人： SATAKE CORPORATION
发明人： Hisao Ohtake , Akio Kuroda , Shotaro Tanaka
IPC分类号： C12Q001/68 , C12Q001/66 , C12P019/30
CPC分类号： C12Q1/66 , C12P19/32 , C12Q1/485
摘要： The present invention relates to an ATP-regeneration reaction system comprising the steps of acting adenylate kinase on AMP to convert it into ADP and acting a polyphosphoric acid synthetase in the presence of a polyphosphoric acid compound to convert it into ATP and a polyphosphoric acid compound; an ATP-regeneration reaction system comprising the steps of acting a phosphotransferase on AMP in the presence of a polyphosphoric acid compound to convert it into ADP and then acting a polyphosphoric acid synthetase on the resulting ADP to convert it into ATP; and a method for detecting or inspecting adenine nucleotide or RNA and an ATP-amplification method, which make use of the foregoing regeneration system.
摘要翻译：本发明涉及一种ATP再生反应系统，其包括在AMP上作用腺苷酸激酶以将其转化成ADP并在多磷酸化合物存在下作用多磷酸合成酶以将其转化为ATP和多磷酸化合物的步骤; 一种ATP再生反应系统，包括以下步骤：在多磷酸化合物的存在下，在磷酸转移酶上进行磷酸转移酶转化成ADP，然后在所得ADP上作用多磷酸合成酶将其转化成ATP; 以及利用上述再生系统检测或检测腺嘌呤核苷酸或RNA的方法以及ATP扩增方法。

4. 发明申请

US20030008338A1 Assay methods and kits therefor 审中-公开
标题翻译：测定方法和试剂盒
公开(公告)号：US20030008338A1
公开(公告)日：2003-01-09
申请号：US10005189
申请日：2001-12-07
申请人： LumiTech (UK) Limited
发明人： Sharon Patricia Mary Crouch , Kevin John Slater
IPC分类号： C12Q001/66 , C12Q001/48
CPC分类号： C12Q1/66 , C12Q1/485 , G01N33/5014
摘要： The invention relates to methods for detecting the cytotoxic activity of an effector, such as a cytotoxic T lymphocyte, on a non-microbial target cell. Detection of cytotoxic activity is preferably achieved by detecting adenylate kinase release using photometric methods.
摘要翻译：本发明涉及用于检测效应物如细胞毒性T淋巴细胞在非微生物靶细胞上的细胞毒活性的方法。细胞毒活性的检测优选通过使用光度法检测腺苷酸激酶释放来实现。

5. 发明申请

US20030003435A1 Methods for delivering nucleic acid molecules into cells and assessment thereof 失效
公开(公告)号：US20030003435A1
公开(公告)日：2003-01-02
申请号：US09815981
申请日：2001-03-22
申请人： CHROMOS MOLECULAR SYSTEMS, INC.
发明人： Gary DeJong , Sandra Louise Vanderbyl
IPC分类号： C12Q001/00 , C12Q001/68 , G01N033/53 , G01N033/567 , C12Q001/66 , C12N015/87
CPC分类号： C12N15/88 , A61K41/0047 , C12N15/63
摘要： Methods for delivering nucleic acid molecules into cells and methods for measuring nucleic acid delivery into cells and the expression of the nucleic acids are provided. The methods are designed for introduction of large nucleic acid molecules, including artificial chromosomes, into cells, and are practiced in vitro and in vivo.

6. 发明申请

US20020132279A1 Formula to manipulate blood glucose via the calculated ingestion of carbohydrate 审中-公开
标题翻译：通过计算摄入的碳水化合物来调节血糖的配方
公开(公告)号：US20020132279A1
公开(公告)日：2002-09-19
申请号：US09766427
申请日：2001-01-18
发明人： Linda Hockersmith
IPC分类号： C12Q001/66 , C12Q001/54 , G06F019/00
CPC分类号： A61B5/14532
摘要： A formula for calculating the amount of carbohydrate necessary to achieve a target blood glucose excursion in a diabetic test subject is based on a baseline blood glucose level, a target level to be achieved and an index of the subject's sensitivity to carbohydrate. Initially, the index value is an exemplary value based on typical carbohydrate sensitivities displayed by various types of diabetics. However, the index may be individualized to a test subject based on an actual glucose excursion. A method of effecting a shift in blood glucose level in a diabetic subject incorporates the formula presented above. Furthermore, a method for dietary management of a diabetic individual's glycemic profile, wherein an optimal glycemic profile is achieved and maintained, also incorporates the formula.
摘要翻译：用于计算在糖尿病测试对象中实现目标血糖偏移所需的碳水化合物量的公式是基于基线血糖水平，待实现的目标水平和受试者对碳水化合物的敏感性的指标。最初，指数值是基于各种糖尿病患者显示的典型碳水化合物敏感性的示例性值。然而，基于实际的葡萄糖偏移，该指数可以被个体化为测试对象。糖尿病患者血糖水平变化的方法包含上述公式。此外，实现和维持最佳血糖分布的糖尿病个体血糖曲线的饮食管理方法也包含该公式。

7. 发明申请

US20020012956A1 METHOD FOR PRODUCING A STABLE TROPONIN PREPARATION AND THE USE THEREOF AS A CALIBRATOR/CONTROL IN IMMUNOASSAYS 失效
标题翻译：生产稳定的三宝龙制剂的方法及其作为免疫测定中的校准/控制的用途
公开(公告)号：US20020012956A1
公开(公告)日：2002-01-31
申请号：US08950240
申请日：1997-10-14
发明人： MARGIT DOTH , CHRISTOPH PETRY
IPC分类号： C12Q001/66
CPC分类号： G01N33/6887 , G01N2333/4712 , Y10S435/965 , Y10S435/967 , Y10S435/972 , Y10S436/811 , Y10S436/815 , Y10S530/807 , Y10T436/10 , Y10T436/102499 , Y10T436/103332 , Y10T436/104165 , Y10T436/105831 , Y10T436/106664 , Y10T436/107497 , Y10T436/108331
摘要： The present invention relates to methods for the production of a stable troponin preparation and its use as a calibrator and/or control in immunoassays. The formulation is prepared from mammalian, preferably bovine, heart tissue which provides a calibrator/control composition which remains stable over a long period of time.
摘要翻译：本发明涉及生产稳定的肌钙蛋白制剂的方法及其在免疫测定中作为校准物和/或对照物的用途。制剂由哺乳动物，优选牛的心脏组织制备，其提供在长时间内保持稳定的校准物/对照组合物。

8. 发明申请

US20010024790A1 DNA base sequencing system 有权
标题翻译： DNA碱基测序系统
公开(公告)号：US20010024790A1
公开(公告)日：2001-09-27
申请号：US09805240
申请日：2001-03-14
申请人： Hitachi, Ltd.
发明人： Hideki Kambara , Guohua Zhou , Yuji Miyahara
IPC分类号： C12Q001/68 , C12Q001/66
CPC分类号： C12Q1/6869 , C12Q2565/537 , C12Q2565/301
摘要： The present invention provides a DNA base sequencing system having a compact, simple and convenient structure. In one embodiment of the present invention, a reaction chamber module for pyrosequencing in which a multiple number of reaction vessels (reaction chambers) and reagent-introducing narrow tubes 6 are integrated is formed in a device board 5. Reagents held in reagent reservoirs 1, 2, 3 and 4 mounted separately from this reaction chamber module are introduced into the reaction vessels 10 via reagent-introducing narrow tubes (capillaries) 6. The reagent-introducing narrow tubes (capillaries) 6 at the area of 2 cm from the reaction vessels 10 are structured with narrow capillaries having an inner diameter of about 0.1 mm and the conductance of these capillaries for the reagent solution determines the injection speed of the solution. Using the present invention, many kinds of DNAs can be analyzed simultaneously.
摘要翻译：本发明提供了具有紧凑，简单和方便结构的DNA碱基测序系统。在本发明的一个实施方案中，在装置基板5上形成有用于焦磷酸测序的反应室模块，其中多个反应容器（反应室）和试剂导入窄管6一体化。与反应室模块分开安装的2,3和4通过试剂引入窄管（毛细管）6引入反应容器10中。在反应容器2cm处的试剂引入窄管（毛细管）6 10具有内径为约0.1mm的窄毛细管，并且用于试剂溶液的这些毛细管的电导决定了溶液的注射速度。使用本发明，可以同时分析多种DNA。

9. 发明申请

US20040241777A1 Luminescent Reaction Measurement Device 失效
标题翻译：发光反应测定装置
公开(公告)号：US20040241777A1
公开(公告)日：2004-12-02
申请号：US10474716
申请日：2004-07-23
发明人： Yoshihito Suzuki , Akifumi Kamiya
IPC分类号： C12Q001/66 , C12M001/34
CPC分类号： H01J43/20 , G01N21/76
摘要： Light resulting from chemiluminescence inside a reaction chamber 4 is transmitted through a light transmitting window 3, a large part of the light is made incident on a photoelectric surface 71 of a photomultiplier tube 2 to generate photoelectrons, and these photoelectrons are successively multiplied by surfaces 79 at one side of dynodes 78a, 78, and 78b. Meanwhile, a part of the light that is not made incident on photoelectric surface 71 is made incident on and reflected by a surface 77 at the other side of dynode 78a, returned to reaction chamber 4 via light transmitting window 3, reflected further by light reflecting surfaces 40 and 41 inside reaction chamber 4, emitted again from light transmitting window 3, and made incident on photoelectric surface 71 of photomultiplier tube 2 to generate and multiply photoelectrons in the same manner as the above. The amount of light made incident on photoelectric surface 71 is thus increased by the amount corresponding to the reflection at surface 77 at the other side of the dynode, thereby enabling detection of light caused by weak chemiluminescence.
摘要翻译：由反应室4内的化学发光产生的光透过透光窗3，大部分光入射到光电倍增管2的光电面71上，产生光电子，这些光电子依次乘以表面79 在倍增极78a，78和78b的一侧。同时，未入射到光电表面71上的光的一部分入射并在倍增极78a另一侧的表面77反射，并经由透光窗3返回到反应室4，进一步被光反射反应室4内的表面40和41再次从透光窗3发射，并入射到光电倍增管2的光电表面71上，以与上述相同的方式产生和乘以光电子。因此，入射到光电表面71上的光量增加了与倍增极的另一侧的表面77处的反射相对应的量，从而能够检测由弱化学发光引起的光。

10. 发明申请

US20040224377A1 Compositions and methods to quench light from optical reactions 审中-公开
标题翻译：从光学反应中淬灭光的组成和方法
公开(公告)号：US20040224377A1
公开(公告)日：2004-11-11
申请号：US10777461
申请日：2004-02-12
发明人： Erika Hawkins , Braeden Butler , Keith V. Wood
IPC分类号： C12Q001/66
CPC分类号： G01N33/543 , C12Q1/66 , G01N33/542
摘要： The present invention relates to single and dual reporter luminescence assays utilizing reagents to quench an optical, e.g., an enzyme-mediated luminescence, reaction. In one embodiment of the invention, a reagent is added to an assay which selectively quenches a first enzyme-mediated luminescence reaction without affecting a subsequent distinct enzyme-mediated luminescent reaction(s). An assay kit containing one or more selective quench reagents, and compositions comprising the quench reagent(s), are also provided.
摘要翻译：本发明涉及使用试剂淬灭光学例如酶介导的发光反应的单和双重报道发光测定。在本发明的一个实施方案中，将试剂加入到选择性淬灭第一酶介导的发光反应而不影响随后不同的酶介导的发光反应的测定中。还提供了含有一种或多种选择性骤冷试剂和包含骤冷试剂的组合物的测定试剂盒。

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