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1. 发明授权

US08318922B2 Treatment and prevention of hyperproliferative conditions in humans and antisense oligonucleotide inhibition of human replication-initiation proteins 有权
标题翻译：治疗和预防人类过度增殖病症和反义寡核苷酸抑制人类复制起始蛋白质
公开(公告)号：US08318922B2
公开(公告)日：2012-11-27
申请号：US12126862
申请日：2008-05-24
申请人： Chun Liang , Dao-rong Feng , Zhi-ling Yu
发明人： Chun Liang , Dao-rong Feng , Zhi-ling Yu
IPC分类号： C07H21/00 , C07H21/02 , C07H2/04
CPC分类号： C12N15/113 , A61K38/00 , C12N2310/11 , C12N2310/315
摘要： Antisense oligonucleotides that inhibit expression of human replication-initiation protein as well as methods of preventing or treating hyperproliferative conditions using said oligonucleotides are disclosed. One aspect provides an antisense oligonucleotide that inhibits the expression of human replication-initiation protein and has a sequence complementary to at least a portion of a target sequence encoding a human replication-initiation gene. By administering a therapeutically effective amount of an antisense oligonucleotide or by contacting the hyperproliferating cells with an effective amount of one or more antisense oligonucleotides, expression of replication-initiation protein is inhibited. Methods of screening and testing active antisense oligonucleotides for their ability to inhibit gene expression are also disclosed.
摘要翻译：公开了抑制人复制起始蛋白表达的反义寡核苷酸以及使用所述寡核苷酸预防或治疗过度增殖病症的方法。一方面提供了抑制人复制起始蛋白的表达的反义寡核苷酸，并且具有与编码人复制起始基因的靶序列的至少一部分互补的序列。通过施用治疗有效量的反义寡核苷酸或通过使过度增殖细胞与有效量的一种或多种反义寡核苷酸接触，抑制复制起始蛋白的表达。还公开了筛选和测试活性反义寡核苷酸抑制基因表达能力的方法。

2. 发明授权

US07700320B2 Schizochytrium fatty acid synthase (FAS) and products and methods related thereto 失效
标题翻译：裂殖壶菌脂肪酸合成酶（FAS）及其相关的产品和方法
公开(公告)号：US07700320B2
公开(公告)日：2010-04-20
申请号：US11058046
申请日：2005-02-14
申请人： James G. Metz , Craig A. Weaver , Jerry Kuner
发明人： James G. Metz , Craig A. Weaver , Jerry Kuner
IPC分类号： C12Q1/68 , C07H2/04 , C12P7/64 , C12N9/80
CPC分类号： C12N9/1029
摘要： Disclosed are a fatty acid synthase (FAS) from Schizochytrium, biologically active fragments and homologues thereof, a nucleic acid sequence encoding such FAS, fragments and homologues thereof, the gene encoding Schizochytrium FAS, host cells and organisms that recombinantly express the FAS, host cells and organisms in which the expression and/or activity of the endogenous FAS has been attenuated, and various methods for making and using any of these proteins, nucleic acid molecules, genes, host cells or organisms.
摘要翻译：公开了来自裂殖壶菌的脂肪酸合成酶（FAS），其生物活性片段及其同源物，编码这种FAS的核酸序列，其片段和同系物，编码裂殖酵母FAS的基因，重组表达FAS的宿主细胞和生物体宿主细胞和其中内源性FAS的表达和/或活性已被减弱的生物体，以及用于制备和使用这些蛋白质，核酸分子，基因，宿主细胞或生物体中的任何一种的各种方法。

3. 发明授权

US07060876B2 Method for transforming monocotyledons 失效
标题翻译：单子叶植物的转化方法
公开(公告)号：US07060876B2
公开(公告)日：2006-06-13
申请号：US09229324
申请日：1999-01-13
申请人： Yukoh Hiei , Toshihiko Komari
发明人： Yukoh Hiei , Toshihiko Komari
IPC分类号： A01H1/00 , A01H5/00 , C07H2/04 , C12N15/82 , C12N15/84
CPC分类号： C12N15/8205
摘要： The invention relates to a method for transforming a monocotyledonous plant. The time required from transformation to regeneration of a plant is shorter using the inventive method so that the frequency of emergence of mutants is smaller than the conventional methods. The inventive method may be generally applied even to the plants for which a regeneration method from a protoplast to a plant has not been established, and with which the preparation of the material to be subjected to the method is easy. That is, the present invention provides a method for transforming a monocotyledonous plant, comprising contacting a cultured tissue of said monocotyledonous plant during dedifferentiation thereof obtained by culturing an explant on a dedifferentiation-inducing medium for less than 7 days with a bacterium belonging to the genus Agrobacterium containing a super binary vector having the virulence region of a Ti plasmid, left and right border sequences of T-DNA of a Ti plasmid or an Ri plasmid of a bacterium belonging to the genus Agrobacterium, and a desired gene located between said left and right border sequences.
摘要翻译：本发明涉及一种用于转化单子叶植物的方法。使用本发明的方法从植物的转化到再生所需的时间更短，使得突变体的出现频率比常规方法小。本发明的方法通常可以应用于从原生质体到植物的再生方法尚未建立的植物，并且通过该方法对待处理的材料的制备是容易的。也就是说，本发明提供了一种用于转化单子叶植物的方法，包括使所述单子叶植物的去分化过程中的培养的组织与通过在去分化诱导培养基上培养少于7天的外植体与属于该属的细菌接触含有具有Ti质粒的毒力区域的超级二元载体的农杆菌，Ti质粒的T-DNA的左侧和右侧边界序列或属于农杆菌属的细菌的Ri质粒，以及位于所述左侧和右边界序列

4. 发明授权

US07820407B2 Schizochytrium fatty acid synthase (FAS) and products and methods related thereto 失效
公开(公告)号：US07820407B2
公开(公告)日：2010-10-26
申请号：US12434542
申请日：2009-05-01
申请人： James G. Metz , Craig A. Weaver , Jerry Kuner
发明人： James G. Metz , Craig A. Weaver , Jerry Kuner
IPC分类号： C12Q1/68 , C07H2/04 , C12P7/64 , C12N9/80
CPC分类号： C12N9/1029
摘要： Disclosed are a fatty acid synthase (FAS) from Schizochytrium, biologically active fragments and homologues thereof, a nucleic acid sequence encoding such FAS, fragments and homologues thereof, the gene encoding Schizochytrium FAS, host cells and organisms that recombinantly express the FAS, host cells and organisms in which the expression and/or activity of the endogenous FAS has been attenuated, and various methods for making and using any of these proteins, nucleic acid molecules, genes, host cells or organisms.

5. 发明授权

US07118904B2 Nucleotide sequences which code for the eno gene 失效
公开(公告)号：US07118904B2
公开(公告)日：2006-10-10
申请号：US10728947
申请日：2003-12-08
申请人： Bettina Möckel , Walter Pfefferle , Thomas Hermann , Alfred Pühler , Jörn Kalinowski , Brigitte Bathe
发明人： Bettina Möckel , Walter Pfefferle , Thomas Hermann , Alfred Pühler , Jörn Kalinowski , Brigitte Bathe
IPC分类号： C12N1/20 , C12N9/00 , C12N9/24 , C12N1/22 , C07H2/04
CPC分类号： C12R1/15 , C12N9/88 , C12P13/08
摘要： The present invention is directed to nucleotide sequences coding for a bacterial enolase enzyme. These sequences may be used in improved methods for the fermentative preparation of amino acids using coryneform bacteria.

6. 发明授权

US5852183A Dog-mouse heterohybridoma and gene fragment coding for constant region of canine immunoglobulins 失效
标题翻译：犬小鼠异源杂交瘤和编码犬免疫球蛋白恒定区的基因片段
公开(公告)号：US5852183A
公开(公告)日：1998-12-22
申请号：US646981
申请日：1996-05-08
申请人： Hiroaki Maeda , Yasuyuki Eda , Kazuhiko Kimachi , Yoichi Ono , Sachio Tokiyoshi
发明人： Hiroaki Maeda , Yasuyuki Eda , Kazuhiko Kimachi , Yoichi Ono , Sachio Tokiyoshi
IPC分类号： A61K39/00 , C07K16/46 , C12N5/26 , C12N15/13 , C07H2/04 , C07H21/02
CPC分类号： C07K16/462 , A61K39/00 , C07K2317/24
摘要： A gene fragment which comprises a DNA sequence coding for an amino acid sequence of a constant region of canine immunoglobulin lambda chain; a gene fragment which comprises a DNA sequence coding for an amino acid sequence of a constant region of canine immunoglobulin kappa chain; a gene fragment which comprises a DNA sequence coding for the constant region of canine immunoglobulin gamma chain; a recombinant DNA molecule coding for an amino acid sequence of a mouse-dog chimeric antibody which comprises a gene fragment coding for an amino acid sequence of a variable region of a mouse immunoglobulin and a gene fragment coding for an amino acid sequence of a constant region of a canine immunoglobulin wherein the latter gene fragment; a polypeptide of a mouse-dog chimeric antibody which is expressed from a cell transformed with an expression vector for cells wherein said recombinant DNA molecule cording for an amino acid sequence of the mouse-dog chimeric antibody is incorporated; a dog-mouse heterohybridoma which produces canine immunoglobulin; and a process for preparing a canine immunoglobulin gene.
摘要翻译：一种基因片段，其包含编码犬免疫球蛋白λ链的恒定区的氨基酸序列的DNA序列; 基因片段，其包含编码犬免疫球蛋白κ链恒定区的氨基酸序列的DNA序列; 包含编码犬免疫球蛋白γ链的恒定区的DNA序列的基因片段; 编码小鼠 - 狗嵌合抗体的氨基酸序列的重组DNA分子，其包含编码小鼠免疫球蛋白的可变区的氨基酸序列的基因片段和编码恒定区的氨基酸序列的基因片段的犬免疫球蛋白，其中后者基因片段; 从用细胞表达载体转化的细胞中表达小鼠 - 狗嵌合抗体的多肽，其中并入所述针对小鼠 - 狗嵌合抗体的氨基酸序列的重组DNA分子; 产生犬免疫球蛋白的狗 - 小鼠异源杂交瘤; 以及制备犬免疫球蛋白基因的方法。

7. 发明授权

US07381555B2 Lactobacillus N-deoxyribosyl transferases, corresponding nucleotide sequences and their uses 失效
标题翻译：乳酸杆菌N-脱氧核糖基转移酶，相应的核苷酸序列及其用途
公开(公告)号：US07381555B2
公开(公告)日：2008-06-03
申请号：US11097292
申请日：2005-04-04
申请人： Pierre-Alexandre Kaminski , Patrick Tailliez , Philippe Marliere , Pascal Quenee , Rachel Cotaya
发明人： Pierre-Alexandre Kaminski , Patrick Tailliez , Philippe Marliere , Pascal Quenee , Rachel Cotaya
IPC分类号： C07H2/04 , C12N9/10 , C12N15/74 , C12N1/21
CPC分类号： C12P19/305 , C12N9/1077 , C12P19/32
摘要： The invention concerns novel polypeptides and their fragments, isolated from Lactobacillus, having at least a N-deoxyribosyl transferase activity, the polynucleotides encoding said polypeptides, cloning and/or expression vectors including said polynucleotides, cells transformed by said vectors and specific antibodies directed against said polypeptides. The invention also concerns a method for enzymatic synthesis of deoxyribonucleosides.
摘要翻译：本发明涉及从具有至少N-脱氧核糖基转移酶活性的乳杆菌分离的新型多肽及其片段，编码所述多肽的多核苷酸，包含所述多核苷酸的克隆和/或表达载体，由所述载体转化的细胞和针对所述多核苷酸的特异性抗体多肽。本发明还涉及一种酶合成脱氧核糖核苷的方法。

8. 发明授权

US06887467B1 Double mutants of dihydrofolate reductase and methods of using same 失效
标题翻译：二氢叶酸还原酶的双重突变体及其使用方法
公开(公告)号：US06887467B1
公开(公告)日：2005-05-03
申请号：US10650417
申请日：2003-08-27
申请人： Joseph R. Bertino , Emine A. Ercikan-Abali , Debabrata Banerjee , Shin Mineishi , Michel Sadelain
发明人： Joseph R. Bertino , Emine A. Ercikan-Abali , Debabrata Banerjee , Shin Mineishi , Michel Sadelain
IPC分类号： C12N9/06 , C12N15/65 , A61K38/44 , C07K1/00 , C12N1/20 , C12N9/10 , C07H2/04
CPC分类号： C12N9/003 , C12N15/65
摘要： New mutant forms of human dihydrofolate reductase (DHFR) which have properties superior to the previously disclosed mutants have mutations at both amino acid 22 and amino acid 31. Specific mutant forms are Ser31Tyr22, Ser31Phe22, Gly31Tyr22, Gly31Phe22, Ala31Tyr22 and Ala31Phe22. The mutant DHFR of the invention may be used as a selectable marker, and to modify the genome of human cells, particularly bone marrow cells or peripheral blood stem cells, to render them resistant to chemotherapy using antifolate agents.
摘要翻译：具有优于先前公开的突变体的性质的人二氢叶酸还原酶（DHFR）的新突变形式在氨基酸22和氨基酸31都具有突变。特异性突变体形式是Ser31Tyr22，Ser31Phe22，Gly31Tyr22，Gly31Phe22，Ala31Tyr22和Ala31Phe22。本发明的突变型DHFR可用作选择标记，并修饰人细胞，特别是骨髓细胞或外周血干细胞的基因组，以使其能够使用抗叶酸剂进行化学疗法。

9. 发明授权

US6022960A GnRH-leukotoxin chimeras 失效
标题翻译： GnRH-白细胞毒素嵌合体
公开(公告)号：US6022960A
公开(公告)日：2000-02-08
申请号：US124491
申请日：1998-07-29
申请人： Andrew A. Potter , John G. Manns
发明人： Andrew A. Potter , John G. Manns
IPC分类号： C12N15/09 , A61K39/00 , A61K39/39 , A61K47/48 , A61P15/18 , A61P35/00 , C07K5/103 , C07K5/11 , C07K5/113 , C07K5/117 , C07K7/23 , C07K14/14 , C07K14/285 , C07K14/575 , C07K14/655 , C07K16/26 , C07K19/00 , C12N1/21 , C12N15/16 , C12P21/02 , C12R1/19 , C07H2/04 , C12P21/06 , C07H2/02
CPC分类号： A61K39/0006 , A61K47/48238 , A61K47/4833 , A61K47/48484 , A61K47/485 , C07K14/005 , C07K14/285 , C07K14/655 , C07K16/26 , C07K5/1008 , C07K5/1019 , C07K5/1021 , C07K5/1024 , C07K7/23 , A61K2039/6037 , A61K39/00 , C07K2319/00 , C12N2720/12322
摘要： New immunological carrier systems, DNA encoding the same, and the use of these systems, are disclosed. The carrier systems include chimeric proteins which include a leukotoxin polypeptide fused to one or more selected GnRH multimers which comprise at least one repeating GnRH decapeptide sequence, or at least one repeating unit of a sequence corresponding to at least one epitope of a selected GnRH molecule. Under the invention, the selected GnRH sequences may all be the same, or may correspond to different derivatives, analogues, variants or epitopes of GnRH so long as the GnRH sequences are capable of eliciting an immune response. The leukotoxin functions to increase the immunogenicity of the GnRH multimers fused thereto.
摘要翻译：公开了新的免疫载体系统，编码相同的DNA以及这些系统的使用。载体系统包括嵌合蛋白，其包含与一个或多个选定的GnRH多聚体融合的白细胞毒素多肽，所述GnRH多聚体包含至少一个重复的GnRH十肽序列，或至少一个对应于所选GnRH分子的至少一个表位的序列的重复单元。根据本发明，所选择的GnRH序列可以全部相同，或者可以对应于GnRH的不同衍生物，类似物，变体或表位，只要GnRH序列能够引发免疫应答即可。白细胞毒素的作用是增加与其融合的GnRH多聚体的免疫原性。

10. 发明授权

US5969126A GNRH-leukotoxin chimeras 失效
标题翻译： GNRH-白细胞毒素嵌合体
公开(公告)号：US5969126A
公开(公告)日：1999-10-19
申请号：US878748
申请日：1997-06-19
申请人： Andrew A. Potter , John G. Manns
发明人： Andrew A. Potter , John G. Manns
IPC分类号： C12N15/09 , A61K38/00 , A61K39/00 , A61K47/48 , A61P43/00 , C07K5/103 , C07K5/11 , C07K5/113 , C07K5/117 , C07K7/23 , C07K14/14 , C07K14/285 , C07K14/655 , C07K16/26 , C07K19/00 , C12N1/21 , C12N15/00 , C12N15/31 , C12P21/02 , C12R1/19 , C12R1/91 , C07H2/04 , A61K39/02 , C12P21/06 , C07H2/02
CPC分类号： A61K47/48484 , A61K39/0006 , A61K47/48238 , A61K47/4833 , A61K47/485 , C07K14/005 , C07K14/285 , C07K14/655 , C07K16/26 , C07K5/1008 , C07K5/1019 , C07K5/1021 , C07K5/1024 , C07K7/23 , A61K2039/6037 , A61K39/00 , C07K2319/00 , C12N2720/12322
摘要： New immunological carrier systems, DNA encoding the same, and the use of these systems, are disclosed. The carrier systems include chimeric proteins which comprise a leukotoxin polypeptide fused to a selected GnRH multimer which consists essentially of at least one repeating GnRH decapeptide sequence, or at least one repeating unit of a sequence corresponding to at least one epitope of a selected GnRH molecule. Under the invention, the selected GnRH sequences may all be the same, or may correspond to different derivatives, analogues, variants or epitopes of GnRH so long as the GnRH sequences are capable of eliciting an immune response. The leukotoxin functions to increase the immunogenicity of the GnRH multimer fused thereto.
摘要翻译：公开了新的免疫载体系统，编码相同的DNA以及这些系统的使用。载体体系包括嵌合蛋白，其包含与选定的GnRH多聚体融合的白细胞毒素多肽，其基本上由至少一个重复的GnRH十肽序列组成，或至少一个对应于所选GnRH分子的至少一个表位的序列的重复单元。根据本发明，所选择的GnRH序列可以全部相同，或者可以对应于GnRH的不同衍生物，类似物，变体或表位，只要GnRH序列能够引发免疫应答即可。白细胞毒素的作用是增加与其融合的GnRH多聚体的免疫原性。

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