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1. 发明授权

US5204257A Method of recovering bacteriophage 失效
标题翻译：噬菌体回收方法
公开(公告)号：US5204257A
公开(公告)日：1993-04-20
申请号：US693133
申请日：1991-04-29
申请人： David A. DeBonville , Kelley A. Logan
发明人： David A. DeBonville , Kelley A. Logan
IPC分类号： C12N7/02
CPC分类号： C12N7/00 , C12N2795/14151 , Y10S530/821 , Y10S530/826
摘要： A method of recovering nucleic acid-containing particles from a liquid medium by contacting the liquid medium containing the particles with a mixture of hydroxylated silica beads and a salt solution to bind the nucleic acid-containing particles, centrifuging the mixture to pellet the bound particles, and separating the pellet from the liquid.
摘要翻译：通过使含有颗粒的液体介质与羟基化二氧化硅珠和盐溶液的混合物接触来结合含核酸颗粒的方法，从液体介质中回收含有核酸的颗粒的方法，将混合物离心以沉淀结合的颗粒，并将颗粒与液体分离。

2. 发明授权

US4341802A Production of protein with reduced nucleic acid 失效
标题翻译：用减少的核酸生产蛋白质
公开(公告)号：US4341802A
公开(公告)日：1982-07-27
申请号：US200367
申请日：1980-10-24
申请人： Thomas R. Hopkins
发明人： Thomas R. Hopkins
IPC分类号： A21D2/26 , A23J1/00 , C12N1/08 , C12N1/32 , C12P21/00 , A23L1/28
CPC分类号： A21D2/267 , A23J1/008 , C12N1/08 , C12N1/32 , Y10S435/804 , Y10S530/82 , Y10S530/821 , Y10S530/825
摘要： Microorganisms with low nucleic acid content are produced by acid treating of the microorganism. One embodiment relates to the extraction of so treated microorganisms after their separation from the acid treating fluid, using a strong base for the extraction. Another embodiment relates to the neutralization of the acid treating fluid containing nucleic acids prior to the introduction of this fluid into the fermentation zone, the anion of the acid and the metal cation used in the neutralization step forming minerals desirable for the growth medium in the fermentation step.
摘要翻译：具有低核酸含量的微生物通过对微生物进行酸处理而产生。一个实施方案涉及如此处理的微生物在与酸处理液分离后，使用强碱进行萃取。另一个实施方案涉及在将该流体引入发酵区之前，含有酸的酸处理液的中和，在中和步骤中使用的酸阴离子和金属阳离子形成发酵生长培养基所需的矿物质步。

3. 发明授权

US4228068A Peptide complexes of DNA-containing organisms 失效
标题翻译：含DNA生物的肽复合物
公开(公告)号：US4228068A
公开(公告)日：1980-10-14
申请号：US897549
申请日：1978-04-18
申请人： Gunter R. Wilhelm
发明人： Gunter R. Wilhelm
IPC分类号： A61K35/12 , A61K35/74 , A61K38/16 , A61K39/02 , A61P31/04 , A61P37/04 , G01N33/531 , C07G7/00 , A61K39/00
CPC分类号： G01N33/531 , A61K39/02 , A61K2039/6025 , A61K2039/622 , Y10S530/808 , Y10S530/809 , Y10S530/821 , Y10S530/822 , Y10S530/823 , Y10S530/824 , Y10S530/825 , Y10S530/826 , Y10S530/828 , Y10S530/829 , Y10S530/868
摘要： DNA peptide complexes are prepared from organisms containing DNA to produce specific peptide complexes in a substantially pure state and useful for diagnostic, therapeutic and prophylactic purposes. The complexes can be prepared from bacteria, fungi, plants, human or animal tissue by homogination, centrification, absorption on DEAE cellulose, serial elution of the cellulose followed by dialysis and collection.
摘要翻译：从含有DNA的生物体制备DNA肽复合物以产生基本上纯的状态的特异性肽复合物并且可用于诊断，治疗和预防目的。复合物可以由细菌，真菌，植物，人或动物组织通过均质化，离心，DEAE纤维素吸收，连续洗脱纤维素进行透析和收集。

4. 发明授权

US4079048A Process for preparing functional yeast proteins using alkaline conditions 失效
标题翻译：使用碱性条件制备功能酵母蛋白的方法
公开(公告)号：US4079048A
公开(公告)日：1978-03-14
申请号：US722050
申请日：1976-09-10
申请人： Kwei C. Chao
发明人： Kwei C. Chao
IPC分类号： A23J1/18 , C12N1/00 , C12N1/16 , C12P21/00
CPC分类号： C12N1/005 , A23J1/18 , Y10S530/821 , Y10S530/824
摘要： Functional yeast protein products with good thermogelability, emulsification capacity, foaming ability, solubility, and whippability are prepared by an alkali extraction of whole yeast cells preceded by a hot water extraction and/or a dilute alkaline extraction. These products are good substitutes for either casein, sodium caseinate, or egg white, and can also be used in making stabilized acidic protein beverages.
摘要翻译：通过在热水提取和/或稀碱提取之前的全酵母细胞的碱提取来制备具有良好的热粘合性，乳化能力，发泡能力，溶解性和可搅拌性的功能性酵母蛋白质产物。这些产品是酪蛋白，酪蛋白酸钠或蛋白的良好替代品，也可用于制备稳定的酸性蛋白质饮料。

5. 发明授权

US4021303A Method for treatment of microorganisms 失效
标题翻译：微生物处理方法
公开(公告)号：US4021303A
公开(公告)日：1977-05-03
申请号：US645505
申请日：1975-12-30
申请人： Yutaka Nakabayashi
发明人： Yutaka Nakabayashi
IPC分类号： A23J1/18 , A23J3/22 , C12N1/06 , A23J3/00
CPC分类号： A23J1/18 , A23J3/227 , C12N1/06 , Y10S435/804 , Y10S435/839 , Y10S435/843 , Y10S435/911 , Y10S435/921 , Y10S435/923 , Y10S435/924 , Y10S435/94 , Y10S435/941 , Y10S435/942 , Y10S435/946 , Y10S530/821 , Y10S530/824 , Y10S530/825
摘要： Microorganisms are chemically treated with alkali at a pH of 10.5 - 12.9 and a temperature of 0.degree. - 30.degree. C., washed with water and mechanically ruptured at a pH of 7 - 10.2.
摘要翻译：微生物用pH为10.5〜12.9，温度为0〜30℃的碱进行化学处理，用水洗涤，机械破碎，pH值为7〜10.2。

6. 发明授权

US3960659A Treatment of proteinaceous material 失效
标题翻译：蛋白质物质的处理
公开(公告)号：US3960659A
公开(公告)日：1976-06-01
申请号：US507716
申请日：1974-09-20
申请人： Stanley Fazakerley
发明人： Stanley Fazakerley
IPC分类号： C12P21/00 , A23J3/20 , C12N1/08 , C12B1/00
CPC分类号： C12N1/08 , A23J3/20 , Y10S435/923 , Y10S435/924 , Y10S530/821 , Y10S530/824
摘要： The nucleic acid content of proteinaceous material in particular hydrocarbon grown yeasts can be reduced by contact with extra-cellular ribonuclease, particularly by contact with the spent aqueous medium obtained after cultivating an extra cellular ribo-nuclease producing micro-organisms in a broth comprising a carbon substrate, an aqueous nutrient medium and a gas containing free oxygen.
摘要翻译：尤其是通过与细胞外核糖核酸酶的接触可以减少蛋白质物质的核酸含量，特别是通过在培养含有碳的肉汤培养细胞核糖核酸酶产生微生物之后获得的废水性培养基接触来减少底物，含水营养培养基和含游离氧的气体。

7. 发明授权

US3888839A Isolated yeast protein product with intact rna and a process for making same 失效
标题翻译：具有直肠RNA的分离的YEAST蛋白产品及其制备方法
公开(公告)号：US3888839A
公开(公告)日：1975-06-10
申请号：US31045572
申请日：1972-11-29
申请人： ANHEUSER BUSCH
发明人： NEWELL JON ALBERT , SEELEY ROBERT DUDLEY , ROBBINS ERNEST ALECK
IPC分类号： C12N1/00 , A23J1/18
CPC分类号： C12N1/005 , Y10S530/821 , Y10S530/824
摘要： This disclosure relates to a yeast protein isolate having its nucleic acid substantially intact. The composition of the protein is 65-85% protein, 9-14% nucleic acid, 2-8% ash, 9-14% lipid, 01% fiber and 2-10% carbohydrate. The process comprises rupturing the yeast cells, separating the cell walls from a soluble fraction containing the protein and precipitating the protein from the soluble fraction to recover a substantially cell free protein.

8. 发明申请

US20030092897A1 ANTIGEN COMPOSITION AGAINST MYCOPLASMA 失效
标题翻译：抗体组合物对MYCOPLASMA
公开(公告)号：US20030092897A1
公开(公告)日：2003-05-15
申请号：US08913430
申请日：1997-12-09
发明人： JOHN WALKER , ROGAN LEE , STEPHEN WILLIAM DOUGHTY
IPC分类号： C07H021/02 , C07H021/04
CPC分类号： C07K14/30 , A61K39/00 , C07K16/1253 , Y10S435/87 , Y10S530/806 , Y10S530/82 , Y10S530/821
摘要： The present invention relates to a putative protective antigen against a Mycoplasma, prepared by a method including a sample of a Mycoplasma; an antibody probe including at least one antibody against a Mycoplasma produced by a method including: providing a biological sample taken a short time after an immune animal has been challenged with a Mycoplasma or Mycoplasma extract taken from the infection site or an area of a lesion or an area close to the infection site or lesion; isolating cells from the biological sample; culturing cells in vitro in a suitable culture medium; and harvesting antibodies produced from said cells; probing the Mycoplasma sample with the antibody probe to detect at least one antigen; and isolating the antigen detected, also including diagnostic antigens, the preparation thereof, and their use in the formation of vaccine compositions, particularly vaccine compositions against Mycoplasma hyopneumoniae infections.
摘要翻译：本发明涉及通过包括支原体样品的方法制备的针对支原体的推定的保护性抗原; 一种抗体探针，其包含至少一种针对支原体的抗体，所述抗体通过以下方法产生，该方法包括：在免疫动物已用来自感染部位或病变区域的支原体或支原体提取物攻击之后提供短时间内的生物样品，或靠近感染部位或病变的区域; 从生物样品中分离细胞; 在合适的培养基中体外培养细胞; 并收获由所述细胞产生的抗体; 用抗体探针探测支原体样本以检测至少一种抗原; 并分离所检测的抗原，也包括诊断抗原，其制备及其在形成疫苗组合物，特别是针对猪肺炎支原体感染的疫苗组合物中的用途。

9. 发明授权

US5252328A Mycoplasma hyopneumoniae antigen and uses therefor 失效
标题翻译：猪肺炎支原体抗原及其用途
公开(公告)号：US5252328A
公开(公告)日：1993-10-12
申请号：US335726
申请日：1989-04-07
申请人： Daryl Faulds , Mimi Vishoot , Emily Brooks
发明人： Daryl Faulds , Mimi Vishoot , Emily Brooks
IPC分类号： A61K39/00 , C07K14/30 , A61K39/02
CPC分类号： C07K14/30 , A61K39/00 , Y10S424/825 , Y10S435/87 , Y10S530/806 , Y10S530/82 , Y10S530/821
摘要： A composition for protecting swine against mycoplasmal pneumonia caused by M. hyopneumoniae which includes at least one protein which is an M. hyopneumoniae antigen. The M. hyopneumoniae antigen is present in an amount effective for protection of swine against mycoplasmal pneumonia caused by M. hyopneumoniae. A preferred antigen is the M. hyopneumoniae 74.5 kda antigen.
摘要翻译：一种用于保护猪免受猪肺炎支原体引起的支原体肺炎的组合物，其包括至少一种是猪肺炎支原体抗原的蛋白质。猪肺炎支原体抗原以对猪肺炎支原体引起的针对支原体肺炎的保护有效的量存在。优选的抗原是猪肺炎支原体74.5kda抗原。

10. 发明授权

US4714613A Method of suppressing cell growth by immunotherapy 失效
标题翻译：免疫治疗抑制细胞生长的方法
公开(公告)号：US4714613A
公开(公告)日：1987-12-22
申请号：US428653
申请日：1982-09-30
申请人： Daniel Shouval , David A. Shafritz , Jack R. Wands
发明人： Daniel Shouval , David A. Shafritz , Jack R. Wands
IPC分类号： A61K38/00 , C07K16/08 , A61K39/42 , A61K39/00 , C12P21/00 , C12Q1/02
CPC分类号： C07K16/082 , A61K38/00 , C07K2317/73 , Y10S424/807 , Y10S530/821 , Y10S530/828 , Y10S530/864
摘要： A selective method of suppressing the growth of cells which express a viral antigen on the surface thereof, which comprises administering to the cells a growth suppressing amount of a monoclonal antibody against said viral antigen, especially a method of suppressing the growth of hepatocytes or hepatoma cells persistently infected with HBsAg which comprises administering to the cells a growth suppressing or lethal amount of a complement fixing monoclonal IgM or IgG.sub.2a antibody against HBsAg.
摘要翻译：抑制表面病毒抗原的细胞生长的选择性方法，其特征在于，对细胞施用生长抑制量的针对所述病毒抗原的单克隆抗体，特别是抑制肝细胞或肝癌细胞生长的方法持续感染HBsAg，其包括向细胞施用生长抑制或致死量的针对HBsAg的补体固定单克隆IgM或IgG2a抗体。

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