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    • 2. 发明申请
    • Trophic Conversion of Obligate Phototrophic Algae Through Metabolic Engineering
    • 通过代谢工程营养转化特种光营养藻类
    • US20120034653A1
    • 2012-02-09
    • US13188806
    • 2011-07-22
    • Kirk Emil APTF.C. Thomas AllnuttDavid J. KyleJames Casey Lippmeier
    • Kirk Emil APTF.C. Thomas AllnuttDavid J. KyleJames Casey Lippmeier
    • C12P21/00C12N15/79C12N1/13C07C29/74C11B1/10C07C35/21C07C7/00C07K14/405A23K1/00C07K1/14
    • C12N15/8209C12N1/12C12N15/65C12N15/79C12N15/8207Y10S435/946
    • Most microalgae are obligate photoautotrophs and their growth is strictly dependent on the generation of photosynthetically-derived energy. In this study it is shown that the microalga Phaeodaclylurn tricornutum can be engineered to import glucose and grow in the dark through the introduction of genes encoding glucose transporters. Both the human and Chlorella kessleri glucose transporters facilitated the uptake of glucose by P. tricornutum, allowing the cells to metabolize exogenous organic carbon and thrive, independent of light. This is the first successful trophic conversion of an obligate photoautotroph through metabolic engineering, and it demonstrates that methods of cell nourishment can be fundamentally altered with the introduction of a single gene. Since strains transformed with the glucose transport genes are able to grow non-photosynthetically, they can be exploited for the analysis of photosynthetic processes through mutant generation and characterization. Finally, this work also represents critical progress toward large-scale commercial exploitation of obligate phototrophic algae through the use of microbial fermentation technology, eliminating significant limitations resulting from light-dependent growth.
    • 大多数微藻是专性光自养体,其生长严格依赖于光合作用衍生能量的产生。 在这项研究中,显示微藻Phaeodaclylurn三角褐豆可以通过引入编码葡萄糖转运蛋白的基因进行工程化以导入葡萄糖并在黑暗中生长。 人和小球藻凯斯勒葡萄糖转运蛋白都促进了三角褐指藻的葡萄糖摄取,使得细胞代谢外源有机碳,并且独立于光。 这是通过代谢工程首次成功地营养专一性自养型营养转化,并且表明通过引入单一基因可以从根本上改变细胞营养的方法。 由于用葡萄糖转运基因转化的菌株能够非光合作用生长,因此可以通过突变体的产生和表征来开发光合作用的分析。 最后,这项工作也是通过使用微生物发酵技术大规模商业开发专用光营养藻类的重要进展,消除了光依赖性生长造成的重大限制。
    • 9. 发明申请
    • Procedure for obtaining a titrated preparation from algae and the resultant titrated preparations
    • 从藻类和所得滴定制剂中获得滴定制剂的方法
    • US20030086944A1
    • 2003-05-08
    • US09947898
    • 2001-09-06
    • Gutierrez Gilles
    • A61K035/80
    • C12Q1/025A61K36/03Y10S435/946
    • The present invention relates to the field of chemistry and more particularly to that of plant chemistry. Its specific aim is a process for obtaining powders or extracts from the alga Padina pavonica, titrated with active principles by means of a coefficient of equivalence with estradiol, which consists of dosing the extract or the powder of Padina pavonica using a method in which osteoblasts are cultivated in a medium rich in calcium ions, an analysis is made of the calcium fixed by the cells of the extracellular matrix in the presence or absence of agents antagonistic to the fixing of calcium, and the results are expressed either in arbitrary or international units in relation to a reference standard or by reference to the known activity of fixed doses of estradiol or of any other agent favouring the fixing of calcium.
    • 本发明涉及化学领域,更具体地涉及植物化学领域。 其具体目标是从藻类Padina pavonica获得粉末或提取物的方法,用活性成分通过与雌二醇相当的系数滴定,该方法包括使用成骨细胞的方法给予提取物或粉末的Padina pavonica 在富含钙离子的培养基中培养,在存在或不存在拮抗钙固定的药剂存在下,分析由细胞外基质的细胞固定的钙,结果以任意或国际单位表示 与参考标准的关系,或通过参考固定剂量的雌二醇或有利于固定钙的任何其他药剂的已知活性。