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1. 发明申请

US20080138851A1 TROPHIC CONVERSION OF OBLIGATE PHOTOTROPHIC ALGAE THROUGH METABOLIC ENGINEERING 失效
标题翻译：通过代谢工程进行的白藜芦醇的热转化
公开(公告)号：US20080138851A1
公开(公告)日：2008-06-12
申请号：US11842898
申请日：2007-08-21
申请人： Kirk Emil Apt , F.C. Thomas Allnutt , David J. Kyle , James Casey Lippmeier
发明人： Kirk Emil Apt , F.C. Thomas Allnutt , David J. Kyle , James Casey Lippmeier
IPC分类号： C12Q1/02 , C12N1/12 , C12N15/74
CPC分类号： C12N15/8209 , C12N1/12 , C12N15/65 , C12N15/79 , C12N15/8207 , Y10S435/946
摘要： Most microalgae are obligate photoautotrophs and their growth is strictly dependent on the generation of photosynthetically-derived energy. In this study it is shown that the microalga Phaeodaclylurn tricornutum can be engineered to import glucose and grow in the dark through the introduction of genes encoding glucose transporters. Both the human and Chlorella kessleri glucose transporters facilitated the uptake of glucose by P. tricornutum, allowing the cells to metabolize exogenous organic carbon and thrive, independent of light. This is the first successful trophic conversion of an obligate photoautotroph through metabolic engineering, and it demonstrates that methods of cell nourishment can be fundamentally altered with the introduction of a single gene. Since strains transformed with the glucose transport genes are able to grow non-photosynthetically, they can be exploited for the analysis of photosynthetic processes through mutant generation and characterization. Finally, this work also represents critical progress toward large-scale commercial exploitation of obligate phototrophic algae through the use of microbial fermentation technology, eliminating significant limitations resulting from light-dependent growth.
摘要翻译：大多数微藻是专性光自养体，其生长严格依赖于光合作用衍生能量的产生。在这项研究中，显示微藻Phaeodaclylurn三角褐豆可以通过引入编码葡萄糖转运蛋白的基因进行工程化以导入葡萄糖并在黑暗中生长。人和小球藻凯斯勒葡萄糖转运蛋白都促进了三角褐指藻的葡萄糖摄取，使得细胞代谢外源有机碳，并且独立于光。这是通过代谢工程首次成功地营养专一性自养型营养转化，并且表明通过引入单一基因可以从根本上改变细胞营养的方法。由于用葡萄糖转运基因转化的菌株能够非光合作用生长，因此可以通过突变体的产生和表征来开发光合作用的分析。最后，这项工作也是通过使用微生物发酵技术大规模商业开发专用光营养藻类的重要进展，消除了光依赖性生长造成的重大限制。

2. 发明申请

US20120034653A1 Trophic Conversion of Obligate Phototrophic Algae Through Metabolic Engineering 审中-公开
标题翻译：通过代谢工程营养转化特种光营养藻类
公开(公告)号：US20120034653A1
公开(公告)日：2012-02-09
申请号：US13188806
申请日：2011-07-22
申请人： Kirk Emil APT , F.C. Thomas Allnutt , David J. Kyle , James Casey Lippmeier
发明人： Kirk Emil APT , F.C. Thomas Allnutt , David J. Kyle , James Casey Lippmeier
IPC分类号： C12P21/00 , C12N15/79 , C12N1/13 , C07C29/74 , C11B1/10 , C07C35/21 , C07C7/00 , C07K14/405 , A23K1/00 , C07K1/14
CPC分类号： C12N15/8209 , C12N1/12 , C12N15/65 , C12N15/79 , C12N15/8207 , Y10S435/946
摘要： Most microalgae are obligate photoautotrophs and their growth is strictly dependent on the generation of photosynthetically-derived energy. In this study it is shown that the microalga Phaeodaclylurn tricornutum can be engineered to import glucose and grow in the dark through the introduction of genes encoding glucose transporters. Both the human and Chlorella kessleri glucose transporters facilitated the uptake of glucose by P. tricornutum, allowing the cells to metabolize exogenous organic carbon and thrive, independent of light. This is the first successful trophic conversion of an obligate photoautotroph through metabolic engineering, and it demonstrates that methods of cell nourishment can be fundamentally altered with the introduction of a single gene. Since strains transformed with the glucose transport genes are able to grow non-photosynthetically, they can be exploited for the analysis of photosynthetic processes through mutant generation and characterization. Finally, this work also represents critical progress toward large-scale commercial exploitation of obligate phototrophic algae through the use of microbial fermentation technology, eliminating significant limitations resulting from light-dependent growth.
摘要翻译：大多数微藻是专性光自养体，其生长严格依赖于光合作用衍生能量的产生。在这项研究中，显示微藻Phaeodaclylurn三角褐豆可以通过引入编码葡萄糖转运蛋白的基因进行工程化以导入葡萄糖并在黑暗中生长。人和小球藻凯斯勒葡萄糖转运蛋白都促进了三角褐指藻的葡萄糖摄取，使得细胞代谢外源有机碳，并且独立于光。这是通过代谢工程首次成功地营养专一性自养型营养转化，并且表明通过引入单一基因可以从根本上改变细胞营养的方法。由于用葡萄糖转运基因转化的菌株能够非光合作用生长，因此可以通过突变体的产生和表征来开发光合作用的分析。最后，这项工作也是通过使用微生物发酵技术大规模商业开发专用光营养藻类的重要进展，消除了光依赖性生长造成的重大限制。

3. 发明授权

US07939710B1 Trophic conversion of obligate phototrophic algae through metabolic engineering 失效
公开(公告)号：US07939710B1
公开(公告)日：2011-05-10
申请号：US09839536
申请日：2001-04-23
申请人： Kirk Emil Apt , F. C. Thomas Allnutt , David J. Kyle , James Casey Lippmeier
发明人： Kirk Emil Apt , F. C. Thomas Allnutt , David J. Kyle , James Casey Lippmeier
IPC分类号： C12N15/82
CPC分类号： C12N15/8209 , C12N1/12 , C12N15/65 , C12N15/79 , C12N15/8207 , Y10S435/946
摘要： Most microalgae are obligate photoautotrophs and their growth is strictly dependent on the generation of photosynthetically-derived energy. In this study it is shown that the microalga Phaeodaclylurn tricornutum can be engineered to import glucose and grow in the dark through the introduction of genes encoding glucose transporters. Both the human and Chlorella kessleri glucose transporters facilitated the uptake of glucose by P. tricornutum, allowing the cells to metabolize exogenous organic carbon and thrive, independent of light. This is the first successful trophic conversion of an obligate photoautotroph through metabolic engineering, and it demonstrates that methods of cell nourishment can be fundamentally altered with the introduction of a single gene. Since strains transformed with the glucose transport genes are able to grow non-photosynthetically, they can be exploited for the analysis of photosynthetic processes through mutant generation and characterization. Finally, this work also represents critical progress toward large-scale commercial exploitation of obligate phototrophic algae through the use of microbial fermentation technology, eliminating significant limitations resulting from light-dependent growth.

4. 发明申请

US20110195480A1 PRODUCTION OF HEMAGGLUTININ-NEURAMINIDASE PROTEIN IN MICROALGAE 有权
标题翻译：在微藻中生产HEMAGGLUTININ-NEURAMINIDASE蛋白
公开(公告)号：US20110195480A1
公开(公告)日：2011-08-11
申请号：US12980320
申请日：2010-12-28
申请人： Anne-Cécile V. Bayne , James Casey Lippmeier , Kirk Emil APT , Xuan Guo , Joyce A. Pritchard
发明人： Anne-Cécile V. Bayne , James Casey Lippmeier , Kirk Emil APT , Xuan Guo , Joyce A. Pritchard
IPC分类号： C12N9/26 , C12N1/13
CPC分类号： C12Y302/01018 , C12N7/00 , C12N9/2402 , C12N15/8257 , C12N2760/18151 , C12N2760/18651
摘要： The present invention is directed to recombinant microalgal cells and their use in production of heterologous hemagglutinin-neuraminidase (HN) polypeptides, as well as compositions and uses thereof.
摘要翻译：本发明涉及重组微藻细胞及其在产生异源血凝素 - 神经氨酸酶（HN）多肽中的用途，以及其组合物和用途。

5. 发明申请

US20110195449A1 Recombinant Thraustochytrids that Grow on Sucrose, and Compositions, Methods of Making, and Uses Thereof 审中-公开
标题翻译：在蔗糖上生长的重组Thraustochytrids及其组合物，制备方法及其用途
公开(公告)号：US20110195449A1
公开(公告)日：2011-08-11
申请号：US12980322
申请日：2010-12-28
申请人： James Casey Lippmeier , Kirk Emil Apt , Jon Milton Hansen
发明人： James Casey Lippmeier , Kirk Emil Apt , Jon Milton Hansen
IPC分类号： C12P1/00 , C12N15/63 , C12N1/13
CPC分类号： C12N15/79 , A23K20/10 , A23K50/80 , A23L2/52 , A23L33/10 , A23V2002/00 , C12N1/10 , C12N1/12 , C12N9/2405 , C12P7/6463 , C12P7/649 , C12Y302/01026 , C12Y302/01048 , Y02E50/13
摘要： The present invention is directed to recombinant thraustochytrids that grow on sucrose and cell cultures comprising the recombinant thraustochytrids as well as methods of producing cell cultures, biomasses, microbial oils, compositions, and biofuels using the recombinant thraustochytrids.
摘要翻译：本发明涉及在蔗糖上生长的重组thraustochytrid和包含重组thraustochytrids的细胞培养物，以及使用重组thraustochytrids生产细胞培养物，生物质，微生物油，组合物和生物燃料的方法。

6. 发明授权

US09012197B2 Production of hemagglutinin-neuraminidase protein in microalgae 有权
标题翻译：在微藻中生产血凝素 - 神经氨酸酶蛋白
公开(公告)号：US09012197B2
公开(公告)日：2015-04-21
申请号：US12980320
申请日：2010-12-28
申请人： Anne-Cécile V. Bayne , James Casey Lippmeier , Kirk Emil Apt , Xuan Guo , Joyce A. Pritchard
发明人： Anne-Cécile V. Bayne , James Casey Lippmeier , Kirk Emil Apt , Xuan Guo , Joyce A. Pritchard
IPC分类号： C12N9/26 , C12N9/14 , C12N1/12 , C12P21/06 , C07H21/04 , C07K1/00 , C12N7/00 , C12N15/82 , C12N9/24
CPC分类号： C12Y302/01018 , C12N7/00 , C12N9/2402 , C12N15/8257 , C12N2760/18151 , C12N2760/18651
摘要： The present invention is directed to recombinant microalgal cells and their use in production of heterologous hemagglutinin-neuraminidase (HN) polypeptides, as well as compositions and uses thereof.
摘要翻译：本发明涉及重组微藻细胞及其在产生异源血凝素 - 神经氨酸酶（HN）多肽中的用途，以及其组合物和用途。

7. 发明授权

US08637651B2 Protein production in microorganisms of the phylum labyrinthulomycota 有权
标题翻译：幽门螺杆菌菌门微生物中的蛋白质生产
公开(公告)号：US08637651B2
公开(公告)日：2014-01-28
申请号：US12724403
申请日：2010-03-15
申请人： Kirk E. Apt , James Casey Lippmeier , David Simpson , Jun Wang , James P. Wynn , Ross Zirkle
发明人： Kirk E. Apt , James Casey Lippmeier , David Simpson , Jun Wang , James P. Wynn , Ross Zirkle
IPC分类号： C07H21/04 , C12P21/02
CPC分类号： C12P21/00 , C07K14/37 , C07K14/705 , C12N9/2431 , C12N15/79 , C12N15/80 , C12P21/005 , C12R1/90 , C12Y302/01026
摘要： The present invention relates to recombinant cells and microorganisms of the phylum Labyrinthulomycota and their use in heterologous protein production. Novel promoter, terminator, and signal sequences for efficient production and, optionally, secretion of polypeptides from recombinant host cells and microorganisms are also encompassed by the present invention.
摘要翻译：本发明涉及神经胶霉病菌门的重组细胞和微生物及其在异源蛋白质生产中的用途。用于有效生产和任选地从重组宿主细胞和微生物分泌多肽的新型启动子，终止子和信号序列也包括在本发明中。

8. 发明申请

US20090064567A1 Biological oils and production and uses Thereof 审中-公开
标题翻译：生物油及其生产和用途
公开(公告)号：US20090064567A1
公开(公告)日：2009-03-12
申请号：US12230921
申请日：2008-09-08
申请人： James Casey Lippmeier , Joseph W. Pfeifer, III , Jon Milton Hansen , Kirk E. Apt , William Robert Barclay , Paul Warren Behrens , David Christian Martin
发明人： James Casey Lippmeier , Joseph W. Pfeifer, III , Jon Milton Hansen , Kirk E. Apt , William Robert Barclay , Paul Warren Behrens , David Christian Martin
IPC分类号： C10L1/18 , C12P7/64
CPC分类号： C12P7/6472 , C10L1/02 , C10L1/026 , C10L2200/0469 , C10L2270/026 , C10L2270/04 , C10L2290/26 , C12P7/649 , Y02E50/13 , Y02P30/20
摘要： The present invention provides biological oils and methods and uses thereof. The biological oils are preferably produced by heterotrophic fermentation of one or more microorganisms using cellulose-containing feedstock as a main source of carbon. The present invention also provides methods of producing lipid-based biofuels and food, nutritional, and pharmaceutical products using the biological oils.
摘要翻译：本发明提供生物油及其方法和用途。生物油优选通过使用含纤维素原料作为主要碳源的一种或多种微生物的异养发酵产生。本发明还提供了使用生物油制备基于脂质的生物燃料和食品，营养和药物产品的方法。

9. 发明授权

US07252979B2 Production of DHA in microalgae in low pH medium 有权
标题翻译：在低pH值培养基中生产微藻中的DHA
公开(公告)号：US07252979B2
公开(公告)日：2007-08-07
申请号：US11313524
申请日：2005-12-20
申请人： Paul W. Behrens , John M. Thompson , Kirk Apt , Joseph W. Pfeifer, III , James P. Wynn , James Casey Lippmeier , Jaouad Fichtali , Jon Hansen
发明人： Paul W. Behrens , John M. Thompson , Kirk Apt , Joseph W. Pfeifer, III , James P. Wynn , James Casey Lippmeier , Jaouad Fichtali , Jon Hansen
IPC分类号： C12P7/64 , C12N1/12 , A23D9/00 , A23L1/30 , C11B1/00
CPC分类号： C12P7/6427 , A61K36/02 , C11B1/10 , C12P7/6409 , C12P7/6472
摘要： Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.
摘要翻译：公开了使用低水平氯离子的海洋微生物生产高度不饱和脂肪酸的方法，包括异养海洋鞭毛藻Crypthecodinium。具体地说，通过操纵钠离子和钾离子水平，在低氯化物培养基中生长时，通过海洋微生物增加高度不饱和脂肪酸的生产的方法。本发明还涉及在低pH水平下由海洋生物生产高度不饱和脂肪酸的方法，并且包括产生低pH耐受菌株的方法。

10. 发明授权

US08426686B2 Polyunsaturated fatty acid production in heterologous organisms using PUFA polyketide synthase systems 有权
标题翻译：使用PUFA聚酮化合物系统在异源生物体中产生多不饱和脂肪酸
公开(公告)号：US08426686B2
公开(公告)日：2013-04-23
申请号：US12796100
申请日：2010-06-08
申请人： James G. Metz , Jerry M. Kuner , James Casey Lippmeier
发明人： James G. Metz , Jerry M. Kuner , James Casey Lippmeier
IPC分类号： A01H5/00 , A01H1/00 , C12N15/82 , C12N1/20 , C07H21/04
CPC分类号： C12N9/1029 , C12N9/93 , C12N15/8247 , C12P7/6427 , C12P7/6472 , Y02P20/52
摘要： Disclosed are novel acyl-CoA synthetases and novel acyltransferases, nucleic acid molecules encoding the same, recombinant nucleic acid molecules and recombinant host cells comprising such nucleic acid molecules, genetically modified organisms (microorganisms and plants) comprising the same, and methods of making and using the same. Also disclosed are genetically modified organisms (e.g., plants, microorganisms) that have been genetically modified to express a PKS-like system for the production of PUFAs (a PUFA PKS system or PUFA synthase), wherein the organisms have been modified to express an acyl-CoA synthetase, to express an acyl transferase, to delete or inactivate a fatty acid synthase (FAS) expressed by the organism, to reduce competition for malonyl CoA with the PUFA synthase or to increase the level of malonyl CoA in the organism, and in one aspect, to inhibit KASII or KASIII. Additional modifications, and methods to make and use such organisms, in addition to PUFAs and oils obtained from such organisms, are disclosed, alone with various products including such PUFAs and oils.
摘要翻译：公开了新型酰基辅酶A合成酶和新型酰基转移酶，编码该酰基转移酶的核酸分子，重组核酸分子和包含该核酸分子的重组宿主细胞，包含该核酸分子的转基因生物（微生物和植物），以及制备和使用一样。还公开了经遗传修饰以表达用于生产PUFA（PUFA PKS系统或PUFA合酶）的PKS样系统的遗传修饰生物体（例如，植物，微生物），其中生物体被修饰以表达酰基 -CoA合成酶，以表达酰基转移酶，以删除或失活由生物体表达的脂肪酸合酶（FAS），以减少与PUFA合酶的丙二酰辅酶A竞争或增加生物体中丙二酰辅酶A的水平，以及一方面抑制KASII或KASIII。除了PUFA和从这些生物获得的油之外，还公开了另外的修饰和制造和使用这些生物体的方法，其中包括各种产品，包括这种PUFA和油。

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