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1. 发明授权

US5834121A Composite magnetic beads 失效
标题翻译：复合磁珠
公开(公告)号：US5834121A
公开(公告)日：1998-11-10
申请号：US585905
申请日：1996-01-16
申请人： Irving Sucholeiki , Graham Margetts , Mark Roberts
发明人： Irving Sucholeiki , Graham Margetts , Mark Roberts
IPC分类号： B03C1/01 , C02F1/48 , C02F1/68 , G01N33/543 , H01F1/00 , H01F1/37 , B32B5/16 , B05D7/00
CPC分类号： B82Y25/00 , B03C1/01 , C02F1/681 , G01N33/5434 , H01F1/37 , C02F1/48 , G01N2446/20 , G01N2446/40 , G01N2446/86 , Y10S428/90 , Y10T428/2998
摘要： This invention provides a novel composite magnetic bead and a method for making the composite magnetic bead. The composite magnetic bead is comprised of a matrix formed from vinyl monomers throughout which is distributed primary beads, each primary bead of which is a magnetizable metal oxide encapsulated in a rigid polymeric coating. The matrix structure allows the composite magnetic bead to swell in organic solvents without loss of the intermeshed primary beads. Further, the matrix can be functionalized to allow the covalent bonding of sites useful for organic syntheses. The composite magnetic beads are made utilizing a core-shell polymerization techinque under conditions which preserve the oxidation state of the magnetizable metal oxide in the primary particles. In one embodiment, primary beads, each having a hydrophobic exterior surface, are dispersed in an inert solvent with a vinyl monomer and a crosslinking agent for that monomer to form a dispersed phase. The dispersed phase is mixed into an aqueous solvent-dispersing agent phase and stirred until the desired droplet size is achieved, then polymerization is initiated. After removal of the solvent and unreacted components, the composite magnetic beads are dried and sieved.
摘要翻译：本发明提供一种新型复合磁珠及其制造方法。复合磁珠由由乙烯基单体形成的基体组成，其中分布有初级珠，每个主珠是包封在刚性聚合物涂层中的可磁化金属氧化物。基体结构允许复合磁珠在有机溶剂中溶胀而不损失相互啮合的初级珠粒。此外，基质可以官能化以允许有机合成有用的位点的共价键。在保持一次颗粒中的可磁化金属氧化物的氧化态的条件下，使用核 - 壳聚合技术制备复合磁珠。在一个实施方案中，每个具有疏水性外表面的初级珠粒分散在具有乙烯基单体和用于该单体的交联剂的惰性溶剂中以形成分散相。将分散相混合到水性溶剂分散剂相中并搅拌直到达到所需的液滴尺寸，然后开始聚合。除去溶剂和未反应的组分后，将复合磁珠干燥并过筛。

2. 发明授权

US4929400A Production of monodisperse, polymeric microspheres 失效
标题翻译：单分散，聚合物微球的生产
公开(公告)号：US4929400A
公开(公告)日：1990-05-29
申请号：US856201
申请日：1986-04-28
申请人： Alan Rembaum , Won-Kyu Rhim , Michael T. Hyson , Manchium Chang
发明人： Alan Rembaum , Won-Kyu Rhim , Michael T. Hyson , Manchium Chang
IPC分类号： B03C1/01 , C08F2/46 , G01N15/14 , G01N33/543 , H01F1/06 , H01F1/11
CPC分类号： C08F2/46 , B03C1/01 , G01N33/54313 , G01N33/5434 , H01F1/061 , H01F1/112 , G01N2015/149 , G01N2030/484 , G01N2446/20 , G01N2446/40 , G01N2446/60
摘要： Very small, individual polymeric microspheres with very precise size and a wide variation in monomer type and properties are produced by deploying a precisely formed liquid monomer droplet, suitably an acrylic compound such as hydroxyethyl methacrylate into a containerless environment. The droplet which assumes a spheroid shape is subjected to polymerizing radiation such as ultraviolet or gamma radiation as it travels through the environment. Polymeric microspheres having precise diameters varying no more than plus or minus 5 percent from an average size are recovered. Many types of fillers including magnetic fillers may be dispersed in the liquid droplet.
摘要翻译：通过将精确形成的液体单体液滴，适当地将丙烯酸化合物如甲基丙烯酸羟乙酯部署到无容器的环境中，可以制备非常小的单个聚合物微球体，其尺寸非常精确和单体类型和性质的变化很大。呈现球状形状的液滴在穿过环境时经受聚合辐射如紫外或γ辐射。回收具有从平均尺寸变化不超过正或负5％的精确直径的聚合物微球。包括磁性填料的许多类型的填料可以分散在液滴中。

3. 发明授权

US4267234A Polyglutaraldehyde synthesis and protein bonding substrates 失效
标题翻译：聚戊二醛合成和蛋白质键合底物
公开(公告)号：US4267234A
公开(公告)日：1981-05-12
申请号：US21989
申请日：1979-03-19
申请人： Alan Rembaum
发明人： Alan Rembaum
IPC分类号： A61K9/50 , A61K39/44 , C07K14/805 , C08G6/00 , C12N11/08 , G01N33/531 , G01N33/543 , G01N33/58 , B32B5/16
CPC分类号： A61K39/44 , A61K9/50 , C07K14/805 , C08G6/00 , C12N11/08 , G01N33/531 , G01N33/5434 , G01N33/582 , G01N33/585 , G01N2446/20 , G01N2446/30 , G01N2446/40 , G01N2446/60 , Y10S428/90 , Y10S428/913 , Y10S436/80 , Y10T428/2991 , Y10T428/2996 , Y10T428/2998 , Y10T428/31942
摘要： Polyglutaraldehyde (PGL) is polymerized in aqueous base or in aqueous highly polar solvent basic media to prepare powders, castable films or coatings for substrates such as amine substituted microbeads. PGL microspheres can be prepared by suspension polymerization in presence of a surfactant or by precipitating PGL from solution containing surfactant. Magnetic PGL microspheres are formed by suspension polymerization in the presence of magnetic particles such as iron oxide. Polyglutaraldehyde can be converted to a fluorescent polymer by reaction with m-aminophenol or other reagent. Proteins can be readily covalently bound to the polyglutaraldehyde.
摘要翻译：聚戊二醛（PGL）在碱性水溶液或高极性极性溶剂碱性介质中进行聚合，以制备用于底物例如胺取代的微珠的粉末，浇铸膜或涂层。 PGL微球可以通过在表面活性剂存在下的悬浮聚合或通过从含有表面活性剂的溶液中沉淀PGL来制备。磁性PGL微球通过悬浮聚合在磁性颗粒如氧化铁存在下形成。聚戊二醛可以通过与间氨基苯酚或其他试剂反应而转化为荧光聚合物。蛋白质可以容易地与聚戊二醛共价结合。

4. 发明申请

US20050065288A1 Process for producing particles for diagnostic reagent 有权
标题翻译：用于制备诊断试剂的颗粒的方法
公开(公告)号：US20050065288A1
公开(公告)日：2005-03-24
申请号：US10940750
申请日：2004-09-15
申请人： Ichirou Ozaki , Satoshi Katayose , Mikio Hikata , Kejun Fan , Mitsuhiro Murata
发明人： Ichirou Ozaki , Satoshi Katayose , Mikio Hikata , Kejun Fan , Mitsuhiro Murata
IPC分类号： C08F2/44 , G01N33/543 , C08L53/00
CPC分类号： C08F2/44 , G01N33/5434 , G01N2446/40
摘要： A process of producing particles for diagnostic reagent, including forming a matrix particle by mixing a nuclear particle having a CV value of 20% or less and a magnetic substance containing at least one of Fe2O3 and Fe3O4 and giving a shear force to the mixture to adsorb the magnetic substance on the surface of the nuclear particles; and polymerizing a polymerizable monomer in the presence of said matrix particle to form a polymer layer on the magnetic substance layer.
摘要翻译：制造用于诊断试剂的颗粒的方法，包括通过混合CV值为20％以下的核粒子和含有Fe 2 O 3和Fe 3 O 4中的至少一种的磁性物质形成基质颗粒，并向该混合物吸附而产生剪切力核粒子表面的磁性物质; 并在所述基体颗粒的存在下聚合可聚合单体以在磁性物质层上形成聚合物层。

5. 发明授权

US06013531A Method to use fluorescent magnetic polymer particles as markers in an immunoassay 失效
标题翻译：荧光磁性聚合物颗粒作为免疫测定中的标记物的方法
公开(公告)号：US06013531A
公开(公告)日：2000-01-11
申请号：US518135
申请日：1995-08-22
申请人： Chaeo-Huej J. Wang , Dinesh O. Shah
发明人： Chaeo-Huej J. Wang , Dinesh O. Shah
IPC分类号： C08F257/02 , C08F285/00 , C12Q1/68 , G01N33/543 , G01N33/58 , G01N33/553 , G01N33/546
CPC分类号： C12Q1/6834 , C08F257/02 , C08F285/00 , G01N33/54326 , G01N33/582 , G01N33/585 , G01N2446/10 , G01N2446/40 , G01N2446/84 , G01N2446/86 , Y10S435/967
摘要： This invention provides a novel process of using magnetically responsive fluorescent polymer particles comprising polymeric core particles coated evenly with a layer of polymer containing magnetically responsive metal oxide as highly sensitive quantitative reagents for biochemical and immunological studies, wherein the fluorescent magnetic particles can serve as a marker for the number of particles. A wide variety of polymeric particles with sizes ranging from 1 to 100 microns can be used a core particles and transformed into magnetically responsive polymer particles. The surface of these magnetically responsive polymer particles can be coated further with another layer of functionalized polymer. These magnetically responsive fluorescent polymer particles can be used for passive or covalent coupling of biological material such as antigens, antibodies, enzymes or DNA/RNA hybridization and used as solid phase for various types of immunoassays, DNA/RNA hybridization probes assays, affinity purification, cell separation and other medical, diagnostic, and industrial applications.
摘要翻译：本发明提供了一种使用磁响应荧光聚合物颗粒的新方法，其包括均匀包被含有磁响应金属氧化物的聚合物层的聚合物芯颗粒，作为生物化学和免疫学研究的高灵敏度定量试剂，其中荧光磁性颗粒可以作为标记对于颗粒数量。尺寸范围为1至100微米的多种聚合物颗粒可用于核心颗粒并转化为磁响应聚合物颗粒。这些磁响应聚合物颗粒的表面可以进一步被另一层官能化聚合物涂覆。这些磁响应荧光聚合物颗粒可用于生物材料如抗原，抗体，酶或DNA / RNA杂交的被动或共价偶联，并用作各种类型的免疫测定的固相，DNA / RNA杂交探针测定，亲和纯化，细胞分离等医疗，诊断和工业应用。

6. 发明授权

US4552812A Process for the production of polyacrolein microspheres and grafted microspheres 失效
标题翻译：制备聚丙烯醛微球和接枝微球的方法
公开(公告)号：US4552812A
公开(公告)日：1985-11-12
申请号：US330451
申请日：1981-12-14
申请人： Shlomo Margel , Uzi Beitler
发明人： Shlomo Margel , Uzi Beitler
IPC分类号： A61K9/50 , A61K9/51 , A61K9/58 , A61K39/44 , A61K47/48 , C08F2/22 , C08F2/24 , C08F16/34 , C12N11/08 , G01N33/543 , B32B5/16 , B01J13/02
CPC分类号： A61K9/5138 , A61K39/44 , A61K47/48176 , A61K9/5094 , C08F16/34 , C12N11/08 , G01N33/54313 , G01N33/5434 , G01N2446/20 , G01N2446/30 , G01N2446/40 , G01N2446/86 , Y10S526/909 , Y10T428/2989 , Y10T428/2998 , Y10T436/25375
摘要： Suspension polymerization of acrolein type compounds in the presence of appropriate surfactants results in the formation of microspheres in size ranging from 0.03.mu. to 80.mu.. Fluorescent and magnetic microspheres are obtained by carrying out the same polymerization in the presence of appropriate fluorochromic or ferrofluidic compounds, respectively. Hybrido polyacrolein microspheres are obtained by grafting one type of such microspheres on another type. Immunomicrospheres were formed by binding covalently at physiological pH appropriate proteins to the microspheres. The immunomicrospheres can be used for various biological applications, such as specific markers for labelling cell surface receptors, for cell separation, for diagnostic purposes, etc.
摘要翻译：在适当的表面活性剂存在下丙烯醛型化合物的悬浮聚合导致尺寸范围为0.03至80微米的微球的形成。荧光和磁性微球分别通过在适当的荧光色素或铁磁性化合物存在下进行相同的聚合而获得。通过将一种类型的这种微球接枝在另一种类型上来获得杂环聚丙烯醛微球。通过在生理pH适当的蛋白质上共价结合到微球体上形成免疫微球。免疫微球可用于各种生物应用，例如用于标记细胞表面受体的特异性标记，用于细胞分离，用于诊断目的等。

7. 发明授权

US06797782B2 Process for producing particles for diagnostic reagent 有权
标题翻译：用于制备诊断试剂的颗粒的方法
公开(公告)号：US06797782B2
公开(公告)日：2004-09-28
申请号：US10392891
申请日：2003-03-21
申请人： Ichiro Ozaki , Satoshi Katayose , Mikio Hikata , Kejun Fan , Mitsuhiro Murata
发明人： Ichiro Ozaki , Satoshi Katayose , Mikio Hikata , Kejun Fan , Mitsuhiro Murata
IPC分类号： C08F22210
CPC分类号： C08F2/44 , G01N33/5434 , G01N2446/40
摘要： A process of producing particles for diagnostic reagent, including a step of polymerizing a polymerizable monomer in the presence of a matrix particle having a magnetic substance layer containing at least one of Fe2O3 and Fe3O4 on the surface thereof and having a mean particle size of 0.4-200 &mgr;m to form a polymer layer on the magnetic substance layer.
摘要翻译：一种生产用于诊断试剂的颗粒的方法，包括在其表面上具有含有至少一种Fe 2 O 3和Fe 3 O 4的磁性物质层的基质颗粒的存在下聚合可聚合单体并具有0.4〜 200微米，以在磁性物质层上形成聚合物层。

8. 发明授权

US6133047A Superparamagnetic monodisperse particles 失效
标题翻译：超顺磁单分散颗粒
公开(公告)号：US6133047A
公开(公告)日：2000-10-17
申请号：US983040
申请日：1998-01-15
申请人： Abdelhamid Elaissari , Christian Pichot , Bernard Mandrand , Florence Sauzedde
发明人： Abdelhamid Elaissari , Christian Pichot , Bernard Mandrand , Florence Sauzedde
IPC分类号： B03C1/01 , G01N33/537 , G01N33/543 , H01F1/37 , H01F1/44 , G01N33/553 , B05D5/12 , B32B5/16 , G01N33/566
CPC分类号： H01F1/37 , B03C1/01 , G01N33/5375 , G01N33/5434 , H01F1/44 , G01N2446/10 , G01N2446/40 , G01N2446/84 , G01N2446/86 , Y10S436/806 , Y10S436/824 , Y10T428/2982 , Y10T428/2989 , Y10T428/2991 , Y10T428/2998 , Y10T436/24
摘要： The superparamagnetic monodispersed particles comprise a core of a first polymer, an internal layer of a second polymer coating the core and in which a magnetic material is distributed, and an external layer of a third polymer coating the magnetic layer and capable of interacting with at least one biological molecule. At least the second polymer is heat sensitive and has a predetermined lower critical solubility temperature (LCST) of 15-65.degree. C. These particles may be used to isolate at least one biological molecule from a liquid specimen.
摘要翻译： PCT No.PCT / FR97 / 00912 Sec。 371日期1998年1月15日 102（e）日期1998年1月15日PCT提交1997年5月23日PCT公布。出版物WO97 / 45202 日本1997年12月4日超顺磁单分散颗粒包括第一聚合物的芯，涂覆芯的第二聚合物的内层，其中分布有磁性材料，以及涂覆磁性层的第三聚合物的外层，与至少一种生物分子相互作用。至少第二聚合物是热敏感的并且具有15-65℃的预定的较低临界溶解温度（LCST）。这些颗粒可用于从液体样品中分离至少一种生物分子。

9. 发明授权

US5395688A Magnetically responsive fluorescent polymer particles 失效
标题翻译：磁响应荧光聚合物颗粒
公开(公告)号：US5395688A
公开(公告)日：1995-03-07
申请号：US114299
申请日：1993-08-30
申请人： Chao-Huei J. Wang , Dinesh Q. Shah
发明人： Chao-Huei J. Wang , Dinesh Q. Shah
IPC分类号： A61M1/36 , B01J8/00 , B01J20/28 , B03C1/01 , C08F257/02 , C08F285/00 , C12Q1/68 , G01N33/543 , G01N33/58 , H01F1/11 , B32B5/16 , G01N33/553
CPC分类号： B01J20/28016 , A61M1/3618 , B01J20/28004 , B01J20/28009 , B01J8/008 , B03C1/01 , C08F257/02 , C08F285/00 , C12Q1/6816 , C12Q1/6834 , G01N33/54326 , G01N33/5434 , G01N33/582 , G01N33/585 , H01F1/111 , G01N2446/10 , G01N2446/40 , G01N2446/84 , G01N2446/86 , Y10S525/902 , Y10T428/25 , Y10T428/254 , Y10T428/257 , Y10T428/266 , Y10T428/2991 , Y10T428/2998
摘要： This invention provides a novel process of producing magnetically responsive fluorescent polymer particles comprising polymeric core particles coated evenly with a layer of polymer containing magnetically responsive metal oxide. A wide variety of polymeric particles with sizes ranging from 1 to 100 microns can be used a core particles and transformed into magnetically responsive polymer particles. The surface of these magnetically responsive polymer particles can be coated further with another layer of functionalized polymer. These magnetically responsive fluorescent polymer particles can be used for passive or covalent coupling of biological material such as antigens, antibodies, enzymes or DNA/RNA hybridization and used as solid phase for various types of immunoassays, DNA/RNA hybridization probes assays, affinity purification, cell separation and other medical, diagnostic, and industrial applications.
摘要翻译：本发明提供了一种制备磁响应荧光聚合物颗粒的新方法，其包括均匀地涂覆有含有磁响应金属氧化物的聚合物层的聚合物芯颗粒。尺寸范围为1至100微米的多种聚合物颗粒可用于核心颗粒并转化为磁响应聚合物颗粒。这些磁响应聚合物颗粒的表面可以进一步被另一层官能化聚合物涂覆。这些磁响应荧光聚合物颗粒可用于生物材料如抗原，抗体，酶或DNA / RNA杂交的被动或共价偶联，并用作各种类型的免疫测定的固相，DNA / RNA杂交探针测定，亲和纯化，细胞分离等医疗，诊断和工业应用。

10. 发明授权

US4369226A Polyglutaraldehyde synthesis and protein bonding substrates 失效
标题翻译：聚戊二醛合成和蛋白质键合底物
公开(公告)号：US4369226A
公开(公告)日：1983-01-18
申请号：US235043
申请日：1981-02-17
申请人： Alan Rembaum
发明人： Alan Rembaum
IPC分类号： A61K9/50 , A61K39/44 , C07K14/805 , C08G6/00 , C12N11/08 , G01N33/543 , G01N33/58 , B32B5/16 , B32B9/00
CPC分类号： G01N33/585 , A61K39/44 , A61K9/50 , C07K14/805 , C08G6/00 , C12N11/08 , G01N33/54313 , G01N33/5434 , G01N2446/20 , G01N2446/30 , G01N2446/40 , G01N2446/60 , Y10T428/263 , Y10T428/2996 , Y10T428/2998 , Y10T428/31942
摘要： Polyglutaraldehyde (PGL) is polymerized in aqueous base or in aqueous highly polar solvent basic media to prepare powders, castable films or coatings for substrates such as amine substituted microbeads. PGL microspheres can be prepared by suspension polymerization in presence of a surfactant or by precipitating PGL from solution containing surfactant. Magnetic PGL microspheres are formed by suspension polymerization in the presence of magnetic particles such as iron oxide. Polyglutaraldehyde can be converted to a fluorescent polymer by reaction with m-aminophenol or other reagent. Proteins can be readily covalently bound to the polyglutaraldehyde.
摘要翻译：聚戊二醛（PGL）在碱性水溶液或高极性极性溶剂碱性介质中进行聚合，以制备用于底物例如胺取代的微珠的粉末，浇铸膜或涂层。可以通过在表面活性剂存在下的悬浮聚合或通过从含有表面活性剂的溶液中沉淀PGL来制备PGL微球。磁性PGL微球通过悬浮聚合在磁性颗粒如氧化铁存在下形成。聚戊二醛可以通过与间氨基苯酚或其他试剂反应而转化为荧光聚合物。蛋白质可以容易地与聚戊二醛共价结合。

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