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1. 发明申请

US20170369869A1 EXPRESSION AND SECRETION SYSTEM 审中-公开
公开(公告)号：US20170369869A1
公开(公告)日：2017-12-28
申请号：US15690544
申请日：2017-08-30
申请人： Genentech, Inc.
发明人： Devin TESAR , Xiaocheng CHEN , Mark DENNIS , Isidro HOTZEL
IPC分类号： C12N15/10 , C12N15/74 , C12P21/02 , C12N15/70 , C12N15/85 , C07K16/00
CPC分类号： C12N15/1037 , C07K16/00 , C07K2319/02 , C07K2319/40 , C07K2319/735 , C12N15/70 , C12N15/74 , C12N15/85 , C12N2820/10 , C12N2820/55 , C12N2820/85 , C12N2830/42 , C12N2830/55 , C12N2830/85 , C12P21/02
摘要： The invention provides an expression and secretion system, and methods of using the same, for the expression and secretion of one fusion protein in prokaryotic cells and a second fusion protein in eukaryotic cells. Also provided herein are nucleic acid molecules, vectors and host cells comprising such vectors and nucleic acid molecules.

2. 发明申请

US20110143423A1 Viral vectors whose replication and, optionally, passenger gene are controlled by a gene switch activated by heat in the presence or absence of a small-molecule regulator 有权
标题翻译：在存在或不存在小分子调节剂的情况下，其复制和任选的乘客基因由热激活的基因开关控制的病毒载体
公开(公告)号：US20110143423A1
公开(公告)日：2011-06-16
申请号：US12929553
申请日：2011-02-01
申请人： Richard Voellmy
发明人： Richard Voellmy
IPC分类号： C12N7/00
CPC分类号： A61K48/0066 , A61K35/761 , C12N7/00 , C12N15/63 , C12N15/85 , C12N15/86 , C12N2710/10332 , C12N2710/10343 , C12N2800/40 , C12N2820/002 , C12N2820/85 , C12N2830/00 , C12N2830/001 , C12N2830/002 , C12N2830/15 , C12N2830/85 , C12N2840/203
摘要： The present invention relates to conditionally replicating viruses or pairs of viruses containing a gene switch that is activatable by transient heat or other proteotoxic stress in the presence or absence of a small molecule regulator. The gene switch controls the expression of a gene for a protein required for efficient viral replication and may also control the activity of a passenger gene.
摘要翻译：本发明涉及有条件地复制病毒或病毒对，其含有在存在或不存在小分子调节剂的情况下可通过瞬时热或其他蛋白毒性应激激活的基因开关。基因开关控制用于有效病毒复制所需的蛋白质的基因的表达，并且还可以控制乘客基因的活性。

3. 发明公开

US20240093182A1 EUKARYOTIC DNA REPLICATION ORIGINS, AND VECTOR CONTAINING THE SAME 审中-公开
公开(公告)号：US20240093182A1
公开(公告)日：2024-03-21
申请号：US18041902
申请日：2021-09-06
申请人： CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE , UNIVERSITÉ DE MONTPELLIER
发明人： Marcel MECHALI , Ildem AKERMAN , Nadège GABORIT
IPC分类号： C12N15/10 , C12N15/85
CPC分类号： C12N15/1093 , C12N15/85 , C12N2820/85
摘要： A method for isolating a mammalian genomic DNA replication origin, the method including: isolating the genomic DNA molecules; identifying 500 bp windows within the DNA molecules; isolating from the genomic DNA molecules the fragments that have a size from 500 pb up 6000 pb; selecting a DNA replication origin that is able, when contained in the DNA of an Eukaryotic cell, to produce nascent DNA, and to initiate DNA replication; and isolating the origin.

4. 发明授权

US09803191B2 Expression and secretion system 有权
公开(公告)号：US09803191B2
公开(公告)日：2017-10-31
申请号：US13934570
申请日：2013-07-03
申请人： Genentech, Inc.
发明人： Devin Tesar , Xiaocheng Chen , Mark Dennis , Isidro Hotzel
IPC分类号： C12N15/10 , C12N15/74 , C12N15/70 , C07K16/00 , C12N15/85 , C12P21/02
CPC分类号： C12N15/1037 , C07K16/00 , C07K2319/02 , C07K2319/40 , C07K2319/735 , C12N15/70 , C12N15/74 , C12N15/85 , C12N2820/10 , C12N2820/55 , C12N2820/85 , C12N2830/42 , C12N2830/55 , C12N2830/85 , C12P21/02
摘要： The invention provides an expression and secretion system, and methods of using the same, for the expression and secretion of one fusion protein in prokaryotic cells and a second fusion protein in eukaryotic cells. Also provided herein are nucleic acid molecules, vectors and host cells comprising such vectors and nucleic acid molecules.

5. 发明授权

US08771983B2 Method and kit for expressing protein under regulation of the expression from repeated sequence formed by gene amplification, and transformant 有权
标题翻译：用于通过基因扩增形成的重复序列表达调控表达蛋白质的方法和试剂盒，以及转化体
公开(公告)号：US08771983B2
公开(公告)日：2014-07-08
申请号：US11889512
申请日：2007-08-14
申请人： Noriaki Shimizu
发明人： Noriaki Shimizu
IPC分类号： C12N15/85 , C12N15/87 , C12N15/90 , C12N15/113 , C12N5/16
CPC分类号： C12N15/67 , C12N15/85 , C12N2810/85 , C12N2820/85 , C12P21/02
摘要： A method is disclosed for releasing the transcriptional regulation caused by a repeated sequence in a gene, a kit therefor and so on to thereby establish a system capable of producing a protein in a large amount. At least one embodiment of the method can be achieved by any one or more of the following methods: (a) in the amplification of a gene encoding a target protein, co-amplifying a polynucleotide of 10 kbp or more such as a λ-phage DNA or an insulator sequence; (b) selecting by culturing cells having undergone gene amplification in media containing a drug with a gradual increase in concentration; (c) elevating the promoter activity of inducing the expression of a gene encoding a target protein; (d) excising an amplified gene region from a chromosome with the use of Cre-LoxP System; (e) treating cells having undergone gene amplification with 5-aza-2′-deoxycytidine to thereby lower the methylation degree of DNA; and (f) selecting the mammalian cells having undergone gene amplification on double minute chromosomes.
摘要翻译：公开了一种用于释放由基因中的重复序列引起的转录调控的方法，其用于其的试剂盒等，从而建立能够大量产生蛋白质的系统。该方法的至少一个实施方案可以通过以下任何一种或多种方法来实现：（a）扩增编码靶蛋白的基因，共扩增10kbp以上的多核苷酸，例如λ噬菌体 DNA或绝缘子序列; （b）通过培养在具有逐渐增加浓度的药物的培养基中培养经历基因扩增的细胞进行选择; （c）提高诱导编码靶蛋白的基因表达的启动子活性; （d）使用Cre-LoxP系统从染色体切除扩增的基因区域; （e）处理已经用5-氮杂-2'-脱氧胞苷进行基因扩增的细胞，从而降低DNA的甲基化程度; 和（f）选择在双分子染色体上经历基因扩增的哺乳动物细胞。

6. 发明申请

US20140011981A1 EXPRESSION AND SECRETION SYSTEM 审中-公开
标题翻译：表达和分泌系统
公开(公告)号：US20140011981A1
公开(公告)日：2014-01-09
申请号：US13934570
申请日：2013-07-03
申请人： Genentech, Inc.
发明人： Devin Tesar , Xiaocheng Chen , Mark Dennis , Isidro Hotzel
IPC分类号： C12N15/10 , C07K16/00 , C12P21/02 , C12N15/70 , C12N15/85
CPC分类号： C12N15/1037 , C07K16/00 , C07K2319/02 , C07K2319/40 , C07K2319/735 , C12N15/70 , C12N15/74 , C12N15/85 , C12N2820/10 , C12N2820/55 , C12N2820/85 , C12N2830/42 , C12N2830/55 , C12N2830/85 , C12P21/02
摘要： The invention provides an expression and secretion system, and methods of using the same, for the expression and secretion of one fusion protein in prokaryotic cells and a second fusion protein in eukaryotic cells. Also provided herein are nucleic acid molecules, vectors and host cells comprising such vectors and nucleic acid molecules.
摘要翻译：本发明提供了表达和分泌系统及其使用方法，用于在真核细胞中的原核细胞和第二融合蛋白中表达和分泌一种融合蛋白。本文还提供了包含这种载体和核酸分子的核酸分子，载体和宿主细胞。

7. 发明申请

US20100173281A1 HCV NS3 protease replicon shuttle vectors 审中-公开
标题翻译： HCV NS3蛋白复制子穿梭载体
公开(公告)号：US20100173281A1
公开(公告)日：2010-07-08
申请号：US12319387
申请日：2009-01-06
申请人： Samir Ali , Wen-Rong Jiang
发明人： Samir Ali , Wen-Rong Jiang
IPC分类号： C12Q1/70 , C12N15/63
CPC分类号： C12Q1/706 , C12N7/00 , C12N2770/24222 , C12N2770/24243 , C12N2770/24262 , C12N2820/10 , C12N2820/55 , C12N2820/85 , C12N2840/206 , C12N2840/60
摘要： The present invention provides for novel HCV NS3 protease replicon shuttle vectors useful for cloning in HCV polynucleotide sequences from samples of HCV-infected patients and testing the resulting replicons for drug susceptibility.
摘要翻译：本发明提供了用于克隆HCV感染患者样品的HCV多核苷酸序列的新型HCV NS3蛋白复制子穿梭载体，并测试了所得复制子的药物敏感性。

8. 发明申请

US20030166140A1 COMPOSITIONS AND METHODS FOR IDENTIFYING ANTIGENS WHICH ELICIT AN IMMUNE RESPONSE 失效
标题翻译：用于鉴定免疫应答的抗原的组合物和方法
公开(公告)号：US20030166140A1
公开(公告)日：2003-09-04
申请号：US10201764
申请日：2002-07-22
申请人： Wake Forest University
发明人： Si-Yi Chen , Zhaoyang You
IPC分类号： C12P021/06 , C12P019/34 , C12N015/00 , C12N015/09 , C12N015/63 , C12N015/70 , C12N015/74 , C12N005/08
CPC分类号： C12N15/1086 , A61K38/00 , A61K39/00 , A61K39/0011 , A61K39/12 , A61K39/292 , A61K47/646 , A61K48/00 , A61K2039/515 , A61K2039/5154 , A61K2039/5156 , A61K2039/53 , A61K2039/55533 , A61K2039/57 , A61K2039/6056 , C07K14/005 , C07K2319/02 , C07K2319/30 , C07K2319/74 , C12N15/86 , C12N2730/10122 , C12N2730/10134 , C12N2740/10043 , C12N2740/10051 , C12N2740/13043 , C12N2740/13051 , C12N2740/15043 , C12N2740/15051 , C12N2820/85 , C12N2840/20 , C12N2840/203 , C12Q1/6883 , C12Q1/6886 , C12Q1/70
摘要： This invention relates to an expression vector wherein said expression vector comprises a polynucleotide promoter sequence, a polynucleotide encoding a signal sequence, a polynucleotide encoding an antigen protein or peptide, a polynucleotide encoding a cell binding element, and a polynucleotide polyadenylation sequence all operatively linked. More particularly, it relates to the method of eliciting an immune response directed against an antigen in a mammal comprising the steps of introducing the expression vector into a cell, expressing the vector to produce an antigen under conditions wherein the antigen is secreted from the cell, endocytosing the secreted antigen into the cell, processing the antigen, and presenting fragments to a receptor to elicit a T-cell response. In addition, this invention relates to a vaccine and a method of use. The invention also relates to the method of identifying MHC-II restricted epitopes.
摘要翻译：本发明涉及表达载体，其中所述表达载体包含多核苷酸启动子序列，编码信号序列的多核苷酸，编码抗原蛋白或肽的多核苷酸，编码细胞结合元件的多核苷酸和全部可操作地连接的多核苷酸聚腺苷酸化序列。更具体地说，本发明涉及在哺乳动物中引发针对抗原的免疫应答的方法，包括以下步骤：将表达载体导入细胞，在其中抗原从细胞分泌的条件下表达载体产生抗原，将分泌的抗原内吞到细胞中，处理抗原，并将片段呈递给受体以引发T细胞应答。此外，本发明涉及疫苗和使用方法。本发明还涉及鉴定MHC-II限制性表位的方法。

9. 发明授权

US08137947B2 Viral vectors whose replication and, optionally, passenger gene are controlled by a gene switch activated by heat in the presence or absence of a small-molecule regulator 有权
标题翻译：在存在或不存在小分子调节剂的情况下，其复制和任选的乘客基因由热激活的基因开关控制的病毒载体
公开(公告)号：US08137947B2
公开(公告)日：2012-03-20
申请号：US12929553
申请日：2011-02-01
申请人： Richard Voellmy
发明人： Richard Voellmy
IPC分类号： C12N7/01 , C12N15/63 , C12N15/86 , A61K48/00
CPC分类号： A61K48/0066 , A61K35/761 , C12N7/00 , C12N15/63 , C12N15/85 , C12N15/86 , C12N2710/10332 , C12N2710/10343 , C12N2800/40 , C12N2820/002 , C12N2820/85 , C12N2830/00 , C12N2830/001 , C12N2830/002 , C12N2830/15 , C12N2830/85 , C12N2840/203
摘要： The present invention relates to conditionally replicating viruses or pairs of viruses containing a gene switch that is activatable by transient heat or other proteotoxic stress in the presence or absence of a small molecule regulator. The gene switch controls the expression of a gene for a protein required for efficient viral replication and may also control the activity of a passenger gene.
摘要翻译：本发明涉及有条件地复制病毒或病毒对，其含有在存在或不存在小分子调节剂时可通过瞬时热或其他蛋白毒性应激激活的基因开关。基因开关控制用于有效病毒复制所需的蛋白质的基因的表达，并且还可以控制乘客基因的活性。

10. 发明申请

US20110281292A1 Compositions and methods for identifying antigens which elicit an immune response 审中-公开
标题翻译：用于鉴定引发免疫应答的抗原的组合物和方法
公开(公告)号：US20110281292A1
公开(公告)日：2011-11-17
申请号：US12829739
申请日：2010-07-02
申请人： Si-Yi Chen , Zhaoyang You
发明人： Si-Yi Chen , Zhaoyang You
IPC分类号： C12Q1/02
CPC分类号： C12N15/1086 , A61K38/00 , A61K39/00 , A61K39/0011 , A61K39/12 , A61K39/292 , A61K47/646 , A61K48/00 , A61K2039/515 , A61K2039/5154 , A61K2039/5156 , A61K2039/53 , A61K2039/55533 , A61K2039/57 , A61K2039/6056 , C07K14/005 , C07K2319/02 , C07K2319/30 , C07K2319/74 , C12N15/86 , C12N2730/10122 , C12N2730/10134 , C12N2740/10043 , C12N2740/10051 , C12N2740/13043 , C12N2740/13051 , C12N2740/15043 , C12N2740/15051 , C12N2820/85 , C12N2840/20 , C12N2840/203 , C12Q1/6883 , C12Q1/6886 , C12Q1/70
摘要： This invention relates to an expression vector wherein said expression vector comprises a polynucleotide promoter sequence, a polynucleotide encoding a signal sequence, a polynucleotide encoding an antigen protein or peptide, a polynucleotide encoding a cell binding element, and a polynucleotide polyadenylation sequence all operatively linked. More particularly, it relates to the method of eliciting an immune response directed against an antigen in a mammal comprising the steps of introducing the expression vector into a cell, expressing the vector to produce an antigen under conditions wherein the antigen is secreted from the cell, endocytosing the secreted antigen into the cell, processing the antigen, and presenting fragments to a receptor to elicit a T-cell response. In addition, this invention relates to a vaccine and a method of use. The invention also relates to the method of identifying MHC-II restricted epitopes.
摘要翻译：本发明涉及表达载体，其中所述表达载体包含多核苷酸启动子序列，编码信号序列的多核苷酸，编码抗原蛋白或肽的多核苷酸，编码细胞结合元件的多核苷酸和全部可操作地连接的多核苷酸聚腺苷酸化序列。更具体地说，本发明涉及在哺乳动物中引发针对抗原的免疫应答的方法，包括以下步骤：将表达载体导入细胞，在其中抗原从细胞分泌的条件下表达载体产生抗原，将分泌的抗原内吞到细胞中，处理抗原，并将片段呈递给受体以引发T细胞应答。此外，本发明涉及疫苗和使用方法。本发明还涉及鉴定MHC-II限制性表位的方法。

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