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1. 发明申请

US20040268443A1 Transgenic expression of a phytochrome a gene 有权
标题翻译：植物色素a基因的转基因表达
公开(公告)号：US20040268443A1
公开(公告)日：2004-12-30
申请号：US10834786
申请日：2004-04-29
发明人： Ray J. Wu , Ajay K. Garg , Ju-Kon Kim
IPC分类号： C12N015/82 , C12N015/87 , A01H005/00 , C12N015/09 , C12N015/63 , C12N005/10 , A01H005/10
CPC分类号： C12N15/8261 , Y02A40/146
摘要： An isolated nucleic acid construct including a nucleic acid molecule encoding a light-labile, phytochrome A, a light-inducible promoter which is 5null to the nucleic acid molecule encoding a light-labile, phytochrome A, and a terminator region which is 3null to the nucleic acid molecule encoding a light-labile, phytochrome A is disclosed. Methods for regulating a plant's canopy architecture and regulating a plant's seed yield, which involve transgenic plants or transgenic plant seeds including an isolated nucleic acid construct according to the present invention, are also disclosed.

2. 发明申请

US20040250309A1 Novel compositions with chitinase activity 有权
标题翻译：具有几丁质酶活性的新型组合物
公开(公告)号：US20040250309A1
公开(公告)日：2004-12-09
申请号：US10389432
申请日：2003-03-14
申请人： Verdia, Inc.
发明人： Mathias L. Muller , Thom True , Carl R. Simmons , Nasser Yalpani
IPC分类号： C12N015/82 , C07H021/04 , C12N015/87 , A01H005/00 , C12N015/09 , C12N015/63 , C07K014/00 , C07K016/00 , C12N015/29
CPC分类号： C12Y302/01014 , C12N9/2442 , C12N15/8282 , C12N15/8286 , Y02A40/162
摘要： The present invention provides compositions with chitinase activity and methods for using such compositions to enhance resistance of plants to fungal infections.
摘要翻译：本发明提供具有几丁质酶活性的组合物和使用这些组合物增强植物对真菌感染的抗性的方法。

3. 发明申请

US20040248303A1 Inducible promoter 失效
标题翻译：诱导启动子
公开(公告)号：US20040248303A1
公开(公告)日：2004-12-09
申请号：US10760752
申请日：2004-01-20
申请人： Syngenta Participations AG
发明人： Rebecca M. Cade , Robert A. Dietrich
IPC分类号： C12N015/82 , C07H021/04 , C12N015/09 , C12N015/63 , C12N005/10
CPC分类号： C12N15/8281 , C07K14/415 , C12N15/8282
摘要： The Arabidopsis NI16 gene was isolated in a yeast 2-hybrid screen via its interaction with the NIM1 protein and encodes a protein involved in the regulation of SAR gene expression in plants. NI16 is strongly induced in NIM1-overexpressing plants treated with benzo(1,2,3)thiadiazole-7-carbothioic acid S-methyl ester (BTH). The nucleic acid sequence of the Arabidopsis NI16 promoter is disclosed herein.
摘要翻译：通过与NIM1蛋白的相互作用，在酵母2-杂交筛选中分离拟南芥NI16基因，并编码参与植物中SAR基因表达调控的蛋白质。 NI16在用苯并（1,2,3）噻二唑-7-硫代硫酸S-甲酯（BTH）处理的NIM1过表达植物中强烈诱导。本文公开了拟南芥NI16启动子的核酸序列。

4. 发明申请

US20040216185A1 Engineering beta-ketoacyl ACP synthase for novel substrate specificity 审中-公开
标题翻译：工程β-酮酰胆碱ACP合酶用于新颖的底物特异性
公开(公告)号：US20040216185A1
公开(公告)日：2004-10-28
申请号：US10851894
申请日：2004-05-24
发明人： Katayoon Dehesh , Dale Val
IPC分类号： C12N015/82 , C12N015/87 , C12N005/04 , C12N015/63 , A01H005/00
CPC分类号： C12N9/1029 , C12N15/8247 , C12P7/6409 , C12P7/6463
摘要： Methods of altering substrate specificity of beta-ketoacyl-ACP synthase, and engineered beta-ketoacyl-ACP synthases so produced are provided. DNA sequences and constructs for expression of engineered beta-ketoacyl-ACP synthases, as well as the novel beta-ketoacyl-ACP synthases produced therefrom are also provided. Such DNA sequences may be used for expression of the engineered beta-ketoacyl-ACP synthases in host cells, particularly seed cells of oilseed crop plants, for the modification of fatty acid composition.
摘要翻译：提供了改变β-酮酰基-ACP合成酶底物特异性的方法和如此制备的工程化β-酮酰基-ACP合成酶。还提供了用于表达工程化β-酮酸酰-ACP合酶的DNA序列和构建体，以及由其产生的新型β-酮酰基-ACP合成酶。这样的DNA序列可用于在宿主细胞，特别是油菜作物植物的种子细胞中表达改造的β-酮酸酰-ACP合酶，用于改变脂肪酸组成。

5. 发明申请

US20040214318A1 Method for enhancing RNA or protein production using non-native 5' untranslated sequences in recombinant viral nucleic acids 审中-公开
标题翻译：在重组病毒核酸中使用非天然5'非翻译序列增强RNA或蛋白质产生的方法
公开(公告)号：US20040214318A1
公开(公告)日：2004-10-28
申请号：US10858775
申请日：2004-06-01
发明人： Sean Chapman , William O. Dawson , Jonothan Donson , Monto H. Kumagai , Dennis J. Lewandowski , John A. Lindbo , Gregory P. Pogue , Shailaja Shivprasad
IPC分类号： C07H021/04 , C12N015/09 , C12N015/63 , C12N005/04 , C12N015/87 , C12N015/00 , C12N015/70 , C12N015/85
CPC分类号： C12N9/2422 , A01H1/04 , A01H5/12 , C07K14/61 , C07K14/805 , C07K14/82 , C07K16/3061 , C07K2317/13 , C07K2317/622 , C12N15/1034 , C12N15/8203 , C12N15/8216 , C12N15/8245 , C12N15/8257 , C12N15/8258 , C12Q1/68
摘要： The present invention provides a method for enhancing the production of RNAs or proteins in a plant host using either non-native 5null untranslated sequences or artificial leader sequences. Preferably, commercially useful proteins, polypeptides, or fusion products thereof are produced, such as, enzymes, antibodies, hormones, pharmaceuticals, vaccines, pigments, anti-microbial polypeptides, and the like. The non-native 5null untranslated enhancers may also be effective in many different types of transcription or translation systems, such as bacterial and animal systems.
摘要翻译：本发明提供了使用非天然5'非翻译序列或人造前导序列来增强植物宿主中RNA或蛋白质产生的方法。优选地，产生商业上有用的蛋白质，多肽或其融合产物，例如酶，抗体，激素，药物，疫苗，颜料，抗微生物多肽等。非天然的5'非翻译增强子也可以在许多不同类型的转录或翻译系统中有效，例如细菌和动物系统。

6. 发明申请

US20040210957A1 Cloning and characterization of the broad-spectrum resistance gene P12 失效
标题翻译：广谱抗性基因P12的克隆和表征
公开(公告)号：US20040210957A1
公开(公告)日：2004-10-21
申请号：US10656394
申请日：2003-09-05
申请人： The Ohio State University
发明人： Shaohong Qu , Guo-liang Wang , Bo Zhou
IPC分类号： C12N015/29 , C07H021/04 , C12N015/82 , A01H005/00 , C12N015/87 , C12N015/63
CPC分类号： C12N15/8282 , C07K14/415
摘要： Compositions and methods for enhancing or creating plant disease resistance to plant pests are provided. Transforming a plant with a novel rice Pi2-like disease resistance gene of the invention enhances disease resistance of the plant. Transformed plants, plant cells, tissues, and seed having enhanced disease resistance are also provided.
摘要翻译：提供了用于增强或产生对植物害虫的植物病害抗性的组合物和方法。用本发明的新型水稻Pi2样抗病基因转化植物提高了植物的抗病性。还提供了具有增强的抗病性的转化植物，植物细胞，组织和种子。

7. 发明申请

US20040210956A1 Expression of spider silk proteins in higher plants 有权
标题翻译：蜘蛛丝蛋白在高等植物中的表达
公开(公告)号：US20040210956A1
公开(公告)日：2004-10-21
申请号：US10479638
申请日：2004-05-05
发明人： Don A. Roth , Randolph V. Lewis
IPC分类号： C12N015/82 , C12N015/87 , C12N015/63 , C07K014/00 , C12N005/04
CPC分类号： C07K14/43518 , C12N15/8257
摘要： The present invention relates to the fields of molecular biology and plant biology. Specifically, the invention is directed to the methods for expressing spider silk proteins in plants and the synthesis and purification of spider silk proteins therefrom.
摘要翻译：本发明涉及分子生物学和植物生物学领域。具体地说，本发明涉及在植物中表达蜘蛛丝蛋白的方法以及从其中合成和提纯蜘蛛丝蛋白。

8. 发明申请

US20040208847A1 Method and vectors for selectively transducing retinal pigment epithelium cells 审中-公开
标题翻译：用于选择性转导视网膜色素上皮细胞的方法和载体
公开(公告)号：US20040208847A1
公开(公告)日：2004-10-21
申请号：US10400531
申请日：2003-03-28
发明人： Fabienne Rolling , Michel Weber
IPC分类号： A01N063/00 , A01N065/00 , A61K048/00 , C12N015/00 , C12N015/09 , C12N015/63 , C12N015/70 , C12N015/74
CPC分类号： C12N15/86 , A61K48/0075 , C12N2750/14143
摘要： A method for selectively transducing retinal pigment epithelium (RPE) cells in an eye of a mammal, comprises administering to the mammal a vector particle exhibiting an AAV-4 capsid protein.
摘要翻译：用于在哺乳动物的眼睛中选择性转导视网膜色素上皮（RPE）细胞的方法包括向哺乳动物施用呈现AAV-4衣壳蛋白的载体颗粒。

9. 发明申请

US20040205841A1 Pathogen induced promoters 审中-公开
标题翻译：病原体诱导的启动子
公开(公告)号：US20040205841A1
公开(公告)日：2004-10-14
申请号：US10638464
申请日：2003-08-12
发明人： Christopher Lawrence
IPC分类号： C12N015/82 , C07H021/04 , A01H001/00 , C12N015/87 , C12N015/63
CPC分类号： C12N15/8282 , C12N15/8239
摘要： Pathogen-inducible plant promoters are identified. The promoters control expression of nullhypersensitive responsenull proteins, including a protease inhibitor, an isocitrate lyase, and a glycoprotein. Heterologous gene sequences are produced by operably linking a promoter according to the present invention with a gene to be expressed in a transformed plant. Transformed plants are made by transforming a vector with a heterologous gene according to the present invention and then transforming the plant with the transformed vector. The transformed plants are capable of expressing a pre-selected protein in response to challenge by a plant pathogen, for example tobacco blue moldnullP. tabacina.
摘要翻译：确定病原体诱导型植物启动子。启动子控制“过敏反应”蛋白质的表达，包括蛋白酶抑制剂，异柠檬酸裂解酶和糖蛋白。通过将根据本发明的启动子与在转化植物中表达的基因可操作地连接来产生异源基因序列。通过用根据本发明的异源基因转化载体然后用转化的载体转化植物来制备转化的植物。转化的植物能够响应于植物病原体（例如烟草蓝霉素-P）的攻击而表达预先选择的蛋白质。 tabacina

10. 发明申请

US20040194155A1 HBV mutations associated with reduced susceptibility to adefovir 审中-公开
标题翻译：与阿德福韦敏感性降低相关的HBV突变
公开(公告)号：US20040194155A1
公开(公告)日：2004-09-30
申请号：US10678443
申请日：2003-10-01
申请人： GILEAD SCIENCES, INC.
发明人： William E. Delaney , Xiaofeng Xiong
IPC分类号： A01K067/00 , A01K067/033 , C12N015/00 , C12N015/09 , C12N015/63 , C12N015/70 , C12N015/74 , C12N005/00 , C12N005/02
CPC分类号： C07K14/005 , A61K38/00 , A61K39/00 , A61K2039/505 , C12N9/1276 , C12N2730/10122
摘要： Applicants have identified 5 mutants associated with hepatitis B virus resistance to adefovir, a nucleotide analogue antiviral drug widely employed in the therapy of hepatitis B. In accord with this invention, reverse transcriptase mutants rtN236T, rtA181V, rtA181T and their corresponding surface antigen mutants sL173F and sL172trunc are provided. The mutant proteins, antibodies thereto and nucleic acids encoding the mutants have diagnostic value in monitoring and adjusting patient therapy with adefovir and in the therapy of patients infected with the mutants.
摘要翻译：申请人已经确定了与乙型肝炎病毒抗性相关的5种突变体，阿德福韦，广泛用于乙型肝炎治疗的核苷酸类似物抗病毒药物。根据本发明，逆转录酶突变体rtN236T，rtA181V，rtA181T及其相应的表面抗原突变体sL173F和提供sL172trunc。突变蛋白，其抗体和编码突变体的核酸在监测和调整阿德福韦的患者治疗以及感染突变体的患者的治疗方面具有诊断价值。

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