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    • 5. 发明授权
    • Continuously variable transmission
    • 连续可变传动
    • US07309067B2
    • 2007-12-18
    • US11108663
    • 2005-04-19
    • Akihiko Kita
    • Akihiko Kita
    • F16H37/02
    • F16H37/022F16H61/12
    • An input shaft, a stepless speed change unit, a forward-reverse switching mechanism, and an output shaft are arranged in that order in a power transmitting path of a continuously variable transmission. The forward-reverse switching mechanism includes i) a gear mechanism having a sun gear which rotates in the same direction as the rotation output from the stepless speed change unit, a carrier which is always fixed (stationary), and a ring gear which rotates in the reverse direction, ii) a clutch between the sun gear and the output shaft, and iii) a clutch between the ring gear and the output shaft. This structure enables the transmission of power between the gear mechanism and the stepless speed change unit and the vehicle drive wheels to be interrupted by the clutches when the vehicle is being towed or is stopped suddenly, and also obviates the need for a brake able to withstand a large amount of torque.
    • 在无级变速器的动力传递路径中依次配置输入轴,无级变速单元,正向反向切换机构和输出轴。 前进后退切换机构包括:i)齿轮机构,其具有与从无级变速单元的旋转输出方向相同的方向旋转的太阳齿轮,总是固定(静止)的载体,以及环形齿轮, 相反方向,ii)太阳齿轮与输出轴之间的离合器,以及iii)齿圈与输出轴之间的离合器。 这种结构使得当车辆被牵引或突然停止时,齿轮机构和无级变速单元和车辆驱动轮之间的动力传递被离合器中断,并且也避免了对能够承受的制动器的需要 大量的扭矩。
    • 6. 发明授权
    • Method for effecting site-directed mutagenesis
    • 进行定点突变的方法
    • US06448048B1
    • 2002-09-10
    • US09214146
    • 1998-12-29
    • Jun TomonoAkihiko KitaSusumu TsunasawaIkunoshin Kato
    • Jun TomonoAkihiko KitaSusumu TsunasawaIkunoshin Kato
    • C12N1564
    • C12N15/102
    • A method for performing site-directed mutagenesis characterized in that the method includes the step of carrying out PCR by the use of a double-stranded DNA vector having one or more amber codons, the vector resulting from insertion of a target DNA fragment for site-directed mutagenesis, and at least two kinds of selection primers; and a kit for site-directed mutagenesis for use in the above method, characterized in that the kit includes amber codon reversion primers. According to the present invention, there can be provided a method for performing site-directed mutagenesis and a kit, which is useful for genetic engineering or protein engineering, more simply and rapidly. By using the method and the kit of the present invention, it is possible to efficiently obtain a mutation-introduced gene at the desired position by simply transforming a host with a PCR product obtained by PCR.
    • 一种用于进行定点诱变的方法,其特征在于该方法包括通过使用具有一个或多个琥珀密码子的双链DNA载体进行PCR的步骤,所述双链DNA载体由插入靶DNA片段所产生的载体, 定向诱变和至少两种选择引物; 以及用于上述方法的用于定点诱变的试剂盒,其特征在于所述试剂盒包括琥珀密码子反转引物。 根据本发明,可以提供用于进行定点诱变的方法和用于遗传工程或蛋白质工程的试剂盒,其更简单和快速。 通过使用本发明的方法和试剂盒,通过用PCR获得的PCR产物简单地转化宿主,可以有效地获得所需位置的突变导入基因。
    • 8. 发明申请
    • Continuously variable transmission
    • 连续可变传动
    • US20060030447A1
    • 2006-02-09
    • US11108663
    • 2005-04-19
    • Akihiko Kita
    • Akihiko Kita
    • F16H47/08
    • F16H37/022F16H61/12
    • An input shaft, a stepless speed change unit, a forward-reverse switching mechanism, and an output shaft are arranged in that order in a power transmitting path of a continuously variable transmission. The forward-reverse switching mechanism includes i) a gear mechanism having a sun gear which rotates in the same direction as the rotation output from the stepless speed change unit, a carrier which is always fixed (stationary), and a ring gear which rotates in the reverse direction, ii) a clutch between the sun gear and the output shaft, and iii) a clutch between the ring gear and the output shaft. This structure enables the transmission of power between the gear mechanism and the stepless speed change unit and the vehicle drive wheels to be interrupted by the clutches when the vehicle is being towed or is stopped suddenly, and also obviates the need for a brake able to withstand a large amount of torque.
    • 在无级变速器的动力传递路径中依次配置输入轴,无级变速单元,正向反向切换机构和输出轴。 前进后退切换机构包括:i)齿轮机构,其具有与从无级变速单元的旋转输出方向相同的方向旋转的太阳齿轮,总是固定(静止)的载体,以及环形齿轮, 相反方向,ii)太阳齿轮与输出轴之间的离合器,以及iii)齿圈与输出轴之间的离合器。 这种结构使得当车辆被牵引或突然停止时,齿轮机构和无级变速单元和车辆驱动轮之间的动力传递被离合器中断,并且也避免了对能够承受的制动器的需要 大量的扭矩。
    • 10. 发明申请
    • HON-SHIMEJI MUSHROOM-FUNGAL BED CULTURE
    • HON-SHIMEJI MUSHROOM - FUNGAL BED CULTURE
    • US20090148926A1
    • 2009-06-11
    • US12272758
    • 2008-11-17
    • Takashi KAWAIKatsuhiko KusakabeAkihiko KitaIkunoshin Kato
    • Takashi KAWAIKatsuhiko KusakabeAkihiko KitaIkunoshin Kato
    • C12N1/14
    • C12N1/14A01G18/00A01G18/10
    • Problem: To provide a fungal bed culture enabling stable production of a hon-shimeji mushroom in large scale commercial cultivation as well as a method of fungal bed cultivation of a hon-shimeji mushroom using the fungal bed culture.Solution: There is provided a fungal bed culture of a hon-shimeji mushroom inoculated with a liquid seed culture characterized in that the surface of a culture medium for cultivation has a liquid seed culture inoculated portion and a liquid seed culture non-inoculated portion as well as a fungal bed cultivation method of a hon-shimeji mushroom characterized by generating a fruit body from the fungal bed culture. According to the present invention improves the formation rate of budlet in the fungal bed cultivation of a hon-shimeji mushroom, thereby enabling stable production of a hon-shimeji mushroom in large scale commercial cultivation.
    • 问题:提供一种真菌床文化,能够在大规模商业栽培中稳定生产一种香菇菇,以及使用真菌床培养方法真菌床栽培枸杞子蘑菇。 解决方案:提供了接种液体种子培养物的枸杞蘑菇的真菌床培养物,其特征在于用于培养的培养基的表面具有液体种子培养接种部分和液体种子培养未接种部分 作为真菌床栽培方法,以真菌床培养物产生果实为特征。 根据本发明,提高了枸杞蘑菇真菌床栽培中芽的形成速率,从而在大规模商业栽培中稳定地生产了一种枸杞子蘑菇。