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1. 发明授权

US08278508B2 Hon-shimeji mushroom-fungal bed culture 失效
标题翻译：香菇子蘑菇真菌床文化
公开(公告)号：US08278508B2
公开(公告)日：2012-10-02
申请号：US12272758
申请日：2008-11-17
申请人： Takashi Kawai , Katsuhiko Kusakabe , Akihiko Kita , Ikunoshin Kato
发明人： Takashi Kawai , Katsuhiko Kusakabe , Akihiko Kita , Ikunoshin Kato
IPC分类号： A01H15/00 , A01N63/00 , A01N63/04 , C12N1/14 , C12N1/16
CPC分类号： C12N1/14 , A01G18/00 , A01G18/10
摘要： The present invention provides a fungal bed culture of a hon-shimeji mushroom inoculated with a liquid seed culture wherein the surface of a culture medium for cultivation has a liquid seed culture inoculated portion and a liquid seed culture non-inoculated portion as well as provides a fungal bed cultivation method of a hon-shimeji mushroom which generates a fruit body from the fungal bed culture. According to the present invention, the formation rate of budlet in the fungal bed cultivation of a hon-shimeji mushroom is improved, thereby enabling stable production of a hon-shimeji mushroom in large scale commercial cultivation.
摘要翻译：本发明提供了接种液体种子培养物的枸杞蘑菇的真菌培养物，其中用于培养的培养基的表面具有液体种子培养接种部分和液体种子培养未接种部分，并且提供真菌床栽培方法，从真菌培养物中产生果实。根据本发明，提高了一种枸杞菇真菌床栽培中芽的形成速率，从而能够在大规模商业栽培中稳定地生产枸杞子蘑菇。

2. 发明申请

US20090148926A1 HON-SHIMEJI MUSHROOM-FUNGAL BED CULTURE 失效
标题翻译： HON-SHIMEJI MUSHROOM - FUNGAL BED CULTURE
公开(公告)号：US20090148926A1
公开(公告)日：2009-06-11
申请号：US12272758
申请日：2008-11-17
申请人： Takashi KAWAI , Katsuhiko Kusakabe , Akihiko Kita , Ikunoshin Kato
发明人： Takashi KAWAI , Katsuhiko Kusakabe , Akihiko Kita , Ikunoshin Kato
IPC分类号： C12N1/14
CPC分类号： C12N1/14 , A01G18/00 , A01G18/10
摘要： Problem: To provide a fungal bed culture enabling stable production of a hon-shimeji mushroom in large scale commercial cultivation as well as a method of fungal bed cultivation of a hon-shimeji mushroom using the fungal bed culture.Solution: There is provided a fungal bed culture of a hon-shimeji mushroom inoculated with a liquid seed culture characterized in that the surface of a culture medium for cultivation has a liquid seed culture inoculated portion and a liquid seed culture non-inoculated portion as well as a fungal bed cultivation method of a hon-shimeji mushroom characterized by generating a fruit body from the fungal bed culture. According to the present invention improves the formation rate of budlet in the fungal bed cultivation of a hon-shimeji mushroom, thereby enabling stable production of a hon-shimeji mushroom in large scale commercial cultivation.
摘要翻译：问题：提供一种真菌床文化，能够在大规模商业栽培中稳定生产一种香菇菇，以及使用真菌床培养方法真菌床栽培枸杞子蘑菇。解决方案：提供了接种液体种子培养物的枸杞蘑菇的真菌床培养物，其特征在于用于培养的培养基的表面具有液体种子培养接种部分和液体种子培养未接种部分作为真菌床栽培方法，以真菌床培养物产生果实为特征。根据本发明，提高了枸杞蘑菇真菌床栽培中芽的形成速率，从而在大规模商业栽培中稳定地生产了一种枸杞子蘑菇。

3. 发明授权

US07984584B2 Method for fungal bed cultivation of mushroom 失效
标题翻译：蘑菇真菌床栽培方法
公开(公告)号：US07984584B2
公开(公告)日：2011-07-26
申请号：US12129557
申请日：2008-05-29
申请人： Takashi Kawai , Hiroko Ohta , Katsuhiko Kusakabe , Akihiko Kita , Ikunoshin Kato
发明人： Takashi Kawai , Hiroko Ohta , Katsuhiko Kusakabe , Akihiko Kita , Ikunoshin Kato
IPC分类号： A01G1/04
CPC分类号： A01G18/00
摘要： Problem: To provide a method for fungal bed cultivation of a mushroom of a large size having an excellent shape and crunchy texture, a mushroom cutting useful for the fungal bed cultivation method, a culture medium for fungal bed cultivation into which the cutting is transplanted, and a culture medium suitable for fungal bed cultivation.Solution: A method for cultivating a mushroom in a fungal bed comprising a step of transplanting an isolated cutting of the mushroom into a culture medium for fungal bed cultivation, an isolated cutting of a mushroom to be used in the method, and a culture medium for fungal bed cultivation of a mushroom into which the cutting is transplanted.
摘要翻译：问题：提供一种具有优良形状和松脆质地的大尺寸蘑菇的真菌床栽培方法，用于真菌床栽培方法的蘑菇切片，移植切割的真菌床栽培培养基，和适合真菌床栽培的培养基。解决方案：一种在真菌床中培养蘑菇的方法，包括将分离的蘑菇切片移植到用于真菌床培养的培养基中的步骤，用于该方法的蘑菇的分离切割，以及用于真菌床栽培的蘑菇，切割被移植到其中。

4. 发明授权

US07971388B2 Method for cultivating hon-shimeji mushroom on fungal bed 失效
标题翻译：在真菌床上培养香菇菇的方法
公开(公告)号：US07971388B2
公开(公告)日：2011-07-05
申请号：US12178830
申请日：2008-07-24
申请人： Takashi Kawai , Katsuhiko Kusakabe , Akihiko Kita , Ikunoshin Kato
发明人： Takashi Kawai , Katsuhiko Kusakabe , Akihiko Kita , Ikunoshin Kato
IPC分类号： A01G1/04
CPC分类号： A01G18/10 , A01G18/20
摘要： According to the present invention, there is provided a fungal bed cultivation method of a hon-shimeji mushroom, wherein induction of primordia of a fruit body is conducted by illumination under incubation conditions, and an ongoing culture for fungal bed cultivation of a hon-shimeji mushroom, wherein primordia of a fruit body have been formed thereon during incubation. The culture medium is maintained in a clean state and it is advantageous because the formation of primordia of a fruit body can be carried out in a clean environment and the culture medium wherein primordia of a fruit body have been formed can be transferred to distant mushroom cultivation facilities under clean conditions as such.
摘要翻译：根据本发明，提供了一种枸杞菇的真菌床栽培方法，其中通过在孵育条件下照明进行果实诱导，并且正在进行的培养真菌床栽培蘑菇，其中在孵育期间在其上形成果体的原基。保持培养基处于清洁状态，因为可以在清洁的环境中进行果实的原基形成，并且可以将其中形成果实原基的培养基转移到远距离的蘑菇栽培清洁条件下的设施。

5. 发明申请

US20100139157A1 FUNGAL BED CULTIVATION METHOD OF HON-SHIMEJI MUSHROOM 审中-公开
标题翻译： HON-SHIMEJI MUSHROOM的真菌床培养方法
公开(公告)号：US20100139157A1
公开(公告)日：2010-06-10
申请号：US12627341
申请日：2009-11-30
申请人： Takashi Kawai , Mayu Hashimoto , Takeshi Sugimori , Katsuhiko Kusakabe , Akihiko Kita , Ikunoshin Kato
发明人： Takashi Kawai , Mayu Hashimoto , Takeshi Sugimori , Katsuhiko Kusakabe , Akihiko Kita , Ikunoshin Kato
IPC分类号： A01G1/04
CPC分类号： A01G18/00
摘要： A fungal bed cultivation method of a hon-shimeji mushroom, in which the sprouting step and/or fruit body growing step is carried out under an environmental condition of high CO2 concentration, is provided. Examples of the environmental condition of high CO2 concentration include a CO2 concentration of 2,500 ppm or more in the sprouting step and a CO2 concentration of 5,000 ppm or more in the fruit body growing step. Since the formation ratio of a budlet in the fungal bed cultivation of a hon-shimeji mushroom is improved by embodiments of the present invention, stable production of a hon-shimeji mushroom by its large scale commercial cultivation becomes possible.
摘要翻译：提供了一种在CO 2浓度高的环境条件下进行发芽步骤和/或果实生长步骤的枸杞蘑菇的真菌培育方法。 CO 2浓度高的环境条件的实例包括发芽步骤中的二氧化碳浓度为2500ppm以上，果实生长步骤中的CO 2浓度为5000ppm以上。由于通过本发明的实施例改善了香菇菌种的真菌床栽培中的芽的形成比例，因此可以通过大规模的商业栽培稳定地生产枸杞子蘑菇。

6. 发明申请

US20090025286A1 METHOD FOR CULTIVATING HON-SHIMEJI MUSHROOM ON FUNGAL BED 失效
标题翻译：在真菌床上培养人造丝霉菌的方法
公开(公告)号：US20090025286A1
公开(公告)日：2009-01-29
申请号：US12178830
申请日：2008-07-24
申请人： Takashi Kawai , Katsuhiko Kusakabe , Akihiko Kita , Ikunoshin Kato
发明人： Takashi Kawai , Katsuhiko Kusakabe , Akihiko Kita , Ikunoshin Kato
IPC分类号： A01G1/04
CPC分类号： A01G18/10 , A01G18/20
摘要： According to the present invention, there is provided a fungal bed cultivation method of a hon-shimeji mushroom, wherein induction of primordia of a fruit body is conducted by illumination under incubation conditions, and an ongoing culture for fungal bed cultivation of a hon-shimeji mushroom, wherein primordia of a fruit body have been formed thereon during incubation. The culture medium is maintained in a clean state and it is advantageous because the formation of primordia of a fruit body can be carried out in a clean environment and the culture medium wherein primordia of a fruit body have been formed can be transferred to distant mushroom cultivation facilities under clean conditions as such.
摘要翻译：根据本发明，提供了一种枸杞菇的真菌床栽培方法，其中通过在孵育条件下照明进行果实诱导，并且正在进行的培养真菌床栽培蘑菇，其中在孵育期间在其上形成果体的原基。保持培养基处于清洁状态，因为可以在清洁的环境中进行果实的原基形成，并且可以将其中形成果实原基的培养基转移到远距离的蘑菇栽培清洁条件下的设施。

7. 发明授权

US06448048B1 Method for effecting site-directed mutagenesis 失效
标题翻译：进行定点突变的方法
公开(公告)号：US06448048B1
公开(公告)日：2002-09-10
申请号：US09214146
申请日：1998-12-29
申请人： Jun Tomono , Akihiko Kita , Susumu Tsunasawa , Ikunoshin Kato
发明人： Jun Tomono , Akihiko Kita , Susumu Tsunasawa , Ikunoshin Kato
IPC分类号： C12N1564
CPC分类号： C12N15/102
摘要： A method for performing site-directed mutagenesis characterized in that the method includes the step of carrying out PCR by the use of a double-stranded DNA vector having one or more amber codons, the vector resulting from insertion of a target DNA fragment for site-directed mutagenesis, and at least two kinds of selection primers; and a kit for site-directed mutagenesis for use in the above method, characterized in that the kit includes amber codon reversion primers. According to the present invention, there can be provided a method for performing site-directed mutagenesis and a kit, which is useful for genetic engineering or protein engineering, more simply and rapidly. By using the method and the kit of the present invention, it is possible to efficiently obtain a mutation-introduced gene at the desired position by simply transforming a host with a PCR product obtained by PCR.
摘要翻译：一种用于进行定点诱变的方法，其特征在于该方法包括通过使用具有一个或多个琥珀密码子的双链DNA载体进行PCR的步骤，所述双链DNA载体由插入靶DNA片段所产生的载体，定向诱变和至少两种选择引物; 以及用于上述方法的用于定点诱变的试剂盒，其特征在于所述试剂盒包括琥珀密码子反转引物。根据本发明，可以提供用于进行定点诱变的方法和用于遗传工程或蛋白质工程的试剂盒，其更简单和快速。通过使用本发明的方法和试剂盒，通过用PCR获得的PCR产物简单地转化宿主，可以有效地获得所需位置的突变导入基因。

8. 发明授权

US06927289B2 Sulfated fucan oligosaccharide 失效
标题翻译：硫酸化的fucan寡糖
公开(公告)号：US06927289B2
公开(公告)日：2005-08-09
申请号：US10694352
申请日：2003-10-28
申请人： Takeshi Sakai , Hitomi Amarume , Takashi Kawai , Kaoru Kojima , Kazuo Shimanaka , Katsushige Ikai , Ikunoshin Kato
发明人： Takeshi Sakai , Hitomi Amarume , Takashi Kawai , Kaoru Kojima , Kazuo Shimanaka , Katsushige Ikai , Ikunoshin Kato
IPC分类号： C08B37/00 , C07H5/00
CPC分类号： C08B37/006
摘要： A smaller molecule obtainable by allowing a sulfated fucan-digesting enzyme which digests a novel sulfated polysaccharide derived from an alga belonging to Laminariales to act on a sulfated fucan, and a method for producing the same.
摘要翻译：通过使来自属于层状藻酸盐的藻类的新型硫酸化多糖消化的硫酸化的岩藻糖消化酶作用于硫酸化的岩藻石上的小分子及其制造方法。

9. 发明授权

US06720419B2 Sulfated fucan oligosaccharide 失效
标题翻译：硫酸化的fucan寡糖
公开(公告)号：US06720419B2
公开(公告)日：2004-04-13
申请号：US10278942
申请日：2002-10-24
申请人： Takeshi Sakai , Hitomi Amarume , Takashi Kawai , Kaoru Kojima , Kazuo Shimanaka , Katsushige Ikai , Ikunoshin Kato
发明人： Takeshi Sakai , Hitomi Amarume , Takashi Kawai , Kaoru Kojima , Kazuo Shimanaka , Katsushige Ikai , Ikunoshin Kato
IPC分类号： C08B3700
CPC分类号： C08B37/006
摘要： A smaller molecule obtainable by allowing a sulfated fucan-digesting enzyme which digests a novel sulfated polysaccharide derived from an alga belonging to Laminariales to act on a sulfated fucan, and a method for producing the same.
摘要翻译：通过使来自属于层状藻酸盐的藻类的新型硫酸化多糖消化的硫酸化的岩藻糖消化酶作用于硫酸化的岩藻石上的小分子及其制造方法。

10. 发明申请

US20080038289A1 Antitumor Agent 审中-公开
标题翻译：抗肿瘤剂
公开(公告)号：US20080038289A1
公开(公告)日：2008-02-14
申请号：US11596395
申请日：2005-05-30
申请人： Takashi Kawai , Shigetoshi Mizutani , Tatsuji Enoki , Hiroaki Sagawa , Takeshi Sakai , Kazuo Shimanaka , Ikunoshin Kato
发明人： Takashi Kawai , Shigetoshi Mizutani , Tatsuji Enoki , Hiroaki Sagawa , Takeshi Sakai , Kazuo Shimanaka , Ikunoshin Kato
IPC分类号： A61K36/06 , A61K31/045 , A61P35/00
CPC分类号： A61K36/07 , A23L33/105 , A61K31/047
摘要： According to the present invention, an antitumor agent, an apoptosis-inducing agent or an angiogenesis-suppressing agent having the effect even via an oral route, containing a compound contained in an inexpensive edible basidiomycete or a composition derived from an edible basidiomycete, and a food having the antitumor activity, the apoptosis-inducing activity or the angiogenesis-suppressing activity containing the compound or the composition can be obtained. Therefore, the present invention is particularly useful, especially in the field of a medicine and a health food.
摘要翻译：根据本发明，通过口服途径具有效果的抗肿瘤剂，细胞凋亡诱导剂或血管生成抑制剂，其含有廉价的可食用担子菌或含有可食用担子菌的组合物，以及可以获得具有抗肿瘤活性，含有化合物或组合物的细胞凋亡诱导活性或血管生成抑制活性的食品。因此，本发明特别有用，特别是在药物和保健食品领域。

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