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    • 1. 发明申请
    • METHOD AND KIT FOR DETECTION/IDENTIFICATION OF VIRUS-INFECTED CELL
    • 用于检测/鉴定病毒感染细胞的方法和工具包
    • US20110065094A1
    • 2011-03-17
    • US12933736
    • 2009-03-17
    • Hiroshi KimuraYukihiro Nishiyama
    • Hiroshi KimuraYukihiro Nishiyama
    • C12Q1/70
    • C12Q1/701C12Q1/6804G01N33/56994G01N2333/05C12Q2565/626
    • Disclosed is a means for specifically detecting and identifying a virus-infected cell in a floating cell system by a simple manipulation and with high sensitivity. A virus-infected cell can be detected and identified by the following steps (1) to (5): (1) adding a first labeled antibody which has been labeled with a first labeling substance and is directed against a cell surface antigen specific to a target cell to a sample and allowing the mixture to react; (2) immobilizing a protein in the presence of an RNAstabilizing agent; (3) treating the protein with a surfactant; (4) adding a labeled nucleic acid probe for a nucleic acid specific to a target virus to cause the hybridization; and (5) detecting a cell labeled with both the first labeled antibody and the labeled nucleic acid probe by flow cytometry.
    • 公开了通过简单的操作和高灵敏度来特异性检测和识别浮游细胞系统中的病毒感染细胞的方法。 可以通过以下步骤(1)至(5)检测和鉴定病毒感染的细胞:(1)加入已经用第一标记物质标记的第一标记抗体,并针对特异性的细胞表面抗原 靶细胞至样品并使混合物反应; (2)在RNA稳定剂存在下固定蛋白质; (3)用表面活性剂处理蛋白质; (4)为靶病毒特异性的核酸加入标记的核酸探针以引起杂交; 和(5)通过流式细胞术检测用第一标记抗体和标记的核酸探针两者标记的细胞。