专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US07807360B2 Method and apparatus for concentrating and amplifying nucleic acid in single micro chamber 有权
标题翻译：在单微室中浓缩和扩增核酸的方法和装置
公开(公告)号：US07807360B2
公开(公告)日：2010-10-05
申请号：US11620961
申请日：2007-01-08
申请人： Young-rok Kim , Jun-hong Min , In-ho Lee , Young-sun Lee , Chang-eun Yoo , Ki-woong Han
发明人： Young-rok Kim , Jun-hong Min , In-ho Lee , Young-sun Lee , Chang-eun Yoo , Ki-woong Han
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , B01L3/5027 , B01L3/5088 , C12Q1/686 , C12Q2565/629 , C12Q2537/149 , C12Q2527/125
摘要： A method of sequentially performing concentration and amplification of nucleic acid in a single micro chamber includes: introducing a nucleic acid-containing sample and a solution including a kosmotropic salt to a micro chamber having a hydrophilic interior surface to concentrate the nucleic acid by binding the nucleic acid on the interior surface of the micro chamber; and performing a polymerase chain reaction (PCR) by adding a PCR mixture to the chamber. Since the nucleic acid is reversibly bound to the interior surface of the micro chamber, PCR yield is higher compared with a surface of aluminum oxide in which irreversible binding occurs. In addition, all processes are sequentially performed in a single micro chamber so that the number of samples, consumables, time, and labor for treatment and analysis can be reduced, detection sensitivity can be improved, and risk of sample cross contamination significantly reduced without sample loss by eliminating transporting of the sample. A complete automated system for concentration and amplification of nucleic acid is thus readily provided.
摘要翻译：在单个微室中依次进行核酸的浓缩和扩增的方法包括：将含核酸的样品和包含可染色盐的溶液引入具有亲水性内表面的微室中，以通过结合核酸来浓缩核酸酸在微室的内表面上; 并通过向该室加入PCR混合物进行聚合酶链式反应（PCR）。由于核酸可逆地结合到微室的内表面，与其中发生不可逆结合的氧化铝的表面相比，PCR产率更高。此外，所有过程都在单个微室中顺序进行，以便可以减少样品数量，消耗品，时间和处理和分析的劳动力，可以提高检测灵敏度，并且样品交叉污染的风险在没有样品时显着降低通过消除样品的运输而损失。因此容易提供用于浓缩和扩增核酸的完整的自动化系统。

2. 发明申请

US20070238114A1 Method and apparatus for disrupting cells and purifying nucleic acid using a single chip 有权
标题翻译：使用单个芯片破坏细胞并纯化核酸的方法和装置
公开(公告)号：US20070238114A1
公开(公告)日：2007-10-11
申请号：US11639794
申请日：2006-12-15
申请人： In-ho Lee , Jun-hong Min , Young-rok Kim , Jae-ho You , Chang-eun Yoo , Jeong-gun Lee , Ki-woong Han
发明人： In-ho Lee , Jun-hong Min , Young-rok Kim , Jae-ho You , Chang-eun Yoo , Jeong-gun Lee , Ki-woong Han
IPC分类号： C12Q1/68 , C12N1/08
CPC分类号： C12Q1/68 , C12M47/06 , C12N15/1006
摘要： Provided herein is a method and apparatus for disrupting cells and purifying nucleic acids in a single chip. The method comprises irradiating a chip with a laser beam, wherein the chip comprises a solid support on which a cell lysis enhancing metal oxide layer, and a cell binding metal oxide layer have been deposited.
摘要翻译：本文提供了在单个芯片中破坏细胞并纯化核酸的方法和装置。该方法包括用激光束照射芯片，其中芯片包括其上沉积有细胞裂解增强金属氧化物层和细胞结合金属氧化物层的固体支持物。

3. 发明授权

US08658359B2 Method and apparatus for disrupting cells and purifying nucleic acid using a single chip 有权
标题翻译：使用单个芯片破坏细胞并纯化核酸的方法和装置
公开(公告)号：US08658359B2
公开(公告)日：2014-02-25
申请号：US13079926
申请日：2011-04-05
申请人： In-ho Lee , Jun-hong Min , Young-rok Kim , Jae-ho You , Chang-eun Yoo , Jeong-gun Lee , Ki-woong Han
发明人： In-ho Lee , Jun-hong Min , Young-rok Kim , Jae-ho You , Chang-eun Yoo , Jeong-gun Lee , Ki-woong Han
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/00
CPC分类号： C12Q1/68 , C12M47/06 , C12N15/1006
摘要： Provided herein is a method and apparatus for disrupting cells and purifying nucleic acids in a single chip. The method comprises irradiating a chip with a laser beam, wherein the chip comprises a solid support on which a cell lysis enhancing metal oxide layer, and a cell binding metal oxide layer have been deposited.
摘要翻译：本文提供了在单个芯片中破坏细胞并纯化核酸的方法和装置。该方法包括用激光束照射芯片，其中芯片包括其上沉积有细胞裂解增强金属氧化物层和细胞结合金属氧化物层的固体支持物。

4. 发明授权

US07955801B2 Method and apparatus for disrupting cells and purifying nucleic acid using a single chip 有权
标题翻译：使用单个芯片破坏细胞并纯化核酸的方法和装置
公开(公告)号：US07955801B2
公开(公告)日：2011-06-07
申请号：US11639794
申请日：2006-12-15
申请人： In-ho Lee , Jun-hong Min , Young-rok Kim , Jae-ho You , Chang-eun Yoo , Jeong-gun Lee , Ki-woong Han
发明人： In-ho Lee , Jun-hong Min , Young-rok Kim , Jae-ho You , Chang-eun Yoo , Jeong-gun Lee , Ki-woong Han
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/68 , C12M47/06 , C12N15/1006
摘要： Provided herein is a method and apparatus for disrupting cells and purifying nucleic acids in a single chip. The method comprises irradiating a chip with a laser beam, wherein the chip comprises a solid support on which a cell lysis enhancing metal oxide layer, and a cell binding metal oxide layer have been deposited.
摘要翻译：本文提供了在单个芯片中破坏细胞并纯化核酸的方法和装置。该方法包括用激光束照射芯片，其中芯片包括其上沉积有细胞裂解增强金属氧化物层和细胞结合金属氧化物层的固体支持物。

5. 发明授权

US07641858B2 Apparatus for introducing fluid into microfluidic chip by using centrifugal force, a system including the apparatus, and a method of using the apparatus 有权
标题翻译：用于通过离心力将流体引入微流体芯片的装置，包括该装置的系统以及使用该装置的方法
公开(公告)号：US07641858B2
公开(公告)日：2010-01-05
申请号：US11846794
申请日：2007-08-29
申请人： Young-rok Kim , Jun-hong Min , Kak Namkoong , Kwang-ho Cheong , Chang-eun Yoo , Ki-woong Han , Ki-eun Kim
发明人： Young-rok Kim , Jun-hong Min , Kak Namkoong , Kwang-ho Cheong , Chang-eun Yoo , Ki-woong Han , Ki-eun Kim
IPC分类号： B01L3/14 , B65B3/04
CPC分类号： B01L9/527 , B01L3/5021 , B01L3/5027 , B01L2200/027 , B01L2300/0832 , B01L2400/0409 , C12M23/16
摘要： An apparatus introducing a fluid using a centrifugal force includes an introduction member including a chip receiver and a fluid introduction reservoir, the chip receiver receiving a first part of a microfluidic chip, the first part including an inlet, the fluid introduction reservoir storing a fluid to be introduced to the microfluidic chip, the fluid introduction reservoir having an exit formed to correspond to the inlet of the microfluidic chip received in the chip receiver, and a support member supporting a second part of the microfluidic chip, wherein the microfluidic chip is disposed between the introduction member and the support member, the apparatus is rotatable in a state where the introduction member is closer to a center of rotation than the microfluidic chip, and the fluid is introducible from the fluid introduction reservoir through the inlet into the microfluidic chip due to a centrifugal force generated by rotation.
摘要翻译：使用离心力引入流体的装置包括引入构件，其包括芯片接收器和流体引入储存器，所述芯片接收器接收微流体芯片的第一部分，所述第一部分包括入口，所述流体引入储存器将流体存储到引入到微流体芯片中，流体引入储存器具有形成为对应于接收在芯片接收器中的微流体芯片的入口的出口，以及支撑微流体芯片的第二部分的支撑构件，其中微流体芯片设置在引导构件和支撑构件，该装置可以在导入构件比微流体芯片更靠近旋转中心的状态下旋转，并且流体可以从流体引入储存器通过入口引入到微流体芯片中，这是由于由旋转产生的离心力。

6. 发明申请

US20060134777A1 Microfluidic device comprising electrolysis device for cell lysis and method for electrochemically lysing cells using the same 有权
公开(公告)号：US20060134777A1
公开(公告)日：2006-06-22
申请号：US11304083
申请日：2005-12-15
申请人： Hun-joo Lee , Joon-ho Kim , Chang-eun Yoo , Hee-kyun Lim , Kyu-youn Hwang , Soo-min Ma , Jun-hong Min
发明人： Hun-joo Lee , Joon-ho Kim , Chang-eun Yoo , Hee-kyun Lim , Kyu-youn Hwang , Soo-min Ma , Jun-hong Min
IPC分类号： G01N27/447 , C12M1/33
CPC分类号： C12N1/066 , C12M47/06
摘要： Provided are a microfluidic device including an electrolysis device for cell lysis which includes an anode chamber, a cathode chamber and a separator, in which the separator is installed between the anode chamber and the cathode chamber, the anode chamber includes an inlet and an outlet for an anode chamber solution and an electrode, and the cathode chamber includes an inlet and an outlet for a cathode chamber solution and an electrode, and a method of electrochemically lysing cells using the same.

7. 发明授权

US07598064B2 Microfluidic device comprising electrolysis device for cell lysis and method for electrochemically lysing cells using the same 有权
公开(公告)号：US07598064B2
公开(公告)日：2009-10-06
申请号：US12349273
申请日：2009-01-06
申请人： Hun-joo Lee , Joon-ho Kim , Chang-eun Yoo , Hee-kyun Lim , Kyu-youn Hwang , Soo-min Ma , Jun-hong Min
发明人： Hun-joo Lee , Joon-ho Kim , Chang-eun Yoo , Hee-kyun Lim , Kyu-youn Hwang , Soo-min Ma , Jun-hong Min
IPC分类号： C12N13/00 , C12N1/06 , C12M1/33 , C12M1/34 , C12Q1/70
CPC分类号： C12N1/066 , C12M47/06
摘要： Provided are a microfluidic device including an electrolysis device for cell lysis which includes an anode chamber, a cathode chamber and a separator, in which the separator is installed between the anode chamber and the cathode chamber, the anode chamber includes an inlet and an outlet for an anode chamber solution and an electrode, and the cathode chamber includes an inlet and an outlet for a cathode chamber solution and an electrode, and a method of electrochemically lysing cells using the same.

8. 发明申请

US20090159463A1 MICROFLUIDIC DEVICE COMPRISING ELECTROLYSIS DEVICE FOR CELL LYSIS AND METHOD FOR ELECTROCHEMICALLY LYSING CELLS USING THE SAME 有权
标题翻译：包含用于细胞分析的电解装置的微流体装置和使用其的电化学细胞的方法
公开(公告)号：US20090159463A1
公开(公告)日：2009-06-25
申请号：US12349273
申请日：2009-01-06
申请人： Hun-joo LEE , Joon-ho KIM , Chang-eun YOO , Hee-kyun LIM , Kyu-youn Hwang , Soo-min MA , Jun-hong Min
发明人： Hun-joo LEE , Joon-ho KIM , Chang-eun YOO , Hee-kyun LIM , Kyu-youn Hwang , Soo-min MA , Jun-hong Min
IPC分类号： G01N33/50
CPC分类号： C12N1/066 , C12M47/06
摘要： Provided are a microfluidic device including an electrolysis device for cell lysis which includes an anode chamber, a cathode chamber and a separator, in which the separator is installed between the anode chamber and the cathode chamber, the anode chamber includes an inlet and an outlet for an anode chamber solution and an electrode, and the cathode chamber includes an inlet and an outlet for a cathode chamber solution and an electrode, and a method of electrochemically lysing cells using the same.
摘要翻译：提供了一种微流体装置，其包括用于细胞裂解的电解装置，其包括阳极室，阴极室和隔板，其中隔膜安装在阳极室和阴极室之间，阳极室包括入口和出口阳极室溶液和电极，阴极室包括用于阴极室溶液和电极的入口和出口，以及使用其进行电化学裂解细胞的方法。

9. 发明授权

US07727136B2 Apparatus and method for separating material by size using a rotating drum having multiplex axes of rotation 有权
标题翻译：使用具有多个旋转轴的旋转滚筒分离材料的装置和方法
公开(公告)号：US07727136B2
公开(公告)日：2010-06-01
申请号：US11682673
申请日：2007-03-06
申请人： Young-rok Kim , Kak Namkoong , Jun-hong Min
发明人： Young-rok Kim , Kak Namkoong , Jun-hong Min
IPC分类号： B04B7/12 , B04B13/00
CPC分类号： B04B5/02 , B04B13/00 , G01N15/042 , G01N15/05 , G01N21/07 , G01N33/491
摘要： An apparatus and method for centrifugally separating particles by both weight and size include a rotating drum rotating about a second rotating axis disposed perpendicularly to a first rotating axis, and includes an inlet, at least one rotating plate and an outlet. The inlet injects a test material. The rotating plate extends radially outward toward an inner surface of the rotating drum from the second rotating axis and has one or more configurations of protruded and recessed portions formed on a surface thereof. The rotating plate receives the test material on a surface thereof. The outlet discharges separated test material.
摘要翻译：用于通过重量和尺寸离心分离颗粒的装置和方法包括围绕垂直于第一旋转轴设置的第二旋转轴线旋转的旋转滚筒，并且包括入口，至少一个旋转板和出口。入口注入测试材料。旋转板从第二旋转轴线朝向旋转鼓的内表面径向向外延伸，并且具有形成在其表面上的突出和凹陷部分的一个或多个构造。旋转板在其表面上接收测试材料。出口排出分离的测试材料。

10. 发明授权

US07803539B2 Method of isolating and purifying nucleic acids using immobilized hydrogel or PEG-hydrogel copolymer 失效
标题翻译：使用固定化水凝胶或PEG-水凝胶共聚物分离和纯化核酸的方法
公开(公告)号：US07803539B2
公开(公告)日：2010-09-28
申请号：US11297783
申请日：2005-12-07
申请人： Chang-eun Yoo , Joon-ho Kim , Kyu-youn Hwang , Hun-joo Lee , Hee-kyun Lim , Jun-hong Min
发明人： Chang-eun Yoo , Joon-ho Kim , Kyu-youn Hwang , Hun-joo Lee , Hee-kyun Lim , Jun-hong Min
IPC分类号： C12Q1/68 , C12M1/34 , C07H21/00
CPC分类号： C07H21/04
摘要： Provided is a method of isolating and purifying nucleic acids using an immobilized hydrogel or polyethylene glycol (PEG)-hydrogel copolymer. The method includes: immobilizing a functional group-containing hydrogel or PEG-hydrogel copolymer on a substrate; adding a mixed sample solution containing a salt and nucleic acids to the hydrogel- or PEG-hydrogel copolymer-immobilized substrate to bind the nucleic acids to the hydrogel or the PEG-hydrogel copolymer; washing the nucleic acid-bound hydrogel or PEG-hydrogel copolymer; and eluting the nucleic acids from the hydrogel or the PEG-hydrogel copolymer using an elution solvent. Therefore, binding and elution of nucleic acids can be performed even with no addition of a separate chemical substance, and an effect on a subsequent process such as PCR can be minimized. Furthermore, the amount and intensity for binding nucleic acids can be adjusted according to PEG concentration, and the presence of a hydrogel compound on a substrate enables patterning.
摘要翻译：提供了使用固定的水凝胶或聚乙二醇（PEG） - 水凝胶共聚物来分离和纯化核酸的方法。该方法包括：将含官能团的水凝胶或PEG-水凝胶共聚物固定在基材上; 将含有盐和核酸的混合样品溶液加入到水凝胶或PEG-水凝胶共聚物固定的底物中，以将核酸与水凝胶或PEG-水凝胶共聚物结合; 洗涤核酸结合的水凝胶或PEG-水凝胶共聚物; 并使用洗脱溶剂从水凝胶或PEG-水凝胶共聚物中洗脱核酸。因此，即使没有添加单独的化学物质也可以进行核酸的结合和洗脱，并且可以最小化对后续过程（例如PCR）的影响。此外，可以根据PEG浓度调节结合核酸的量和强度，并且在基材上存在水凝胶化合物能够进行图案化。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式