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1. 发明申请

US20060134777A1 Microfluidic device comprising electrolysis device for cell lysis and method for electrochemically lysing cells using the same 有权
公开(公告)号：US20060134777A1
公开(公告)日：2006-06-22
申请号：US11304083
申请日：2005-12-15
申请人： Hun-joo Lee , Joon-ho Kim , Chang-eun Yoo , Hee-kyun Lim , Kyu-youn Hwang , Soo-min Ma , Jun-hong Min
发明人： Hun-joo Lee , Joon-ho Kim , Chang-eun Yoo , Hee-kyun Lim , Kyu-youn Hwang , Soo-min Ma , Jun-hong Min
IPC分类号： G01N27/447 , C12M1/33
CPC分类号： C12N1/066 , C12M47/06
摘要： Provided are a microfluidic device including an electrolysis device for cell lysis which includes an anode chamber, a cathode chamber and a separator, in which the separator is installed between the anode chamber and the cathode chamber, the anode chamber includes an inlet and an outlet for an anode chamber solution and an electrode, and the cathode chamber includes an inlet and an outlet for a cathode chamber solution and an electrode, and a method of electrochemically lysing cells using the same.

2. 发明授权

US07598064B2 Microfluidic device comprising electrolysis device for cell lysis and method for electrochemically lysing cells using the same 有权
公开(公告)号：US07598064B2
公开(公告)日：2009-10-06
申请号：US12349273
申请日：2009-01-06
申请人： Hun-joo Lee , Joon-ho Kim , Chang-eun Yoo , Hee-kyun Lim , Kyu-youn Hwang , Soo-min Ma , Jun-hong Min
发明人： Hun-joo Lee , Joon-ho Kim , Chang-eun Yoo , Hee-kyun Lim , Kyu-youn Hwang , Soo-min Ma , Jun-hong Min
IPC分类号： C12N13/00 , C12N1/06 , C12M1/33 , C12M1/34 , C12Q1/70
CPC分类号： C12N1/066 , C12M47/06
摘要： Provided are a microfluidic device including an electrolysis device for cell lysis which includes an anode chamber, a cathode chamber and a separator, in which the separator is installed between the anode chamber and the cathode chamber, the anode chamber includes an inlet and an outlet for an anode chamber solution and an electrode, and the cathode chamber includes an inlet and an outlet for a cathode chamber solution and an electrode, and a method of electrochemically lysing cells using the same.

3. 发明授权

US07488596B2 Microfluidic device comprising electrolysis device for cell lysis and method for electrochemically lysing cells using the same 有权
标题翻译：微流体装置，其包括用于细胞裂解的电解装置和使用其的电化学裂解细胞的方法
公开(公告)号：US07488596B2
公开(公告)日：2009-02-10
申请号：US11304083
申请日：2005-12-15
申请人： Hun-joo Lee , Joon-ho Kim , Chang-eun Yoo , Hee-kyun Lim , Kyu-youn Hwang , Soo-min Ma , Jun-hong Min
发明人： Hun-joo Lee , Joon-ho Kim , Chang-eun Yoo , Hee-kyun Lim , Kyu-youn Hwang , Soo-min Ma , Jun-hong Min
IPC分类号： C12M1/33 , C12M1/34 , C12Q1/70 , C12N1/06
CPC分类号： C12N1/066 , C12M47/06
摘要： Provided are a microfluidic device including an electrolysis device for cell lysis which includes an anode chamber, a cathode chamber and a separator, in which the separator is installed between the anode chamber and the cathode chamber, the anode chamber includes an inlet and an outlet for an anode chamber solution and an electrode, and the cathode chamber includes an inlet and an outlet for a cathode chamber solution and an electrode, and a method of electrochemically lysing cells using the same.
摘要翻译：提供了一种微流体装置，其包括用于细胞裂解的电解装置，其包括阳极室，阴极室和隔板，其中隔膜安装在阳极室和阴极室之间，阳极室包括入口和出口阳极室溶液和电极，阴极室包括用于阴极室溶液和电极的入口和出口，以及使用其进行电化学裂解细胞的方法。

4. 发明申请

US20060099711A1 Cell lysis method by immobilized metal-ligand complex 失效
标题翻译：固定化金属配体复合物的细胞裂解法
公开(公告)号：US20060099711A1
公开(公告)日：2006-05-11
申请号：US11267005
申请日：2005-11-04
申请人： Chang-eun Yoo , Joon-ho Kim , Kyu-youn Hwang , Hun-joo Lee , Hee-kyun Lim , Soo-min Ma , Nam Huh , Soo-suk Lee
发明人： Chang-eun Yoo , Joon-ho Kim , Kyu-youn Hwang , Hun-joo Lee , Hee-kyun Lim , Soo-min Ma , Nam Huh , Soo-suk Lee
IPC分类号： G01N1/00
CPC分类号： C12Q1/6806 , Y10T436/25 , C12Q2523/308
摘要： Provided is a lysis method for cells or viruses, including: immobilizing a metal-ligand complex on a solid support; and mixing the complex immobilized on the support with a cell or virus solution. According to the lysis method, by immobilizing a chemical on a solid support to perform cell lysis, the dilution problem according to the addition of a cell lysis solution can be resolved and a separate process of removing the chemical is not required so as to reduce the steps upon LOC implementation. In addition, since a variety of solid supports, such as chips, beads, nanoparticles etc. can be used, cell lysis apparatuses of various forms can be fabricated.
摘要翻译：提供了一种细胞或病毒的裂解方法，包括：将固定支持物上的金属 - 配体复合物固定; 并将固定在载体上的复合物与细胞或病毒溶液混合。根据裂解方法，通过将化学物质固定在固体支持体上以进行细胞裂解，可以解决根据细胞溶解溶液的添加的稀释问题，并且不需要单独的除去化学品的方法，以减少 LOC实施步骤。此外，由于可以使用各种固体支持体，例如芯片，珠粒，纳米粒子等，因此可以制造各种形式的细胞裂解装置。

5. 发明授权

US07648838B2 Cell lysis method by immobilized metal-ligand complex 失效
标题翻译：固定化金属配体复合物的细胞裂解法
公开(公告)号：US07648838B2
公开(公告)日：2010-01-19
申请号：US11267005
申请日：2005-11-04
申请人： Chang-eun Yoo , Joon-ho Kim , Kyu-youn Hwang , Hun-joo Lee , Hee-kyun Lim , Soo-min Ma , Nam Huh , Soo-suk Lee
发明人： Chang-eun Yoo , Joon-ho Kim , Kyu-youn Hwang , Hun-joo Lee , Hee-kyun Lim , Soo-min Ma , Nam Huh , Soo-suk Lee
IPC分类号： G01N33/48 , G01N33/20 , G01N1/00
CPC分类号： C12Q1/6806 , Y10T436/25 , C12Q2523/308
摘要： Provided is a lysis method for cells or viruses, including: immobilizing a metal-ligand complex on a solid support; and mixing the complex immobilized on the support with a cell or virus solution. According to the lysis method, by immobilizing a chemical on a solid support to perform cell lysis, the dilution problem according to the addition of a cell lysis solution can be resolved and a separate process of removing the chemical is not required so as to reduce the steps upon LOC implementation. In addition, since a variety of solid supports, such as chips, beads, nanoparticles etc. can be used, cell lysis apparatuses of various forms can be fabricated.
摘要翻译：提供了一种细胞或病毒的裂解方法，包括：将固定支持物上的金属 - 配体复合物固定; 并将固定在载体上的复合物与细胞或病毒溶液混合。根据裂解方法，通过将化学物质固定在固体支持体上以进行细胞裂解，可以解决根据细胞溶解溶液的添加的稀释问题，并且不需要单独的除去化学品的方法，以减少 LOC实施步骤。此外，由于可以使用各种固体支持物，例如芯片，珠粒，纳米粒子等，因此可以制造各种形式的细胞裂解装置。

6. 发明授权

US07803539B2 Method of isolating and purifying nucleic acids using immobilized hydrogel or PEG-hydrogel copolymer 失效
标题翻译：使用固定化水凝胶或PEG-水凝胶共聚物分离和纯化核酸的方法
公开(公告)号：US07803539B2
公开(公告)日：2010-09-28
申请号：US11297783
申请日：2005-12-07
申请人： Chang-eun Yoo , Joon-ho Kim , Kyu-youn Hwang , Hun-joo Lee , Hee-kyun Lim , Jun-hong Min
发明人： Chang-eun Yoo , Joon-ho Kim , Kyu-youn Hwang , Hun-joo Lee , Hee-kyun Lim , Jun-hong Min
IPC分类号： C12Q1/68 , C12M1/34 , C07H21/00
CPC分类号： C07H21/04
摘要： Provided is a method of isolating and purifying nucleic acids using an immobilized hydrogel or polyethylene glycol (PEG)-hydrogel copolymer. The method includes: immobilizing a functional group-containing hydrogel or PEG-hydrogel copolymer on a substrate; adding a mixed sample solution containing a salt and nucleic acids to the hydrogel- or PEG-hydrogel copolymer-immobilized substrate to bind the nucleic acids to the hydrogel or the PEG-hydrogel copolymer; washing the nucleic acid-bound hydrogel or PEG-hydrogel copolymer; and eluting the nucleic acids from the hydrogel or the PEG-hydrogel copolymer using an elution solvent. Therefore, binding and elution of nucleic acids can be performed even with no addition of a separate chemical substance, and an effect on a subsequent process such as PCR can be minimized. Furthermore, the amount and intensity for binding nucleic acids can be adjusted according to PEG concentration, and the presence of a hydrogel compound on a substrate enables patterning.
摘要翻译：提供了使用固定的水凝胶或聚乙二醇（PEG） - 水凝胶共聚物来分离和纯化核酸的方法。该方法包括：将含官能团的水凝胶或PEG-水凝胶共聚物固定在基材上; 将含有盐和核酸的混合样品溶液加入到水凝胶或PEG-水凝胶共聚物固定的底物中，以将核酸与水凝胶或PEG-水凝胶共聚物结合; 洗涤核酸结合的水凝胶或PEG-水凝胶共聚物; 并使用洗脱溶剂从水凝胶或PEG-水凝胶共聚物中洗脱核酸。因此，即使没有添加单独的化学物质也可以进行核酸的结合和洗脱，并且可以最小化对后续过程（例如PCR）的影响。此外，可以根据PEG浓度调节结合核酸的量和强度，并且在基材上存在水凝胶化合物能够进行图案化。

7. 发明申请

US20060134675A1 Method of isolating and purifying nucleic acids using immobilized hydrogel or PEG-hydrogel copolymer 失效
标题翻译：使用固定化水凝胶或PEG-水凝胶共聚物分离和纯化核酸的方法
公开(公告)号：US20060134675A1
公开(公告)日：2006-06-22
申请号：US11297783
申请日：2005-12-07
申请人： Chang-eun Yoo , Joon-ho Kim , Kyu-youn Hwang , Hun-joo Lee , Hee-kyun Lim , Jun-hong Min
发明人： Chang-eun Yoo , Joon-ho Kim , Kyu-youn Hwang , Hun-joo Lee , Hee-kyun Lim , Jun-hong Min
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C07H21/04
摘要： Provided is a method of isolating and purifying nucleic acids using an immobilized hydrogel or polyethylene glycol (PEG)-hydrogel copolymer. The method includes: immobilizing a functional group-containing hydrogel or PEG-hydrogel copolymer on a substrate; adding a mixed sample solution containing a salt and nucleic acids to the hydrogel- or PEG-hydrogel copolymer-immobilized substrate to bind the nucleic acids to the hydrogel or the PEG-hydrogel copolymer; washing the nucleic acid-bound hydrogel or PEG-hydrogel copolymer; and eluting the nucleic acids from the hydrogel or the PEG-hydrogel copolymer using an elution solvent. Therefore, binding and elution of nucleic acids can be performed even with no addition of a separate chemical substance, and an effect on a subsequent process such as PCR can be minimized. Furthermore, the amount and intensity for binding nucleic acids can be adjusted according to PEG concentration, and the presence of a hydrogel compound on a substrate enables patterning.
摘要翻译：提供了使用固定的水凝胶或聚乙二醇（PEG） - 水凝胶共聚物来分离和纯化核酸的方法。该方法包括：将含官能团的水凝胶或PEG-水凝胶共聚物固定在基材上; 将含有盐和核酸的混合样品溶液加入到水凝胶或PEG-水凝胶共聚物固定的底物中，以将核酸与水凝胶或PEG-水凝胶共聚物结合; 洗涤核酸结合的水凝胶或PEG-水凝胶共聚物; 并使用洗脱溶剂从水凝胶或PEG-水凝胶共聚物中洗脱核酸。因此，即使没有添加单独的化学物质也可以进行核酸的结合和洗脱，并且可以最小化对后续过程（例如PCR）的影响。此外，可以根据PEG浓度调节结合核酸的量和强度，并且在基材上存在水凝胶化合物能够进行图案化。

8. 发明授权

US07695678B2 Method of isolating nucleic acid using material positively charged at first pH and containing amino group and carboxyl group 有权
标题翻译：使用在第一个pH下带正电并含有氨基和羧基的物质分离核酸的方法
公开(公告)号：US07695678B2
公开(公告)日：2010-04-13
申请号：US12141894
申请日：2008-06-18
申请人： Kyu-youn Hwang , Joon-ho Kim , Chang-eun Yoo , Hun-joo Lee , Hee-kyun Lim , Sung-yung Jeong , Jeo-young Shim
发明人： Kyu-youn Hwang , Joon-ho Kim , Chang-eun Yoo , Hun-joo Lee , Hee-kyun Lim , Sung-yung Jeong , Jeo-young Shim
IPC分类号： G01N15/06 , A01N61/00 , C12Q1/68 , G01N33/53 , C07H21/04
CPC分类号： C12N15/1006 , B01D15/361 , B01D15/364 , B01J20/3285 , B01J43/00 , C12Q1/6806 , C12Q2527/119 , C12Q2523/308
摘要： A method of isolating nucleic acid from a sample containing nucleic acid is provided. The method includes contacting the sample with a bifunctional material that contains an amino group and a carboxyl group and is positively charged at a first pH to allow binding of the nucleic acid to the bifunctional material; and extracting the nucleic acid at a second pH higher than the first pH from the complex.
摘要翻译：提供了从含有核酸的样品中分离核酸的方法。该方法包括使样品与含有氨基和羧基的双官能材料接触并在第一pH下带正电荷以允许核酸与双功能材料的结合; 以及从所述络合物中提取比所述第一pH高的第二pH的所述核酸。

9. 发明授权

US07402390B2 Method of isolating nucleic acid using material positively charged at first pH and containing amino group and carboxyl group 有权
标题翻译：使用在第一个pH下带正电并含有氨基和羧基的物质分离核酸的方法
公开(公告)号：US07402390B2
公开(公告)日：2008-07-22
申请号：US11317949
申请日：2005-12-23
申请人： Kyu-youn Hwang , Joon-ho Kim , Chang-eun Yoo , Hun-joo Lee , Hee-kyun Lim , Sung-young Jeong , Jeo-young Shim
发明人： Kyu-youn Hwang , Joon-ho Kim , Chang-eun Yoo , Hun-joo Lee , Hee-kyun Lim , Sung-young Jeong , Jeo-young Shim
IPC分类号： C12Q1/68 , G01N33/53 , C07H21/00
CPC分类号： C12N15/1006 , B01D15/361 , B01D15/364 , B01J20/3285 , B01J43/00 , C12Q1/6806 , C12Q2527/119 , C12Q2523/308
摘要： A method of isolating nucleic acid from a sample containing nucleic acid is provided. The method includes contacting the sample with a bifunctional material that contains an amino group and a carboxyl group and is positively charged at a first pH to allow binding of the nucleic acid to the bifunctional material; and extracting the nucleic acid at a second pH higher than the first pH from the complex.
摘要翻译：提供了从含有核酸的样品中分离核酸的方法。该方法包括使样品与含有氨基和羧基的双官能材料接触并在第一pH下带正电荷以允许核酸与双功能材料的结合; 以及从所述络合物中提取比所述第一pH高的第二pH的所述核酸。

10. 发明申请

US20060154284A1 Method of isolating nucleic acid using material positively charged at first pH and containing amino group and carboxyl group 有权
标题翻译：使用在第一个pH下带正电并含有氨基和羧基的物质分离核酸的方法
公开(公告)号：US20060154284A1
公开(公告)日：2006-07-13
申请号：US11317949
申请日：2005-12-23
申请人： Kyu-youn Hwang , Joon-ho Kim , Chang-eun Yoo , Hun-joo Lee , Hee-kyun Lim , Sung-young Jeong , Jeo-young Shim
发明人： Kyu-youn Hwang , Joon-ho Kim , Chang-eun Yoo , Hun-joo Lee , Hee-kyun Lim , Sung-young Jeong , Jeo-young Shim
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12N15/1006 , B01D15/361 , B01D15/364 , B01J20/3285 , B01J43/00 , C12Q1/6806 , C12Q2527/119 , C12Q2523/308
摘要： A method of isolating nucleic acid from a sample containing nucleic acid is provided. The method includes contacting the sample with a bifunctional material that contains an amino group and a carboxyl group and is positively charged at a first pH to allow binding of the nucleic acid to the bifunctional material; and extracting the nucleic acid at a second pH higher than the first pH from the complex.
摘要翻译：提供了从含有核酸的样品中分离核酸的方法。该方法包括使样品与含有氨基和羧基的双官能材料接触并在第一pH下带正电荷以允许核酸与双功能材料的结合; 以及从所述络合物中提取比所述第一pH高的第二pH的所述核酸。

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