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1. 发明授权

US07807360B2 Method and apparatus for concentrating and amplifying nucleic acid in single micro chamber 有权
标题翻译：在单微室中浓缩和扩增核酸的方法和装置
公开(公告)号：US07807360B2
公开(公告)日：2010-10-05
申请号：US11620961
申请日：2007-01-08
申请人： Young-rok Kim , Jun-hong Min , In-ho Lee , Young-sun Lee , Chang-eun Yoo , Ki-woong Han
发明人： Young-rok Kim , Jun-hong Min , In-ho Lee , Young-sun Lee , Chang-eun Yoo , Ki-woong Han
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , B01L3/5027 , B01L3/5088 , C12Q1/686 , C12Q2565/629 , C12Q2537/149 , C12Q2527/125
摘要： A method of sequentially performing concentration and amplification of nucleic acid in a single micro chamber includes: introducing a nucleic acid-containing sample and a solution including a kosmotropic salt to a micro chamber having a hydrophilic interior surface to concentrate the nucleic acid by binding the nucleic acid on the interior surface of the micro chamber; and performing a polymerase chain reaction (PCR) by adding a PCR mixture to the chamber. Since the nucleic acid is reversibly bound to the interior surface of the micro chamber, PCR yield is higher compared with a surface of aluminum oxide in which irreversible binding occurs. In addition, all processes are sequentially performed in a single micro chamber so that the number of samples, consumables, time, and labor for treatment and analysis can be reduced, detection sensitivity can be improved, and risk of sample cross contamination significantly reduced without sample loss by eliminating transporting of the sample. A complete automated system for concentration and amplification of nucleic acid is thus readily provided.
摘要翻译：在单个微室中依次进行核酸的浓缩和扩增的方法包括：将含核酸的样品和包含可染色盐的溶液引入具有亲水性内表面的微室中，以通过结合核酸来浓缩核酸酸在微室的内表面上; 并通过向该室加入PCR混合物进行聚合酶链式反应（PCR）。由于核酸可逆地结合到微室的内表面，与其中发生不可逆结合的氧化铝的表面相比，PCR产率更高。此外，所有过程都在单个微室中顺序进行，以便可以减少样品数量，消耗品，时间和处理和分析的劳动力，可以提高检测灵敏度，并且样品交叉污染的风险在没有样品时显着降低通过消除样品的运输而损失。因此容易提供用于浓缩和扩增核酸的完整的自动化系统。

2. 发明申请

US20070238114A1 Method and apparatus for disrupting cells and purifying nucleic acid using a single chip 有权
标题翻译：使用单个芯片破坏细胞并纯化核酸的方法和装置
公开(公告)号：US20070238114A1
公开(公告)日：2007-10-11
申请号：US11639794
申请日：2006-12-15
申请人： In-ho Lee , Jun-hong Min , Young-rok Kim , Jae-ho You , Chang-eun Yoo , Jeong-gun Lee , Ki-woong Han
发明人： In-ho Lee , Jun-hong Min , Young-rok Kim , Jae-ho You , Chang-eun Yoo , Jeong-gun Lee , Ki-woong Han
IPC分类号： C12Q1/68 , C12N1/08
CPC分类号： C12Q1/68 , C12M47/06 , C12N15/1006
摘要： Provided herein is a method and apparatus for disrupting cells and purifying nucleic acids in a single chip. The method comprises irradiating a chip with a laser beam, wherein the chip comprises a solid support on which a cell lysis enhancing metal oxide layer, and a cell binding metal oxide layer have been deposited.
摘要翻译：本文提供了在单个芯片中破坏细胞并纯化核酸的方法和装置。该方法包括用激光束照射芯片，其中芯片包括其上沉积有细胞裂解增强金属氧化物层和细胞结合金属氧化物层的固体支持物。

3. 发明授权

US08658359B2 Method and apparatus for disrupting cells and purifying nucleic acid using a single chip 有权
标题翻译：使用单个芯片破坏细胞并纯化核酸的方法和装置
公开(公告)号：US08658359B2
公开(公告)日：2014-02-25
申请号：US13079926
申请日：2011-04-05
申请人： In-ho Lee , Jun-hong Min , Young-rok Kim , Jae-ho You , Chang-eun Yoo , Jeong-gun Lee , Ki-woong Han
发明人： In-ho Lee , Jun-hong Min , Young-rok Kim , Jae-ho You , Chang-eun Yoo , Jeong-gun Lee , Ki-woong Han
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/00
CPC分类号： C12Q1/68 , C12M47/06 , C12N15/1006
摘要： Provided herein is a method and apparatus for disrupting cells and purifying nucleic acids in a single chip. The method comprises irradiating a chip with a laser beam, wherein the chip comprises a solid support on which a cell lysis enhancing metal oxide layer, and a cell binding metal oxide layer have been deposited.
摘要翻译：本文提供了在单个芯片中破坏细胞并纯化核酸的方法和装置。该方法包括用激光束照射芯片，其中芯片包括其上沉积有细胞裂解增强金属氧化物层和细胞结合金属氧化物层的固体支持物。

4. 发明授权

US07955801B2 Method and apparatus for disrupting cells and purifying nucleic acid using a single chip 有权
标题翻译：使用单个芯片破坏细胞并纯化核酸的方法和装置
公开(公告)号：US07955801B2
公开(公告)日：2011-06-07
申请号：US11639794
申请日：2006-12-15
申请人： In-ho Lee , Jun-hong Min , Young-rok Kim , Jae-ho You , Chang-eun Yoo , Jeong-gun Lee , Ki-woong Han
发明人： In-ho Lee , Jun-hong Min , Young-rok Kim , Jae-ho You , Chang-eun Yoo , Jeong-gun Lee , Ki-woong Han
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/68 , C12M47/06 , C12N15/1006
摘要： Provided herein is a method and apparatus for disrupting cells and purifying nucleic acids in a single chip. The method comprises irradiating a chip with a laser beam, wherein the chip comprises a solid support on which a cell lysis enhancing metal oxide layer, and a cell binding metal oxide layer have been deposited.
摘要翻译：本文提供了在单个芯片中破坏细胞并纯化核酸的方法和装置。该方法包括用激光束照射芯片，其中芯片包括其上沉积有细胞裂解增强金属氧化物层和细胞结合金属氧化物层的固体支持物。

5. 发明授权

US07641858B2 Apparatus for introducing fluid into microfluidic chip by using centrifugal force, a system including the apparatus, and a method of using the apparatus 有权
标题翻译：用于通过离心力将流体引入微流体芯片的装置，包括该装置的系统以及使用该装置的方法
公开(公告)号：US07641858B2
公开(公告)日：2010-01-05
申请号：US11846794
申请日：2007-08-29
申请人： Young-rok Kim , Jun-hong Min , Kak Namkoong , Kwang-ho Cheong , Chang-eun Yoo , Ki-woong Han , Ki-eun Kim
发明人： Young-rok Kim , Jun-hong Min , Kak Namkoong , Kwang-ho Cheong , Chang-eun Yoo , Ki-woong Han , Ki-eun Kim
IPC分类号： B01L3/14 , B65B3/04
CPC分类号： B01L9/527 , B01L3/5021 , B01L3/5027 , B01L2200/027 , B01L2300/0832 , B01L2400/0409 , C12M23/16
摘要： An apparatus introducing a fluid using a centrifugal force includes an introduction member including a chip receiver and a fluid introduction reservoir, the chip receiver receiving a first part of a microfluidic chip, the first part including an inlet, the fluid introduction reservoir storing a fluid to be introduced to the microfluidic chip, the fluid introduction reservoir having an exit formed to correspond to the inlet of the microfluidic chip received in the chip receiver, and a support member supporting a second part of the microfluidic chip, wherein the microfluidic chip is disposed between the introduction member and the support member, the apparatus is rotatable in a state where the introduction member is closer to a center of rotation than the microfluidic chip, and the fluid is introducible from the fluid introduction reservoir through the inlet into the microfluidic chip due to a centrifugal force generated by rotation.
摘要翻译：使用离心力引入流体的装置包括引入构件，其包括芯片接收器和流体引入储存器，所述芯片接收器接收微流体芯片的第一部分，所述第一部分包括入口，所述流体引入储存器将流体存储到引入到微流体芯片中，流体引入储存器具有形成为对应于接收在芯片接收器中的微流体芯片的入口的出口，以及支撑微流体芯片的第二部分的支撑构件，其中微流体芯片设置在引导构件和支撑构件，该装置可以在导入构件比微流体芯片更靠近旋转中心的状态下旋转，并且流体可以从流体引入储存器通过入口引入到微流体芯片中，这是由于由旋转产生的离心力。

6. 发明申请

US20060223100A1 Method of selectively removing protein from biological samples using chemicals 有权
标题翻译：使用化学物质从生物样品中选择性去除蛋白质的方法
公开(公告)号：US20060223100A1
公开(公告)日：2006-10-05
申请号：US11396761
申请日：2006-04-03
申请人： Myo-yong Lee , Ki-woong Han
发明人： Myo-yong Lee , Ki-woong Han
IPC分类号： C07H21/04 , C12Q1/68
CPC分类号： C07K1/36 , C07K1/20 , C12Q1/6806 , C12Q2527/125
摘要： Provided is a method of removing protein while not removing nucleic acids from a biological sample containing protein, the method including: adding a compound of formula I below and a protein nucleating agent to the biological sample containing protein: where at least two of R1, R2, and R3 substituents are substituted or unsubstituted C1-C6 alkyl groups and the other substituent is a hydrogen atom, and a is an integer of 1 to 6; treating the resultant mixture with a hydrophobic surface material in order to obtain a protein-free mixture; and separating the protein-free mixture from the hydrophobic surface material to which the protein is bound. By using the method, the protein can be selectively, effectively removed from the biological sample containing the protein while a nucleic acid is maintained in the sample.
摘要翻译：提供一种除去蛋白质而不从含有生物样品的蛋白质中除去核酸的方法，该方法包括：将下面的式I化合物和蛋白质成核剂加入到含有蛋白质的生物样品中：其中至少两个R R 1，R 2，R 3和R 3取代基是取代或未取代的C 1 -C 6烷基，而另一个取代基是氢原子，a是 1〜6的整数; 用疏水性表面材料处理所得混合物以获得无蛋白质的混合物; 并将蛋白质与蛋白质结合的疏水表面材料分离出来。通过使用该方法，可以在将样品中保持核酸的同时，从含有蛋白质的生物样品中有效地除去蛋白质。

7. 发明授权

US07435811B2 Method for preferentially removing protein over nucleic acids using physical as well as chemical means of removal of the protein 有权
标题翻译：使用物理以及去除蛋白质的化学方法优先去除蛋白质的方法
公开(公告)号：US07435811B2
公开(公告)日：2008-10-14
申请号：US11396761
申请日：2006-04-03
申请人： Myo-yong Lee , Ki-woong Han
发明人： Myo-yong Lee , Ki-woong Han
IPC分类号： C07H21/00
CPC分类号： C07K1/36 , C07K1/20 , C12Q1/6806 , C12Q2527/125
摘要： Provided is a method of removing protein while not removing nucleic acids from a biological sample containing protein, the method including: adding a compound of formula I below and a protein nucleating agent to the biological sample containing protein: where at least two of R1, R2, and R3 substituents are substituted or unsubstituted C1-C6 alkyl groups and the other substituent is a hydrogen atom or a substituted or unsubstituted C1-C6 alkyl group, a is an integer of 1 to 6, and b is 0 or 1, wherein b is 0 when a is not 1; treating the resultant mixture with a hydrophobic surface material in order to obtain a protein-free mixture; and separating the protein-free mixture from the hydrophobic surface material to which the protein is bound. By using the method, the protein can be selectively, effectively removed from the biological sample containing the protein while a nucleic acid is maintained in the sample.
摘要翻译：提供一种除去蛋白质而不从含有生物样品的蛋白质中除去核酸的方法，该方法包括：将下面的式I化合物和蛋白质成核剂加入到含有蛋白质的生物样品中：其中至少两个R R 1，R 2，R 3和R 3取代基是取代或未取代的C 1 -C 6烷基，而另一个取代基是氢原子或取代或未取代的 C1-C6烷基，a为1〜6的整数，b为0或1，a为1时，b为0; 用疏水性表面材料处理所得混合物以获得无蛋白质的混合物; 并将蛋白质与蛋白质结合的疏水表面材料分离出来。通过使用该方法，可以在将样品中保持核酸的同时，从含有蛋白质的生物样品中有效地除去蛋白质。

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