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1. 发明授权

US5351908A Webbing retractor 失效
标题翻译：织带牵开器
公开(公告)号：US5351908A
公开(公告)日：1994-10-04
申请号：US24453
申请日：1993-03-01
申请人： Yoshio Umezawa , Hisao Yamada
发明人： Yoshio Umezawa , Hisao Yamada
IPC分类号： B60R22/36 , B60R22/415 , B65H75/48
CPC分类号： B60R22/415
摘要： A webbing retractor winds-up a webbing by a winding shaft. A lock ring rotates according to movement of the winding shaft. A rotational delay of the lock ring with respect to the winding shaft is generated when the webbing is rapidly drawn-out. The rotational delay of the lock ring causes a lock plate to prevent the webbing from being drawn-out. An engaging member is movable to a separated position where the engaging member is separated from the lock ring, and to an engaging position where the engaging member engages the lock ring to generate rotational delay. Further, the engaging member is urged in one of the direction of the separated position and the direction of the engaging position when the engaging member is separated from a neutral position disposed at an intermediate portion between the separated position and the engaging position. A moving member moves with the winding shaft and rotates. A guide member in the moving member guides the engaging member to an engaging waiting position positioned on an engaging position side with respect to a neutral position and the engaging member disengages the lock ring by moving the moving member according to movement of the winding shaft to rotate as the webbing is drawn-out. The guide member guides the engaging member to the engaging position by moving the moving member with the winding shaft to rotate as the webbing is retracted in a condition where the engaging member is positioned at the engaging waiting position.
摘要翻译：织带卷收器通过卷绕轴卷绕织带。锁环根据卷轴的运动而旋转。当织带被快速拉出时，产生相对于卷轴的锁定环的旋转延迟。锁定环的旋转延迟导致锁定板防止织带被拉出。接合构件可移动到接合构件与锁定环分离的分离位置以及接合构件接合锁定环以产生旋转延迟的接合位置。此外，当接合构件与设置在分离位置和接合位置之间的中间部分处的中立位置分离时，接合构件被推动在分离位置的方向和接合位置的方向之一上。移动构件随着卷轴移动并旋转。移动构件中的引导构件将接合构件引导到相对于中立位置定位在接合位置侧的接合等待位置，并且接合构件通过沿着卷绕轴的移动移动移动构件而旋转锁定环以旋转当织带被拉出时。当接合构件位于接合等待位置的状态下，引导构件通过使具有卷绕轴的移动构件移动而使接合构件引导到接合位置，以随着织带回缩而旋转。

2. 发明授权

US08658777B2 Activated protease indicator 失效
标题翻译：活化蛋白酶指示剂
公开(公告)号：US08658777B2
公开(公告)日：2014-02-25
申请号：US12451341
申请日：2008-05-09
申请人： Yoshio Umezawa , Takeaki Ozawa , Akira Kanno
发明人： Yoshio Umezawa , Takeaki Ozawa , Akira Kanno
IPC分类号： C07K1/00
CPC分类号： C12Q1/37 , C07K2319/50 , C12N9/0069 , C12Q1/66 , G01N2500/04
摘要： It is an object of the invention to provide novel approaches capable of detecting activated protease and also detecting protease activation on real time at a high sensitivity in a noninvasive manner. By the method for detecting activated protease of the invention, an indicator in a circular form comprising the C-half fragment of luciferase (Luc-C) and the N-half fragment of luciferase (Luc-N) linked together through a substrate peptide for a protease is introduced in an in vitro assay system or in cells. Upon digestion of the substrate peptide by the protease, Luc-N and Luc-C together reconstructs active luciferase, so that the activated protease can be detected by assaying the luminescence signal from the luciferase.
摘要翻译：本发明的目的是提供能够检测活化的蛋白酶的新方法，并且以非侵入性的方式以高灵敏度实时检测蛋白酶活化。通过检测本发明的活化蛋白酶的方法，包括荧光素酶（Luc-C）的C-half片段和荧光素酶（Luc-N）的N-半片段通过底物肽连接在一起的圆形形式的指示剂，用于在体外测定系统或细胞中引入蛋白酶。在通过蛋白酶消化底物肽时，Luc-N和Luc-C一起重构活性荧光素酶，从而可以通过测定荧光素酶的发光信号来检测活化的蛋白酶。

3. 发明授权

US07425430B2 Probe for visualizing phosphorylation/dephosphorylation of protein and method of detecting and quantifying phosphorylation/dephosphorylation of protein 失效
标题翻译：用于可视化蛋白质的磷酸化/去磷酸化的探针和检测和定量蛋白质的磷酸化/去磷酸化的方法
公开(公告)号：US07425430B2
公开(公告)日：2008-09-16
申请号：US10296313
申请日：2001-03-23
申请人： Yoshio Umezawa , Moritoshi Sato , Takeaki Ozawa
发明人： Yoshio Umezawa , Moritoshi Sato , Takeaki Ozawa
IPC分类号： C07K14/00 , C12Q1/42
CPC分类号： C12Q1/42 , C12Q1/485 , G01N33/542 , G01N2500/02
摘要： As a versatile method of detecting and assaying intracellular protein phosphorylation and dephosphorylation that enables nondestructive monitoring as well as spatial and temporal analysis for living cells, animal bodies, plant bodies and the like, a probe for imaging protein phosphorylation and dephosphorylation, which comprises a tandem fusion unit composed of a substrate domain that contains a phosphorylation and dephosphorylation site, a linker sequence and a phosphorylation recognition domain, interposed between a donor chromophore and an acceptor chromophore that cause fluorescence resonance energy transfer, is used.
摘要翻译：作为检测和测定细胞内蛋白磷酸化和脱磷酸化的多功能方法，其能够对活细胞，动物体，植物体等进行非破坏性监测以及空间和时间分析，用于成像蛋白质磷酸化和脱磷酸化的探针，其包含串联使用由包含磷酸化和去磷酸化位点的底物结构域，接头序列和磷酸化识别结构域组成的融合单元，其插入供体生色团和引起荧光共振能量转移的受体发色团之间。

4. 发明授权

US07286098B2 Circular polarization antenna and composite antenna including this antenna 有权
标题翻译：包括该天线的圆极化天线和复合天线
公开(公告)号：US07286098B2
公开(公告)日：2007-10-23
申请号：US10929758
申请日：2004-08-30
申请人： Kazushige Ogino , Yoshio Umezawa , Kazuo Takayama , Koji Nagao , Katsuhiro Tsuruta
发明人： Kazushige Ogino , Yoshio Umezawa , Kazuo Takayama , Koji Nagao , Katsuhiro Tsuruta
IPC分类号： H01Q19/00 , H01Q19/10 , H01Q1/38 , H01Q11/12 , H01Q1/32
CPC分类号： H01Q7/00 , H01Q1/1271 , H01Q9/30 , H01Q21/24 , H01Q21/28
摘要： When configuring a film antenna for receiving a circular polarized wave, at least one loop antenna is formed on a transparent plastic film and, at the same time, a non-powered element constituted by a wire-shaped conductor independent from the antenna conductor configuring the loop is arranged near this loop antenna. The non-powered element arranged on the side of the loop antenna is configured by a first part and a second part. The first part is made close to the loop antenna in a substantially parallel state. When a monopole antenna is used in place of the loop antenna, by combining this with a wire-shaped conductor orthogonal to this, it becomes possible to receive a circular polarized wave by a configuration providing a power transfer part between the two. It is also possible to configure a composite antenna by mounting another antenna on the transparent plastic film. This antenna can be used as an antenna of a navigation system.
摘要翻译：当配置用于接收圆偏振波的胶片天线时，在透明塑料膜上形成至少一个环形天线，并且同时由非导电元件构成，该非电源元件由独立于配置天线导体的天线导体的线状导体构成环路布置在该环形天线附近。布置在环形天线侧的非供电元件由第一部分和第二部分构成。第一部分在基本平行的状态下靠近环形天线。当使用单极天线来代替环形天线时，通过将其与与其垂直的线状导体组合，可以通过在两者之间提供功率传递部分的结构来接收圆偏振波。还可以通过在透明塑料膜上安装另一个天线来配置复合天线。该天线可以用作导航系统的天线。

5. 发明授权

US07169614B2 Electrochemical detection method of complementarity to nucleic acid bases 有权
标题翻译：与核酸碱基互补的电化学检测方法
公开(公告)号：US07169614B2
公开(公告)日：2007-01-30
申请号：US10471657
申请日：2001-03-13
申请人： Yoshio Umezawa , Hiroshi Aoki
发明人： Yoshio Umezawa , Hiroshi Aoki
IPC分类号： G01N33/00 , C12M3/00 , C07H21/04 , C02F1/00 , B32B27/04 , B01D59/42
CPC分类号： C12Q1/6825 , G01N33/5438 , Y10T436/143333
摘要： A convenient and commonly applicable method for the specific detection of a nucleic acid with an arbitrary sequence is provide. This method comprises attaching at least a nucleic acid single strand to an electrode, bringing the thus-obtained modified electrode in contact with a solution containing the analyte single-stranded nucleic acid, and measuring the redox reaction of the redox marker.
摘要翻译：提供了用于特定检测具有任意序列的核酸的方便且常用的方法。该方法包括将至少一个核酸单链连接到电极，使由此获得的修饰电极与含有分析物单链核酸的溶液接触，并测量氧化还原标记的氧化还原反应。

6. 发明申请

US20070022484A1 Probes for analyzing interaction between proteins and method of analyzing interaction between proteins using the same 审中-公开
标题翻译：用于分析蛋白质之间的相互作用的探针和分析蛋白质之间相互作用的方法
公开(公告)号：US20070022484A1
公开(公告)日：2007-01-25
申请号：US10557992
申请日：2004-05-20
申请人： Yoshio Umezawa , Asami Kaihara , Takeaki Ozawa , Moritoshi Sato
发明人： Yoshio Umezawa , Asami Kaihara , Takeaki Ozawa , Moritoshi Sato
IPC分类号： A01K67/027 , C12Q1/66
CPC分类号： C12Q1/66 , A01K2217/05 , C07K2319/41 , C07K2319/43 , C07K2319/70 , C07K2319/72 , C12N9/0069 , G01N33/542
摘要： The present invention provides a pair of probes for analyzing protein-protein interactions, which comprises a probe A containing at least an N-terminal half polypeptide of split Renilla luciferase, and a probe B containing at least the remaining C-terminal half polypeptide of split Renilla luciferase.
摘要翻译：本发明提供了一对用于分析蛋白质 - 蛋白质相互作用的探针，其包含至少包含分裂的海肾荧光素酶的N-末端半多肽的探针A和至少含有分裂的C末端半多肽的探针B 海肾萤光素酶。

7. 发明授权

US08124424B2 Single molecule-format bioluminescent probe 失效
标题翻译：单分子格式生物发光探针
公开(公告)号：US08124424B2
公开(公告)日：2012-02-28
申请号：US12448881
申请日：2008-01-15
申请人： Yoshio Umezawa , Moritoshi Sato , Hiroaki Tao , SungBae Kim
发明人： Yoshio Umezawa , Moritoshi Sato , Hiroaki Tao , SungBae Kim
IPC分类号： G01N33/566 , G01N33/53 , G01N33/567 , C12Q1/66 , C07K1/00 , C12N15/00 , C12N5/00
CPC分类号： G01N33/542
摘要： The claimed invention comprises a single molecule-format bioluminescent probe for detecting a target-specific ligand in a living cell, which comprises, a ligand-binding molecule of which conformation is changed upon binding to the ligand, wherein the ligand-binding molecule comprises a ligand-binding domain (LBD) of a nuclear receptor and an LBD-interacting domain that is a co-activator peptide of said nuclear receptor, and an N-terminal polypeptide and a C-terminal polypeptide of a click beetle luciferase (N-CBLuc and C-CBLuc), which flank each end of the ligand-binding molecule, respectively, wherein the N-CBLuc and the C-CBLuc self-complement to generate a luminescent signal only upon binding of the ligand to the ligand-binding molecule.
摘要翻译：所要求保护的发明包括用于检测活细胞中的靶特异性配体的单分子形式的生物发光探针，其包含在与配体结合时构象改变的配体结合分子，其中配体结合分子包含核受体的配体结合结构域（LBD）和作为所述核受体的共活化剂肽的LBD相互作用结构域，以及点击甲虫荧光素酶的N末端多肽和C-末端多肽（N-CBLuc 和C-CBLuc），其分别位于配体结合分子的每个末端，其中N-CBLuc和C-CBLuc自补体仅在配体结合配体结合分子时产生发光信号。

8. 发明申请

US20110312014A1 PROBE FOR DETECTION AND QUANTIFICATION OF NITRIC OXIDE, AND METHOD FOR DETECTING AND QUANTIFYING NITRIC OXIDE USING THE SAME 失效
标题翻译：氮氧化物检测和定量检测方法及使用该方法检测和定量氮氧化物的方法
公开(公告)号：US20110312014A1
公开(公告)日：2011-12-22
申请号：US13103317
申请日：2011-05-09
申请人： Yoshio Umezawa , Moritoshi Sato
发明人： Yoshio Umezawa , Moritoshi Sato
IPC分类号： C12Q1/25 , C12Q1/02
CPC分类号： C12Q1/527
摘要： Probe 1 for detection and quantification of nitric oxide, which comprises two subunits 21 and 22 of soluble guanylate cyclase 2 and cGMP-visualization probes 3 respectively connected with each subunit, wherein the cGMP-visualization probe generates signal upon recognizing guanosine 3′,5′-cyclic monophospate.
摘要翻译：用于检测和定量一氧化氮的探针1，其包含分别与每个亚基连接的可溶性鸟苷酸环化酶2和cGMP可视化探针3的两个亚基21和22，其中cGMP可视化探针在识别鸟苷3'，5'时产生信号，环状单磷酸盐

9. 发明授权

US07623080B2 Loop antenna attached to rear window of vehicle 失效
标题翻译：环形天线连接到车辆的后窗
公开(公告)号：US07623080B2
公开(公告)日：2009-11-24
申请号：US11606500
申请日：2006-11-28
申请人： Kazushige Ogino , Yoshio Umezawa , Katsuhiro Tsuruta , Akihiro Fujiwara
发明人： Kazushige Ogino , Yoshio Umezawa , Katsuhiro Tsuruta , Akihiro Fujiwara
IPC分类号： H01Q1/32 , H01Q1/02
CPC分类号： H01Q7/00 , H01Q1/1271
摘要： A loop antenna able to be mounted on the rear window of a vehicle provided with a defogger, that is, a loop antenna to be mounted on a rear window provided with a defogger having electrodes arranged in the vertical direction at the two ends and a plurality of electrical heating wires bridging the electrodes in the horizontal direction, wherein an antenna element forming a loop is made polygonal in shape, two power feed terminals of the loop antenna are provided at positions a predetermined distance away from the midpoint of the bottom of the antenna element, constituted by one side, in the vertical direction, a distance between the power feed terminals and bottom is formed smaller than the distance between adjoining electrical heating wires of the defogger, and the loop antenna is mounted on the rear window between the adjoining electrical heating wires. The polygonal shape may be a triangular shape.
摘要翻译：一种环形天线，其能够安装在设置有除雾器的车辆的后窗上，即，安装在后窗上的环形天线，该后部窗设置有在两端具有沿垂直方向排列的电极的除雾器，在水平方向上桥接电极的电加热线，其中形成环的天线元件形状为多边形，环形天线的两个馈电端子设置在远离天线底部预定距离的位置元件由一侧构成，在垂直方向上，供电端子和底部之间的距离形成为小于除雾器的相邻电热丝之间的距离，并且环形天线安装在相邻电气加热线。多边形可以是三角形。

10. 发明申请

US20060265764A1 Probe for detecting and quantifying lipid second messenger and method of detecting and quantifying lipid second messenger using the same 失效
公开(公告)号：US20060265764A1
公开(公告)日：2006-11-23
申请号：US10554194
申请日：2004-03-15
申请人： Yoshio Umezawa , Moritoshi Sato
发明人： Yoshio Umezawa , Moritoshi Sato
IPC分类号： A01K67/00 , C12Q1/68 , G01N33/567
CPC分类号： G01N33/542 , G01N33/92 , G01N2333/4701 , Y10T436/143333
摘要： The present invention provides probes for detection and quantification of a lipid second messenger, which comprises: a polypeptide specifically bound to the lipid second messenger; two chromophores respectively having different fluorescence wavelengths, wherein each of the chromophores is linked to each end of the polypeptide through a rigid linker sequence; and a membrane localization sequence linked to one of the chromophores through a rigid linker sequence. According to the present invention, it is now possible to quantitatively detect when and in which site of a living cell the lipid second messengers are produced.

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