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1. 发明授权

US07329494B2 Method of detection and quantification of cGMP by using a cGMP-visualizing probe 失效
标题翻译：通过使用cGMP可视化探针检测和定量cGMP的方法
公开(公告)号：US07329494B2
公开(公告)日：2008-02-12
申请号：US11136430
申请日：2005-05-25
申请人： Yoshio Umezawa , Moritoshi Sato , Takeaki Ozawa
发明人： Yoshio Umezawa , Moritoshi Sato , Takeaki Ozawa
IPC分类号： C12Q1/68 , C12Q1/48
CPC分类号： C12Q1/48 , C12Q1/44 , C12Q1/485 , G01N33/542 , G01N33/5735 , G01N33/582 , G01N2500/02 , Y10T436/143333
摘要： As a cGMP-visualizing probe capable of detecting and quantifying cGMP easily and accurately even in vivo and for a method of detecting and quantifying cGMP by using the same, a cGMP-visualizing probe comprising a polypeptide, which binds specifically to cGMP, and two chromophores having different fluorescence wavelengths each linked respectively to the two terminals of said polypeptide is provided.
摘要翻译：作为能够即使在体内也容易且准确地检测和定量cGMP的cGMP可视化探针，以及通过使用其检测和定量cGMP的方法，可以使用包含特异性结合cGMP的多肽和两种发色团的cGMP可视化探针提供了各自分别连接到所述多肽的两个末端的不同荧光波长。

2. 发明授权

US06924119B2 cGMP-visualizing probe and a method of detecting and quantifying of cGMP by using the same 失效
标题翻译： cGMP可视化探针和通过使用其检测和定量cGMP的方法
公开(公告)号：US06924119B2
公开(公告)日：2005-08-02
申请号：US10070131
申请日：2001-06-29
申请人： Yoshio Umezawa , Moritoshi Sato , Takeaki Ozawa
发明人： Yoshio Umezawa , Moritoshi Sato , Takeaki Ozawa
IPC分类号： A01K67/027 , C07K14/47 , C07K19/00 , C12N5/10 , C12N9/12 , C12N15/09 , C12Q1/02 , C12Q1/44 , C12Q1/48 , C12R1/91 , G01N21/64 , G01N21/78 , G01N33/15 , G01N33/50 , G01N33/542 , G01N33/573 , G01N33/58 , G01N33/52 , G01N33/566
CPC分类号： C12Q1/48 , C12Q1/44 , C12Q1/485 , G01N33/542 , G01N33/5735 , G01N33/582 , G01N2500/02 , Y10T436/143333
摘要： As a cGMP-visualizing probe capable of detecting and quantifying cGMP easily and accurately even in vivo and for a method of detecting and quantifying cGMP by using the same, a cGMP-visualizing probe comprising a polypeptide, which binds specifically to cGMP, and two chromophores having different fluorescence wavelengths each linked respectively to the two terminals of said polypeptide is provided.
摘要翻译：作为能够即使在体内也容易且准确地检测和定量cGMP的cGMP可视化探针，以及通过使用其检测和定量cGMP的方法，可以使用包含特异性结合cGMP的多肽和两种发色团的cGMP可视化探针提供了各自分别连接到所述多肽的两个末端的不同荧光波长。

3. 发明申请

US20050049396A1 Probe for visualizing phosphorylation/dephosphorylation of protein and method of detecting and quantifying phosphorylation/dephosphorylation of protein 失效
标题翻译：用于可视化蛋白质的磷酸化/去磷酸化的探针和检测和定量蛋白质的磷酸化/去磷酸化的方法
公开(公告)号：US20050049396A1
公开(公告)日：2005-03-03
申请号：US10296313
申请日：2001-03-23
申请人： Yoshio Umezawa , Moritoshi Sato , Takeaki Ozawa
发明人： Yoshio Umezawa , Moritoshi Sato , Takeaki Ozawa
IPC分类号： C12Q1/42 , C12Q1/48 , G01N21/64 , G01N33/542 , G01N33/58 , C07K14/435
CPC分类号： C12Q1/42 , C12Q1/485 , G01N33/542 , G01N2500/02
摘要： As a versatile method of detecting and assaying intracellular protein phosphorylation and dephosphorylation that enables nondestructive monitoring as well as spatial and temporal analysis for living cells, animal bodies, plant bodies and the like, a probe for imaging protein phosphorylation and dephosphorylation, which comprises a tandem fusion unit composed of a substrate domain that contains a phosphorylation and dephosphorylation site, a linker sequence and a phosphorylation recognition domain, interposed between a donor chromophore and an acceptor chromophore that cause fluorescence resonance energy transfer, is used.
摘要翻译：作为检测和测定细胞内蛋白磷酸化和脱磷酸化的多功能方法，其能够对活细胞，动物体，植物体等进行非破坏性监测以及空间和时间分析，用于成像蛋白质磷酸化和脱磷酸化的探针，其包含串联使用由包含磷酸化和去磷酸化位点的底物结构域，接头序列和磷酸化识别结构域组成的融合单元，其插入供体生色团和引起荧光共振能量转移的受体发色团之间。

4. 发明授权

US07425430B2 Probe for visualizing phosphorylation/dephosphorylation of protein and method of detecting and quantifying phosphorylation/dephosphorylation of protein 失效
标题翻译：用于可视化蛋白质的磷酸化/去磷酸化的探针和检测和定量蛋白质的磷酸化/去磷酸化的方法
公开(公告)号：US07425430B2
公开(公告)日：2008-09-16
申请号：US10296313
申请日：2001-03-23
申请人： Yoshio Umezawa , Moritoshi Sato , Takeaki Ozawa
发明人： Yoshio Umezawa , Moritoshi Sato , Takeaki Ozawa
IPC分类号： C07K14/00 , C12Q1/42
CPC分类号： C12Q1/42 , C12Q1/485 , G01N33/542 , G01N2500/02
摘要： As a versatile method of detecting and assaying intracellular protein phosphorylation and dephosphorylation that enables nondestructive monitoring as well as spatial and temporal analysis for living cells, animal bodies, plant bodies and the like, a probe for imaging protein phosphorylation and dephosphorylation, which comprises a tandem fusion unit composed of a substrate domain that contains a phosphorylation and dephosphorylation site, a linker sequence and a phosphorylation recognition domain, interposed between a donor chromophore and an acceptor chromophore that cause fluorescence resonance energy transfer, is used.
摘要翻译：作为检测和测定细胞内蛋白磷酸化和脱磷酸化的多功能方法，其能够对活细胞，动物体，植物体等进行非破坏性监测以及空间和时间分析，用于成像蛋白质磷酸化和脱磷酸化的探针，其包含串联使用由包含磷酸化和去磷酸化位点的底物结构域，接头序列和磷酸化识别结构域组成的融合单元，其插入供体生色团和引起荧光共振能量转移的受体发色团之间。

5. 发明申请

US20070022484A1 Probes for analyzing interaction between proteins and method of analyzing interaction between proteins using the same 审中-公开
标题翻译：用于分析蛋白质之间的相互作用的探针和分析蛋白质之间相互作用的方法
公开(公告)号：US20070022484A1
公开(公告)日：2007-01-25
申请号：US10557992
申请日：2004-05-20
申请人： Yoshio Umezawa , Asami Kaihara , Takeaki Ozawa , Moritoshi Sato
发明人： Yoshio Umezawa , Asami Kaihara , Takeaki Ozawa , Moritoshi Sato
IPC分类号： A01K67/027 , C12Q1/66
CPC分类号： C12Q1/66 , A01K2217/05 , C07K2319/41 , C07K2319/43 , C07K2319/70 , C07K2319/72 , C12N9/0069 , G01N33/542
摘要： The present invention provides a pair of probes for analyzing protein-protein interactions, which comprises a probe A containing at least an N-terminal half polypeptide of split Renilla luciferase, and a probe B containing at least the remaining C-terminal half polypeptide of split Renilla luciferase.
摘要翻译：本发明提供了一对用于分析蛋白质 - 蛋白质相互作用的探针，其包含至少包含分裂的海肾荧光素酶的N-末端半多肽的探针A和至少含有分裂的C末端半多肽的探针B 海肾萤光素酶。

6. 发明申请

US20060134655A1 Method of detection and quantification of cGMP by using a cGMP-visualizing probe 失效
标题翻译：通过使用cGMP可视化探针检测和定量cGMP的方法
公开(公告)号：US20060134655A1
公开(公告)日：2006-06-22
申请号：US11136430
申请日：2005-05-25
申请人： Yoshio Umezawa , Moritoshi Sato , Takeaki Ozawa
发明人： Yoshio Umezawa , Moritoshi Sato , Takeaki Ozawa
IPC分类号： C12Q1/68
CPC分类号： C12Q1/48 , C12Q1/44 , C12Q1/485 , G01N33/542 , G01N33/5735 , G01N33/582 , G01N2500/02 , Y10T436/143333
摘要： As a cGMP-visualizing probe capable of detecting and quantifying cGMP easily and accurately even in vivo and for a method of detecting and quantifying cGMP by using the same, a cGMP-visualizing probe comprising a polypeptide, which binds specifically to cGMP, and two chromophores having different fluorescence wavelengths each linked respectively to the two terminals of said polypeptide is provided.
摘要翻译：作为能够即使在体内也容易且准确地检测和定量cGMP的cGMP可视化探针，以及通过使用其检测和定量cGMP的方法，可以使用包含特异性结合cGMP的多肽和两种发色团的cGMP可视化探针提供了各自分别连接到所述多肽的两个末端的不同荧光波长。

7. 发明申请

US20050221412A1 Probes for imaging protein phosphorylation and dephosphorylation and method for detecting and determining protein phosphorylation and dephosphorylation using the same 审中-公开
标题翻译：用于成像蛋白磷酸化和脱磷酸化的探针以及用于检测和测定蛋白磷酸化和去磷酸化的方法
公开(公告)号：US20050221412A1
公开(公告)日：2005-10-06
申请号：US11149169
申请日：2005-06-10
申请人： Yoshio Umezawa , Moritoshi Sato , Takeaki Ozawa
发明人： Yoshio Umezawa , Moritoshi Sato , Takeaki Ozawa
IPC分类号： C12Q1/42 , C12Q1/48 , G01N21/64 , G01N33/542 , G01N33/58
CPC分类号： C12Q1/42 , C12Q1/485 , G01N33/542 , G01N2500/02
摘要： As a versatile method of detecting and assaying intracellular protein phosphorylation and dephosphorylation that enables nondestructive monitoring as well as spatial and temporal analysis for living cells, animal bodies, plant bodies and the like, a probe for imaging protein phosphorylation and dephosphorylation, which comprises a tandem fusion unit composed of a substrate domain that contains a phosphorylation and dephosphorylation site, a linker sequence and a phosphorylation recognition domain, interposed between a donor chromophore and an acceptor chromophore that cause fluorescence resonance energy transfer, is used.
摘要翻译：作为检测和测定细胞内蛋白磷酸化和脱磷酸化的多功能方法，其能够对活细胞，动物体，植物体等进行非破坏性监测以及空间和时间分析，用于成像蛋白质磷酸化和脱磷酸化的探针，其包含串联使用由包含磷酸化和去磷酸化位点的底物结构域，接头序列和磷酸化识别结构域组成的融合单元，其插入供体生色团和引起荧光共振能量转移的受体发色团之间。

8. 发明授权

US08658777B2 Activated protease indicator 失效
标题翻译：活化蛋白酶指示剂
公开(公告)号：US08658777B2
公开(公告)日：2014-02-25
申请号：US12451341
申请日：2008-05-09
申请人： Yoshio Umezawa , Takeaki Ozawa , Akira Kanno
发明人： Yoshio Umezawa , Takeaki Ozawa , Akira Kanno
IPC分类号： C07K1/00
CPC分类号： C12Q1/37 , C07K2319/50 , C12N9/0069 , C12Q1/66 , G01N2500/04
摘要： It is an object of the invention to provide novel approaches capable of detecting activated protease and also detecting protease activation on real time at a high sensitivity in a noninvasive manner. By the method for detecting activated protease of the invention, an indicator in a circular form comprising the C-half fragment of luciferase (Luc-C) and the N-half fragment of luciferase (Luc-N) linked together through a substrate peptide for a protease is introduced in an in vitro assay system or in cells. Upon digestion of the substrate peptide by the protease, Luc-N and Luc-C together reconstructs active luciferase, so that the activated protease can be detected by assaying the luminescence signal from the luciferase.
摘要翻译：本发明的目的是提供能够检测活化的蛋白酶的新方法，并且以非侵入性的方式以高灵敏度实时检测蛋白酶活化。通过检测本发明的活化蛋白酶的方法，包括荧光素酶（Luc-C）的C-half片段和荧光素酶（Luc-N）的N-半片段通过底物肽连接在一起的圆形形式的指示剂，用于在体外测定系统或细胞中引入蛋白酶。在通过蛋白酶消化底物肽时，Luc-N和Luc-C一起重构活性荧光素酶，从而可以通过测定荧光素酶的发光信号来检测活化的蛋白酶。

9. 发明申请

US20070178464A1 Probes for detecting protein nuclear transport and method for detecting and quantifying protein nuclear transport using the same 审中-公开
标题翻译：用于检测蛋白质核转运的探针和用于检测和定量蛋白质核转运的方法
公开(公告)号：US20070178464A1
公开(公告)日：2007-08-02
申请号：US10591990
申请日：2005-03-09
申请人： Yoshio Umezawa , Takeaki Ozawa
发明人： Yoshio Umezawa , Takeaki Ozawa
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： G01N33/5035 , C12N15/62
摘要： A convenient and highly accurate method for detecting protein nuclear transport induced by an endogenous or exogenous substance in local areas of living cells or animals is provided. The method uses a pair of probes for detecting protein nuclear transport, comprising Probe I and Probe II. In Probe I, a protein whose nuclear transport is to be detected or quantified is connected to an N-terminal end or a C-terminal end of a fusion protein [intein-C/reporter protein-C] wherein at least a C-terminal side polypeptide of an intein and a C-terminal side polypeptide of a reporter protein are connected in this order, and in Probe II, a nuclear localization signal is connected to an N-terminal end or a C-terminal end of a fusion protein [reporter protein-N/intein-N] wherein at least the remaining N-terminal side polypeptide of the reporter protein and the remaining N-terminal side polypeptide of the intein are connected in this order.
摘要翻译：提供了一种用于在活细胞或动物的局部区域内检测由内源或外源物质诱导的蛋白质核转运的方法和高度准确的方法。该方法使用一对探针来检测蛋白质核转运，包括探针I和探针II。在探针I中，要检测或定量其核转运的蛋白质与融合蛋白[内含肽C /报道蛋白C]的N-末端或C-末端连接，其中至少一个C-末端报道蛋白的内含肽和C-末端侧多肽的侧面多肽依次连接，在探针II中，核定位信号连接于融合蛋白的N-末端或C末端[ 报告蛋白N /内含肽-N]，其中报道蛋白的至少剩余的N-末端侧多肽和内含肽的其余N-末端侧多肽以此顺序连接。

10. 发明申请

US20100221719A1 Probes for detecting protein nuclear transport and method for detecting and quantifying protein nuclear transport using the same 审中-公开
标题翻译：用于检测蛋白质核转运的探针和用于检测和定量蛋白质核转运的方法
公开(公告)号：US20100221719A1
公开(公告)日：2010-09-02
申请号：US12656138
申请日：2010-01-19
申请人： Yoshio Umezawa , Takeaki Ozawa
发明人： Yoshio Umezawa , Takeaki Ozawa
IPC分类号： C12Q1/68
CPC分类号： G01N33/5035 , C12N15/62
摘要： A convenient and highly accurate method for detecting protein nuclear transport induced by an endogenous or exogenous substance in local areas of living cells or animals is provided. The method uses a pair of probes for detecting protein nuclear transport, comprising Probe I and Probe II. In Probe I, a protein whose nuclear transport is to be detected or quantified is connected to an N-terminal end or a C-terminal end of a fusion protein [intein-C/reporter protein-C] wherein at least a C-terminal side polypeptide of an intein and a C-terminal side polypeptide of a reporter protein are connected in this order, and in Probe II, a nuclear localization signal is connected to an N-terminal end or a C-terminal end of a fusion protein [reporter protein-N/intein-N] wherein at least the remaining N-terminal side polypeptide of the reporter protein and the remaining N-terminal side polypeptide of the intein are connected in this order.
摘要翻译：提供了一种用于在活细胞或动物的局部区域内检测由内源或外源物质诱导的蛋白质核转运的方法和高度准确的方法。该方法使用一对探针来检测蛋白质核转运，包括探针I和探针II。在探针I中，要检测或定量其核转运的蛋白质与融合蛋白[内含肽C /报道蛋白C]的N-末端或C-末端连接，其中至少一个C-末端报道蛋白的内含肽和C-末端侧多肽的侧面多肽依次连接，在探针II中，核定位信号连接于融合蛋白的N-末端或C末端[ 报告蛋白N /内含肽-N]，其中报道蛋白的至少剩余的N-末端侧多肽和内含肽的其余N-末端侧多肽以此顺序连接。

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