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1. 发明授权

US08748117B2 Probe for detection and quantification of nitric oxide, and method for detecting and quantifying nitric oxide using the same 失效
标题翻译：一氧化氮检测和定量检测方法及使用该方法检测和定量一氧化氮
公开(公告)号：US08748117B2
公开(公告)日：2014-06-10
申请号：US13103317
申请日：2011-05-09
申请人： Yoshio Umezawa , Moritoshi Sato
发明人： Yoshio Umezawa , Moritoshi Sato
IPC分类号： G01N33/53
CPC分类号： C12Q1/527
摘要： Probe 1 for detection and quantification of nitric oxide, which comprises two subunits 21 and 22 of soluble guanylate cyclase 2 and cGMP-visualization probes 3 respectively connected with each subunit, wherein the cGMP-visualization probe generates signal upon recognizing guanosine 3′,5′-cyclic monophospate.
摘要翻译：用于检测和定量一氧化氮的探针1，其包含分别与每个亚基连接的可溶性鸟苷酸环化酶2和cGMP可视化探针3的两个亚基21和22，其中cGMP可视化探针在识别鸟苷3'，5'时产生信号，环状单磷酸盐

2. 发明申请

US20090055939A1 Probe for detection and quantification of nitric oxide, and method for detecting and quantifying nitric oxide using the same 审中-公开
标题翻译：一氧化氮检测和定量检测方法及使用该方法检测和定量一氧化氮
公开(公告)号：US20090055939A1
公开(公告)日：2009-02-26
申请号：US11631422
申请日：2005-07-05
申请人： Yoshio Umezawa , Moritoshi Sato
发明人： Yoshio Umezawa , Moritoshi Sato
IPC分类号： C12Q1/68 , G01N33/566
CPC分类号： C12Q1/527
摘要： Probe 1 for detection and quantification of nitric oxide, which, comprises two subunits 21 and 22 of soluble guanylate cyclase 2 and cGMP-visualization probes 3 respectively connected with each subunit, wherein the cGMP-visualization probe generates signal upon recognizing guanosine 3′,5-cyclic monophospate.
摘要翻译：用于检测和定量一氧化氮的探针1，其包含分别与每个亚基连接的可溶性鸟苷酸环化酶2和cGMP可视化探针3的两个亚基21和22，其中cGMP可视化探针在识别鸟苷3'，5 环状单磷酸盐

3. 发明授权

US07329494B2 Method of detection and quantification of cGMP by using a cGMP-visualizing probe 失效
标题翻译：通过使用cGMP可视化探针检测和定量cGMP的方法
公开(公告)号：US07329494B2
公开(公告)日：2008-02-12
申请号：US11136430
申请日：2005-05-25
申请人： Yoshio Umezawa , Moritoshi Sato , Takeaki Ozawa
发明人： Yoshio Umezawa , Moritoshi Sato , Takeaki Ozawa
IPC分类号： C12Q1/68 , C12Q1/48
CPC分类号： C12Q1/48 , C12Q1/44 , C12Q1/485 , G01N33/542 , G01N33/5735 , G01N33/582 , G01N2500/02 , Y10T436/143333
摘要： As a cGMP-visualizing probe capable of detecting and quantifying cGMP easily and accurately even in vivo and for a method of detecting and quantifying cGMP by using the same, a cGMP-visualizing probe comprising a polypeptide, which binds specifically to cGMP, and two chromophores having different fluorescence wavelengths each linked respectively to the two terminals of said polypeptide is provided.
摘要翻译：作为能够即使在体内也容易且准确地检测和定量cGMP的cGMP可视化探针，以及通过使用其检测和定量cGMP的方法，可以使用包含特异性结合cGMP的多肽和两种发色团的cGMP可视化探针提供了各自分别连接到所述多肽的两个末端的不同荧光波长。

4. 发明授权

US08124424B2 Single molecule-format bioluminescent probe 失效
标题翻译：单分子格式生物发光探针
公开(公告)号：US08124424B2
公开(公告)日：2012-02-28
申请号：US12448881
申请日：2008-01-15
申请人： Yoshio Umezawa , Moritoshi Sato , Hiroaki Tao , SungBae Kim
发明人： Yoshio Umezawa , Moritoshi Sato , Hiroaki Tao , SungBae Kim
IPC分类号： G01N33/566 , G01N33/53 , G01N33/567 , C12Q1/66 , C07K1/00 , C12N15/00 , C12N5/00
CPC分类号： G01N33/542
摘要： The claimed invention comprises a single molecule-format bioluminescent probe for detecting a target-specific ligand in a living cell, which comprises, a ligand-binding molecule of which conformation is changed upon binding to the ligand, wherein the ligand-binding molecule comprises a ligand-binding domain (LBD) of a nuclear receptor and an LBD-interacting domain that is a co-activator peptide of said nuclear receptor, and an N-terminal polypeptide and a C-terminal polypeptide of a click beetle luciferase (N-CBLuc and C-CBLuc), which flank each end of the ligand-binding molecule, respectively, wherein the N-CBLuc and the C-CBLuc self-complement to generate a luminescent signal only upon binding of the ligand to the ligand-binding molecule.
摘要翻译：所要求保护的发明包括用于检测活细胞中的靶特异性配体的单分子形式的生物发光探针，其包含在与配体结合时构象改变的配体结合分子，其中配体结合分子包含核受体的配体结合结构域（LBD）和作为所述核受体的共活化剂肽的LBD相互作用结构域，以及点击甲虫荧光素酶的N末端多肽和C-末端多肽（N-CBLuc 和C-CBLuc），其分别位于配体结合分子的每个末端，其中N-CBLuc和C-CBLuc自补体仅在配体结合配体结合分子时产生发光信号。

5. 发明申请

US20110312014A1 PROBE FOR DETECTION AND QUANTIFICATION OF NITRIC OXIDE, AND METHOD FOR DETECTING AND QUANTIFYING NITRIC OXIDE USING THE SAME 失效
标题翻译：氮氧化物检测和定量检测方法及使用该方法检测和定量氮氧化物的方法
公开(公告)号：US20110312014A1
公开(公告)日：2011-12-22
申请号：US13103317
申请日：2011-05-09
申请人： Yoshio Umezawa , Moritoshi Sato
发明人： Yoshio Umezawa , Moritoshi Sato
IPC分类号： C12Q1/25 , C12Q1/02
CPC分类号： C12Q1/527
摘要： Probe 1 for detection and quantification of nitric oxide, which comprises two subunits 21 and 22 of soluble guanylate cyclase 2 and cGMP-visualization probes 3 respectively connected with each subunit, wherein the cGMP-visualization probe generates signal upon recognizing guanosine 3′,5′-cyclic monophospate.
摘要翻译：用于检测和定量一氧化氮的探针1，其包含分别与每个亚基连接的可溶性鸟苷酸环化酶2和cGMP可视化探针3的两个亚基21和22，其中cGMP可视化探针在识别鸟苷3'，5'时产生信号，环状单磷酸盐

6. 发明申请

US20060265764A1 Probe for detecting and quantifying lipid second messenger and method of detecting and quantifying lipid second messenger using the same 失效
公开(公告)号：US20060265764A1
公开(公告)日：2006-11-23
申请号：US10554194
申请日：2004-03-15
申请人： Yoshio Umezawa , Moritoshi Sato
发明人： Yoshio Umezawa , Moritoshi Sato
IPC分类号： A01K67/00 , C12Q1/68 , G01N33/567
CPC分类号： G01N33/542 , G01N33/92 , G01N2333/4701 , Y10T436/143333
摘要： The present invention provides probes for detection and quantification of a lipid second messenger, which comprises: a polypeptide specifically bound to the lipid second messenger; two chromophores respectively having different fluorescence wavelengths, wherein each of the chromophores is linked to each end of the polypeptide through a rigid linker sequence; and a membrane localization sequence linked to one of the chromophores through a rigid linker sequence. According to the present invention, it is now possible to quantitatively detect when and in which site of a living cell the lipid second messengers are produced.

7. 发明授权

US06924119B2 cGMP-visualizing probe and a method of detecting and quantifying of cGMP by using the same 失效
标题翻译： cGMP可视化探针和通过使用其检测和定量cGMP的方法
公开(公告)号：US06924119B2
公开(公告)日：2005-08-02
申请号：US10070131
申请日：2001-06-29
申请人： Yoshio Umezawa , Moritoshi Sato , Takeaki Ozawa
发明人： Yoshio Umezawa , Moritoshi Sato , Takeaki Ozawa
IPC分类号： A01K67/027 , C07K14/47 , C07K19/00 , C12N5/10 , C12N9/12 , C12N15/09 , C12Q1/02 , C12Q1/44 , C12Q1/48 , C12R1/91 , G01N21/64 , G01N21/78 , G01N33/15 , G01N33/50 , G01N33/542 , G01N33/573 , G01N33/58 , G01N33/52 , G01N33/566
CPC分类号： C12Q1/48 , C12Q1/44 , C12Q1/485 , G01N33/542 , G01N33/5735 , G01N33/582 , G01N2500/02 , Y10T436/143333
摘要： As a cGMP-visualizing probe capable of detecting and quantifying cGMP easily and accurately even in vivo and for a method of detecting and quantifying cGMP by using the same, a cGMP-visualizing probe comprising a polypeptide, which binds specifically to cGMP, and two chromophores having different fluorescence wavelengths each linked respectively to the two terminals of said polypeptide is provided.
摘要翻译：作为能够即使在体内也容易且准确地检测和定量cGMP的cGMP可视化探针，以及通过使用其检测和定量cGMP的方法，可以使用包含特异性结合cGMP的多肽和两种发色团的cGMP可视化探针提供了各自分别连接到所述多肽的两个末端的不同荧光波长。

8. 发明授权

US07425430B2 Probe for visualizing phosphorylation/dephosphorylation of protein and method of detecting and quantifying phosphorylation/dephosphorylation of protein 失效
标题翻译：用于可视化蛋白质的磷酸化/去磷酸化的探针和检测和定量蛋白质的磷酸化/去磷酸化的方法
公开(公告)号：US07425430B2
公开(公告)日：2008-09-16
申请号：US10296313
申请日：2001-03-23
申请人： Yoshio Umezawa , Moritoshi Sato , Takeaki Ozawa
发明人： Yoshio Umezawa , Moritoshi Sato , Takeaki Ozawa
IPC分类号： C07K14/00 , C12Q1/42
CPC分类号： C12Q1/42 , C12Q1/485 , G01N33/542 , G01N2500/02
摘要： As a versatile method of detecting and assaying intracellular protein phosphorylation and dephosphorylation that enables nondestructive monitoring as well as spatial and temporal analysis for living cells, animal bodies, plant bodies and the like, a probe for imaging protein phosphorylation and dephosphorylation, which comprises a tandem fusion unit composed of a substrate domain that contains a phosphorylation and dephosphorylation site, a linker sequence and a phosphorylation recognition domain, interposed between a donor chromophore and an acceptor chromophore that cause fluorescence resonance energy transfer, is used.
摘要翻译：作为检测和测定细胞内蛋白磷酸化和脱磷酸化的多功能方法，其能够对活细胞，动物体，植物体等进行非破坏性监测以及空间和时间分析，用于成像蛋白质磷酸化和脱磷酸化的探针，其包含串联使用由包含磷酸化和去磷酸化位点的底物结构域，接头序列和磷酸化识别结构域组成的融合单元，其插入供体生色团和引起荧光共振能量转移的受体发色团之间。

9. 发明申请

US20070022484A1 Probes for analyzing interaction between proteins and method of analyzing interaction between proteins using the same 审中-公开
标题翻译：用于分析蛋白质之间的相互作用的探针和分析蛋白质之间相互作用的方法
公开(公告)号：US20070022484A1
公开(公告)日：2007-01-25
申请号：US10557992
申请日：2004-05-20
申请人： Yoshio Umezawa , Asami Kaihara , Takeaki Ozawa , Moritoshi Sato
发明人： Yoshio Umezawa , Asami Kaihara , Takeaki Ozawa , Moritoshi Sato
IPC分类号： A01K67/027 , C12Q1/66
CPC分类号： C12Q1/66 , A01K2217/05 , C07K2319/41 , C07K2319/43 , C07K2319/70 , C07K2319/72 , C12N9/0069 , G01N33/542
摘要： The present invention provides a pair of probes for analyzing protein-protein interactions, which comprises a probe A containing at least an N-terminal half polypeptide of split Renilla luciferase, and a probe B containing at least the remaining C-terminal half polypeptide of split Renilla luciferase.
摘要翻译：本发明提供了一对用于分析蛋白质 - 蛋白质相互作用的探针，其包含至少包含分裂的海肾荧光素酶的N-末端半多肽的探针A和至少含有分裂的C末端半多肽的探针B 海肾萤光素酶。

10. 发明申请

US20100273150A1 SINGLE MOLECULE-FORMAT BIOLUMINESCENT PROBE 失效
标题翻译：单分子生物荧光探针
公开(公告)号：US20100273150A1
公开(公告)日：2010-10-28
申请号：US12448881
申请日：2008-01-15
申请人： Yoshio Umezawa , Moritoshi Sato , Hiroaki Tao , SungBae Kim
发明人： Yoshio Umezawa , Moritoshi Sato , Hiroaki Tao , SungBae Kim
IPC分类号： C12Q1/68 , C07H21/04 , C12N15/63
CPC分类号： G01N33/542
摘要： This application aims to provide a single molecule-format probe for detecting a target-specific ligand easily and accurately as an index of the presence or absence of a signal.The invention is a single molecule-format bioluminescent probe for detecting a target-specific ligand in a living cell, which comprises, a ligand-binding molecule of which conformation is changed upon binding to the ligand, and an N-terminal polypeptide (N-LE) and a C-terminal polypeptide (C-LE) of a luminescent enzyme (LE), which are linked to each end of the ligand-binding molecule, respectively, wherein the N-LE and the C-LE self-complement to generate a luminescent signal only upon binding of the ligand to the ligand-binding molecule. The ligand-binding molecule is a fusion molecule carrying a ligand-binding domain (LBD) and an LBD-interacting domain or peptide that interacts with the LBD upon binding of the ligand to the LBD.
摘要翻译：本申请旨在提供用于容易且准确地检测靶特异性配体的单分子格式探针作为信号存在或不存在的指标。本发明是用于检测活细胞中的靶特异性配体的单分子形式的生物发光探针，其包含在与配体结合时构象改变的配体结合分子和N-末端多肽（N- LE）和分别与配体结合分子的每个末端连接的发光酶（LE）的C末端多肽（C-LE），其中N-LE和C-LE自身互补只有当配体结合到配体结合分子时才产生发光信号。配体结合分子是携带配体结合结构域（LBD）的融合分子和与配体结合到LBD时与LBD相互作用的LBD相互作用的结构域或肽。

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