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    • 6. 发明授权
    • Chiral separation of enantiomers by high-speed countercurrent chromatography
    • 通过高速逆流色谱手性分离对映异构体
    • US06337021B1
    • 2002-01-08
    • US08357845
    • 1994-12-16
    • Ying MaYoichiro Ito
    • Ying MaYoichiro Ito
    • B01D1508
    • G01N30/42Y02W10/37
    • Preparative-scale separations of chiral compounds were achieved by high-speed countercurrent chromatography (HSCCC) using a multilayer coil planet centrifuge equipped with a 325 mL capacity column. The separations were performed by two different procedures both utilizing a set of N-(3,5-dintrobenzoyl)-D,L-amino acids as test samples with N-dodecanoyl-L-proline-3,5-dimethylanilide as a chiral selector (Cs). The HSCCC separations were carried out with a two-phase solvent system composed of hexane/ethyl acetate/methanol/water where the chiral selector was added to the organic stationary phase. A second procedure using pH-zone-refining CCC yielded characteristic fused rectangular peaks in which the two isomers were resolved with less than 5% of overlap.
    • 使用装有325mL容量柱的多层线圈行星离心机通过高速逆流色谱(HSCCC)实现手性化合物的制备级分离。 通过两种不同的方法进行分离,两种方法都使用一组N-(3,5-二苯甲酰基)-D,L-氨基酸作为测试样品,其中N-十二酰基-L-脯氨酸-3,5-二甲基苯胺作为手性选择剂 (Cs)。 HSCCC分离用由己烷/乙酸乙酯/甲醇/水组成的两相溶剂系统进行,其中手性选择剂加入到有机固定相中。 使用pH区精炼CCC的第二种方法产生特征融合的矩形峰,其中两种异构体以小于5%重叠的方式解析。