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    • 1. 发明授权
    • Process and analytical agent for the determination of the activity of
glutamate-oxalacetate-transaminase
    • 用于测定谷氨酸 - 乙酸肝素 - 转氨酶活性的方法和分析试剂
    • US4591553A
    • 1986-05-27
    • US500111
    • 1983-06-01
    • Ulfert DenekeWolfgang GruberHans-Georg Batz
    • Ulfert DenekeWolfgang GruberHans-Georg Batz
    • G01N33/52C12Q1/00C12Q1/26C12Q1/48C12Q1/52
    • C12Q1/52Y10S435/805
    • The present invention provides a process for the determination of the activity of glutamateoxalacetate-transaminase (GOT) in aqueous solutions in which(a) .alpha.-ketoglutarate is reacted with alanine-sulphinic acid, catalyzed by GOT, to give pyruvate, sulphur dioxide and glutamate,(b) the resultant pyruvate is reacted with phosphate and oxygen, catalyzed by pyruvate oxidase, to give acetyl phosphate, carbon dioxide and hydrogen peroxide,(c) the hydrogen peroxide and a chromogen is reacted, catalyzed by a peroxidase, to give a dyestuff and water and(d) the amount of dyestuff formed is determined, wherein the chromogen used is N-(3'-sulphonyl-4'-hydroxy-5'-chlorophenyl)-4-aminoantipyrine (SHYC-AA), 4,5-bis-[4-dimethylaminophenyl]-2-[3,5-dimethoxy-4-hydroxyphenyl]-imidazole (B-DDH-imidazole) or a related substance.The present invention also provides an analytical agent for the determination of the activity of GOT, comprising .alpha.-ketoglutarate, alanine-sulphinic acid, a chromogen, peroxidase, pyruvate oxidase, soluble phosphate, buffer and water, wherein the chromogen is N-(3'-sulphonyl-4'-hydroxy-5'-chlorophenyl)-4-aminoantipyrine (SHYC-AA) or 4,5-bis-[4-dimethylaminophenyl]-2-[3,5-dimethoxy-4-hydroxyphenyl]-imidazole (B-DDH-imidazole).
    • 本发明提供了一种测定谷氨酸酰乙酰转氨酶(GOT)在水溶液中的活性的方法,其中(a)α-酮戊二酸与由GOT催化的丙氨酸 - 亚磺酸反应得到丙酮酸,二氧化硫和谷氨酸 ,(b)所得丙酮酸盐与磷酸盐和氧气反应,由丙酮酸氧化酶催化,得到乙酰磷酸盐,二氧化碳和过氧化氢,(c)过氧化氢和色原反应,由过氧化物酶催化,得到 染料和水,(d)确定形成的染料的量,其中使用的色原是N-(3'-磺酰基-4'-羟基-5'-氯苯基)-4-氨基安替比林(SHYC-AA) 5-双 - [4-二甲基氨基苯基] -2- [3,5-二甲氧基-4-羟基苯基] - 咪唑(B-DDH-咪唑)或相关物质。 本发明还提供了用于测定GOT的活性的分析剂,其包括α-酮戊二酸,丙氨酸 - 亚磺酸,色原,过氧化物酶,丙酮酸氧化酶,可溶性磷酸盐,缓冲液和水,其中色原为N-(3 4-磺基-4'-羟基-5'-氯苯基)-4-氨基安替比林(SHYC-AA)或4,5-双 - [4-二甲基氨基苯基] -2- [3,5-二甲氧基-4-羟基苯基] 咪唑(B-DDH-咪唑)。