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    • 1. 发明申请
    • Method of making cell growth surface
    • 细胞生长表面的方法
    • US20060292690A1
    • 2006-12-28
    • US11471576
    • 2006-06-21
    • Tzu-Ying LiuIng-Kae WangSing-Ying HsiehLewis HoYu-Chi WangKing-Ming Chang
    • Tzu-Ying LiuIng-Kae WangSing-Ying HsiehLewis HoYu-Chi WangKing-Ming Chang
    • C12N5/06
    • C12N5/0068C12M25/14C12N2533/70
    • The present invention discloses a three-dimensional porous growth surface made from polysaccharide material, especially the alginic acid, to enhance cell growth surface, promote cell adherence, immobilization and propagation, maintain surface structure integrity, enable programmable degradation, and thus increase cellular production. The present invention teaches several methods: a method to enhance the integrity of the growth surface by protecting the growth surface in a rigid solid support; a method of use for enhancing the performance of the surface; and a method of modifying a growth surface for eukaryotic and/or prokaryotic cells comprising the steps of increasing surface area by creating porous and 3-D structure, treating a surface to encourage cell attachment, promoting cell growth and proliferation, and disposing the growth surface in any conventional cell cultivating device. The growth surface is able to program degradation and release the cell/tissue mass after the culture is completed.
    • 本发明公开了一种由多糖材料特别是藻酸制成的三维多孔生长表面,以增强细胞生长表面,促进细胞粘附,固定和繁殖,保持表面结构完整性,实现可编程降解,从而增加细胞生产。 本发明教导了几种方法:通过保护刚性固体支持物中的生长表面来增强生长表面完整性的方法; 用于增强表面性能的方法; 以及一种修饰真核细胞和/或原核细胞的生长表面的方法,包括以下步骤:通过产生多孔和3-D结构来增加表面积,处理表面以促进细胞附着,促进细胞生长和增殖,以及处置生长表面 在任何常规的细胞培养装置中。 生长表面能够在培养完成后编程降解和释放细胞/组织块。