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1. 发明授权

US5370994A Solid phase assay for urea 失效
标题翻译：尿素固相测定
公开(公告)号：US5370994A
公开(公告)日：1994-12-06
申请号：US165220
申请日：1993-12-10
申请人： Thomas N. Stewart , Glenn P. Vonk , James P. Mapes
发明人： Thomas N. Stewart , Glenn P. Vonk , James P. Mapes
IPC分类号： C12Q1/58 , G01N33/535 , G01N33/543 , G01N33/58 , G01N21/00
CPC分类号： C12Q1/58 , G01N33/54366 , G01N33/54386 , G01N33/581 , Y10S435/805 , Y10S435/97 , Y10S436/81 , Y10S436/904
摘要： A method for detecting urea in a liquid sample in which urease is adsorbed on a solid support such as a membrane and contacted with a solution suspected of containing urea, a pH-dependent reducing agent and a tetrazolium salt. When urea is present in the solution, the adsorbed urease converts it to ammonia, thus raising the pH and causing the pH-dependent reducing agent to reduce the tetrazolium salt to an insoluble colored formazan. The formazan precipitates as a detectable spot on the solid support, indicating the presence of urea in the liquid sample.
摘要翻译：用于检测液体样品中尿素的方法，其中尿素酶吸附在固体支持物如膜上并与怀疑含有尿素的溶液，pH依赖性还原剂和四唑鎓盐接触。当溶液中存在尿素时，吸附的脲酶将其转化为氨，从而提高pH并引起pH依赖性还原剂将四唑盐还原成不溶性的有色甲。。甲an作为固体支持物上的可检测点沉淀，表明液体样品中存在尿素。

2. 发明授权

US5139934A Substrate composition and method for solid phase urease immunoassay 失效
标题翻译：基质组合物和固相免疫免疫法的方法
公开(公告)号：US5139934A
公开(公告)日：1992-08-18
申请号：US528526
申请日：1990-05-25
申请人： Thomas N. Stewart , Glenn P. Vonk , James P. Mapes
发明人： Thomas N. Stewart , Glenn P. Vonk , James P. Mapes
IPC分类号： C12Q1/58 , G01N33/535 , G01N33/543 , G01N33/58
CPC分类号： C12Q1/58 , G01N33/54366 , G01N33/54386 , G01N33/581 , Y10S435/805 , Y10S435/97 , Y10S436/81 , Y10S436/904
摘要： A method for enzyme immunoassay of a ligand includes urease as a label. A bound fraction of ligand and antiligand conjugated to urease is formed on a solid support. The urease component of the bound fraction is contacted with a substrate composition for urease which includes a compound converted to ammonia by the urease, a tetrazolium salt and a pH dependent reducing agent which reduces the tetrazolium salt when the pH of the assay medium has been raised by the ammonia. The tetrazolium salt is reduced to a colored insoluble formazan which precipitates as a detectable spot on the support. The invention includes the substrate composition and a kit of materials for performing the assay.

3. 发明授权

US5328831A Substrate composition for solid phase urease immunoassay 失效
标题翻译：固相脲酶免疫测定的底物组成
公开(公告)号：US5328831A
公开(公告)日：1994-07-12
申请号：US851602
申请日：1992-03-16
申请人： Thomas N. Stewart , Glenn P. Vonk , James P. Mapes
发明人： Thomas N. Stewart , Glenn P. Vonk , James P. Mapes
IPC分类号： C12Q1/58 , G01N33/535 , G01N33/543 , G01N33/58 , B12Q1/58 , C12N9/96 , C12Q1/00
CPC分类号： C12Q1/58 , G01N33/54366 , G01N33/54386 , G01N33/581 , Y10S435/805 , Y10S435/97 , Y10S436/81 , Y10S436/904
摘要： A substrate composition for use in a solid phase enzyme assay for urease or in a solid phase enzyme immunoassay which includes urease as a label. The substrate composition includes a compound converted by urease to ammonia and a pH dependent reducing agent which reduces a tetrazolium salt when the pH of the medium has been raised by the ammonia produced. The tetrazolium salt may optionally be included in the substrate composition. Reduction of the tetrazolium salt produces a colored insoluble formazan which precipitates on the solid phase as an indication of the presence of urease.
摘要翻译：用于脲酶或固相酶免疫测定的固相酶测定的底物组合物，其包括脲酶作为标记。底物组合物包括通过脲酶转化为氨的化合物和pH依赖性还原剂，当培养基的pH被所产生的氨升高时，还原四氮唑盐。可以任选地将四唑盐包括在底物组合物中。四唑盐的还原产生着色的不溶性甲an，其在固相上析出，作为尿素酶存在的指示。

4. 发明申请

US20110177959A1 Methods and Devices for Detecting Kidney Transplant Rejection 审中-公开
标题翻译：检测肾移植排斥的方法和装置
公开(公告)号：US20110177959A1
公开(公告)日：2011-07-21
申请号：US12852322
申请日：2010-08-06
申请人： Michael D. Spain , James P. Mapes , Samuel T. Labrie , Ralph L. McDade , Dominic P. Eisinger , Karri L. Ballard , Daniel R. Salomon , Michael Abecassis
发明人： Michael D. Spain , James P. Mapes , Samuel T. Labrie , Ralph L. McDade , Dominic P. Eisinger , Karri L. Ballard , Daniel R. Salomon , Michael Abecassis
IPC分类号： C40B30/02
CPC分类号： G01N33/6893 , G01N33/5302 , G01N33/566 , G01N2333/47 , G01N2333/4703 , G01N2333/4706 , G01N2333/4725 , G01N2333/4727 , G01N2333/475 , G01N2333/52 , G01N2333/70503 , G01N2333/70539 , G01N2333/765 , G01N2333/775 , G01N2333/8139 , G01N2333/8146 , G01N2333/82 , G01N2333/91177 , G01N2800/34 , G01N2800/347 , G01N2800/52 , G01N2800/56 , G01N2800/60 , Y10T436/147777
摘要： Methods and devices for diagnosing, monitoring, or determining kidney transplant rejection or an associated disorder in a mammal are described. In particular, methods and devices for diagnosing, monitoring, or determining kidney transplant rejection or an associated disorder using measured concentrations of a combination of three or more analytes in a test sample taken from the mammal are described.
摘要翻译：描述了用于诊断，监测或确定哺乳动物的肾移植排斥或相关病症的方法和装置。具体地，描述了使用从哺乳动物获取的测试样品中测量浓度的三种或更多种分析物的组合来诊断，监测或确定肾移植排斥或相关疾病的方法和装置。

5. 发明申请

US20110065137A1 Methods and Devices for Detecting Obstructive Uropathy and Associated Disorders 有权
标题翻译：用于检测阻塞性尿路疾病和相关疾病的方法和装置
公开(公告)号：US20110065137A1
公开(公告)日：2011-03-17
申请号：US12852236
申请日：2010-08-06
申请人： Samuel T. LaBrie , James P. Mapes , Ralph L. McDade , Dominic Eisinger , Karri L. Ballard , Michael D. Spain
发明人： Samuel T. LaBrie , James P. Mapes , Ralph L. McDade , Dominic Eisinger , Karri L. Ballard , Michael D. Spain
IPC分类号： C12Q1/48 , G01N33/70 , G01N33/68
CPC分类号： G01N33/6893 , G01N33/5302 , G01N33/566 , G01N2333/47 , G01N2333/4703 , G01N2333/4706 , G01N2333/4725 , G01N2333/4727 , G01N2333/475 , G01N2333/52 , G01N2333/70503 , G01N2333/70539 , G01N2333/765 , G01N2333/775 , G01N2333/8139 , G01N2333/8146 , G01N2333/82 , G01N2333/91177 , G01N2800/34 , G01N2800/347 , G01N2800/52 , G01N2800/56 , G01N2800/60 , Y10T436/147777
摘要： Methods and devices for diagnosing, monitoring, or determining obstructive uropathy or an associated disorder in a mammal are described. In particular, methods and devices for diagnosing, monitoring, or determining obstructive uropathy or an associated disorder using measured concentrations of a combination of three or more analytes in a test sample taken from the mammal are described.
摘要翻译：描述了用于诊断，监测或确定哺乳动物的阻塞性尿病或相关疾病的方法和装置。具体地，描述了使用从哺乳动物取得的测试样品中测量浓度的三种或更多种分析物的组合来诊断，监测或确定阻塞性尿病或相关疾病的方法和装置。

6. 发明授权

US5874216A Indirect label assay device for detecting small molecules and method of use thereof 失效
公开(公告)号：US5874216A
公开(公告)日：1999-02-23
申请号：US606385
申请日：1996-02-23
申请人： James P. Mapes
发明人： James P. Mapes
IPC分类号： G01N33/543 , G01N33/558 , G01N33/533
CPC分类号： G01N33/54366 , G01N33/558 , Y10S435/962 , Y10S435/969 , Y10S435/97 , Y10S436/823 , Y10S436/825
摘要： A chromatographic strip positive readout binding assay device and method suitable for quick, sensitive and reliable field testing for small molecules such as environmental contaminants, drugs of abuse, therapeutic drugs and hormones. The detectable label is not attached to either the analyte or to the analyte receptor. Low affinity binding pairs can be used in the positive readout binding assay.

7. 发明授权

US5155022A Assay for lyme disease 失效
标题翻译：测定LYME疾病
公开(公告)号：US5155022A
公开(公告)日：1992-10-13
申请号：US652681
申请日：1991-02-08
申请人： Ali Naqui , Liane F. Gossett , James P. Mapes
发明人： Ali Naqui , Liane F. Gossett , James P. Mapes
IPC分类号： G01N33/569 , G01N33/58
CPC分类号： G01N33/586 , G01N33/56911 , Y10S435/962 , Y10S435/975 , Y10S436/80 , Y10S436/808 , Y10S436/81 , Y10S436/811 , Y10S436/825 , Y10S436/829 , Y10T436/25 , Y10T436/25125
摘要： Diagnosis of Lyme disease by immunoassay for anti-Borrelia burgdorferi antibodies in the serum of a patient includes adsorption of crossreactive antibodies in the serum with antigen of Acinetobacter calcoaceticus or Treponema phagedensis prior to binding of anti-Borrelia antibodies to Borrelia burgdorferi antigen absorbed onto a solid support. The preferred assay is a flow-through assay using a porous membrane as the support and a dye-loaded liposome conjugated to a goat antihuman antibody for detection. The invention includes a kit of materials for performing the assay.

8. 发明申请

US20110065598A1 Methods and Devices for Detecting Diabetic Nephropathy and Associated Disorders 审中-公开
标题翻译：检测糖尿病肾病和相关疾病的方法和装置
公开(公告)号：US20110065598A1
公开(公告)日：2011-03-17
申请号：US12852282
申请日：2010-08-06
申请人： Samuel T. Labrie , James P. Mapes , Ralph L. McDade , Dominic Eisinger , Karri L. Ballard
发明人： Samuel T. Labrie , James P. Mapes , Ralph L. McDade , Dominic Eisinger , Karri L. Ballard
IPC分类号： C40B30/04
CPC分类号： G01N33/6893 , G01N33/5302 , G01N33/566 , G01N2333/47 , G01N2333/4703 , G01N2333/4706 , G01N2333/4725 , G01N2333/4727 , G01N2333/475 , G01N2333/52 , G01N2333/70503 , G01N2333/70539 , G01N2333/765 , G01N2333/775 , G01N2333/8139 , G01N2333/8146 , G01N2333/82 , G01N2333/91177 , G01N2800/34 , G01N2800/347 , G01N2800/52 , G01N2800/56 , G01N2800/60 , Y10T436/147777
摘要： Methods and devices for diagnosing, monitoring, or determining diabetic nephropathy or an associated disorder in a mammal are described. In particular, methods and devices for diagnosing, monitoring, or determining diabetic nephropathy or an associated disorder using measured concentrations of a combination of three or more analytes in a test sample taken from the mammal are described.
摘要翻译：描述了用于诊断，监测或确定哺乳动物中的糖尿病性肾病或相关疾病的方法和装置。具体地，描述了使用从哺乳动物获取的测试样品中测量的三种或更多种分析物的组合的测量浓度来诊断，监测或确定糖尿病性肾病或相关疾病的方法和装置。

9. 发明申请

US20090068680A1 Method for Characterizing Immune Disorders 审中-公开
标题翻译：表征免疫功能障碍的方法
公开(公告)号：US20090068680A1
公开(公告)日：2009-03-12
申请号：US12273292
申请日：2008-11-18
申请人： James P. Mapes , James W. Jacobson , Harold N. Baker , Michael D. Spain
发明人： James P. Mapes , James W. Jacobson , Harold N. Baker , Michael D. Spain
IPC分类号： G01N33/53
CPC分类号： G01N33/54313 , G01N33/564 , G01N2800/24 , Y10S435/973 , Y10S436/811
摘要： Methods for characterizing immune diseases are provided which include exposing a sample of biological fluid from a patient to a pooled population of particles, wherein a first subset of particles is bound to a reactant that binds an antibody and a second subset of particles is bound to a reactant that binds an antigen. The methods further include determining a ratio of measured amounts of the antibody and the antigen in the sample. In some cases, the ratio is compared to one or more standard ratios representing differing states of the disease to determine a state of the disease within the patient. In other embodiments, the steps of exposing and determining a ratio of antibody to antigen are repeated for one or more additional samples of biological fluid taken from the patient. In such cases, at least two ratios are compared to analyze a progression of the disease.
摘要翻译：提供了表征免疫疾病的方法，其包括将患者的生物流体样品暴露于汇集的颗粒群体，其中颗粒的第一子集与结合抗体的反应物结合，并且第二子粒子与结合抗原的反应物。所述方法还包括确定样品中抗体和抗原的测量量的比例。在一些情况下，将该比率与表示疾病状态不同的一个或多个标准比例进行比较，以确定患者内疾病的状态。在其它实施方案中，对从患者取得的生物流体的一个或多个另外的样本重复暴露和确定抗体与抗原的比例的步骤。在这种情况下，比较至少两个比率来分析疾病的进展。

10. 发明授权

US07189516B2 Method for characterizing autoimmune disorders 有权
标题翻译：表征自身免疫性疾病的方法
公开(公告)号：US07189516B2
公开(公告)日：2007-03-13
申请号：US10222867
申请日：2002-08-19
申请人： James P. Mapes , James W. Jacobson , Harold N. Baker , Michael D. Spain
发明人： James P. Mapes , James W. Jacobson , Harold N. Baker , Michael D. Spain
IPC分类号： G01N33/53
CPC分类号： G01N33/54313 , G01N33/564 , G01N2800/24 , Y10S435/973 , Y10S436/811
摘要： The invention relates to methods of characterizing autoimmune diseases by detecting and measuring at least one analyte using multiplexed assay systems. According to one embodiment, a target is detected and measured by different bead sets having different reactants. According to another approach the ratio of self-antigen to autoantibody is measured by exposing a sample suspected of containing self-antigen and autoantibody to a bead set associated with monoclonal antibody specific for the self-antigen and a bead set associated with the self-antigen.
摘要翻译：本发明涉及通过使用多重测定系统检测和测量至少一种分析物来表征自身免疫性疾病的方法。根据一个实施例，通过具有不同反应物的不同珠组检测和测量靶。根据另一种方法，通过将怀疑含有自身抗原和自身抗体的样品暴露于与自身抗原特异性的单克隆抗体相关联的珠粒组和与自身抗原相关的珠粒组来测量自身抗原与自身抗体的比例。

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