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1. 发明授权

US6077669A Kit and method for fluorescence based detection assay 失效
标题翻译：用于基于荧光的检测试剂盒和方法
公开(公告)号：US6077669A
公开(公告)日：2000-06-20
申请号：US964020
申请日：1997-11-04
申请人： Michael C. Little , Glenn P. Vonk
发明人： Michael C. Little , Glenn P. Vonk
IPC分类号： G01N33/50 , C12M1/00 , C12N15/09 , C12Q1/68 , G01N21/78 , G01N33/53 , G01N33/566 , C12P19/34
CPC分类号： C12Q1/6825 , Y10S435/968
摘要： The present invention relates to kits and methods for conducting fluorescence based detection assays. The kits are configured in a manner to perform the method so as to reduce or eliminate interfering fluorescence signal.
摘要翻译：本发明涉及用于进行基于荧光的检测测定的试剂盒和方法。这些试剂盒被配置为执行该方法以减少或消除干扰荧光信号。

2. 发明授权

US6054279A Detection of nucleic acids by fluorescence quenching 失效
标题翻译：通过荧光猝灭检测核酸
公开(公告)号：US6054279A
公开(公告)日：2000-04-25
申请号：US120916
申请日：1998-07-20
申请人： James G. Nadeau , J. Bruce Pitner , James L. Schram , C. Preston Linn , Glenn P. Vonk , G. Terrance Walker
发明人： James G. Nadeau , J. Bruce Pitner , James L. Schram , C. Preston Linn , Glenn P. Vonk , G. Terrance Walker
IPC分类号： G01N21/64 , C07H21/00 , C12N15/09 , C12Q1/68 , G01N33/58 , C21Q1/68
CPC分类号： C12Q1/6818 , C12Q1/6825
摘要： Single-stranded signal primers are modified by linkage to two dyes which form a donor/acceptor dye pair. The two dyes are positioned in sufficiently close spatial proximity on the signal primer that the fluorescence of the first dye is quenched by the second dye. The signal primer may further comprise a restriction endonuclease recognition site (RERS) between the two dyes. As the signal primer is initially single-stranded and remains single-stranded in the absence of target, the restriction endonuclease recognition site is not cleavable or nickable by the restriction endonuclease. In the presence of target, however, signal primer and the restriction endonuclease recognition site are rendered double-stranded and cleavable or nickable by the restriction endonuclease. Cleavage or nicking separates the two dyes and a change in fluorescence due to decreased quenching is detected as an indication of the presence of the target sequence or of target sequence amplification.
摘要翻译：通过连接到形成供体/受体染料对的两种染料来修饰单链信号引物。这两种染料位于信号引物足够接近的空间上，第一种染料的荧光被第二种染料淬灭。信号引物还可以包含两种染料之间的限制性内切核酸酶识别位点（RERS）。由于信号引物最初是单链的，并且在不存在靶标的情况下保持单链，限制性内切核酸酶识别位点不受限制性内切核酸酶的切割或切割。然而，在靶的存在下，信号引物和限制性内切核酸酶识别位点被限制性内切核酸酶双链和切割或切割。切割或切口分离两种染料，并且由于猝灭降低引起的荧光变化被检测为目标序列或靶序列扩增的存在的指示。

3. 发明授权

US5691145A Detection of nucleic acids using G-quartets 失效
标题翻译：使用G四重奏检测核酸
公开(公告)号：US5691145A
公开(公告)日：1997-11-25
申请号：US703755
申请日：1996-08-27
申请人： J. Bruce Pitner , Glenn P. Vonk , James G. Nadeau
发明人： J. Bruce Pitner , Glenn P. Vonk , James G. Nadeau
IPC分类号： G01N33/58 , C07H21/04 , C12N15/09 , C12Q1/68 , G01N21/78 , C07H21/02 , C12P19/34
CPC分类号： C12Q1/6818 , Y10S436/80 , Y10S436/805 , Y10T436/143333
摘要： Oligonucleotides which form G-quartet structures have been found to be useful in fluorescence assays to detect a selected nucleic acid sequence. When one end of the oligonucleotide is labeled with a donor fluorophore and the other end is labeled with an acceptor dye, the folding of the molecule in the G-quartet structure brings the donor-acceptor pair into close proximity, allowing an interaction between the two labels which results in quenching of donor fluorescence or a change in other fluorescence properties which are the result of the interaction of two dyes in close proximity. The G-quartet structure unfolds upon hybridization to its complementary sequence, increasing the distance between the two dye labels. This results in decreased donor quenching or a change in another proximity-related fluorescence parameter. The associated increase in donor fluorescence intensity or the change in another fluorescence parameter may be monitored as an indication of the presence of a selected nucleic acid sequence. Alternatively, in some cases a decrease in acceptor fluorescence may be monitored as an indication of the presence of the selected nucleic acid sequence when the acceptor is also a fluorophore.
摘要翻译：已经发现形成G-四重结构的寡核苷酸可用于荧光测定以检测所选择的核酸序列。当寡核苷酸的一端用供体荧光团标记，另一端用受体染料标记时，分子在G四重结构中的折叠使供体 - 受体对紧密接近，允许两者之间的相互作用导致供体荧光猝灭的标记或其他荧光性质的变化，这两种染料是两种染料相互作用的结果。 G-四重结构在与其互补序列杂交时展开，增加两个染料标记之间的距离。这导致供体猝灭减少或另一邻近相关荧光参数的变化。可以监测供体荧光强度的相关增加或另一荧光参数的变化作为选择的核酸序列的存在的指示。或者，在一些情况下，当受体也是荧光团时，可以监测受体荧光的降低作为选择的核酸序列的存在的指示。

4. 发明授权

US5370994A Solid phase assay for urea 失效
标题翻译：尿素固相测定
公开(公告)号：US5370994A
公开(公告)日：1994-12-06
申请号：US165220
申请日：1993-12-10
申请人： Thomas N. Stewart , Glenn P. Vonk , James P. Mapes
发明人： Thomas N. Stewart , Glenn P. Vonk , James P. Mapes
IPC分类号： C12Q1/58 , G01N33/535 , G01N33/543 , G01N33/58 , G01N21/00
CPC分类号： C12Q1/58 , G01N33/54366 , G01N33/54386 , G01N33/581 , Y10S435/805 , Y10S435/97 , Y10S436/81 , Y10S436/904
摘要： A method for detecting urea in a liquid sample in which urease is adsorbed on a solid support such as a membrane and contacted with a solution suspected of containing urea, a pH-dependent reducing agent and a tetrazolium salt. When urea is present in the solution, the adsorbed urease converts it to ammonia, thus raising the pH and causing the pH-dependent reducing agent to reduce the tetrazolium salt to an insoluble colored formazan. The formazan precipitates as a detectable spot on the solid support, indicating the presence of urea in the liquid sample.
摘要翻译：用于检测液体样品中尿素的方法，其中尿素酶吸附在固体支持物如膜上并与怀疑含有尿素的溶液，pH依赖性还原剂和四唑鎓盐接触。当溶液中存在尿素时，吸附的脲酶将其转化为氨，从而提高pH并引起pH依赖性还原剂将四唑盐还原成不溶性的有色甲。。甲an作为固体支持物上的可检测点沉淀，表明液体样品中存在尿素。

5. 发明授权

US4940669A Sac including a detectable metal marker and use thereof in an assay 失效
标题翻译： Sac包括可检测的金属标记物及其在测定中的用途
公开(公告)号：US4940669A
公开(公告)日：1990-07-10
申请号：US49971
申请日：1987-05-15
申请人： Daniel B. Wagner , Glenn P. Vonk
发明人： Daniel B. Wagner , Glenn P. Vonk
IPC分类号： G01N33/58
CPC分类号： G01N33/586 , Y10S436/80 , Y10S436/821 , Y10S436/829
摘要： A sac, in particular a vesicle, having a detectable metal, in particular a rear earth metal, encapsulated therein. The sac may be sensitized with a ligand and used in an assay. The vesicle is stored and/or used in an aqueous solution which includes the rare earth metal to increase the concentration of metal in the sac.
摘要翻译：具有可检测金属的囊，特别是囊泡，其囊封在其中。囊可以用配体致敏并用于测定。将囊泡储存和/或用于包括稀土金属的水溶液中以增加囊中金属的浓度。

6. 发明授权

US5314801A Probes to Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium paratuberculosis 失效
标题翻译：对鸟分枝杆菌，胞内分枝杆菌和副结核分枝杆菌的探针
公开(公告)号：US5314801A
公开(公告)日：1994-05-24
申请号：US973342
申请日：1992-11-06
申请人： Colleen M. Nycz , James L. Schram , Daryl D. Shank , Glenn P. Vonk
发明人： Colleen M. Nycz , James L. Schram , Daryl D. Shank , Glenn P. Vonk
IPC分类号： C12Q1/68 , C07H21/00 , C12P19/34
CPC分类号： C12Q1/689
摘要： The invention provides methods and nucleic acid probes for deleting, amplifying, and isolating sequences of Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium paratuberculosis.
摘要翻译：本发明提供了用于缺失，扩增和分离鸟分枝杆菌，胞内分枝杆菌和副结核分枝杆菌序列的方法和核酸探针。

7. 发明授权

US4978625A Fluorescence immunoassay using water insoluble dyes 失效
标题翻译：荧光免疫测定使用水不溶性染料
公开(公告)号：US4978625A
公开(公告)日：1990-12-18
申请号：US109689
申请日：1987-10-19
申请人： Daniel B. Wagner , Glenn P. Vonk , Thomas J. Mercolino
发明人： Daniel B. Wagner , Glenn P. Vonk , Thomas J. Mercolino
IPC分类号： G01N33/58
CPC分类号： G01N33/586 , Y10S436/80 , Y10S436/808 , Y10S436/829
摘要： In a method for fluorescence immunoassay of an analyte, an antianalyte attached to the surface of a solid support is contacted with the analyte and a tracer which includes a substantially water insoluble fluorescent dye occluded in the nonaqueous portion of a sac. After binding reactions involving the antianalyte, analyte and tracer, the solid support is separated, excitation light is applied and fluorescence from dye in intact sacs bound to the solid support is measured and compared to fluorescence measured when known quantities of analyte are assayed. The invention includes a kit of materials useful in performing an immunoassay in accordance with the method of the invention.
摘要翻译：在用于分析物的荧光免疫测定的方法中，附着在固体支持物表面的抗分析物与分析物和示踪剂接触，所述示踪剂包括封闭在囊的非水部分中的基本上不溶于水的荧光染料。在涉及抗分析物，分析物和示踪剂的结合反应之后，分离固体支持物，施加激发光，并测量与固体支持物结合的完整囊中来自染料的荧光，并与测定已知量的分析物时测量的荧光进行比较。本发明包括用于根据本发明的方法进行免疫测定的材料试剂盒。

8. 发明授权

US5919630A Detection of nucleic acids by fluorescence quenching 失效
公开(公告)号：US5919630A
公开(公告)日：1999-07-06
申请号：US186030
申请日：1998-11-04
申请人： James G. Nadeau , J. Bruce Pitner , James L. Schram , C. Preston Linn , Glenn P. Vonk , G. Terrance Walker
发明人： James G. Nadeau , J. Bruce Pitner , James L. Schram , C. Preston Linn , Glenn P. Vonk , G. Terrance Walker
IPC分类号： G01N21/64 , C07H21/00 , C12N15/09 , C12Q1/68 , G01N33/58 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/6818 , C12Q1/6825
摘要： Single-stranded signal primers are modified by linkage to two dyes which form a donor/acceptor dye pair. The two dyes are positioned in sufficiently close spatial proximity on the signal primer that the fluorescence of the first dye is quenched by the second dye. The signal primer may further comprise a restriction endonuclease recognition site (RERS) between the two dyes. As the signal primer is initially single-stranded and remains single-stranded in the absence of target, the restriction endonuclease recognition site is not cleavable or nickable by the restriction endonuclease. In the presence of target, however, signal primer and the restriction endonuclease recognition site are rendered double-stranded and cleavable or nickable by the restriction endonuclease. Cleavage or nicking separates the two dyes and a change in fluorescence due to decreased quenching is detected as an indication of the presence of the target sequence or of target sequence amplification.

9. 发明授权

US5641629A Spectroscopically detectable nucleic acid ligands 失效
标题翻译：光谱可检测的核酸配体
公开(公告)号：US5641629A
公开(公告)日：1997-06-24
申请号：US376329
申请日：1995-01-20
申请人： James B. Pitner , Douglas P. Malinowski , Glenn P. Vonk , Larry Gold
发明人： James B. Pitner , Douglas P. Malinowski , Glenn P. Vonk , Larry Gold
IPC分类号： G01N33/566 , C07B61/00 , C07H19/06 , C07H19/10 , C07H21/00 , C07K14/00 , C12N9/12 , C12N15/09 , C12N15/10 , C12N15/115 , C12Q1/37 , C12Q1/68 , C12Q1/70 , F02B75/02 , G01N33/531 , G01N33/532 , G01N33/535 , G01N33/569 , G01N33/68 , G01N33/76 , C07H21/02 , C07H21/04
CPC分类号： C07H21/00 , A61K47/48076 , A61K47/48092 , B82Y5/00 , C07H19/06 , C07H19/10 , C07K14/001 , C12N15/1048 , C12N15/115 , C12N9/1276 , C12Q1/37 , C12Q1/6811 , C12Q1/6816 , G01N33/531 , G01N33/532 , G01N33/535 , G01N33/56988 , G01N33/68 , G01N33/76 , C12N2310/13 , C12N2310/322 , C12N2310/53 , C40B40/00 , F02B2075/027 , G01N2333/163 , G01N2333/575 , G01N2333/62 , G01N2333/8125 , G01N2333/96433 , G01N2333/96436 , G01N2333/96455 , G01N2333/96486 , G01N2333/966 , G01N2333/9726 , G01N2333/974 , G01N2333/976
摘要： The present invention relates to methods of using spectroscopically detectable labeled receptor molecules to determine the presence or absence of a target compound in a sample. In one embodiment, spectroscopically detectable labeled nucleic acid ligands are used to determine the presence or absence of biological targets of interest in biological samples.
摘要翻译：本发明涉及使用光谱可检测标记的受体分子来确定样品中目标化合物的存在或不存在的方法。在一个实施方案中，使用光谱可检测的标记的核酸配体来确定生物样品中感兴趣的生物靶标的存在或不存在。

10. 发明授权

US5328831A Substrate composition for solid phase urease immunoassay 失效
标题翻译：固相脲酶免疫测定的底物组成
公开(公告)号：US5328831A
公开(公告)日：1994-07-12
申请号：US851602
申请日：1992-03-16
申请人： Thomas N. Stewart , Glenn P. Vonk , James P. Mapes
发明人： Thomas N. Stewart , Glenn P. Vonk , James P. Mapes
IPC分类号： C12Q1/58 , G01N33/535 , G01N33/543 , G01N33/58 , B12Q1/58 , C12N9/96 , C12Q1/00
CPC分类号： C12Q1/58 , G01N33/54366 , G01N33/54386 , G01N33/581 , Y10S435/805 , Y10S435/97 , Y10S436/81 , Y10S436/904
摘要： A substrate composition for use in a solid phase enzyme assay for urease or in a solid phase enzyme immunoassay which includes urease as a label. The substrate composition includes a compound converted by urease to ammonia and a pH dependent reducing agent which reduces a tetrazolium salt when the pH of the medium has been raised by the ammonia produced. The tetrazolium salt may optionally be included in the substrate composition. Reduction of the tetrazolium salt produces a colored insoluble formazan which precipitates on the solid phase as an indication of the presence of urease.
摘要翻译：用于脲酶或固相酶免疫测定的固相酶测定的底物组合物，其包括脲酶作为标记。底物组合物包括通过脲酶转化为氨的化合物和pH依赖性还原剂，当培养基的pH被所产生的氨升高时，还原四氮唑盐。可以任选地将四唑盐包括在底物组合物中。四唑盐的还原产生着色的不溶性甲an，其在固相上析出，作为尿素酶存在的指示。

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