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1. 发明授权

US6140077A Method for producing phytase 失效
标题翻译：植酸酶的生产方法
公开(公告)号：US6140077A
公开(公告)日：2000-10-31
申请号：US14583
申请日：1998-01-28
申请人： Takeshi Nakamura , Tadashi Suzuki , Junko Tokuda , Nobuo Kato , Yasuyoshi Sakai , Daisuke Mochizuki , Hitoshi Takahashi
发明人： Takeshi Nakamura , Tadashi Suzuki , Junko Tokuda , Nobuo Kato , Yasuyoshi Sakai , Daisuke Mochizuki , Hitoshi Takahashi
IPC分类号： C12N9/16 , C12N15/55 , C12N15/81 , C12P21/02 , C12P21/06 , C12N1/14 , C12N15/00
CPC分类号： C12P21/02 , C12N15/815 , C12N9/16
摘要： A method for producing phytase using a Candida boidinii transformant, by which phytase is secreted to liquid medium without substantially increasing the number of the transformant cells is disclosed. In this method, cells of a Candida boidinii transformant which is transformed with a recombinant vector comprising a promoter inducible with methanol and a gene located downstream of said promoter and operably linked to said promoter, which gene encodes a polypeptide having phytase activity, are cultured in a liquid culture medium containing phosphoric acid or a phosphate compound in an amount effective of restricting proliferation of said cells of Candida boidinii transformant such that the amount of said cells of Candida boidinii transformant is not substantially increased, while adding methanol to the culture medium, so as to make said cells secrete said phytase.
摘要翻译：公开了一种使用布氏念珠菌转化体生产植酸酶的方法，其中植酸酶分泌到液体培养基中而不显着增加转化体细胞的数量。在该方法中，使用包含可诱导甲醇的启动子和位于所述启动子下游的基因并与所述启动子连接的重组载体转化的念珠菌转化体的细胞，所述基因编码具有植酸酶活性的多肽，其基因编码具有植酸酶活性的多肽含有磷酸或磷酸盐化合物的液体培养基，其量有效地限制博伊迪尼氏假丝酵母转化体的所述细胞的增殖，使得在培养基中加入甲醇，使得所述Boidinii念珠菌转化体的细胞数量基本上不增加，因此以使所述细胞分泌所述植酸酶。

2. 发明授权

US6033867A Fructosyl amino acid oxidase, process for producing the same, and method of assaying amadori compounds using the enzyme 失效
标题翻译：果糖基氨基酸氧化酶，其制备方法以及使用该酶测定阿马多利化合物的方法
公开(公告)号：US6033867A
公开(公告)日：2000-03-07
申请号：US77523
申请日：1998-07-29
申请人： Nobuo Kato , Yasuyoshi Sakai , Yoshiki Tani , Hiroshi Fukuya , Masayuki Yagi , Toshikatsu Sakai , Kaori Ishimaru , Satoshi Cyousa
发明人： Nobuo Kato , Yasuyoshi Sakai , Yoshiki Tani , Hiroshi Fukuya , Masayuki Yagi , Toshikatsu Sakai , Kaori Ishimaru , Satoshi Cyousa
IPC分类号： C12N9/06 , C12Q1/26 , C12N1/14 , C12N9/04 , C12N9/62
CPC分类号： C12N9/0022 , C12Q1/26 , G01N2333/906 , G01N2333/90633
摘要： The present invention relates to a fructosyl amino acid oxidase that is obtainable from a culture of a strain of the genus Aspergillus. This strain grows in a selective medium containing at least either of fructosyl lysine and fructosyl-N.sup..alpha. -Z-lysine and is capable of producing a fructosyl amino acid oxidase.
摘要翻译： PCT No.PCT / JP96 / 03515 Sec。 371日期：1998年5月29日 102（e）日期1989年5月29日PCT 1996年12月2日PCT公布。公开号WO97 / 20039 日期：1997年6月5日本发明涉及可从曲霉属菌株的培养物获得的果糖基氨基酸氧化酶。该菌株在含有果糖基赖氨酸和果糖基-Nα-Z-赖氨酸中的至少一种的选择性培养基中生长，并且能够产生果糖基氨基酸氧化酶。

3. 发明授权

US5948659A Recombinant fructosyl amino acid oxidase 失效
标题翻译：重组果糖基氨基酸氧化酶
公开(公告)号：US5948659A
公开(公告)日：1999-09-07
申请号：US031059
申请日：1998-02-26
申请人： Nobuo Kato , Yasuyoshi Sakai , Yoshiki Tani , Hiroshi Fukuya
发明人： Nobuo Kato , Yasuyoshi Sakai , Yoshiki Tani , Hiroshi Fukuya
IPC分类号： C12N15/09 , C07H21/04 , C12N1/21 , C12N9/06 , C12N15/53 , C12P21/02 , C12Q1/26 , C12R1/19 , C12N9/02
CPC分类号： C12N9/0022
摘要： The present invention provides a recombinant protein which shows fructosyl amino acid oxidase activity, a DNA encoding the same, an expression vector containing the DNA, a transformant transformed by the expression vector, and the method of preparing recombinant fructosyl amino acid oxidase by culturing the resultant transformant, and the recombinant fructosyl amino acid oxidase thus obtained.
摘要翻译：本发明提供了显示果糖基氨基酸氧化酶活性的重组蛋白质，编码该DNA的重组蛋白质，含有该DNA的表达载体，通过表达载体转化的转化体，以及通过培养所得物制备重组果糖基氨基酸氧化酶的方法转化体和由此获得的重组果糖基氨基酸氧化酶。

4. 发明授权

US5789221A Fructosyl amino acid oxidase and process for producing the same 失效
公开(公告)号：US5789221A
公开(公告)日：1998-08-04
申请号：US396761
申请日：1995-03-01
申请人： Nobuo Kato , Yasuyoshi Sakai , Yoshiki Tani , Masayuki Yagi , Fumiyo Funatsu
发明人： Nobuo Kato , Yasuyoshi Sakai , Yoshiki Tani , Masayuki Yagi , Fumiyo Funatsu
IPC分类号： C12N1/26 , C12N9/06 , C12Q1/26 , C12N9/04 , C12N9/02 , C12N9/08
CPC分类号： C12N9/0022 , C12N1/26 , C12Q1/26 , C12R1/77 , Y10S435/929
摘要： A novel fructosyl amino acid oxidase derived from genus Fusarium or Gibberella, a process for producing the enzyme, an assay of an amadori compound using the enzyme, a reagent or a kit containing the enzyme, a process for producing fructosyl lysine and/or fructosyl N.sup..alpha. -Z-lysine as a substrate of a fructosyl amino acid oxidase, which is useful for screening and/or culturing a microorganism capable of producing the enzyme, and a medium containing fructosyl lysine and/or fructosyl N.sup..alpha. -Z-lysine for screening and/or culturing a microorganism capable of producing a fructosyl amino acid oxidase is provided.

5. 发明授权

US5712138A Fructosyl amino acid oxidase 失效
标题翻译：果糖基氨基酸氧化酶
公开(公告)号：US5712138A
公开(公告)日：1998-01-27
申请号：US536190
申请日：1995-09-29
申请人： Nobuo Kato , Yasuyoshi Sakai , Yoshiki Tani , Toshikatsu Sakai , Kaori Ishimaru
发明人： Nobuo Kato , Yasuyoshi Sakai , Yoshiki Tani , Toshikatsu Sakai , Kaori Ishimaru
IPC分类号： C12N9/06 , C12Q1/26 , C12N9/02
CPC分类号： C12N9/0022 , C12Q1/26
摘要： A novel fructosyl amino acid oxidase derived from genus Fusarium, which is active on both of fructosyl lysine and fructosyl valine, a process for producing the enzyme, an assay of an amadori compound using the enzyme, a reagent or a kit containing the enzyme is provided.
摘要翻译：提供了一种来源于镰刀菌属的新型果糖基氨基酸氧化酶，其在果糖基赖氨酸和果糖基缬氨酸两者上均有活性，提供了该酶的制备方法，使用该酶的阿马多利化合物的测定法，含该酶的试剂或试剂盒。

6. 发明授权

US06423510B1 Candida boidini dihydroxyacetone synthase promoter 失效
标题翻译：念珠菌boidini二羟基丙酮合酶启动子
公开(公告)号：US06423510B1
公开(公告)日：2002-07-23
申请号：US09622540
申请日：2000-08-18
申请人： Toshihiro Komeda , Keiji Kondo , Nobuo Kato , Yasuyoshi Sakai
发明人： Toshihiro Komeda , Keiji Kondo , Nobuo Kato , Yasuyoshi Sakai
IPC分类号： C12P2106
CPC分类号： C12N15/815 , C12N9/1022
摘要： This invention relates to a promoter for dihydroxyacetone synthase gene from Candida boidinii; an expression cassette or vector comprising the promoter and a heterologous gene; a transformant transformed with this vector; and a method for producing an expression product of the heterologous gene which comprises culturing the transformant to express the heterologous gene.
摘要翻译：本发明涉及来自布氏念珠菌的二羟基丙酮合成酶基因的启动子; 包含启动子和异源基因的表达盒或载体; 用该载体转化的转化体; 以及用于产生异源基因的表达产物的方法，其包括培养转化体以表达异源基因。

7. 发明授权

US5824527A Fructosyl amino acid oxidase and process for producing the same 失效
标题翻译：果糖基氨基酸氧化酶及其制备方法
公开(公告)号：US5824527A
公开(公告)日：1998-10-20
申请号：US630175
申请日：1996-04-11
申请人： Nobuo Kato , Yasuyoshi Sakai , Yoshiki Tani , Masayuki Yagi , Fumiyo Funatsu
发明人： Nobuo Kato , Yasuyoshi Sakai , Yoshiki Tani , Masayuki Yagi , Fumiyo Funatsu
IPC分类号： C12N9/06 , C12N9/02 , C12Q1/26
CPC分类号： C12N9/0022 , C12R1/80
摘要： A novel fructosyl amino acid oxidase derived from genus Penicillium, which is active on both of fructosyl lysine and fructosyl valine, a process for producing the enzyme, an assay of an amadori compound using the enzyme, a reagent or a kit containing the enzyme is provided.
摘要翻译：提供了一种来源于青霉属的新型果糖基氨基酸氧化酶，其在果糖基赖氨酸和果糖基缬氨酸两者上均有活性，提供了生产酶的方法，使用该酶的阿马多利化合物的测定法，含有该酶的试剂或试剂盒。

8. 发明授权

US06001590A Promoter and terminator sequences of formate dehydrogenase gene of Candida boidinii 失效
标题翻译：博伊丁假丝酵母的甲酸脱氢酶基因的启动子和终止子序列
公开(公告)号：US06001590A
公开(公告)日：1999-12-14
申请号：US817926
申请日：1997-05-09
申请人： Toshihiro Komeda , Hisako Suda , Yukio Tamai , Akihiro Iwamatsu , Nobuo Kato , Yasuyoshi Sakai
发明人： Toshihiro Komeda , Hisako Suda , Yukio Tamai , Akihiro Iwamatsu , Nobuo Kato , Yasuyoshi Sakai
IPC分类号： C12N1/15 , C12N9/02 , C12N15/81 , C12N15/31 , C12P21/00
CPC分类号： C12N9/0008 , C12N15/815
摘要： A promoter for a formate dehydrogenase gene from Candida boidinii, substantially comprising a 190 bp or more continuous nucleotide sequence selected from the nucleotide sequence of SEQ ID NO:1; a promoter for a formate dehydrogenase gene from Candida boidinii, substantially comprising the nucleotide sequence of SEQ ID NO:1, 48, 49 or 50; a terminator for a formate dehydrogenase gene from Candida boidinii, substantially comprising the nucleotide sequence of SEQ ID NO:2; a gene expression cassette comprising said promoter, a heterologous gene and said terminator; a recombinant expression vector comprising said gene expression cassette; a transformant transformed with said recombinant expression vector; a process for producing an expression product of a heterologous gene, which comprises culturing said transformant and recovering an expression product of a heterologous gene from the culture.
摘要翻译： PCT No.PCT / JP96 / 02597 371日期：1997年5月9日 102（e）日期1997年5月9日PCT提交1996年9月12日PCT公布。公开号WO97 / 10345 日期：1997年3月20日来自布氏念珠菌的甲酸脱氢酶基因的启动子，基本上包含选自SEQ ID NO：1的核苷酸序列的190bp以上的连续核苷酸序列; 来自布氏念珠菌的甲酸脱氢酶基因的启动子，基本上包含SEQ ID NO：1,49,49或50的核苷酸序列; 来自布氏念珠菌的甲酸脱氢酶基因的终止子，基本上包含SEQ ID NO：2的核苷酸序列; 包含所述启动子，异源基因和所述终止子的基因表达盒; 包含所述基因表达盒的重组表达载体; 用所述重组表达载体转化的转化体; 用于产生异源基因的表达产物的方法，其包括培养所述转化体并从培养物中回收异源基因的表达产物。

9. 发明授权

US5965426A Protein disulfide isomerase gene derived from strain of methylotrophic yeast 失效
标题翻译：衍生自甲基营养酵母菌株的蛋白质二硫键异构酶基因
公开(公告)号：US5965426A
公开(公告)日：1999-10-12
申请号：US923536
申请日：1997-09-04
申请人： Yasuyoshi Sakai , Nobuo Kato , Yuji Shibano
发明人： Yasuyoshi Sakai , Nobuo Kato , Yuji Shibano
IPC分类号： C12N9/90 , C12N15/61 , C12N15/81 , C07H21/04 , C12N1/20 , C12N15/00
CPC分类号： C12N15/815 , C12N9/90
摘要： A protein derived from a strain of methylotrophic yeast which has a protein disulfide isomerase activity having the amino acid sequence as set forth in SEQ ID No. 1, or protein in which the amino acid sequence has been modified by deletion or addition of one or a few amino acids, or substitution with other amino acid(s) and which has a protein disulfide isomerase activity; and a process for production thereof.
摘要翻译：衍生自具有SEQ ID No.1所示的氨基酸序列的蛋白质二硫键异构酶活性的甲基营养酵母菌株的蛋白质或其氨基酸序列已经通过缺失或添加一个或多个氨基酸序列而被修饰的蛋白质少量氨基酸，或与其他氨基酸取代并具有蛋白质二硫键异构酶活性; 及其制造方法。

10. 发明授权

US06171828B2 Method for culturing microorganisms having a methanol metabolic pathway 失效
标题翻译：培养具有甲醇代谢途径的微生物的方法
公开(公告)号：US06171828B2
公开(公告)日：2001-01-09
申请号：US08811024
申请日：1997-03-04
申请人： Koji Magota , Tomohiro Rogi , Yasuyoshi Sakai , Nobuo Kato
发明人： Koji Magota , Tomohiro Rogi , Yasuyoshi Sakai , Nobuo Kato
IPC分类号： C12P102
CPC分类号： C12N9/60 , C12N15/815
摘要： The present invention discloses a method for culturing microorganisms having a methanol metabolic pathway in which an expression unit is introduced that comprises a target gene linked downstream from a promoter that can be induced by methanol; wherein, during the culturing period, and including the period during which methanol is continuously or periodically added, the rate of addition is adjusted to a rate equal to or less than the maximum methanol consumption rate of said microorganisms.
摘要翻译：本发明公开了一种用于培养具有甲醇代谢途径的微生物的方法，其中引入了表达单元，所述表达单元包含与可由甲醇诱导的启动子下游连接的靶基因; 其中，在培养期间，包括连续或周期性添加甲醇的期间，将添加速度调整为等于或小于所述微生物的最大甲醇消耗速率的速率。

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