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1. 发明授权

US10837022B1 Recombinant microorganisms with mixed sugar utilization 有权
公开(公告)号：US10837022B1
公开(公告)日：2020-11-17
申请号：US15708262
申请日：2017-09-19
申请人： The Board of Trustees of the University of Illinois
发明人： Yong-Su Jin , Stephan Thomas Lane , Haiqing Xu , Soo-Rin Kim
IPC分类号： C12N15/81 , C12N1/26 , C12N9/12 , C12P7/06
CPC分类号： C12N15/81 , C12N1/26 , C12N9/1258 , C12P7/06
摘要： The present disclosure provides recombinant microorganisms capable of sugar co-utilization, the recombinant microorganism comprising a genetically altered microorganism (e.g. S. cerevisiae) having a lack of, or reduced expression of, or expression of truncated or mutated forms of at least one polypeptide selected from Hxk1, Hxk2, and Glk1. Reduction of the expression or biological activity of Hxk1, Hxk2 and Glk1 leads to reduction in the glucose consumption pathway, allowing the microorganism to co-utilize multiple sugars (e.g. glucose, xylose, galactose) at an improved rate.

2. 发明授权

US09869166B2 Microbial enhanced oil recovery method 有权
公开(公告)号：US09869166B2
公开(公告)日：2018-01-16
申请号：US14917871
申请日：2014-09-11
申请人： GEO FOSSIL FUELS, LLC
发明人： William J. Kohr , David J. Galgoczy , Zhaoduo Zhang
IPC分类号： E21B43/20 , C09K8/582 , C12N1/26 , C12P19/06 , C12P7/64
CPC分类号： E21B43/20 , C09K8/582 , C12N1/26 , C12P7/6463 , C12P19/06
摘要： The present invention provides methods for increasing the viscosity of the drive fluid for displacing oil from a subterranean formation by the use of microorganisms selected or modified for the ability to produce cell free polymers without the formation of any significant bioplugging biofilm or capsule.

3. 发明申请

US20070225355A1 Dietary Compositions for Enhancing Metabolism and Reducing Reactive Oxygen Species 审中-公开
标题翻译：用于增强代谢和减少活性氧物质的膳食组合物
公开(公告)号：US20070225355A1
公开(公告)日：2007-09-27
申请号：US11571321
申请日：2005-06-23
申请人： Cheng Liu
发明人： Cheng Liu
IPC分类号： A61K31/385 , C07D339/04 , C12N1/02 , C12N1/26
CPC分类号： A61K31/385 , A23K20/105 , A23K20/111 , A23K20/121 , A23K40/10 , A23K40/30
摘要： This invention relates to dietary compositions and to methods for modifying cellular metabolism, metabolic production of reactive oxygen species and the resulting level of reactive oxygen species. The invention is drawn to a composition comprising a combination of carnitine, lipoic acid and polyphenol, which has the effect of enhancing metabolism and reducing oxygen species at the same time. When the composition is fed to older animals, the animals will have improved metabolism at the cellular level and a reduction of oxidative stress. The invention is also drawn to a method of oral administration of carnitine, lipoic acid and polyphenol to a mammalian host, at an effective dose necessary to affect enhanced metabolic processes and reduced oxygen species in animals including humans.
摘要翻译：本发明涉及饮食组合物以及用于改变细胞代谢，活性氧的代谢产生和所产生的活性氧水平的方法。本发明涉及包含肉碱，硫辛酸和多酚的组合的组合物，其具有同时增强代谢和还原氧的作用。当将组合物喂养给较老的动物时，动物将在细胞水平上具有改善的代谢并降低氧化应激。本发明还涉及一种将哺乳动物宿主肉碱，硫辛酸和多酚口服给药的方法，所述方法是以包括人在内的动物的增强代谢过程和还原氧物质所必需的有效剂量。

4. 发明授权

US6051420A Method for the decontamination of soil containing solid organic explosives therein 失效
标题翻译：其中含有固体有机炸药的土壤去污的方法
公开(公告)号：US6051420A
公开(公告)日：2000-04-18
申请号：US82421
申请日：1998-05-20
申请人： Corey W. Radtke , Francisco F. Roberto
发明人： Corey W. Radtke , Francisco F. Roberto
IPC分类号： B09C1/10 , C12N1/20 , C12N1/26 , C12N1/32 , C12S9/00 , C12S13/00 , B09B3/00
CPC分类号： C12N1/20 , B09C1/10 , C12N1/26 , C12N1/32 , Y02W30/43 , Y02W30/47
摘要： An efficient method for decontaminating soil containing organic explosives ("TNT" and others) in the form of solid portions or chunks which are not ordinarily subject to effective bacterial degradation. The contaminated soil is treated by delivering an organic solvent to the soil which is capable of dissolving the explosives. This process makes the explosives more bioavailable to natural bacteria in the soil which can decompose the explosives. An organic nutrient composition is also preferably added to facilitate decomposition and yield a compost product. After dissolution, the explosives are allowed to remain in the soil until they are decomposed by the bacteria. Decomposition occurs directly in the soil which avoids the need to remove both the explosives and the solvents (which either evaporate or are decomposed by the bacteria). Decomposition is directly facilitated by the solvent pre-treatment process described above which enables rapid bacterial remediation of the soil.
摘要翻译：一种有效的方法，用于净化含有有机爆炸物（“TNT”等）的土壤，其形式为固体部分或块，通常不会有效地进行细菌降解。污染的土壤通过将有机溶剂输送到能够溶解爆炸物的土壤来处理。这个过程使得爆炸物对土壤中能分解爆炸物的天然细菌更具生物利用度。还优选加入有机营养组合物以促进分解并产生堆肥产物。溶解后，允许爆炸物留在土壤中直至细菌分解。分解直接发生在土壤中，避免需要去除爆炸物和溶剂（其被蒸发或被细菌分解）。通过上述溶剂预处理方法直接促进分解，其能够快速对土壤进行细菌修复。

5. 发明授权

US5962306A Method of determining the presence or absence of a nonparaffinophilic microorganism in a specimen and an associated apparatus 失效
标题翻译：确定样品和相关装置中存在或不存在非亲油性微生物的方法
公开(公告)号：US5962306A
公开(公告)日：1999-10-05
申请号：US3833
申请日：1998-01-07
申请人： Robert-A. Ollar , Mitchell S. Felder
发明人： Robert-A. Ollar , Mitchell S. Felder
IPC分类号： C12M1/00 , C12M1/24 , C12M1/34 , C12N1/26 , C12Q1/04 , C12Q1/08 , C12Q1/14 , C12Q1/24
CPC分类号： C12Q1/04 , C12M23/08 , C12N1/26 , C12Q1/14
摘要： A method of determining the presence or absence of a nonparaffinophilic microorganism in a specimen taken from a patient includes providing a receptacle containing an aqueous solution that does not contain a carbon source and inoculating the aqueous solution with the specimen. A slide having bound thereto a carbon source is placed into the receptacle. By analyzing the slide after exposure to the specimen, the presence or absence of a nonparaffinophilic microorganism in the specimen can be determined. An associated apparatus is also disclosed.
摘要翻译：确定从患者取出的样本中存在或不存在非石蜡嗜性微生物的方法包括提供含有不含碳源的水溶液的容器并且将样品接种在水溶液中。将具有碳源的滑块放入容器中。通过在暴露于样品后分析载玻片，可以确定样品中存在或不存在非嗜性微生物。还公开了一种相关装置。

6. 发明授权

US5780290A Non-polluting compositions to degrade hydrocarbons and microorganisms for use thereof 失效
标题翻译：用于降解烃和微生物的无污染组合物
公开(公告)号：US5780290A
公开(公告)日：1998-07-14
申请号：US461754
申请日：1995-06-05
申请人： Eugene Rosenberg , Eliora Z. Ron
发明人： Eugene Rosenberg , Eliora Z. Ron
IPC分类号： A62D3/00 , A62D3/02 , B09C1/10 , C02F1/68 , C02F3/34 , C12N1/20 , C12N1/26 , C12N11/08 , C12R1/01 , C12R1/05 , C12R1/38 , C12N1/00 , C12N1/38
CPC分类号： A62D3/02 , B09C1/10 , C02F1/682 , C02F3/34 , C02F3/344 , C12N1/26 , A62D2101/20 , Y10S210/922 , Y10S435/821 , Y10S435/826 , Y10S435/834
摘要： The present invention relates to compositions containing bacteria capable of degrading hydrocarbons, such as petroleum or petroleum products and methods for their use.
摘要翻译：本发明涉及含有能够降解烃类的细菌的组合物，例如石油或石油产品及其使用方法。

7. 发明授权

US5698414A Method and apparatus for testing paraffinophilic microorganisms for antimicrobial agent sensitivity 失效
标题翻译：用于测试抗菌剂敏感性的石蜡微生物的方法和设备
公开(公告)号：US5698414A
公开(公告)日：1997-12-16
申请号：US538899
申请日：1995-10-04
申请人： Robert-A Ollar
发明人： Robert-A Ollar
IPC分类号： C12M1/00 , C12M1/24 , C12M1/34 , C12Q1/04 , C12Q1/08 , C12Q1/14 , C12Q1/18 , C12N1/26
CPC分类号： C12M23/08 , C12Q1/18 , G01N2333/35
摘要： A method of determining the sensitivity of at least one paraffinophilic microorganism from a specimen obtained from a patient to different antimicrobial agents and predetermined quantities thereof includes providing at least one receptacle containing an aqueous solution and then inoculating the solution with the specimen. The method then includes placing into the receptacle (i) a paraffin coated slide to bait the at least one paraffinophilic microorganism and (ii) a predetermined quantity of an antimicrobial agent to be tested. The slide is then observed for paraffinophilic microorganism growth or lack thereof to determine whether the predetermined quantity of the antimicrobial agent is effective in inhibiting growth of the paraffinophilic microorganisms on the slide. An associated apparatus is also provided.
摘要翻译：确定从患者获得的样品至不同抗微生物剂及其预定量的至少一种嗜
温微生物的灵敏度的方法包括提供至少一个含有水溶液的容器，然后将样品与溶液接种。该方法然后包括将容器（i）置于石蜡包裹的载玻片上以诱饵至少一种嗜
温微生物和（ii）预定量的待测试的抗微生物剂。然后观察载玻片以进行石蜡微生物生长或缺乏，以确定预定量的抗微生物剂是否有效抑制载玻片上的嗜石微生物的生长。还提供了一种相关联的装置。

8. 发明授权

US5672503A Plant growth establishment and agents for use therein 失效
标题翻译：植物生长建立和其中使用的试剂
公开(公告)号：US5672503A
公开(公告)日：1997-09-30
申请号：US470839
申请日：1995-06-06
申请人： Beverley Janice Nairn , Richard Hubert Furneaux
发明人： Beverley Janice Nairn , Richard Hubert Furneaux
IPC分类号： A01H4/00 , C08B37/00 , C12N5/00 , C12N5/04 , C12N1/26 , C12N1/38
CPC分类号： A01H4/001 , C08B37/0039 , C12N5/0025
摘要： Method for inhibiting vitrification of plants of the Pinus species and Eucalyptus species. In the method, anti-vitrification agents containing one or more sulfated polysaccharides are used. The sulfated polysaccharides are preferably free of toxic components. Tissue culture media is also disclosed.
摘要翻译：抑制樟子类和桉树种植物玻璃化的方法。在该方法中，使用含有一种或多种硫酸化多糖的抗玻璃化剂。硫酸化多糖优选不含有毒成分。组织培养基也被披露。

9. 发明授权

US5508194A Nutrient medium for the bioremediation of polycyclic aromatic hydrocarbon-contaminated soil 失效
标题翻译：用于多环芳烃污染土壤生物修复的营养培养基
公开(公告)号：US5508194A
公开(公告)日：1996-04-16
申请号：US444161
申请日：1995-05-18
申请人： Sunggyu Lee , Teresa J. Cutright
发明人： Sunggyu Lee , Teresa J. Cutright
IPC分类号： B09C1/10 , C02F3/34 , C12P1/00 , C12P39/00 , C12S9/00 , C12N1/20 , C12N1/26
CPC分类号： C12P39/00 , B09C1/10 , C02F3/34 , C12P1/00 , Y10S435/824 , Y10S435/863
摘要： A mixed bacteria culture for biodegrading polycyclic aromatic hydrocarbon contaminants includes Achromobacter sp. and Mycobacterium sp. which have been grown together and gradually acclimated to utilize polycyclic aromatic hydrocarbons as a primary food source. The mixed bacteria culture can be utilized for in situ or ex situ bioremediation of contaminated soil, or in any of various conventional bioreactors to treat contaminated liquids such as landfill leachates, groundwater or industrial effluents. The bacteria, the nutrients used to sustain growth of the bacteria, and the products of the biodegradation of the polycyclic aromatic or other hydrocarbons are all substantially harmless to the environment. The mixed bacteria can be utilized in the presence of oxygen, or hydrogen peroxide can be used alone or in combination with oxygen as an effective alternative electron acceptor. The mixed bacteria culture of Achromobacter sp. and Mycobacterium sp. provides an environmentally safe and affordable means for rapidly and effectively eliminating a variety of polycyclic aromatic hydrocarbon contaminants from the environment.
摘要翻译：用于生物降解多环芳烃污染物的混合细菌培养物包括无色杆菌属。和分枝杆菌属它们一起种植，逐渐适应利用多环芳烃作为主要食物来源。混合细菌培养物可用于污染土壤或任何各种常规生物反应器的原位或非原生境生物修复，以处理污染液体，如垃圾渗滤液，地下水或工业废水。细菌，用于维持细菌生长的营养物质以及多环芳烃或其他烃的生物降解产物对环境基本无害。混合的细菌可以在氧的存在下使用，或者可以单独或与氧组合使用过氧化氢作为有效的替代电子受体。无色杆菌混合细菌培养和分枝杆菌属提供了一种环境安全和经济实惠的手段，用于快速有效地从环境中消除各种多环芳烃污染物。

10. 发明授权

US4985363A Microorganism capable of growing in 50% or more organic solvent 失效
标题翻译：能够在50％以上有机溶剂中生长的微生物
公开(公告)号：US4985363A
公开(公告)日：1991-01-15
申请号：US163576
申请日：1988-03-03
申请人： Akira Inoue , Kouki Horikoshi
发明人： Akira Inoue , Kouki Horikoshi
IPC分类号： C12N1/20 , C12N1/26 , C12N1/32
CPC分类号： C12R1/38 , C12N1/20 , C12N1/26 , C12N1/32 , Y10S435/874 , Y10S435/877
摘要： New microorganisms belonging to Pseudomonas putida or Pseudomonas sp., which are isolated from soil and have tolerance to one or more of hydrocarbons, alcohols, ethers, ketones and their derivatives or their mixture. These new microorganisms can be used in the fields of bioreactor, liquid-waste treatment, protein engineering, etc.

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