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    • 5. 发明授权
    • Yeast vector comprising a shortened promoter sequence
    • 包含缩短的启动子序列的酵母载体
    • US06284534B1
    • 2001-09-04
    • US09242690
    • 1999-02-23
    • Keiji KondoYutaka Miura
    • Keiji KondoYutaka Miura
    • C12N1581
    • C12N9/2411C07K14/43C12N9/2408C12N15/815C12N15/905C12Y302/01001
    • An object of the present invention is to provide a vector which can be integrated into a yeast chromosome in a high number of copies. Another object of the present invention is to provide a modified vector which can be integrated into the yeast chromosome in a high number of copies and of which expression units stably maintain on the chromosome. The vector according to the present invention comprises a marker gene for selecting transformants, a shortened promoter sequence which is operably linked to the marker gene and a sequence homologous to the chromosomal DNA of Candida utilis, and optionally a heterologous gene or a gene derived from C. utilis, wherein the vector is linearized by cleaving within said homologous DNA sequence or at both ends of the homologous DNA sequence with restriction enzymes, and wherein the heterologous gene or the gene derived from C. utilis can be integrated into the chromosomal DNA of C. utilis by homologous recombination.
    • 本发明的目的是提供一种能够以大量拷贝结合到酵母染色体中的载体。 本发明的另一个目的是提供一种修饰的载体,其能够以大量的拷贝整合到酵母染色体中,并且其表达单位稳定地保持在染色体上。 根据本发明的载体包含用于选择转化体的标记基因,可操作地连接到标记基因的缩短的启动子序列和与产朊假丝酵母的染色体DNA同源的序列,以及任选的异源基因或衍生自C的基因 其中载体通过在所述同源DNA序列内切割或用限制性内切酶在同源DNA序列的两端进行线性化,其中异源基因或源自产朊酵母的基因可以整合入C的染色体DNA中 通过同源重组产生。