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    • 6. 发明授权
    • Hexulose phosphate isomerase gene
    • 己糖基磷酸异构酶基因
    • US06331428B1
    • 2001-12-18
    • US09185501
    • 1998-11-04
    • Nobuo Kato
    • Nobuo Kato
    • C12N990
    • C12N9/90
    • Hexulose phosphate synthase was purified from Mycobacterium gastri to determine an amino acid sequence of the enzyme. Oligonucleotide primers to be used for PCR were synthesized on the basis of obtained amino acid sequence information. A DNA fragment, which was amplified by performing PCR by using a template of genomic DNA prepared from Mycobacterium gastri, was used as a probe so that colony hybridization was carried out with respect to a library comprising fragments obtained by digesting Mycobacterium gastri genomic DNA with PstI to obtain a positive clone. Thus, the DNA fragment which contains ORF encoding hexulose phosphate synthase was obtained. Furthermore, the DNA fragment cloned contained other ORF(ORF-1). The expression system of ORF-1 was constructed (pT-HPIS-1), and it was introduced into Escherichia coli cell. After the induction of the expression of ORF-1, the hexulose phosphate isomerase activity was found in the extract of cells harvoring pT-HPIS-1. Thus, gene, which coded for hexulose phosphate isomerase, was obtained together with ORF which coded for hexulose phosphate synthase.
    • 从胃分枝杆菌中纯化己糖磷酸合成酶以确定该酶的氨基酸序列。 基于获得的氨基酸序列信息合成用于PCR的寡核苷酸引物。 使用通过使用由分枝杆菌(Gastri)制备的基因组DNA的模板进行PCR扩增的DNA片段作为探针,以便对包含通过用PstI消化胃分枝杆菌基因组DNA获得的片段的文库进行集落杂交 以获得阳性克隆。 因此,获得含有编码磷酸内酯磷酸合成酶的ORF的DNA片段。 此外,克隆的DNA片段含有其他ORF(ORF-1)。 构建了ORF-1的表达系统(pT-HPIS-1),并将其导入大肠杆菌细胞。 在诱导ORF-1的表达后,在含有pT-HPIS-1的细胞提取物中发现己糖基磷酸异构酶活性。 因此,编码己糖基磷酸异构酶的基因与编码磷酸内酯磷酸合成酶的ORF一起获得。
    • 7. 发明授权
    • High-pressure fuel injection pipe for diesel engine
    • 柴油机高压燃油喷射管
    • US06286556B1
    • 2001-09-11
    • US09564514
    • 2000-05-04
    • Nobuo Kato
    • Nobuo Kato
    • F16L918
    • F02M55/005F02M55/02
    • A high-pressure fuel injection pipe for a diesel engine includes a fuel pipe main body with opposed first and second ends. First and second fastening nuts are mounted over the first and second ends. A first unitary outer pipe is mounted over a portion of the fuel pipe main body and is mounted to the first fastening nut. A second unitary outer pipe is mounted over a portion of the fuel pipe main body and has an end mounted to the second fastening nut. The end of the second outer pipe is spaced from the second fastening nut is expanded circumferentially and telescoped over a portion of the first outer pipe. A sealing ring is sealingly engaged between the first and second outer pipes.
    • 用于柴油发动机的高压燃料喷射管包括具有相对的第一和第二端的燃料管主体。 第一和第二紧固螺母安装在第一和第二端上。 第一单体外管安装在燃料管主体的一部分上并安装在第一紧固螺母上。 第二单体外管安装在燃料管主体的一部分上,并且具有安装到第二紧固螺母的端部。 第二外管的端部与第二紧固螺母间隔开,其周向地伸展并且伸缩在第一外管的一部分上。 密封环密封地接合在第一和第二外管之间。
    • 10. 发明授权
    • Controller and control method
    • 控制器和控制方法
    • US5839111A
    • 1998-11-17
    • US771682
    • 1996-12-23
    • Jochiku MuraokaNobuo Kato
    • Jochiku MuraokaNobuo Kato
    • G10K15/04H04L12/40H04N7/10H04N21/4363G10L3/00
    • H04N7/106
    • An audio/video (AV) information transaction system includes AV appliances installed in individual rooms, controllers associated with the AV appliances and a host computer which is linked with the controllers through a local area network (LAN), thereby forming a computer network. The host computer transfers control programs to the controllers. A controller in a room which runs the received control program sends a command signal of the demand of AV output reception to other controller in other room over the LAN cable, and the receiving controller operates the associated AV appliance and delivers the output AV signal to the demanding controller over the LAN cable.
    • 音频/视频(AV)信息交易系统包括安装在单独房间中的AV设备,与AV设备相关联的控制器和通过局域网(LAN)与控制器链接的主计算机,从而形成计算机网络。 主机将控制程序传送到控制器。 运行接收到的控制程序的房间中的控制器通过LAN电缆向另一房间的其他控制器发送AV输出接收要求的命令信号,并且接收控制器操作相关联的AV设备并将输出AV信号传送到 通过LAN电缆来控制控制器。