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1. 发明申请

US20090081726A1 Recombinant Microorganism 有权
标题翻译：重组微生物
公开(公告)号：US20090081726A1
公开(公告)日：2009-03-26
申请号：US11722162
申请日：2005-12-20
申请人： Takeko Kodama , Keiji Endo , Katsuya Ozaki , Junichi Sekiguchi
发明人： Takeko Kodama , Keiji Endo , Katsuya Ozaki , Junichi Sekiguchi
IPC分类号： C12P21/04 , C12N1/21
CPC分类号： C12P21/02 , C07K14/32 , C12N9/54 , C12N15/75
摘要： A host microorganism capable of increasing productivity of a protein or polypeptide, a recombinant microorganism obtained by introducing a gene encoding a protein or polypeptide into the host microorganism, and a method for producing a protein or polypeptide using the recombinant microorganism are provided.Also provided is a recombinant microorganism obtained by introducing into a host microorganism a gene encoding a heterologous protein or polypeptide, wherein in said host microorganism the Bacillus subtilis aprX gene or a gene corresponding to the aprX gene has been deleted or knocked out, and a method for producing a protein or polypeptide using the recombinant microorganism.
摘要翻译：提供能够提高蛋白质或多肽的生产力的宿主微生物，通过将编码蛋白质或多肽的基因导入宿主微生物而获得的重组微生物，以及使用该重组微生物产生蛋白质或多肽的方法。还提供了通过向宿主微生物中导入编码异源蛋白质或多肽的基因而获得的重组微生物，其中在所述宿主微生物中，枯草芽孢杆菌aprX基因或与aprX基因相对应的基因已被缺失或敲除，用于使用重组微生物产生蛋白质或多肽。

2. 发明授权

US07829322B2 Recombinant microorganism comprising inactivation of the AprX gene 有权
标题翻译：包含AprX基因失活的重组微生物
公开(公告)号：US07829322B2
公开(公告)日：2010-11-09
申请号：US11722162
申请日：2005-12-20
申请人： Takeko Kodama , Keiji Endo , Katsuya Ozaki , Junichi Sekiguchi
发明人： Takeko Kodama , Keiji Endo , Katsuya Ozaki , Junichi Sekiguchi
IPC分类号： C12N1/21 , C12N15/00 , C12N15/57 , C12N15/75
CPC分类号： C12P21/02 , C07K14/32 , C12N9/54 , C12N15/75
摘要： A host microorganism capable of increasing productivity of a protein or polypeptide, a recombinant microorganism obtained by introducing a gene encoding a protein or polypeptide into the host microorganism, and a method for producing a protein or polypeptide using the recombinant microorganism are provided.Also provided is a recombinant microorganism obtained by introducing into a host microorganism a gene encoding a heterologous protein or polypeptide, wherein in said host microorganism the Bacillus subtilis aprX gene or a gene corresponding to the aprX gene has been deleted or knocked out, and a method for producing a protein or polypeptide using the recombinant microorganism.
摘要翻译：提供能够提高蛋白质或多肽的生产力的宿主微生物，通过将编码蛋白质或多肽的基因导入宿主微生物而获得的重组微生物，以及使用该重组微生物产生蛋白质或多肽的方法。还提供了通过向宿主微生物中导入编码异源蛋白质或多肽的基因而获得的重组微生物，其中在所述宿主微生物中，枯草芽孢杆菌aprX基因或与aprX基因相对应的基因已被缺失或敲除，用于使用重组微生物产生蛋白质或多肽。

3. 发明申请

US20090170154A1 Mutant bacterium belonging to the genus bacillus 有权
标题翻译：属于杆菌属的突变细菌
公开(公告)号：US20090170154A1
公开(公告)日：2009-07-02
申请号：US10590275
申请日：2005-03-04
申请人： Keiji Endo , Katsuya Ozaki
发明人： Keiji Endo , Katsuya Ozaki
IPC分类号： C12P21/00 , C12N1/21 , C12N15/87
CPC分类号： C12N9/2417 , C12N9/2437 , C12N9/54 , C12Y302/01004
摘要： The present invention provides a mutant Bacillus bacterium capable of enhancing productivity of proteins or polypeptides, a recombinant microorganism produced by introducing genes encoding heterologous proteins or polypeptides into the mutant Bacillus bacterium, and a method for producing proteins or polypeptides by use of the recombinant microorganism.A mutant Bacillus bacterium which has, on the genome or plasmid thereof, DNA having a promoter sequence which is recognized and transcribed specifically during the sporulation stage and which is ligated to an upstream end of a sigA gene or a gene equivalent thereto, a recombinant microorganism produced by introducing genes encoding heterologous proteins or polypeptides into the mutant Bacillus bacterium, and a method for producing proteins or polypeptides by use of the recombinant microorganism.
摘要翻译：本发明提供能够提高蛋白质或多肽生产力的突变芽孢杆菌属细菌，通过将编码异源蛋白质或多肽的基因导入突变芽孢杆菌属细菌产生的重组微生物，以及通过使用重组微生物产生蛋白质或多肽的方法。一种突变芽孢杆菌属细菌，其在基因组或质粒上具有在孢子形成阶段中特异性识别并转录并与sigA基因或其等同基因相连的基因的上游端的启动子序列的DNA，重组微生物通过将编码异源蛋白质或多肽的基因导入突变芽孢杆菌属细菌而产生，以及通过使用重组微生物产生蛋白质或多肽的方法。

4. 发明申请

US20080026976A1 Highly productive alpha-amylases 审中-公开
标题翻译：高生产力的α-淀粉酶
公开(公告)号：US20080026976A1
公开(公告)日：2008-01-31
申请号：US11590769
申请日：2006-11-01
申请人： Hiroyuki Araki , Keiji Endo , Hiroshi Hagihara , Kazuaki Igarashi , Yasuhiro Hayashi , Katsuya Ozaki
发明人： Hiroyuki Araki , Keiji Endo , Hiroshi Hagihara , Kazuaki Igarashi , Yasuhiro Hayashi , Katsuya Ozaki
IPC分类号： C11D3/386 , C07H21/00 , C12N1/00 , C12N15/63 , C12N9/26 , C12P21/00
CPC分类号： C12N9/2417 , C11D3/386
摘要： The invention relates to mutant α-amylases that may be produced at high yield from recombinant microorganisms.
摘要翻译：本发明涉及可以从重组微生物以高产率产生的突变型α-淀粉酶。

5. 发明授权

US07855065B2 Mutant bacterium belonging to the genus Bacillus 有权
标题翻译：属于芽孢杆菌属的突变细菌
公开(公告)号：US07855065B2
公开(公告)日：2010-12-21
申请号：US10590275
申请日：2005-03-04
申请人： Keiji Endo , Katsuya Ozaki
发明人： Keiji Endo , Katsuya Ozaki
IPC分类号： C12N1/20 , C12N15/74 , C12N15/75 , C12P21/04
CPC分类号： C12N9/2417 , C12N9/2437 , C12N9/54 , C12Y302/01004
摘要： The present invention provides a mutant Bacillus bacterium capable of enhancing productivity of proteins or polypeptides, a recombinant microorganism produced by introducing genes encoding heterologous proteins or polypeptides into the mutant Bacillus bacterium, and a method for producing proteins or polypeptides by use of the recombinant microorganism. A mutant Bacillus bacterium which has, on the genome or plasmid thereof, DNA having a promoter sequence which is recognized and transcribed specifically during the sporulation stage and which is ligated to an upstream end of a sigA gene or a gene equivalent thereto, a recombinant microorganism produced by introducing genes encoding heterologous proteins or polypeptides into the mutant Bacillus bacterium, and a method for producing proteins or polypeptides by use of the recombinant microorganism.
摘要翻译：本发明提供能够提高蛋白质或多肽生产力的突变芽孢杆菌属细菌，通过将编码异源蛋白质或多肽的基因导入突变芽孢杆菌属细菌产生的重组微生物，以及通过使用重组微生物产生蛋白质或多肽的方法。一种突变芽孢杆菌属细菌，其在基因组或质粒上具有在孢子形成阶段中特异性识别并转录并与sigA基因或其等同基因相连的基因的上游端的启动子序列的DNA，重组微生物通过将编码异源蛋白质或多肽的基因导入突变芽孢杆菌属细菌而产生，以及通过使用重组微生物产生蛋白质或多肽的方法。

6. 发明授权

US07585674B2 Host microorganisms 失效
标题翻译：宿主微生物
公开(公告)号：US07585674B2
公开(公告)日：2009-09-08
申请号：US10479214
申请日：2002-05-28
申请人： Kazuhisa Sawada , Keiji Endo , Tadahiro Ozawa , Masatoshi Tohata , Katsuya Ozaki
发明人： Kazuhisa Sawada , Keiji Endo , Tadahiro Ozawa , Masatoshi Tohata , Katsuya Ozaki
IPC分类号： C12N5/06 , G01N33/53
CPC分类号： C07K14/32 , C07K2319/00 , C12N1/20
摘要： A microorganism wherein one or more genes selected from the group of genes participating in sporulation in the middle to late stages of sporulation have been deleted or inactivated; and a process for producing a target product (a protein) by use the microorganism. No spore is formed when this microorganism is employed, thereby enabling production of a target product (a protein) while decreasing energy loss, production of a by-product and specific production speed to decrease unnecessary consumption of a medium. Moreover, the production period can be prolonged, whereby the target product (the protein) can be produced efficiently.
摘要翻译：一种微生物，其中一个或多个选自参与孢子形成中期至晚期孢子形成的基因的基因已被删除或失活; 以及通过使用微生物生产目标产物（蛋白质）的方法。当使用该微生物时不形成孢子，从而能够减少能量损失，副产物的产生和特定的生产速度，从而能够生产目标产物（蛋白质），从而减少不必要的培养基消耗。此外，可以延长生产周期，由此有效地生产目标产物（蛋白质）。

7. 发明申请

US20080014608A1 Modified Promoter 失效
标题翻译：修饰的启动子
公开(公告)号：US20080014608A1
公开(公告)日：2008-01-17
申请号：US10589960
申请日：2005-03-04
申请人： Keiji Endo , Katsuya Ozaki
发明人： Keiji Endo , Katsuya Ozaki
IPC分类号： C12P21/04 , C07H21/04 , C12N1/20 , C12N15/00 , C12N15/11
CPC分类号： C12N15/75 , C07K14/32 , C12N9/2408 , C12N9/2411 , C12N9/2417 , C12N9/2437
摘要： The present invention provides a modified promoter DNA capable of enhancing transcription of genes encoding proteins or polypeptides, and a method for producing proteins or polypeptides efficiently by use of the modified promoter DNA.A promoter DNA recognized by SigA and SigE, which is produced by modifying a nucleotide sequence including a promoter recognized by SigA and bases in the vicinity thereof; an expression vector harboring the promoter DNA; a recombinant microorganism containing the expression vector; and a method for producing proteins or polypeptides characterized by culturing the recombinant microorganism.
摘要翻译：本发明提供能够增强编码蛋白质或多肽的基因的转录的修饰的启动子DNA，以及通过使用经修饰的启动子DNA有效产生蛋白质或多肽的方法。由SigA和SigE鉴定的启动子DNA，其通过修饰包括SigA识别的启动子和其附近的碱基的核苷酸序列而产生; 携带启动子DNA的表达载体; 含有表达载体的重组微生物; 以及产生以培养重组微生物为特征的蛋白质或多肽的方法。

8. 发明申请

US20050181493A1 Mutant alpha-amylases 审中-公开
标题翻译：突变型α-淀粉酶
公开(公告)号：US20050181493A1
公开(公告)日：2005-08-18
申请号：US10988529
申请日：2004-11-16
申请人： Keiji Endo , Kazuaki Igarashi , Yasuhiro Hayashi , Hiroshi Hagihara , Katsuya Ozaki
发明人： Keiji Endo , Kazuaki Igarashi , Yasuhiro Hayashi , Hiroshi Hagihara , Katsuya Ozaki
IPC分类号： C11D3/386 , C12N1/21 , C12N9/28 , C12N15/56 , C11D7/42
CPC分类号： C12N9/2417 , C11D3/386
摘要： The invention relates to a mutant α-amylase obtained by making replacement or deletion of at least one of amino acid residues such as the 167th Gln, 169th Tyr and 178th Ala in the amino acid sequence set forth in SEQ ID NO:1 in an α-amylase having said amino acid sequence, or an α-amylase having a homology of at least 70% to said amino acid sequence, a gene encoding the mutant α-amylase, a vector, transformed cells, a process for producing a mutant α-amylase, comprising culturing the transformed cells, and a detergent composition comprising the mutant α-amylase. The mutant α-amylase of the invention has excellent properties of high resistance to chelating agents, high specific activity in an alkaline region and excellent stability to heat, and is hence useful for detergents for automatic dish washer, laundry detergents and the like.
摘要翻译：本发明涉及通过使SEQ ID NO：1所示的氨基酸序列中的至少一个氨基酸残基例如第167位Gln，第169位和第178位氨基酸置换成α位点而获得的突变型α-淀粉酶具有所述氨基酸序列的淀粉酶或与所述氨基酸序列具有至少70％同源性的α-淀粉酶，编码突变型α-淀粉酶的基因，载体，转化细胞，用于产生突变型α-淀粉酶的方法，包括培养转化细胞的淀粉酶和包含突变型α-淀粉酶的洗涤剂组合物。本发明的突变型α-淀粉酶具有优异的抗螯合剂抗性，在碱性区域中具有高比活性和优异的耐热稳定性，因此可用于自动洗碗机，衣物洗涤剂等的洗涤剂。

9. 发明授权

US08623631B2 Modified promoter 失效
标题翻译：修饰的启动子
公开(公告)号：US08623631B2
公开(公告)日：2014-01-07
申请号：US10589960
申请日：2005-03-04
申请人： Keiji Endo , Katsuya Ozaki
发明人： Keiji Endo , Katsuya Ozaki
IPC分类号： C12N1/20 , C12P21/00 , C07H21/04
CPC分类号： C12N15/75 , C07K14/32 , C12N9/2408 , C12N9/2411 , C12N9/2417 , C12N9/2437
摘要： The present invention provides a modified promoter DNA capable of enhancing transcription of genes encoding proteins or polypeptides, and a method for producing proteins or polypeptides efficiently by use of the modified promoter DNA.A promoter DNA recognized by SigA and SigE, which is produced by modifying a nucleotide sequence including a promoter recognized by SigA and bases in the vicinity thereof; an expression vector harboring the promoter DNA; a recombinant microorganism containing the expression vector; and a method for producing proteins or polypeptides characterized by culturing the recombinant microorganism.
摘要翻译：本发明提供能够增强编码蛋白质或多肽的基因的转录的修饰的启动子DNA，以及通过使用经修饰的启动子DNA有效产生蛋白质或多肽的方法。由SigA和SigE鉴定的启动子DNA，其通过修饰包括SigA识别的启动子和其附近的碱基的核苷酸序列而产生; 携带启动子DNA的表达载体; 含有表达载体的重组微生物; 以及产生以培养重组微生物为特征的蛋白质或多肽的方法。

10. 发明申请

US20090226961A1 MUTANT ALPHA-AMYLASES 审中-公开
标题翻译：突变型ALPHA-AMYLASES
公开(公告)号：US20090226961A1
公开(公告)日：2009-09-10
申请号：US11864245
申请日：2007-09-28
申请人： Keiji Endo , Kazuaki Igarashi , Yasuhiro Hayashi , Hiroshi Hagihara , Katsuya Ozaki
发明人： Keiji Endo , Kazuaki Igarashi , Yasuhiro Hayashi , Hiroshi Hagihara , Katsuya Ozaki
IPC分类号： C12P21/00 , C07H21/00 , C12N15/63 , C12N1/00
CPC分类号： C12N9/2417 , C11D3/386
摘要： The invention relates to a mutant α-amylase obtained by making replacement or deletion of at least one of amino acid residues such as the 167th Gln, 169th Tyr and 178th Ala in the amino acid sequence set forth in SEQ ID NO:1 in an α-amylase having said amino acid sequence, or an α-amylase having a homology of at least 70% to said amino acid sequence, a gene encoding the mutant α-amylase, a vector, transformed cells, a process for producing a mutant α-amylase, comprising culturing the transformed cells, and a detergent composition comprising the mutant α-amylase.The mutant α-amylase of the invention has excellent properties of high resistance to chelating agents, high specific activity in an alkaline region and excellent stability to heat, and is hence useful for detergents for automatic dish washer, laundry detergents and the like.
摘要翻译：本发明涉及通过使SEQ ID NO：1所示的氨基酸序列中的至少一个氨基酸残基例如第167位Gln，第169位和第178位氨基酸置换成α位点而获得的突变型α-淀粉酶具有所述氨基酸序列的淀粉酶，或与所述氨基酸序列具有至少70％同源性的α-淀粉酶，编码突变型α-淀粉酶的基因，载体，转化细胞，产生突变型α-淀粉酶的方法，包括培养转化细胞的淀粉酶和包含突变型α-淀粉酶的洗涤剂组合物。本发明的突变型α-淀粉酶具有优异的抗螯合剂抗性，在碱性区域中具有高比活性和优异的耐热稳定性，因此可用于自动洗碗机，衣物洗涤剂等的洗涤剂。

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