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1. 发明申请

US20050181493A1 Mutant alpha-amylases 审中-公开
标题翻译：突变型α-淀粉酶
公开(公告)号：US20050181493A1
公开(公告)日：2005-08-18
申请号：US10988529
申请日：2004-11-16
申请人： Keiji Endo , Kazuaki Igarashi , Yasuhiro Hayashi , Hiroshi Hagihara , Katsuya Ozaki
发明人： Keiji Endo , Kazuaki Igarashi , Yasuhiro Hayashi , Hiroshi Hagihara , Katsuya Ozaki
IPC分类号： C11D3/386 , C12N1/21 , C12N9/28 , C12N15/56 , C11D7/42
CPC分类号： C12N9/2417 , C11D3/386
摘要： The invention relates to a mutant α-amylase obtained by making replacement or deletion of at least one of amino acid residues such as the 167th Gln, 169th Tyr and 178th Ala in the amino acid sequence set forth in SEQ ID NO:1 in an α-amylase having said amino acid sequence, or an α-amylase having a homology of at least 70% to said amino acid sequence, a gene encoding the mutant α-amylase, a vector, transformed cells, a process for producing a mutant α-amylase, comprising culturing the transformed cells, and a detergent composition comprising the mutant α-amylase. The mutant α-amylase of the invention has excellent properties of high resistance to chelating agents, high specific activity in an alkaline region and excellent stability to heat, and is hence useful for detergents for automatic dish washer, laundry detergents and the like.
摘要翻译：本发明涉及通过使SEQ ID NO：1所示的氨基酸序列中的至少一个氨基酸残基例如第167位Gln，第169位和第178位氨基酸置换成α位点而获得的突变型α-淀粉酶具有所述氨基酸序列的淀粉酶或与所述氨基酸序列具有至少70％同源性的α-淀粉酶，编码突变型α-淀粉酶的基因，载体，转化细胞，用于产生突变型α-淀粉酶的方法，包括培养转化细胞的淀粉酶和包含突变型α-淀粉酶的洗涤剂组合物。本发明的突变型α-淀粉酶具有优异的抗螯合剂抗性，在碱性区域中具有高比活性和优异的耐热稳定性，因此可用于自动洗碗机，衣物洗涤剂等的洗涤剂。

2. 发明申请

US20080026976A1 Highly productive alpha-amylases 审中-公开
标题翻译：高生产力的α-淀粉酶
公开(公告)号：US20080026976A1
公开(公告)日：2008-01-31
申请号：US11590769
申请日：2006-11-01
申请人： Hiroyuki Araki , Keiji Endo , Hiroshi Hagihara , Kazuaki Igarashi , Yasuhiro Hayashi , Katsuya Ozaki
发明人： Hiroyuki Araki , Keiji Endo , Hiroshi Hagihara , Kazuaki Igarashi , Yasuhiro Hayashi , Katsuya Ozaki
IPC分类号： C11D3/386 , C07H21/00 , C12N1/00 , C12N15/63 , C12N9/26 , C12P21/00
CPC分类号： C12N9/2417 , C11D3/386
摘要： The invention relates to mutant α-amylases that may be produced at high yield from recombinant microorganisms.
摘要翻译：本发明涉及可以从重组微生物以高产率产生的突变型α-淀粉酶。

3. 发明授权

US07078212B1 Mutant α-amylases 失效
标题翻译：突变型α-淀粉酶
公开(公告)号：US07078212B1
公开(公告)日：2006-07-18
申请号：US09590375
申请日：2000-06-09
申请人： Keiji Endo , Kazuaki Igarashi , Yasuhiro Hayashi , Hiroshi Hagihara , Katsuya Ozaki
发明人： Keiji Endo , Kazuaki Igarashi , Yasuhiro Hayashi , Hiroshi Hagihara , Katsuya Ozaki
IPC分类号： C12N9/26 , C12N9/28 , C07H21/04
CPC分类号： C12N9/2417 , C11D3/386
摘要： The invention relates to a mutant α-amylase obtained by making replacement or deletion of at least one of amino acid residues such as the 167th Gln, 169th Tyr and 178th Ala in the amino acid sequence set forth in SEQ ID NO:1 in an α-amylase having said amino acid sequence, or an α-amylase having a homology of at least 70% to said amino acid sequence, a gene encoding the mutant α-amylase, a vector, transformed cells, a process for producing a mutant α-amylase, comprising culturing the transformed cells, and a detergent composition comprising the mutant α-amylase.The mutant α-amylase of the invention has excellent properties of high resistance to chelating agents, high specific activity in an alkaline region and excellent stability to heat, and is hence useful for detergents for automatic dish washer, laundry detergents and the like.
摘要翻译：本发明涉及通过使SEQ ID NO：1所示的氨基酸序列中的至少一个氨基酸残基例如第167位Gln，第169位和第178位氨基酸置换成α位点而获得的突变型α-淀粉酶具有所述氨基酸序列的淀粉酶或与所述氨基酸序列具有至少70％同源性的α-淀粉酶，编码突变型α-淀粉酶的基因，载体，转化细胞，用于产生突变型α-淀粉酶的方法，包括培养转化细胞的淀粉酶和包含突变型α-淀粉酶的洗涤剂组合物。本发明的突变型α-淀粉酶具有优异的抗螯合剂抗性，在碱性区域中具有高比活性和优异的耐热稳定性，因此可用于自动洗碗机，衣物洗涤剂等的洗涤剂。

4. 发明授权

US06916645B2 Amylases 失效
标题翻译：淀粉酶
公开(公告)号：US06916645B2
公开(公告)日：2005-07-12
申请号：US10136272
申请日：2002-05-02
申请人： Hiroshi Hagihara , Kaori Kitayama , Yasuhiro Hayashi , Kazuaki Igarashi , Keiji Endo , Katsuya Ozaki
发明人： Hiroshi Hagihara , Kaori Kitayama , Yasuhiro Hayashi , Kazuaki Igarashi , Keiji Endo , Katsuya Ozaki
IPC分类号： C12N9/28 , C12N15/31 , C12N15/56 , C12N15/09 , C07H21/04 , C12N1/20 , C12N9/00
CPC分类号： C12N9/2417
摘要： Liquefying alkaline amylases, each having residual activity not less than 70% when treated at pH 10 and 45° C. for 30 minutes in the presence of 1 to 100 mM of EDTA or EGTA, are described. Also described are detergents comprising these amylases. In comparison with conventional amylases for detergents, the liquefying alkaline amylases of this invention have a high chelating-agent resisting performance.
摘要翻译：描述了在1至100mM EDTA或EGTA的存在下，在pH10和45℃下处理30分钟时，残留活性不低于70％的液体碱性淀粉酶。还描述了包含这些淀粉酶的洗涤剂。与用于洗涤剂的常规淀粉酶相比，本发明的液化碱性淀粉酶具有高螯合剂抵抗性能。

5. 发明授权

US06403355B1 Amylases 有权
标题翻译：淀粉酶
公开(公告)号：US06403355B1
公开(公告)日：2002-06-11
申请号：US09465519
申请日：1999-12-16
申请人： Hiroshi Hagihara , Kaori Kitayama , Yasuhiro Hayashi , Kazuaki Igarashi , Keiji Endo , Katsuya Ozaki
发明人： Hiroshi Hagihara , Kaori Kitayama , Yasuhiro Hayashi , Kazuaki Igarashi , Keiji Endo , Katsuya Ozaki
IPC分类号： C12N900
CPC分类号： C12N9/2417
摘要： Described are liquefying alkaline amylases each having residual activity not less than 70% when treated at pH 10 and 45° C. for 30 minutes in the presence of 1 to 100 mM of EDTA or EGTA; and a detergent comprising the same. Compared with the conventional amylases for a detergent, they have high chelating-agent resisting performance.
摘要翻译：描述了在1至100mM EDTA或EGTA的存在下，在pH 10和45℃下处理30分钟时液化碱性淀粉酶，其各自具有不小于70％的残留活性; 和含有该洗涤剂的洗涤剂。与用于洗涤剂的常规淀粉酶相比，它们具有高螯合剂抗性。

6. 发明申请

US20090226961A1 MUTANT ALPHA-AMYLASES 审中-公开
标题翻译：突变型ALPHA-AMYLASES
公开(公告)号：US20090226961A1
公开(公告)日：2009-09-10
申请号：US11864245
申请日：2007-09-28
申请人： Keiji Endo , Kazuaki Igarashi , Yasuhiro Hayashi , Hiroshi Hagihara , Katsuya Ozaki
发明人： Keiji Endo , Kazuaki Igarashi , Yasuhiro Hayashi , Hiroshi Hagihara , Katsuya Ozaki
IPC分类号： C12P21/00 , C07H21/00 , C12N15/63 , C12N1/00
CPC分类号： C12N9/2417 , C11D3/386
摘要： The invention relates to a mutant α-amylase obtained by making replacement or deletion of at least one of amino acid residues such as the 167th Gln, 169th Tyr and 178th Ala in the amino acid sequence set forth in SEQ ID NO:1 in an α-amylase having said amino acid sequence, or an α-amylase having a homology of at least 70% to said amino acid sequence, a gene encoding the mutant α-amylase, a vector, transformed cells, a process for producing a mutant α-amylase, comprising culturing the transformed cells, and a detergent composition comprising the mutant α-amylase.The mutant α-amylase of the invention has excellent properties of high resistance to chelating agents, high specific activity in an alkaline region and excellent stability to heat, and is hence useful for detergents for automatic dish washer, laundry detergents and the like.
摘要翻译：本发明涉及通过使SEQ ID NO：1所示的氨基酸序列中的至少一个氨基酸残基例如第167位Gln，第169位和第178位氨基酸置换成α位点而获得的突变型α-淀粉酶具有所述氨基酸序列的淀粉酶，或与所述氨基酸序列具有至少70％同源性的α-淀粉酶，编码突变型α-淀粉酶的基因，载体，转化细胞，产生突变型α-淀粉酶的方法，包括培养转化细胞的淀粉酶和包含突变型α-淀粉酶的洗涤剂组合物。本发明的突变型α-淀粉酶具有优异的抗螯合剂抗性，在碱性区域中具有高比活性和优异的耐热稳定性，因此可用于自动洗碗机，衣物洗涤剂等的洗涤剂。

7. 发明授权

US07297527B2 Highly productive α-amylases 失效
标题翻译：高生产力的α-淀粉酶
公开(公告)号：US07297527B2
公开(公告)日：2007-11-20
申请号：US10798278
申请日：2004-03-12
申请人： Hiroyuki Araki , Keiji Endo , Hiroshi Hagihara , Kazuaki Igarashi , Yasuhiro Hayashi , Katsuya Ozaki
发明人： Hiroyuki Araki , Keiji Endo , Hiroshi Hagihara , Kazuaki Igarashi , Yasuhiro Hayashi , Katsuya Ozaki
IPC分类号： C12N9/00 , C12N9/28 , C11D3/00 , C11D7/42
CPC分类号： C12N9/2417 , C11D3/386
摘要： The invention relates to mutant α-amylases that may be produced at high yield from recombinant microorganisms.
摘要翻译：本发明涉及可以从重组微生物以高产率产生的突变型α-淀粉酶。

8. 发明授权

US06743616B2 Highly productive alpha-amylases 失效
标题翻译：高生产力的α-淀粉酶
公开(公告)号：US06743616B2
公开(公告)日：2004-06-01
申请号：US09971611
申请日：2001-10-09
申请人： Hiroyuki Araki , Keiji Endo , Hiroshi Hagihara , Kazuaki Igarashi , Yasuhiro Hayashi , Katsuya Ozaki
发明人： Hiroyuki Araki , Keiji Endo , Hiroshi Hagihara , Kazuaki Igarashi , Yasuhiro Hayashi , Katsuya Ozaki
IPC分类号： C12N900
CPC分类号： C12N9/2417 , C11D3/386
摘要： The invention relates to mutant &agr;-amylases that may be produced at high yield from recombinant microorganisms.
摘要翻译：本发明涉及可以从重组微生物以高产率产生的突变型α-淀粉酶。

9. 发明授权

US06486113B1 Mutant &agr;-amylases 失效
标题翻译：突变型α-淀粉酶
公开(公告)号：US06486113B1
公开(公告)日：2002-11-26
申请号：US09381687
申请日：1999-09-23
申请人： Yuji Hatada , Kaori Ikawa , Susumu Ito , Kazuaki Igarashi , Hiroshi Hagihara , Yasuhiro Hayashi , Hiroyuki Araki , Katsuya Ozaki
发明人： Yuji Hatada , Kaori Ikawa , Susumu Ito , Kazuaki Igarashi , Hiroshi Hagihara , Yasuhiro Hayashi , Hiroyuki Araki , Katsuya Ozaki
IPC分类号： C11D3386
CPC分类号： C12N9/2417 , C11D3/386
摘要： The invention relates to a mutant &agr;-amylase having an amino acid sequence obtained by making deletion or replacement by another arbitrary amino acid residue of at least a methionine residue at the 202-position or a position homologous thereto among amino acid residues set forth in SEQ ID NO:1, which constitute a liquefying alkaline &agr;-amylase, a gene thereof, and a detergent composition comprising the mutant &agr;-amylase. The mutant &agr;-amylase has the optimum pH in an alkaline range, an excellent &agr;-amylase activity, and high and lasting resistance to oxidizing agents, and is hence particularly useful as a component of detergent compositions containing a bleaching agent and an oxidizing agent.
摘要翻译：本发明涉及一种具有氨基酸序列的突变型α-淀粉酶，该氨基酸序列是通过在SEQ ID NO：1所示的氨基酸残基之间至少含有202位的甲硫氨酸残基或其同源位置的另外任意氨基酸残基进行缺失或替换而获得的 ID NO：1，其构成液化碱性α-淀粉酶，其基因和包含突变型α-淀粉酶的洗涤剂组合物。突变型α-淀粉酶在碱性范围内具有最佳pH，优异的α-淀粉酶活性和对氧化剂的高持久耐受性，因此特别可用作含有漂白剂和氧化剂的洗涤剂组合物的组分。

10. 发明授权

US08084237B2 Microorganism and method for producing dodecahydro-3a,6,6,9a-tetramethylnaphtho[2,1-b]furan intermediate using the novel microorganism 有权
标题翻译：微生物和使用新型微生物制备十二氢-3a,6,6,9a-四甲基萘并[2,1-b]呋喃中间体的方法
公开(公告)号：US08084237B2
公开(公告)日：2011-12-27
申请号：US12280067
申请日：2006-12-21
申请人： Kazuaki Igarashi , Shuichi Takizawa , Norihiko Higaki , Hiroshi Hagihara
发明人： Kazuaki Igarashi , Shuichi Takizawa , Norihiko Higaki , Hiroshi Hagihara
IPC分类号： C12P17/04 , C12N1/16 , C12N1/14
CPC分类号： C12N1/14 , C12P17/04
摘要： This invention relates to a novel microorganism that efficiently produces a dodecahydro-3a,6,6,9a-tetramethylnaphtho[2,1-b]furan intermediate using sclareol as a substrate. As a result of concentrated studies, a plurality of novel microorganisms having properties of interest that are not classified as conventional microorganisms were isolated and identified. The novel microorganism of the present invention belongs to Ascomycetes and has the ability of producing a dodecahydro-3a,6,6,9a-tetramethylnaphtho[2,1-b]furan intermediate using sclareol as a substrate. Such microorganism of Ascomycetes represents a new finding and it can be effective for producing dodecahydro-3a,6,6,9a-tetramethylnaphtho[2,1-b]furan and an intermediate thereof.
摘要翻译：本发明涉及一种利用香紫苏醇作为底物有效生产十二氢-3a,6,6,9a-四甲基萘并[2,1-b]呋喃中间体的新型微生物。作为浓缩研究的结果，分离并鉴定出许多具有未分类为常规微生物的感兴趣的新型微生物。本发明的新型微生物属于子囊菌纲，具有使用香紫苏醇作为底物生产十二氢-3a,6,6,9a-四甲基萘并[2,1-b]呋喃中间体的能力。子囊菌的这种微生物代表新的发现，对于生产十二氢-3a,6,6,9a-四甲基萘并[2,1-b]呋喃及其中间体是有效的。

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