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1. 发明申请

US20090081726A1 Recombinant Microorganism 有权
标题翻译：重组微生物
公开(公告)号：US20090081726A1
公开(公告)日：2009-03-26
申请号：US11722162
申请日：2005-12-20
申请人： Takeko Kodama , Keiji Endo , Katsuya Ozaki , Junichi Sekiguchi
发明人： Takeko Kodama , Keiji Endo , Katsuya Ozaki , Junichi Sekiguchi
IPC分类号： C12P21/04 , C12N1/21
CPC分类号： C12P21/02 , C07K14/32 , C12N9/54 , C12N15/75
摘要： A host microorganism capable of increasing productivity of a protein or polypeptide, a recombinant microorganism obtained by introducing a gene encoding a protein or polypeptide into the host microorganism, and a method for producing a protein or polypeptide using the recombinant microorganism are provided.Also provided is a recombinant microorganism obtained by introducing into a host microorganism a gene encoding a heterologous protein or polypeptide, wherein in said host microorganism the Bacillus subtilis aprX gene or a gene corresponding to the aprX gene has been deleted or knocked out, and a method for producing a protein or polypeptide using the recombinant microorganism.
摘要翻译：提供能够提高蛋白质或多肽的生产力的宿主微生物，通过将编码蛋白质或多肽的基因导入宿主微生物而获得的重组微生物，以及使用该重组微生物产生蛋白质或多肽的方法。还提供了通过向宿主微生物中导入编码异源蛋白质或多肽的基因而获得的重组微生物，其中在所述宿主微生物中，枯草芽孢杆菌aprX基因或与aprX基因相对应的基因已被缺失或敲除，用于使用重组微生物产生蛋白质或多肽。

2. 发明授权

US06689874B2 Gene for enzyme having both alkaline pullulanase and alkaline alpha-amylase activities 失效
标题翻译：具有碱性支链淀粉酶和碱性α-淀粉酶活性的酶的基因
公开(公告)号：US06689874B2
公开(公告)日：2004-02-10
申请号：US10014436
申请日：2001-12-14
申请人： Yuji Hatada , Kazuaki Igarashi , Katsuya Ozaki , Katsutoshi Ara , Shuji Kawai , Susumu Ito
发明人： Yuji Hatada , Kazuaki Igarashi , Katsuya Ozaki , Katsutoshi Ara , Shuji Kawai , Susumu Ito
IPC分类号： C07H2104
CPC分类号： C12N9/2457 , C12N9/2417 , C12N9/2451 , C12Y302/01001 , C12Y302/01041
摘要： The present invention provides: a nucleotide sequence encoding alkaline pullulanase and exhibiting both alkaline &agr;-amylase and alkaline pullulanase activity; a nucleotide sequence encoding alkaline &agr;-amylase possessing an amino acid sequence described in SEQ ID NO:3; a nucleotide sequence encoding alkaline pullulanase possessing an amino acid sequence described in SEQ ID NO:4; recombinant DNAs containing these nucleotide sequences, and transformed microorganisms harboring the recombinant DNAs. The technique of the present invention enables mass production of alkaline pullulanase exhibiting both alkaline &agr;-amylase and alkaline pullulanase activity.
摘要翻译：本发明提供：编码碱基支链淀粉酶并表现出碱性α-淀粉酶和碱性支链淀粉酶活性的核苷酸序列; 编码具有SEQ ID NO：3所述的氨基酸序列的碱性α-淀粉酶的核苷酸序列; 编码具有SEQ ID NO：4所述的氨基酸序列的碱基支链淀粉酶的核苷酸序列; 含有这些核苷酸序列的重组DNA和携带重组DNA的转化微生物。本发明的技术能够大量生产表现出碱性α-淀粉酶和碱性支链淀粉酶活性的碱性支链淀粉酶。

3. 发明授权

US5045464A Alkaline cellulase and process for producing the same 失效
标题翻译：碱性纤维素酶及其制备方法
公开(公告)号：US5045464A
公开(公告)日：1991-09-03
申请号：US339592
申请日：1989-04-14
申请人： Susumu Ito , Shuji Kawai , Shitsuw Shikata , Katsuya Ozaki , Tadashi Yoshimatsu
发明人： Susumu Ito , Shuji Kawai , Shitsuw Shikata , Katsuya Ozaki , Tadashi Yoshimatsu
IPC分类号： C11D3/386 , C12N9/42 , C12R1/07
CPC分类号： C11D3/38645 , C12N9/2445 , C12Y302/01004 , C12Y302/01021
摘要： A novel alkaline cellulase is disclosed. The alkaline cellulase is produced by Bacillus sp KSM-635 (FERM BP-1485) and possesses: (1) C.sub.x enzyme activity to act on carboxymethyl cellulose and weak C.sub.1 enzyme .beta.-glucosidase activities, (2) substrate specificity to act on carboxymethyl cellulose, cellulose cotton, Avicel, filter paper, cellobiose, and p-nitrophenyl cellobioside, (3) a working pH range of 4-12 and an optimum pH range of 9-10, (4) stable pH range of 4.5-10.5 when allowed to stand at 40.degree. C. for 10 minutes, and (5) working temperature range of 10.degree.-65.degree. C. and optimum temperature range at around 40.degree. C. Its activity is not inhibited by chelating agents nor by surface active agents. It is particularly effective as an additive for laundry detergent compositions.

4. 发明授权

US07829322B2 Recombinant microorganism comprising inactivation of the AprX gene 有权
标题翻译：包含AprX基因失活的重组微生物
公开(公告)号：US07829322B2
公开(公告)日：2010-11-09
申请号：US11722162
申请日：2005-12-20
申请人： Takeko Kodama , Keiji Endo , Katsuya Ozaki , Junichi Sekiguchi
发明人： Takeko Kodama , Keiji Endo , Katsuya Ozaki , Junichi Sekiguchi
IPC分类号： C12N1/21 , C12N15/00 , C12N15/57 , C12N15/75
CPC分类号： C12P21/02 , C07K14/32 , C12N9/54 , C12N15/75
摘要： A host microorganism capable of increasing productivity of a protein or polypeptide, a recombinant microorganism obtained by introducing a gene encoding a protein or polypeptide into the host microorganism, and a method for producing a protein or polypeptide using the recombinant microorganism are provided.Also provided is a recombinant microorganism obtained by introducing into a host microorganism a gene encoding a heterologous protein or polypeptide, wherein in said host microorganism the Bacillus subtilis aprX gene or a gene corresponding to the aprX gene has been deleted or knocked out, and a method for producing a protein or polypeptide using the recombinant microorganism.
摘要翻译：提供能够提高蛋白质或多肽的生产力的宿主微生物，通过将编码蛋白质或多肽的基因导入宿主微生物而获得的重组微生物，以及使用该重组微生物产生蛋白质或多肽的方法。还提供了通过向宿主微生物中导入编码异源蛋白质或多肽的基因而获得的重组微生物，其中在所述宿主微生物中，枯草芽孢杆菌aprX基因或与aprX基因相对应的基因已被缺失或敲除，用于使用重组微生物产生蛋白质或多肽。

5. 发明申请

US20090170154A1 Mutant bacterium belonging to the genus bacillus 有权
标题翻译：属于杆菌属的突变细菌
公开(公告)号：US20090170154A1
公开(公告)日：2009-07-02
申请号：US10590275
申请日：2005-03-04
申请人： Keiji Endo , Katsuya Ozaki
发明人： Keiji Endo , Katsuya Ozaki
IPC分类号： C12P21/00 , C12N1/21 , C12N15/87
CPC分类号： C12N9/2417 , C12N9/2437 , C12N9/54 , C12Y302/01004
摘要： The present invention provides a mutant Bacillus bacterium capable of enhancing productivity of proteins or polypeptides, a recombinant microorganism produced by introducing genes encoding heterologous proteins or polypeptides into the mutant Bacillus bacterium, and a method for producing proteins or polypeptides by use of the recombinant microorganism.A mutant Bacillus bacterium which has, on the genome or plasmid thereof, DNA having a promoter sequence which is recognized and transcribed specifically during the sporulation stage and which is ligated to an upstream end of a sigA gene or a gene equivalent thereto, a recombinant microorganism produced by introducing genes encoding heterologous proteins or polypeptides into the mutant Bacillus bacterium, and a method for producing proteins or polypeptides by use of the recombinant microorganism.
摘要翻译：本发明提供能够提高蛋白质或多肽生产力的突变芽孢杆菌属细菌，通过将编码异源蛋白质或多肽的基因导入突变芽孢杆菌属细菌产生的重组微生物，以及通过使用重组微生物产生蛋白质或多肽的方法。一种突变芽孢杆菌属细菌，其在基因组或质粒上具有在孢子形成阶段中特异性识别并转录并与sigA基因或其等同基因相连的基因的上游端的启动子序列的DNA，重组微生物通过将编码异源蛋白质或多肽的基因导入突变芽孢杆菌属细菌而产生，以及通过使用重组微生物产生蛋白质或多肽的方法。

6. 发明申请

US20080081352A1 Recombinant Microorganism 失效
标题翻译：重组微生物
公开(公告)号：US20080081352A1
公开(公告)日：2008-04-03
申请号：US10578615
申请日：2004-11-05
申请人： Masatoshi Tohata , Kazuhisa Sawada , Katsuya Ozaki , Junichi Sekiguchi
发明人： Masatoshi Tohata , Kazuhisa Sawada , Katsuya Ozaki , Junichi Sekiguchi
IPC分类号： C12P21/04 , C12N1/20
CPC分类号： C12N9/2437 , C12N15/75 , C12P21/00 , C12Y302/01004
摘要： The present invention provides a recombinant microorganism obtained by transferring, into a host microorganism which is capable of producing protein or polypeptide with increased productivity and which was identified by the present inventors, a gene encoding a protein or polypeptide, and a method for producing a protein or polypeptide by use of the recombinant microorganism. The recombinant microorganism is prepared by transferring, to a mutant strain of microorganism from which any of at least one gene participating in membrane permeation of maltose has been deleted or knocked out, a gene encoding a heterologous protein or polypeptide.
摘要翻译：本发明提供了一种重组微生物，其通过将能够产生蛋白质或多肽的生物能力提高的宿主微生物转移到本发明人鉴定出的编码蛋白质或多肽的基因以及产生蛋白质的方法中或多肽。将重组微生物通过转移到至少一种参与麦芽糖的膜渗透的基因中的任何一种已被缺失或敲除的微生物的突变菌株，编码异源蛋白质或多肽的基因。

7. 发明申请

US20080050807A1 Gene encoding alkaline liquefying alpha-amylase 审中-公开
标题翻译：基因编码碱液化α-淀粉酶
公开(公告)号：US20080050807A1
公开(公告)日：2008-02-28
申请号：US10829331
申请日：2004-04-22
申请人： Yuji Hatada , Katsuya Ozaki , Katsutoshi Ara , Shuji Kawai , Susumu Ito
发明人： Yuji Hatada , Katsuya Ozaki , Katsutoshi Ara , Shuji Kawai , Susumu Ito
IPC分类号： C12N15/00 , C07H21/04
CPC分类号： C12N9/2417
摘要： The present invention provides a DNA fragment encoding alkaline liquefying α-amylase, recombinant DNA containing the DNA fragment, a transformed microorganism harboring the recombinant DNA, as well as a method for producing alkaline liquefying α-amylase using the transformant. The method of the present invention enables mass production of alkaline liquefying α-amylase useful as a detergent component.The present invention is directed to a liquefying alkaline alphα-amylase, and a DNA encoding for the same and functional fragments thereof.
摘要翻译：本发明提供了编码碱液化α-淀粉酶的DNA片段，含有DNA片段的重组DNA，携带重组DNA的转化微生物以及使用该转化体产生碱液化α-淀粉酶的方法。本发明的方法能够大量生产用作洗涤剂组分的碱性液化α-淀粉酶。本发明涉及液化碱性α-淀粉酶和编码其相同功能片段的DNA。

8. 发明申请

US20080026976A1 Highly productive alpha-amylases 审中-公开
标题翻译：高生产力的α-淀粉酶
公开(公告)号：US20080026976A1
公开(公告)日：2008-01-31
申请号：US11590769
申请日：2006-11-01
申请人： Hiroyuki Araki , Keiji Endo , Hiroshi Hagihara , Kazuaki Igarashi , Yasuhiro Hayashi , Katsuya Ozaki
发明人： Hiroyuki Araki , Keiji Endo , Hiroshi Hagihara , Kazuaki Igarashi , Yasuhiro Hayashi , Katsuya Ozaki
IPC分类号： C11D3/386 , C07H21/00 , C12N1/00 , C12N15/63 , C12N9/26 , C12P21/00
CPC分类号： C12N9/2417 , C11D3/386
摘要： The invention relates to mutant α-amylases that may be produced at high yield from recombinant microorganisms.
摘要翻译：本发明涉及可以从重组微生物以高产率产生的突变型α-淀粉酶。

9. 发明授权

US06764841B2 Production process of glucose-1-phosphate 有权
标题翻译：葡萄糖-1-磷酸的生产工艺
公开(公告)号：US06764841B2
公开(公告)日：2004-07-20
申请号：US10053550
申请日：2002-01-24
申请人： Kazuaki Igarashi , Katsuya Ozaki
发明人： Kazuaki Igarashi , Katsuya Ozaki
IPC分类号： C12P1902
CPC分类号： C12R1/15 , C12P19/02
摘要： The invention relates to a process for producing glucose-1-phosphate, which contains the steps of culturing bacteria of the genus Corynebacterium in a medium containing a saccharide and at least 1 mM of phosphoric acid or a derivative or salt thereof, and collecting glucose-1-phosphate produced and accumulated in the medium. A mass of G-1-P can be provided without conducting complicated steps which are required of the enzymatic process.
摘要翻译：本发明涉及生产葡萄糖-1-磷酸的方法，其包括在含有糖和至少1mM磷酸或其衍生物或盐的培养基中培养棒杆菌属细菌的步骤，并收集葡萄糖 - 在培养基中产生和积累的1-磷酸酯。可以提供G-1-P的质量，而不需要进行酶法处理所需的复杂步骤。

10. 发明授权

US08623631B2 Modified promoter 失效
标题翻译：修饰的启动子
公开(公告)号：US08623631B2
公开(公告)日：2014-01-07
申请号：US10589960
申请日：2005-03-04
申请人： Keiji Endo , Katsuya Ozaki
发明人： Keiji Endo , Katsuya Ozaki
IPC分类号： C12N1/20 , C12P21/00 , C07H21/04
CPC分类号： C12N15/75 , C07K14/32 , C12N9/2408 , C12N9/2411 , C12N9/2417 , C12N9/2437
摘要： The present invention provides a modified promoter DNA capable of enhancing transcription of genes encoding proteins or polypeptides, and a method for producing proteins or polypeptides efficiently by use of the modified promoter DNA.A promoter DNA recognized by SigA and SigE, which is produced by modifying a nucleotide sequence including a promoter recognized by SigA and bases in the vicinity thereof; an expression vector harboring the promoter DNA; a recombinant microorganism containing the expression vector; and a method for producing proteins or polypeptides characterized by culturing the recombinant microorganism.
摘要翻译：本发明提供能够增强编码蛋白质或多肽的基因的转录的修饰的启动子DNA，以及通过使用经修饰的启动子DNA有效产生蛋白质或多肽的方法。由SigA和SigE鉴定的启动子DNA，其通过修饰包括SigA识别的启动子和其附近的碱基的核苷酸序列而产生; 携带启动子DNA的表达载体; 含有表达载体的重组微生物; 以及产生以培养重组微生物为特征的蛋白质或多肽的方法。

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