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1. 发明授权

US07223326B2 Capillary electrophoresis system 有权
标题翻译：毛细管电泳系统
公开(公告)号：US07223326B2
公开(公告)日：2007-05-29
申请号：US10609554
申请日：2003-07-01
申请人： Takashi Anazawa , Takashi Irie , Masao Kamahori
发明人： Takashi Anazawa , Takashi Irie , Masao Kamahori
IPC分类号： G01N27/453
CPC分类号： G01N27/44726
摘要： A capillary electrophoresis system includes a plurality of capillaries arranged in parallel on a vertical plane at an identical distance from each other, each of which has a sample injection end immersed in a cathode buffer 23 and pointed downward vertically to facilitate connection between the cathode buffer 23 and a sample solution. The sample elution end is connected via a pump block 11 and a connection tube 3 to an anode buffer 24. The height is made identical between the liquid levels for buffers 23 and 24 to prevent movement of the separation medium in the capillaries. The sample introduced from the sample injection end 20 moves upward vertically via electrophoresis to the sample elution end in the capillary and is passed through and detected at the fluorescence detection position 19. The effective separation length is thus decreased to 10 cm or less to attain high throughput.
摘要翻译：毛细管电泳系统包括在垂直平面上彼此平行布置的多个毛细管，每个毛细管具有浸入阴极缓冲器23中的样品注射端，并垂直向下指向以便于阴极缓冲器23之间的连接和样品溶液。样品洗脱端通过泵块11和连接管3连接到阳极缓冲器24.在缓冲器23和24的液面之间的高度相同，以防止分离介质在毛细管中移动。从样品注射端20引入的样品通过电泳向上垂直移动到毛细管中的样品洗脱端，并在荧光检测位置19通过并检测。因此有效分离长度降低到10cm或更小，以获得高产量。

2. 发明授权

US06635164B1 Capillary electrophoresis system 有权
标题翻译：毛细管电泳系统
公开(公告)号：US06635164B1
公开(公告)日：2003-10-21
申请号：US09696262
申请日：2000-10-26
申请人： Takashi Anazawa , Takashi Irie , Masao Kamahori
发明人： Takashi Anazawa , Takashi Irie , Masao Kamahori
IPC分类号： C02F140
CPC分类号： G01N27/44726
摘要： A capillary electrophoresis system includes a plurality of capillaries arranged in parallel on a vertical plane at an identical distance from each other, each of which has a sample injection end immersed in a cathode buffer 23 and pointed downward vertically to facilitate connection between the cathode buffer 23 and a sample solution. The sample elution end is connected via a pump block 11 and a connection tube 3 to an anode buffer 24. The height is made identical between the liquid levels for buffers 23 and 24 to prevent movement of the separation medium in the capillaries. The sample introduced from the sample injection end 20 moves upward vertically via electrophoresis to the sample elution end in the capillary and is passed through and detected at the fluorescence detection position 19. The effective separation length is thus decreased to 10 cm or less to attain high throughput.
摘要翻译：毛细管电泳系统包括在垂直平面上彼此平行布置的多个毛细管，每个毛细管具有浸入阴极缓冲器23中的样品注射端，并垂直向下指向以便于阴极缓冲器23之间的连接和样品溶液。样品洗脱端通过泵块11和连接管3连接到阳极缓冲器24.在缓冲器23和24的液面之间的高度相同，以防止分离介质在毛细管中移动。从样品注射端20引入的样品通过电泳向上垂直移动到毛细管中的样品洗脱端，并在荧光检测位置19处通过并检测。因此有效分离长度降低到10cm以下以达到高吞吐量。

3. 发明申请

US20130029872A1 ARRAY FOR DETECTING BIOLOGICAL SUBSTANCE, ASSAY SYSTEM AND ASSAY METHOD 审中-公开
标题翻译：用于检测生物物质，测定系统和测定方法的阵列
公开(公告)号：US20130029872A1
公开(公告)日：2013-01-31
申请号：US13639350
申请日：2011-04-04
申请人： Yu Ishige , Masao Kamahori , Yusuke Goto
发明人： Yu Ishige , Masao Kamahori , Yusuke Goto
IPC分类号： C40B40/10 , C40B30/04
CPC分类号： G01N33/569 , G01N27/3275 , G01N33/5438
摘要： Provided are a device, whereby cells, a bacterium or a virus can be quantified in a single unit, an assay system and an assay method. A subject to be assayed such as cells, a bacterium or a virus, which are present on a sensor, can be quantified by using a sensor equipped with multiple electrodes, said electrodes being similar in size to the subject to be assayed, detecting, concerning each electrode, the presence or absence of the subject in the vicinity of the electrode, and adding up the electrodes in which the subject is detected.
摘要翻译：提供了一种装置，其中细胞，细菌或病毒可以在单个单位中量化，测定系统和测定方法。可以通过使用配备有多个电极的传感器来量化存在于传感器上的诸如细胞，细菌或病毒的受试者，所述电极的尺寸与要测定的受试者相似，检测有关每个电极，在电极附近存在或不存在受试者，并且将检测到被检体的电极相加。

4. 发明授权

US08323468B2 Ion concentration measuring device and ion concentration measuring element 有权
标题翻译：离子浓度测量装置和离子浓度测量元件
公开(公告)号：US08323468B2
公开(公告)日：2012-12-04
申请号：US12081736
申请日：2008-04-21
申请人： Masao Kamahori , Yu Ishige , Kotaro Yamashita , Yasuhisa Shibata , Masafumi Miyake
发明人： Masao Kamahori , Yu Ishige , Kotaro Yamashita , Yasuhisa Shibata , Masafumi Miyake
IPC分类号： G01N27/333
CPC分类号： G01N27/414
摘要： A crown ether derivative that acts as cation capturing ligand and alkanethiol having a longer carbon chain than a linker are immobilized, coexisting together, on the surface of a gold electrode, by using as the linker an insulating molecule (e.g., alkanethiol) that forms self-assembled monolayers. Electromotive force produced in association with cation coordination is measured by a potentiometer through a change in interfacial potential on the surface of the gold electrode. Further, an insulated gate field effect transistor formed on the same substrate as the gold electrode is used as the potentiometer. Furthermore, a straight-chain polymer physically adsorbed on the gold electrode is used in order to reduce the influence of the adsorption of impurities on the surface of the electrode during biological sample measurement.
摘要翻译：作为阳离子捕获配体的冠醚衍生物和具有比接头长的碳链的链烷硫醇通过使用形成自身的绝缘分子（例如，链烷硫醇）固定在金电极的表面上并存在一起，在金电极的表面上共存组合单层。通过电极计通过金电极表面的界面电位的变化来测量与阳离子配位相关产生的电动势。此外，与金电极形成在同一衬底上的绝缘栅场效应晶体管被用作电位计。此外，使用物理吸附在金电极上的直链聚合物，以便在生物样品测量期间减少杂质在电极表面上的吸附的影响。

5. 发明授权

US08128796B2 Analyzer 有权
标题翻译：分析仪
公开(公告)号：US08128796B2
公开(公告)日：2012-03-06
申请号：US12155919
申请日：2008-06-11
申请人： Yu Ishige , Masao Kamahori
发明人： Yu Ishige , Masao Kamahori
IPC分类号： G01N33/487
CPC分类号： G01N33/492
摘要： It is an object of this invention to measure small amounts of a plurality of sample solutions at the same time. The small amounts of sample solutions are respectively placed on measuring electrodes, a medium is placed across the plurality of sample solutions, a liquid joint of a reference electrode is brought into contact with the medium, and a potential difference between each of the measuring electrodes and the reference electrode via the medium is measured.
摘要翻译：本发明的一个目的是同时测量少量的多个样品溶液。将少量样品溶液分别放置在测量电极上，将介质放置在多个样品溶液上，使参考电极的液体接头与介质接触，并且测量电极和通过介质测量参考电极。

6. 发明授权

US4823168A Flow-through cell for a photometer formed using a pair of cell body members 失效
标题翻译：用于使用一对电池体构件形成的光度计的流通池
公开(公告)号：US4823168A
公开(公告)日：1989-04-18
申请号：US116543
申请日：1987-11-04
申请人： Masao Kamahori , Yoshio Watanabe , Junkichi Miura , Mamoru Taki , Hiroyuki Miyagi
发明人： Masao Kamahori , Yoshio Watanabe , Junkichi Miura , Mamoru Taki , Hiroyuki Miyagi
IPC分类号： G01N21/05 , G01N30/74
CPC分类号： G01N21/05 , G01N2021/0346 , G01N2021/054 , G01N2030/746 , G01N30/74
摘要： A flow cell for a photometer, which comprises a cell body integrated from a pair of cell body members by joining, at least one of which is provided with a linear groove on the joining surface of the cell body member from one end to another to the full length, as exposed to the joining surface, the groove playing both roles of a liquid sample passage and a detecting light path by the integration of the cell body members, and is further provided with a liquid sample inlet passage at a position near one end of the groove and a liquid sample outlet passage at a position near the other end of the groove, both passages being communicated with the grooves, the linear groove being provided with a light reflecting layer to the full length of the linear groove, and a pair of light transmission window members joined with the flow cell body at both ends on the groove-open sides thereof is provided, where polishing of the grooves is readily carried out and random reflection of light or absorption of light due to poor reflection is reduced, and the turbulent flow of a liquid sample and generation of noise are suppressed.
摘要翻译：一种用于光度计的流动池，其包括通过接合从一对电池主体部件集成的电池体，其中至少一个在电池体部件的接合表面上从一端到另一端设置有线性槽全长，暴露于接合表面，沟槽通过集成电池主体部件而兼容液体样品通道和检测光路两者，并且在靠近一端的位置处进一步设置有液体样品入口通道的槽和在槽的另一端附近的位置处的液体样品出口通道，两个通道与槽连通，所述直线槽在直线槽的整个长度上设置有光反射层，以及一对提供了在其开槽侧的两端与流通池本体连接的透光窗构件，其中容易进行槽的抛光和光或吸收的随机反射o 由于反射不良导致的光衰减，液体试样的紊流和噪声的产生被抑制。

7. 发明授权

US08246810B2 DNA analysis method and DNA analyzer 有权
标题翻译： DNA分析方法和DNA分析仪
公开(公告)号：US08246810B2
公开(公告)日：2012-08-21
申请号：US13064682
申请日：2011-04-08
申请人： Yu Ishige , Masao Kamahori
发明人： Yu Ishige , Masao Kamahori
IPC分类号： G01N33/50
CPC分类号： C12Q1/6825 , C12Q1/6869 , C12Q2565/607 , C12Q2565/301 , C12Q2563/113
摘要： Disclosed is a DNA analysis method and a DNA analyzer whose signal intensity is not lowered even when a material at a higher density is measured. There is supplied dATP, dTTP, dGTP, or dCTP from a dATP solution vessel, a dTTP solution vessel, a dGTP solution vessel, or a dCTP solution vessel, and this causes an extension reaction of a double-stranded DNA immobilized to a bead, to yield pyrophosphoric acid. The pyrophosphoric acid is converted into a redox compound by the actions of a reagent and an enzyme contained in a reaction buffer in a reaction buffer vessel. The redox compound causes a variation in surface potential of a measuring electrode bearing an electrochemically active material immobilized thereto through an insulating molecule, and this variation causes a variation in drain current of a field-effect transistor electrically connected to the measuring electrode. Thus, the extension reaction is detected.
摘要翻译：公开了即使在测量较高密度的材料时信号强度也不降低的DNA分析方法和DNA分析仪。从dATP溶液容器，dTTP溶液容器，dGTP溶液容器或dCTP溶液容器中提供dATP，dTTP，dGTP或dCTP，这导致固定在珠粒上的双链DNA的延伸反应，得到焦磷酸。通过反应缓冲容器中的反应缓冲液中所含的试剂和酶的作用将焦磷酸转化为氧化还原化合物。氧化还原化合物导致通过绝缘分子固定有电化学活性材料的测量电极的表面电位变化，并且这种变化导致与测量电极电连接的场效应晶体管的漏极电流的变化。因此，检测到延伸反应。

8. 发明授权

US08202409B2 Potentiometric sensor and analytical element 有权
标题翻译：电位传感器和分析元件
公开(公告)号：US08202409B2
公开(公告)日：2012-06-19
申请号：US11942862
申请日：2007-11-20
申请人： Yu Ishige , Masao Kamahori
发明人： Yu Ishige , Masao Kamahori
IPC分类号： G01N27/414
CPC分类号： G01N27/4035
摘要： A potentiometric sensor with suppressed leak current on the surface of an electrode and improved for a dynamic range and a response speed, in which a redox compound is immobilized through insulative molecules on the surface of a gold electrode, and a current between a source and drain of an insulated gate field-effect transistor along with reaction between an oxidized substance or a reduced substance produced by the reaction of a measured substance in a sample solution injector for supplying the sample solution containing the measured substance and an enzyme and a redox compound on the surface of the gold electrode, is monitored on real time to measure the change of the surface potential.
摘要翻译：一种具有在电极表面上具有抑制的漏电流的电位传感器，并且改善了动态范围和响应速度，其中通过金电极表面上的绝缘分子固定氧化还原化合物，以及源极和漏极之间的电流绝缘栅场效应晶体管与通过样品溶液注射器中的测量物质的反应产生的氧化物质或还原物质之间的反应，用于将含有测量物质的样品溶液与酶和氧化还原化合物反应金电极的表面，被实时监测以测量表面电位的变化。

9. 发明申请

US20110276278A1 POTENTIOMETRIC-SENSOR CHIP, POTENTIOMETRIC ASSAY, AND ASSAY KIT 有权
标题翻译：电位传感器芯片，电位测定和测定工具包
公开(公告)号：US20110276278A1
公开(公告)日：2011-11-10
申请号：US13126766
申请日：2009-10-29
申请人： Yu Ishige , Masao Kamahori , Kuniaki Nagamine
发明人： Yu Ishige , Masao Kamahori , Kuniaki Nagamine
IPC分类号： G01N27/00 , G06F19/00
CPC分类号： G01N27/3272 , G01N27/3277 , G01N33/49 , G01N33/66
摘要： Provided is a potentiometric sensor chip in which the positional relationship among a reference electrode, a measurement electrode, and a sample inlet which enables measurement from the start of a reaction is defined, and further provided is a method for detecting the start time of the reaction. A very small amount of sample is measured with high accuracy. The very small quantity of sample is measured by a rate assay. When a reference electrode (103) is disposed between a sample inlet (102) and a measurement electrode (104), a sample solution arrives at the reference electrode (103) earlier than at the measurement electrode (104), whereby the surface potential of the measurement electrode (104) can be measured simultaneously when the sample solution arrives at the measurement electrode (104) and dissolves a reagent and thereby a reaction starts. The arrival of the sample solution at the measurement electrode (104) can be sensed by detecting the discontinuous change of the voltage observed by a voltmeter (106) at this time.
摘要翻译：提供了一种电位传感器芯片，其中定义了参考电极，测量电极和能够从反应开始进行测量的样品入口之间的位置关系，并且还提供了用于检测反应开始时间的方法。以高精度测量非常少量的样品。通过速率测定法测量非常少量的样品。当参考电极（103）设置在样品入口（102）和测量电极（104）之间时，样品溶液比测量电极（104）早到达参考电极（103），由此，当样品溶液到达测量电极（104）并溶解试剂，从而开始反应时，可以同时测量测量电极（104）。可以通过检测此时由电压表（106）观察到的电压的不连续变化来检测样品溶液到达测量电极（104）的到来。

10. 发明申请

US20060016699A1 Apparatus and method for measuring biological material 失效
公开(公告)号：US20060016699A1
公开(公告)日：2006-01-26
申请号：US11072507
申请日：2005-03-07
申请人： Masao Kamahori , Yoshiaki Yazawa , Maki Shimoda
发明人： Masao Kamahori , Yoshiaki Yazawa , Maki Shimoda
IPC分类号： G01N27/26 , G01N33/487
CPC分类号： G01N33/5438 , G01N27/4145
摘要： Disclosed is a biomolecular detection device that can be used easily, at a low operating cost, and with a detection probe that can be immobilized easily. Using an insulated gate field effect transistor having a conductive electrode on the gate insulator between a source and a drain, a probe for detecting biomolecules is immobilized on the surface of the conductive electrode. For analysis, a conductive electrode on which a probe for detecting biomolecules is immobilized on the surface, and a reference electrode are placed in the sample solution in the analytical cell, an alternating current voltage is applied from a power source to the reference electrode and the electrical characteristics of the insulated gate field effect transistor that changes before and after binding of the measurement target substance such as DNA and proteins included in the sample solution with a probe for detecting biomolecules, namely the changes in the current values running between the source and the drain, are detected.

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