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1. 发明授权

US07790109B2 Micro-fluid reaction vessel, method for manufacturing the same, and micro-fluid reaction method using the vessel 有权
标题翻译：微流体反应容器，其制造方法和使用该容器的微流体反应方法
公开(公告)号：US07790109B2
公开(公告)日：2010-09-07
申请号：US11625877
申请日：2007-01-23
申请人： Su-hyeon Kim , Jin-tae Kim , Kak Namkoong , In-ho Lee , Chin-sung Park , Young-sun Lee
发明人： Su-hyeon Kim , Jin-tae Kim , Kak Namkoong , In-ho Lee , Chin-sung Park , Young-sun Lee
IPC分类号： G01N15/06 , G01N33/00 , G01N33/48 , B01J10/00 , B01J10/02 , B01J12/00 , B01J12/02 , F15C1/04 , F15C3/00 , F15C1/06
CPC分类号： B01L3/502738 , B01L7/52 , B01L2200/0689 , B01L2300/0816 , B01L2300/0825 , B01L2300/0887 , B01L2300/123 , B01L2400/0655 , B29C33/3857 , B29C33/60 , Y10T137/218 , Y10T137/2202 , Y10T137/2224
摘要： A micro-fluid reaction vessel includes an upper plate formed of an elastomer, a lower plate adhered to the upper plate, a micro-chamber and a micro-channel formed on an inner surface of the upper plate facing the lower plate and an inlet hole and an outlet hole formed in the upper plate and through which a fluid flows into or out of, respectively. The micro-channel is constructed to be closed by pressure applied to the upper plate and elastically restored when the pressure is not applied. A micro fluid reaction method uses the micro fluid reaction vessel and a method of manufacturing forms the microfluid reaction vessel.
摘要翻译：微流体反应容器包括由弹性体形成的上板，粘附到上板的下板，形成在面向下板的上板的内表面上的微室和微通道，以及入口孔以及形成在上板中并且流体分别流入或流出的出口孔。微通道被构造为通过施加到上板的压力而关闭，并且当不施加压力时弹性恢复。微流体反应方法使用微流体反应容器和制造方法形成微流体反应容器。

2. 发明授权

US07838300B2 Method of reducing temperature difference between a pair of substrates 有权
标题翻译：降低一对基板之间的温度差的方法
公开(公告)号：US07838300B2
公开(公告)日：2010-11-23
申请号：US11689640
申请日：2007-03-22
申请人： Kak Namkoong , Su-hyeon Kim , Jin-tae Kim , Chin-sung Park , Young-sun Lee
发明人： Kak Namkoong , Su-hyeon Kim , Jin-tae Kim , Chin-sung Park , Young-sun Lee
IPC分类号： G01N25/20 , B01L3/02
CPC分类号： B81B3/0081 , B01J19/0093 , B01J2219/00783 , B01J2219/00822 , B01J2219/00873 , B01L3/5027 , B01L7/5255 , B01L2200/14 , B01L2200/147 , B01L2300/1805 , B01L2300/1827 , B81B2201/051 , F28F21/02 , F28F2013/006
摘要： A method of reducing a temperature difference between a high-temperature and a low-temperature substrate includes interposing a heat transfer facilitating layer which has a higher thermal conductivity than air and can hold particles between the substrates, and maintaining close contact between the high-temperature substrate, the heat transfer facilitating layer, and the low-temperature substrate, wherein formation of an air layer can be at least substantially prevented between the high-temperature substrate and the heat transfer facilitating layer, and between the low-temperature substrate and the heat transfer facilitating layer.
摘要翻译：降低高温和低温基板之间的温度差的方法包括：介于热传导率高于空气的传热促进层，并且可以在基板之间保持颗粒，并且保持高温基板，传热促进层和低温基板，其中至少可以在高温基板和传热促进层之间以及在低温基板和热量之间基本上防止空气层的形成转移促进层。

3. 发明授权

US08771610B2 Method of reducing temperature difference between a pair of substrates and fluid reaction device using the same 失效
标题翻译：降低一对基板与使用其的液体反应装置之间的温度差的方法
公开(公告)号：US08771610B2
公开(公告)日：2014-07-08
申请号：US12904557
申请日：2010-10-14
申请人： Kak Namkoong , Su-hyeon Kim , Jin-tae Kim , Chin-sung Park , Young-sun Lee
发明人： Kak Namkoong , Su-hyeon Kim , Jin-tae Kim , Chin-sung Park , Young-sun Lee
IPC分类号： B01L3/00
CPC分类号： B81B3/0081 , B01J19/0093 , B01J2219/00783 , B01J2219/00822 , B01J2219/00873 , B01L3/5027 , B01L7/5255 , B01L2200/14 , B01L2200/147 , B01L2300/1805 , B01L2300/1827 , B81B2201/051 , F28F21/02 , F28F2013/006
摘要： A fluid reaction device includes a microfluidic reaction chip which accommodates a fluid, a heater, and a heat transfer facilitating layer which is interposed between the microfluidic reaction chip and the heater, the heat transfer facilitating layer has a higher thermal conductivity than air and can hold particles, wherein formation of an air layer can be prevented.
摘要翻译：流体反应装置包括容纳流体的微流体反应芯片，加热器和介于微流体反应芯片和加热器之间的传热促进层，传热促进层具有比空气更高的导热性并且可以保持颗粒，其中可以防止空气层的形成。

4. 发明申请

US20080053647A1 METHOD OF REDUCING TEMPERATURE DIFFERENCE BETWEEN A PAIR OF SUBSTRATES AND FLUID REACTION DEVICE USING THE SAME 有权
标题翻译：降低基板对流与使用其的流体反应装置之间的温度差异的方法
公开(公告)号：US20080053647A1
公开(公告)日：2008-03-06
申请号：US11689640
申请日：2007-03-22
申请人： Kak Namkoong , Su-hyeon Kim , Jin-tae Kim , Chin-sung Park , Young-sun Lee
发明人： Kak Namkoong , Su-hyeon Kim , Jin-tae Kim , Chin-sung Park , Young-sun Lee
IPC分类号： F28F7/00
CPC分类号： B81B3/0081 , B01J19/0093 , B01J2219/00783 , B01J2219/00822 , B01J2219/00873 , B01L3/5027 , B01L7/5255 , B01L2200/14 , B01L2200/147 , B01L2300/1805 , B01L2300/1827 , B81B2201/051 , F28F21/02 , F28F2013/006
摘要： A method of reducing a temperature difference between a high-temperature and a low-temperature substrate includes interposing a heat transfer facilitating layer which has a higher thermal conductivity than air and can hold particles between the substrates, and maintaining close contact between the high-temperature substrate, the heat transfer facilitating layer, and the low-temperature substrate, wherein formation of an air layer can be at least substantially prevented between the high-temperature substrate and the heat transfer facilitating layer, and between the low-temperature substrate and the heat transfer facilitating layer. A fluid reaction device includes a microfluidic reaction chip which accommodates a fluid, a heater, and a heat transfer facilitating layer which is interposed between the microfluidic reaction chip and the heater, the heat transfer facilitating layer has a higher thermal conductivity than air and can hold particles, wherein formation of an air layer can be prevented.
摘要翻译：降低高温和低温基板之间的温度差的方法包括：介于热传导率高于空气的传热促进层，并且可以在基板之间保持颗粒，并且保持高温基板，传热促进层和低温基板，其中至少可以在高温基板和传热促进层之间以及在低温基板和热量之间基本上防止空气层的形成转移促进层。流体反应装置包括容纳流体的微流体反应芯片，加热器和介于微流体反应芯片和加热器之间的传热促进层，传热促进层具有比空气更高的导热性并且可以保持颗粒，其中可以防止空气层的形成。

5. 发明申请

US20090170189A1 MICROFLUIDIC DEVICE AND METHOD OF FABRICATING THE SAME 审中-公开
标题翻译：微流体装置及其制造方法
公开(公告)号：US20090170189A1
公开(公告)日：2009-07-02
申请号：US12184317
申请日：2008-08-01
申请人： Chin-sung PARK , Kak Namkoong , Young-sun Lee
发明人： Chin-sung PARK , Kak Namkoong , Young-sun Lee
IPC分类号： C12M1/34 , C12M1/00 , B32B37/06
CPC分类号： C12M23/16 , B01L2200/12 , B01L2300/0816 , B01L2300/1827 , B29C65/02 , B29C65/08 , B29C66/1122 , B29C66/30223 , B29C66/47 , B29C66/53461 , B29C66/61 , B29C66/71 , B29C66/7352 , B29C66/73921 , B29C66/8322 , B29K2023/06 , B29K2023/12 , B29K2033/12 , B29K2067/00 , B29K2069/00 , B29L2031/756 , Y10T156/10 , B29K2067/003 , B29K2023/38
摘要： Provided are a microfluidic device that performs a biochemical reaction using a small amount of a biochemical fluid and detects the result thereof, and a method of fabricating the same. The microfluidic device includes: a substrate which comprises a chamber that is formed as a concave groove and accommodates a fluid in the bottom surface of the substrate, and is formed of polymer; and a film welded on the bottom surface of the substrate to seal the chamber so that the chamber is not open at the bottom surface of the substrate, and formed of polymer. The method of fabricating a microfluidic device includes: preparing a substrate which comprises a chamber that is formed as a concave groove and accommodates a fluid in the bottom surface of the substrate, and is formed of polymer; and welding a film on a bottom surface of the substrate to seal the chamber so that the chamber is not opened at the bottom surface of the substrate, the film being formed of polymer.
摘要翻译：提供了使用少量生物化学流体进行生化反应并检测其结果的微流体装置及其制造方法。微流体装置包括：基板，其包括形成为凹槽并容纳基板的底表面中的流体的腔室，并且由聚合物形成; 以及焊接在基板的底表面上的膜以密封室，使得室不在基板的底表面处开口，并且由聚合物形成。制造微流体装置的方法包括：制备基板，其包括形成为凹槽的腔室，并且在基底的底表面容纳流体，并且由聚合物形成; 并且在所述基板的底表面上焊接膜以密封所述室，使得所述室在所述基板的底表面处不被打开，所述膜由聚合物形成。

6. 发明授权

US08278051B2 Method of amplifying a target nucleic acid by rolling circle amplification 有权
标题翻译：通过滚圆放大扩增靶核酸的方法
公开(公告)号：US08278051B2
公开(公告)日：2012-10-02
申请号：US13185003
申请日：2011-07-18
申请人： Joo-won Rhee , Young-sun Lee , Kak Namkoong , Chin-sung Park
发明人： Joo-won Rhee , Young-sun Lee , Kak Namkoong , Chin-sung Park
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6844 , C12Q1/6855 , C12Q2525/301 , C12Q2531/125 , C12Q2537/101 , C12Q2521/301 , C12Q2521/501
摘要： Provided is a method of amplifying a nucleic acid using rolling cyclic amplification (RCA), including amplifying a nucleic acid together with formation of a single-strand circular DNA template using RCA by reacting a reaction solution including: (a) two hairpin oligos, (b) a target nucleic acid, (c) a DNA ligase, (d) an endonuclease, (e) a DNA polymerase, and (f) a primer.
摘要翻译：提供了使用滚动循环扩增（RCA）扩增核酸的方法，包括使用RCA形成单链环状DNA模板来扩增核酸，反应溶液包括：（a）两个发夹寡核苷酸（ b）靶核酸，（c）DNA连接酶，（d）内切核酸酶，（e）DNA聚合酶和（f）引物。

7. 发明授权

US08185324B2 Method of determining initial concentration of nucleic acid in sample using real-time amplification data 有权
标题翻译：使用实时放大数据确定样品中核酸初始浓度的方法
公开(公告)号：US08185324B2
公开(公告)日：2012-05-22
申请号：US12259548
申请日：2008-10-28
申请人： Kak Namkoong , Young-sun Lee , Chin-sung Park , Joo-won Rhee
发明人： Kak Namkoong , Young-sun Lee , Chin-sung Park , Joo-won Rhee
IPC分类号： G01N33/48
CPC分类号： C12Q1/6851 , C12Q2537/165
摘要： Disclosed is a method of determining an initial concentration of a target nucleic acid within a sample using real-time nucleic acid amplification data. Amplification efficiencies of the target nucleic acid with respect to amplification time are obtained from signals of amplified products, and an amplification efficiency function with respect to amplification time is formulated employing the amplification efficiencies.
摘要翻译：公开了使用实时核酸扩增数据确定样品中靶核酸的初始浓度的方法。从放大产物的信号获得靶核酸相对于扩增时间的扩增效率，并且使用扩增效率来制定关于扩增时间的扩增效率函数。

8. 发明授权

US08003322B2 Method of amplifying a target nucleic acid by rolling circle amplification 有权
标题翻译：通过滚圆放大扩增靶核酸的方法
公开(公告)号：US08003322B2
公开(公告)日：2011-08-23
申请号：US12247433
申请日：2008-10-08
申请人： Joo-won Rhee , Young-sun Lee , Kak Namkoong , Chin-sung Park
发明人： Joo-won Rhee , Young-sun Lee , Kak Namkoong , Chin-sung Park
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6844 , C12Q1/6855 , C12Q2525/301 , C12Q2531/125 , C12Q2537/101 , C12Q2521/301 , C12Q2521/501
摘要： Provided is kit for and a method of amplifying a nucleic acid using rolling cyclic amplification (RCA), including amplifying a nucleic acid together with formation of a single-strand circular DNA template using RCA by reacting a reaction solution including: (a) two hairpin oligos, (b) a target nucleic acid, (c) a DNA ligase,(d) an endonuclease, (e) a DNA polymerase, and (f) a primer.
摘要翻译：提供了使用滚动循环扩增（RCA）扩增核酸的试剂盒和使用RCA形成单链环状DNA模板的核酸的方法，所述反应溶液包括：（a）两个发夹寡核苷酸，（b）靶核酸，（c）DNA连接酶，（d）内切核酸酶，（e）DNA聚合酶和（f）引物。

9. 发明申请

US20060047443A1 Method for quantifying initial concentration of nucleic acid from real-time nucleic acid amplification data 有权
公开(公告)号：US20060047443A1
公开(公告)日：2006-03-02
申请号：US11217694
申请日：2005-09-01
申请人： Kak Namkoong , Jin-tae Kim , Young-sun Lee , Young-a Kim
发明人： Kak Namkoong , Jin-tae Kim , Young-sun Lee , Young-a Kim
IPC分类号： G06F19/00 , C12Q1/68
CPC分类号： C12Q1/6851
摘要： Provided is a method for quantifying an initial concentration of a nucleic acid from a real-time nucleic acid amplification data. Nucleic acid (DNA or RNA) extracted from organism or virus is amplified using an enzyme. Then, the initial concentration of the nucleic acid is found by calculating the characteristic amplification cycle number or the characteristic amplification time at which the fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid has half of its maximum value, or the characteristic amplification cycle number or the characteristic amplification time at which the amplification efficiency has the maximum or the minimum value, or the prior-to-amplification fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid. Accordingly, the initial concentration of the nucleic acid can be calculated without differentiation or integration.

10. 发明授权

US08386191B2 Method for quantifying initial concentration of nucleic acid from real-time nucleic acid amplification data 有权
标题翻译：从实时核酸扩增数据定量核酸初始浓度的方法
公开(公告)号：US08386191B2
公开(公告)日：2013-02-26
申请号：US13161788
申请日：2011-06-16
申请人： Kak Namkoong , Jin-tae Kim , Young-sun Lee , Young-a Kim
发明人： Kak Namkoong , Jin-tae Kim , Young-sun Lee , Young-a Kim
IPC分类号： G01N33/48
CPC分类号： C12Q1/6851
摘要： Provided is a method for quantifying an initial concentration of a nucleic acid from a real-time nucleic acid amplification data. Nucleic acid (DNA or RNA) extracted from organism or virus is amplified using an enzyme. Then, the initial concentration of the nucleic acid is found by calculating the characteristic amplification cycle number or the characteristic amplification time at which the fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid has half of its maximum value, or the characteristic amplification cycle number or the characteristic amplification time at which the amplification efficiency has the maximum or the minimum value, or the prior-to-amplification fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid. Accordingly, the initial concentration of the nucleic acid can be calculated without differentiation or integration.
摘要翻译：提供了从实时核酸扩增数据定量核酸的初始浓度的方法。使用酶扩增从生物或病毒提取的核酸（DNA或RNA）。然后，通过计算由核酸的背景荧光强度减去的核酸的荧光强度具有其最大值的一半的特征扩增循环数或特异性扩增时间来发现核酸的初始浓度，或扩增效率具有由核酸的背景荧光强度减去的核酸的最大值或最小值或以前的扩增荧光强度的特征扩增循环数或特征扩增时间。因此，可以在不分化或整合的情况下计算核酸的初始浓度。

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