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    • 8. 发明授权
    • Fluorescence detector for detecting microfluid
    • 用于检测微流体的荧光检测器
    • US07327459B2
    • 2008-02-05
    • US10933084
    • 2004-09-02
    • Su-hyeon KimJin-tae Kim
    • Su-hyeon KimJin-tae Kim
    • G01N21/25
    • G01N21/645G01N2021/6482
    • An ultra small fluorescence detector capable of detecting in real time reaction undergoing in a micro chamber having a predetermined volume and disposed on a microfluid chip is provided. The fluorescence detector for detecting in real time PCR amplification undergoing in the microfluid chip having a micro chamber with a predetermined volume includes a light source generating an excitation beam, a first optical system capable of irradiating the excitation beam having a predetermined spot size to the micro chamber, a first detector, and a second optical system reflecting a fluorescent beam derived from the excitation beam having the predetermined spot size in the micro chamber to the first detector. Accordingly, the fluorescence detector is designed such that light emitted by a light source is focused between a first mirror and an objective lens. Therefore, the spot size of an excitation beam transmitted by the objective lens is largely formed so that the excitation beam can be irradiated on the whole micro chamber of the microfluid chip, thereby detecting a fluorescent beam on a broader area.
    • 提供了能够实时检测具有预定体积的微室中并设置在微流体芯片上的超小荧光检测器。 用于实时检测在具有预定体积的微室的微流体芯片中进行PCR扩增的荧光检测器包括产生激发光束的光源,能够将具有预定光斑尺寸的激发光束照射到微细的第一光学系统 第一检测器和第二光学系统,其将来自具有在微室中的预定光斑尺寸的激发光束的荧光束反射到第一检测器。 因此,荧光检测器被设计成使得由光源发射的光聚焦在第一反射镜和物镜之间。 因此,由物镜透射的激发光束的光斑尺寸大大地形成为使得激发光束可以照射在微流体芯片的整个微室上,从而在更广泛的区域上检测荧光束。