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1. 发明授权

US6015681A Rapid immunoassay for cariogenic bacteria 失效
标题翻译：快速免疫测定致龋细菌
公开(公告)号：US6015681A
公开(公告)日：2000-01-18
申请号：US766203
申请日：1996-12-12
申请人： Stephen Alden Ralls , Lloyd Grant Simonson
发明人： Stephen Alden Ralls , Lloyd Grant Simonson
IPC分类号： G01N33/569 , G01N33/554 , G01N33/53 , G01N33/537 , G01N33/543
CPC分类号： G01N33/56955 , G01N33/56944 , G01N2333/315 , G01N2333/335 , G01N2333/36 , Y10S435/975
摘要： An assay method and kit for detecting specific oral cariogenic bacteria, ., mutans streptococci, Lactobacillus sp. and Actinomyces sp., separately or in combination, comprising gathering a sample suspected of containing cariogenic bacteria; treating the sample with a stripping buffer to remove host antibodies from bacteria present in the sample; retaining the treated bacteria on a blocked solid phase substrate; reacting the retained bacteria with a primary antibody specific for the desired cariogenic bacteria; reacting the primary antibody with a conjugated label producing a detectable signal; and detecting the signal whereby the presence of the desired cariogenic bacteria is determined in the sample. The device for conducting these assays is a frame or support which holds a solid substrate capable of retaining the bacteria of interest while permitting drainage of other materials or fluids away from the retained bacteria.
摘要翻译：用于检测特定口腔致龋细菌的测定方法和试剂盒，例如变形链球菌，乳杆菌属（Lactobacillus sp。和放线菌属（Actinomyces sp。）分开或组合，包括收集怀疑含有致龋细菌的样品; 用剥离缓冲液处理样品以除去样品中存在的细菌的宿主抗体; 将经处理的细菌保留在封闭的固相底物上; 使所保留的细菌与所需致龋细菌特异性的一级抗体反应; 使一次抗体与产生可检测信号的共轭标记反应; 并检测信号，由此在样品中确定所需致龋菌的存在。用于进行这些测定的装置是一种框架或支撑体，其保持固体基底，其能够保留感兴趣的细菌，同时允许其它材料或流体从保留的细菌排出。

2. 发明授权

US5741659A Rapid microbial protease assay 失效
标题翻译：快速微生物蛋白酶测定
公开(公告)号：US5741659A
公开(公告)日：1998-04-21
申请号：US583170
申请日：1996-01-04
申请人： Stephen Alden Ralls , Lloyd Grant Simonson , Sylvia Zottu Schade
发明人： Stephen Alden Ralls , Lloyd Grant Simonson , Sylvia Zottu Schade
IPC分类号： C12Q1/37 , A01N37/18 , C12Q1/04 , C12Q1/52
CPC分类号： C12Q1/37 , C12Q2337/12
摘要： An assay for detecting microbial protease activity in clinical and laboray samples is described which comprises gathering a sample suspected of containing certain microorganisms having the desired protease activity; immobilizing the microorganisms in the sample on a solid phase substrate; contacting the immobilized microorganisms with an enzymatic substrate producing an enzymatic substrate end-product; contacting the enzymatic substrate end-product with a chemical enhancing reagent producing a detectable chromogenic reaction which varies in intensity with the level of protease activity in the sample; and detecting the chromogenic reaction whereby the semi-quantitative presence of the protease activity in the sample is determined. The device for conducting these assays is a frame or support which holds a solid phase substrate capable of binding the sought microorganisms of interest while permitting drainage of other materials or fluids, which may contain host proteases, away from the immobilized microorganisms.
摘要翻译：描述了用于检测临床和实验室样品中的微生物蛋白酶活性的测定法，其包括收集怀疑含有具有所需蛋白酶活性的某些微生物的样品; 将样品中的微生物固定在固相基质上; 使固定的微生物与产生酶底物最终产物的酶底物接触; 使酶底物最终产物与化学增强剂接触，产生可检测的显色反应，其强度随样品中蛋白酶活性的水平而变化; 并检测显色反应，由此确定样品中蛋白酶活性的半定量存在。用于进行这些测定的装置是保持固相基质能够结合目的的所需微生物的框架或载体，同时允许其它材料或可能含有宿主蛋白酶的流体从固定的微生物排出。

3. 发明授权

US06599691B1 Rapid immunoassay to detect infection with Mycobacterium tuberculosis 失效
标题翻译：快速免疫分析法检测结核分枝杆菌感染
公开(公告)号：US06599691B1
公开(公告)日：2003-07-29
申请号：US09044214
申请日：1997-02-24
申请人： Stephen Alden Ralls , Lloyd Grant Simonson
发明人： Stephen Alden Ralls , Lloyd Grant Simonson
IPC分类号： C12Q100
CPC分类号： G01N33/5695
摘要： A rapid, non-invasive, semi-quantitative immunoassay of saliva has been developed to aid in the diagnosis of diseases, e.g., using saliva to detect subjects actively or previously infected with Mycobacterium tuberculosis, a causative organism of tuberculosis. The semi-quantitative assay comprises spotting disease-related antigens on the surface of a solid substrate; contacting the solid substrate with a saliva sample which, in positive subjects, contains primary antibodies to the disease-related antigens; contacting the primary antibodies with a label capable of being detected; and detecting and reading the label whereby exposure to the antigens is determined. The device for conducting these assays is a frame or support which holds a solid substrate capable of immobilizing the antigens of interest while permitting drainage of other materials or fluids away from the immobilized antigens. A less rapid, quantitative assay has also been developed by adapting the rapid, semi-quantitative assay to an enzyme linked immunosorbant assay thereby providing a quantitative assay capable of assessing multiple saliva samples simultaneously.
摘要翻译：已经开发了唾液的快速，非侵入性，半定量免疫测定法，以帮助诊断疾病，例如使用唾液来主动地或以前感染结核分枝杆菌的致病微生物结核分枝杆菌。半定量测定法包括在固体底物的表面上检测疾病相关的抗原; 使固体基质与唾液样品接触，唾液样品在阳性受试者中含有与疾病相关抗原的一抗; 使一次抗体与能够被检测的标记接触; 并且检测和阅读标签，由此确定暴露于抗原。用于进行这些测定的装置是框架或支撑体，其保持固体基底，其能够固定感兴趣的抗原，同时允许其它材料或流体从固定的抗原排出。通过将快速，半定量测定法适应于酶联免疫吸附测定法也开发了不太快速的定量测定法，从而提供能够同时评估多种唾液样品的定量测定。

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