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1. 发明授权

US09273174B2 Nucleic acid detection method, and device and kit for use in same 有权
标题翻译：核酸检测方法，以及用于其中的装置和试剂盒
公开(公告)号：US09273174B2
公开(公告)日：2016-03-01
申请号：US14002886
申请日：2012-03-05
申请人： Shigehiko Miyamoto , Jun Tomono , Koji Takahashi , Sotaro Sano , Takaaki Jikihara
发明人： Shigehiko Miyamoto , Jun Tomono , Koji Takahashi , Sotaro Sano , Takaaki Jikihara
IPC分类号： C12Q1/68 , G01N21/78 , C08F222/10 , G01N21/03
CPC分类号： G01N21/78 , C08F222/10 , C12Q1/6806 , C12Q1/6846 , C12Q1/6848 , G01N21/03 , Y10T436/143333 , C12Q2547/101 , C12Q2563/103 , C12Q2563/173
摘要： The present invention aims to solve problems in the analysis of amplified nucleic acids, i.e., cost, workability, and contamination and pollution of samples. In the present invention, a sample containing a nucleic acid and a reagent for detecting the nucleic acid are mixed in a closed system. In this regard, the nucleic acid is amplified in the same closed system, and then mixed with the detecting reagent without opening the system. In order to carry out this method, for example, a device that includes the detecting reagent shielded by a coating material or the like is used.
摘要翻译：本发明旨在解决扩增核酸分析中的问题，即成本，可加工性以及样品的污染和污染。在本发明中，含有核酸和检测核酸的试剂的样品在封闭系统中混合。在这方面，核酸在相同的封闭系统中扩增，然后与检测试剂混合而不打开系统。为了实施该方法，例如，使用包含被涂料等遮蔽的检测试剂的装置。

2. 发明授权

US09097718B2 Method and device for detecting nucleic acid, and kit 有权
标题翻译：用于检测核酸的方法和装置，以及试剂盒
公开(公告)号：US09097718B2
公开(公告)日：2015-08-04
申请号：US13823946
申请日：2011-09-21
申请人： Shigehiko Miyamoto , Takaaki Jikihara , Sotaro Sano , Koji Takahashi , Jun Tomono
发明人： Shigehiko Miyamoto , Takaaki Jikihara , Sotaro Sano , Koji Takahashi , Jun Tomono
IPC分类号： G01N33/58 , G01N21/78 , C12Q1/68
CPC分类号： G01N33/583 , C12Q1/6816 , C12Q1/6846 , C12Q1/686 , G01N21/78 , Y10T436/143333 , C12Q2563/173
摘要： The present invention provides a method for detecting a nucleic acid, by which a multi-stranded nucleic acid amplified by a nucleic acid amplification method is detected easily and with a high degree of accuracy without the need for specialized equipment, and also provides a nucleic acid detection device. Provided are a method for detecting a nucleic acid under visible light via a color reaction produced by contact between a chromogenic leuco dye and the multi-stranded nucleic acid, as well as a nucleic acid detection device using this method.
摘要翻译：本发明提供一种检测核酸的方法，通过该方法可以容易地且高精度地检测通过核酸扩增方法扩增的多链核酸而不需要专门的设备，并且还提供核酸检测装置提供了通过由显色无色染料和多链核酸之间的接触产生的显色反应以及使用该方法的核酸检测装置在可见光下检测核酸的方法。

3. 外观设计

USD725286S1 Combined micro tube and cap 有权
标题翻译：组合微管和帽
公开(公告)号：USD725286S1
公开(公告)日：2015-03-24
申请号：US29425581
申请日：2012-06-25
申请人： Shigehiko Miyamoto , Jun Tomono , Sotaro Sano
设计人： Shigehiko Miyamoto , Jun Tomono , Sotaro Sano

4. 发明申请

US20140058043A1 NUCLEIC ACID DETECTION METHOD, AND DEVICE AND KIT FOR USE IN SAME 有权
标题翻译：核酸检测方法，以及用于其的装置和试剂盒
公开(公告)号：US20140058043A1
公开(公告)日：2014-02-27
申请号：US14002886
申请日：2012-03-05
申请人： Shigehiko Miyamoto , Jun Tomono , Koji Takahashi , Sotaro Sano , Takaaki Jikihara
发明人： Shigehiko Miyamoto , Jun Tomono , Koji Takahashi , Sotaro Sano , Takaaki Jikihara
IPC分类号： C08F222/10
CPC分类号： G01N21/78 , C08F222/10 , C12Q1/6806 , C12Q1/6846 , C12Q1/6848 , G01N21/03 , Y10T436/143333 , C12Q2547/101 , C12Q2563/103 , C12Q2563/173
摘要： The present invention aims to solve problems in the analysis of amplified nucleic acids, i.e., cost, workability, and contamination and pollution of samples. In the present invention, a sample containing a nucleic acid and a reagent for detecting the nucleic acid are mixed in a closed system. In this regard, the nucleic acid is amplified in the same closed system, and then mixed with the detecting reagent without opening the system. In order to carry out this method, for example, a device that includes the detecting reagent shielded by a coating material or the like is used.
摘要翻译：本发明旨在解决扩增核酸分析中的问题，即成本，可加工性以及样品的污染和污染。在本发明中，含有核酸和检测核酸的试剂的样品在封闭系统中混合。在这方面，核酸在相同的封闭系统中扩增，然后与检测试剂混合而不打开系统。为了实施该方法，例如，使用包含被涂料等遮蔽的检测试剂的装置。

5. 发明申请

US20130252341A1 METHOD AND DEVICE FOR DETECTING NUCLEIC ACID, AND KIT 有权
标题翻译：用于检测核酸和方法的方法和装置
公开(公告)号：US20130252341A1
公开(公告)日：2013-09-26
申请号：US13823946
申请日：2011-09-21
申请人： Shigehiko Miyamoto , Takaaki Jikihara , Sotaro Sano , Koji Takahashi , Jun Tomono
发明人： Shigehiko Miyamoto , Takaaki Jikihara , Sotaro Sano , Koji Takahashi , Jun Tomono
IPC分类号： G01N33/58
CPC分类号： G01N33/583 , C12Q1/6816 , C12Q1/6846 , C12Q1/686 , G01N21/78 , Y10T436/143333 , C12Q2563/173
摘要： The present invention provides a method for detecting a nucleic acid, by which a multi-stranded nucleic acid amplified by a nucleic acid amplification method is detected easily and with a high degree of accuracy without the need for specialized equipment, and also provides a nucleic acid detection device. Provided are a method for detecting a nucleic acid under visible light via a color reaction produced by contact between a chromogenic leuco dye and the multi-stranded nucleic acid, as well as a nucleic acid detection device using this method.
摘要翻译：本发明提供一种检测核酸的方法，通过该方法可以容易地且高精度地检测通过核酸扩增方法扩增的多链核酸而不需要专门的设备，并且还提供核酸检测装置提供了通过由显色无色染料和多链核酸之间的接触产生的显色反应以及使用该方法的核酸检测装置在可见光下检测核酸的方法。

6. 发明申请

US20110117549A1 METHOD FOR DETECTING NUCLEIC ACID, AND DEVICE OR KIT 有权
标题翻译：检测核酸的方法，以及装置或试剂盒
公开(公告)号：US20110117549A1
公开(公告)日：2011-05-19
申请号：US12726680
申请日：2010-03-18
申请人： Shigehiko Miyamoto , Tomohisa Kato , Koji Takahashi , Jun Tomono
发明人： Shigehiko Miyamoto , Tomohisa Kato , Koji Takahashi , Jun Tomono
IPC分类号： G01N33/52 , C12M1/34
CPC分类号： G01N21/78 , C12Q1/6816 , G01N33/52 , G01N33/5308 , C12Q2563/173 , C12Q2537/101 , C12Q2563/137
摘要： The present invention has its object to provide a method and a device or kit for detecting a nucleic acid, which enable simple and precise visual detection of a nucleic acid amplified by a nucleic acid amplification method, without necessity of a special device.The present invention relates to a method for detecting a nucleic acid in a sample, comprising the steps of: (1) contacting a sample with a dye to react with each other; and (2) observing a substance produced by the reaction with visible light, and evaluating the presence of a nucleic acid by eye. The present invention also relates to a device or kit for detecting a nucleic acid in a sample, comprising: a carrier (a) that holds a dye which can bind to a nucleic acid; a path (c) for passing a sample through (a) the carrier; and an evaluation part (d) for observing a substance produced by the reaction between the sample and the dye with visible light, and evaluating the presence of a nucleic acid by eye.
摘要翻译：本发明的目的是提供用于检测核酸的方法和装置或试剂盒，其能够简单且精确地通过核酸扩增方法扩增的核酸的视觉检测，而不需要特殊的装置。本发明涉及一种用于检测样品中核酸的方法，包括以下步骤：（1）使样品与染料接触以进行反应; 和（2）观察通过可见光反应产生的物质，并通过眼睛评估核酸的存在。本发明还涉及用于检测样品中的核酸的装置或试剂盒，其包含：载体（a），其保持可与核酸结合的染料; 用于使样品通过（a）载体的路径（c）; 以及用可见光观察由样品和染料之间的反应产生的物质的评价部分（d），并且通过眼睛评估核酸的存在。

7. 发明申请

US20140065725A1 AMPLIFIED NUCLEIC ACID DETECTION METHOD AND DETECTION DEVICE 有权
标题翻译：放大核酸检测方法和检测装置
公开(公告)号：US20140065725A1
公开(公告)日：2014-03-06
申请号：US13989207
申请日：2011-11-24
申请人： Koji Takahashi , Shigehiko Miyamoto , Takaaki Jikihara , Jun Tomono
发明人： Koji Takahashi , Shigehiko Miyamoto , Takaaki Jikihara , Jun Tomono
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6834 , C12Q1/6832 , C12Q1/686 , G01N33/54313 , C12Q2525/155 , C12Q2525/161 , C12Q2565/519 , C12Q2525/186 , C12Q2531/113 , C12Q2565/625
摘要： An object of the invention is to provide a nucleic acid detection method which takes advantage of the high specificity of hybridization techniques, reduces the time length and the number of steps required for detection of PCR products, and allows for easy and highly accurate detection by visual observation without the need of special equipment; and a nucleic acid detection device or kit. The invention provides a method for detecting a target nucleic acid in a sample, which includes performing amplification of the target nucleic acid sequence to synthesize an amplification product having a partially double-stranded structure where a single-stranded region is added to each end of the target sequence, and hybridizing a nucleic acid sequence bound to a development medium and a nucleic acid sequence labeled with a labeling compound with the single-stranded regions of the amplification product to form a sandwich hybridization complex; and a detection device thereof.
摘要翻译：本发明的目的是提供一种核酸检测方法，其利用杂交技术的高特异性，减少检测PCR产物所需的时间长度和数量，并且允许通过视觉方便和高度准确的检测观察无需特殊设备; 和核酸检测装置或试剂盒。本发明提供了一种用于检测样品中靶核酸的方法，其包括进行目标核酸序列的扩增以合成具有部分双链结构的扩增产物，其中单链区域被添加到靶序列，并将与显影培养基结合的核酸序列与标记化合物标记的核酸序列与扩增产物的单链区域杂交以形成夹心杂交复合物; 及其检测装置。

8. 发明授权

US08546131B2 Method for detecting nucleic acid, and device or kit 有权
标题翻译：检测核酸的方法，以及装置或试剂盒
公开(公告)号：US08546131B2
公开(公告)日：2013-10-01
申请号：US12726680
申请日：2010-03-18
申请人： Shigehiko Miyamoto , Tomohisa Kato , Koji Takahashi , Jun Tomono
发明人： Shigehiko Miyamoto , Tomohisa Kato , Koji Takahashi , Jun Tomono
IPC分类号： C12M1/34 , C12M3/00 , G01N15/06 , C07H21/02 , C07H21/04
CPC分类号： G01N21/78 , C12Q1/6816 , G01N33/52 , G01N33/5308 , C12Q2563/173 , C12Q2537/101 , C12Q2563/137
摘要： A method and a device or kit for detecting a nucleic acid, which enable simple and precise visual detection of a nucleic acid amplified by an nucleic acid amplification method, without necessity of special devices are provided. The method for detecting a nucleic acid in a sample comprises: contacting a sample with a dye to react with each other; and observing a substance produced by the reaction with visible light, and evaluating the presence or absence of a nucleic acid by eye. The device or kit for detecting a nucleic acid in a sample comprises: a carrier that holds a dye which can bind to a nucleic acid; a path for passing a sample through the carrier; and an evaluation part for observing a substance produced by the reaction between the sample and the dye with visible light, and evaluating the presence or absence of a nucleic acid by eye.
摘要翻译：提供了用于检测核酸的方法和装置或试剂盒，其能够简单且精确地目视检测通过核酸扩增方法扩增的核酸，而不需要特殊的装置。用于检测样品中核酸的方法包括：使样品与染料接触以进行反应; 并观察通过可见光反应产生的物质，并通过眼睛评估核酸的存在或不存在。用于检测样品中的核酸的装置或试剂盒包括：载持可以结合核酸的染料的载体; 将样品通过载体的路径; 以及用于用可见光观察由样品和染料之间的反应产生的物质的评估部分，并且通过眼睛评估核酸的存在或不存在。

9. 发明授权

US09920356B2 Amplified nucleic acid detection method and detection device 有权
公开(公告)号：US09920356B2
公开(公告)日：2018-03-20
申请号：US13989207
申请日：2011-11-24
申请人： Koji Takahashi , Shigehiko Miyamoto , Takaaki Jikihara , Jun Tomono
发明人： Koji Takahashi , Shigehiko Miyamoto , Takaaki Jikihara , Jun Tomono
IPC分类号： C12P19/34 , C12Q1/68 , C07H21/02 , C07H21/04 , C07H21/00 , G01N33/543
CPC分类号： C12Q1/6834 , C12Q1/6832 , C12Q1/686 , G01N33/54313 , C12Q2525/155 , C12Q2525/161 , C12Q2565/519 , C12Q2525/186 , C12Q2531/113 , C12Q2565/625
摘要： An object of the invention is to provide a nucleic acid detection method which takes advantage of the high specificity of hybridization techniques, reduces the time length and the number of steps required for detection of PCR products, and allows for easy and highly accurate detection by visual observation without the need of special equipment; and a nucleic acid detection device or kit. The invention provides a method for detecting a target nucleic acid in a sample, which includes performing amplification of the target nucleic acid sequence to synthesize an amplification product having a partially double-stranded structure where a single-stranded region is added to each end of the target sequence, and hybridizing a nucleic acid sequence bound to a development medium and a nucleic acid sequence labeled with a labeling compound with the single-stranded regions of the amplification product to form a sandwich hybridization complex; and a detection device thereof.

10. 发明授权

US4620197A Ink jet recording method 失效
标题翻译：喷墨记录方法
公开(公告)号：US4620197A
公开(公告)日：1986-10-28
申请号：US532512
申请日：1983-09-15
申请人： Shigehiko Miyamoto , Yoshinobu Watanabe , Yuriko Kishi
发明人： Shigehiko Miyamoto , Yoshinobu Watanabe , Yuriko Kishi
IPC分类号： B41M5/00 , B41M5/50 , B41M5/52 , C09D11/00 , D21H19/10 , G01D15/18 , G01D15/16 , B05D5/00 , B32B7/00
CPC分类号： G01D15/18 , B41M5/5227 , B41M5/5245 , C09D11/328 , Y10T428/24893 , Y10T428/24901 , Y10T428/259
摘要： By allowing at least the surface of an ink jet recording sheet to contain a cationic fluorescent agent, the waterproofness of water-soluble dyes in inks as well as the brightness of the recording sheet can be enhanced and thereby multicolor images of excellent color reproducibility can be formed on the sheet.
摘要翻译：通过使至少喷墨记录纸的表面含有阳离子荧光剂，可以提高油墨中水溶性染料的防水性和记录片材的亮度，从而可以提高色彩再现性优异的多色图像形成在纸上。

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