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    • 3. 发明申请
    • Cloning of rhadinovirus genome and methods for its use
    • 克隆野毒病毒基因组及其使用方法
    • US20050239052A1
    • 2005-10-27
    • US10276524
    • 2001-05-17
    • Scott WongMichael Axthelm
    • Scott WongMichael Axthelm
    • A01K67/027A61K39/12A61K39/245A61K48/00C07H21/04C07K14/005C07K14/03C07K16/08C12N7/00C12N15/86C12Q1/70
    • C07K14/005A01K67/027A01K2227/106A01K2267/0325A01K2267/0337A61K39/12A61K39/245A61K48/00A61K2039/525C07K16/085C12N7/00C12N2710/16022C12N2710/16421C12N2710/16422C12N2710/16432C12N2710/16434C12Q1/705G01N2333/03
    • An isolated virus is provided (Japanese Macaque Herpesvirus, JMHV), as deposited with ATCC as deposit accession number PTA-1884, as are viral particles including this virus and host cells infected with this virus. A purified polypeptide is also provided that includes an amino acid sequence that has at least 95% sequence identity to an amino acid sequence as set forth as SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:25, and SEQ ID NO:27, as are nucleic acid molecules encoding these polypeptides. A method is provided for testing the efficacy of a drug in the treatment of a condition associated with infection with JMHV. A model for multiple sclerosis is provided, as is a method for testing the efficacy of a candidate vaccine against JMHV infection, or conditions associated with JMHV infection. In a further embodiment, a method is provided for detecting the presence of JMHV or a related virus in a biological specimen, by amplifying by polymerase chain reaction a JMHV nucleic acid sequence, or by using hybridization technology, if such sequence is present in the sample. A method is also provided for detecting the presence of JMHV in a biological specimen. Kits are provided including an antibody that binds to a JMHV polypeptide or an oligonucleotide that hybridizes to a JMHV nucleic acid sequence.
    • 提供了分离的病毒(日本猕猴疱疹病毒,JMHV),其保藏为ATCC作为保藏登录号PTA-1884,以及包括该病毒的病毒颗粒和感染该病毒的宿主细胞。 还提供了纯化的多肽,其包括与SEQ ID NO:3,SEQ ID NO:5,SEQ ID NO:8,SEQ ID NO:1所示的氨基酸序列具有至少95%的序列同一性的氨基酸序列 :10,SEQ ID NO:12,SEQ ID NO:14,SEQ ID NO:17,SEQ ID NO:19,SEQ ID NO:21,SEQ ID NO:23,SEQ ID NO:25和SEQ ID NO: 27,编码这些多肽的核酸分子也是如此。 提供了一种用于测试药物治疗与JMHV感染相关的病症的功效的方法。 提供了多发性硬化的模型,以及用于测试候选疫苗针对JMHV感染或与JMHV感染相关的病症的功效的方法。 在另一个实施方案中,提供了用于通过JMHV核酸序列的聚合酶链反应扩增或通过杂交技术来检测生物样本中JMHV或相关病毒的存在的方法,如果该序列存在于样品中 。 还提供了用于检测生物样品中JMHV的存在的方法。 提供试剂盒,包括结合JMHV多肽的抗体或与JMHV核酸序列杂交的寡核苷酸。